scholarly journals The Inhibitory Effect of Sulforaphane on Bladder Cancer Cell Depends on GSH Depletion-Induced by Nrf2 Translocation

Molecules ◽  
2021 ◽  
Vol 26 (16) ◽  
pp. 4919
Author(s):  
Canxia He ◽  
Luigina P. Buongiorno ◽  
Wei Wang ◽  
Jonathan C. Y. Tang ◽  
Natalizia Miceli ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Inhibitory Effect ◽  
Bladder Cancer Cell ◽  
High Dose ◽  
Cell Growth And Migration ◽  
T24 Cells ◽  
And Migration

Sulforaphane (SFN), an isothiocyanate (ITCs) derived from glucosinolate that is found in cruciferous vegetables, has been reported to exert a promising anticancer effect in a substantial amount of scientific research. However, epidemical studies showed inconsistencies between cruciferous vegetable intake and bladder cancer risk. In this study, human bladder cancer T24 cells were used as in vitro model for revealing the inhibitory effect and its potential mechanism of SFN on cell growth. Here, a low dose of SFN (2.5 µM) was shown to promote cell proliferation (5.18–11.84%) and migration in T24 cells, whilst high doses of SFN (>10 µM) inhibited cell growth significantly. The induction effect of SFN on nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression at both low (2.5 µM) and high dose (10 µM) was characterized by a bell-shaped curve. Nrf2 and glutathione (GSH) might be the underlying mechanism in the effect of SFN on T24 cell growth since Nrf2 siRNA and GSH-depleting agent L-Buthionine-sulfoximine abolished the effect of SFN on cell proliferation. In summary, the inhibitory effect of SFN on bladder cancer cell growth and migration is highly dependent on Nrf2-mediated GSH depletion and following production. These findings suggested that a higher dose of SFN is required for the prevention and treatment of bladder cancer.

scholarly journals Transcriptional cofactor Mask2 is required for YAP-induced cell growth and migration in bladder cancer cell

2016 ◽  
Vol 7 (14) ◽  
pp. 2132-2138 ◽  
Author(s):  
Liang Dong ◽  
Fan Lin ◽  
Wanjun Wu ◽  
Weiren Huang ◽  
Zhiming Cai
Keyword(s):  
Bladder Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Bladder Cancer Cell ◽  
Cell Growth And Migration ◽  
And Migration

scholarly journals Qici Sanling Decoction Suppresses Glutamine Consumption and Bladder Cancer Cell Growth through Inhibiting c-Myc Expression

2022 ◽  
Vol 2022 ◽  
pp. 1-9
Author(s):  
Weihua Chen ◽  
Weifeng Wang ◽  
Jun Zhang ◽  
Guoqiang Liao ◽  
Jie Bai ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Alternative Therapy ◽  
Bladder Cancer Cell ◽  
Dependent Manner ◽  
Cancer Cell Growth ◽  
Protein Levels ◽  
T24 Cells ◽  
Glutamine Consumption

Traditional Chinese medicine (TCM) is widely used as an alternative therapy for cancer treatment in China. Glutamine catabolism plays an important role in cancer development. Qici Sanling decoction (QCSL) suppresses bladder cancer growth. However, the association between QCSL and glutamine catabolism remains unknown. In this study, different doses of QCSL were applied to T24 cells, followed by the measurements of cell viability and apoptosis using CCK-8 and Annexin V/PI assay, respectively. Furthermore, glutamine consumption was detected using the glutamine assay kit. QCSL was observed to inhibit cell growth and induced cell apoptosis in a dose-dependent manner. Analysis of glutamine consumption revealed that QCSL suppressed glutamine consumption in T24 cells. Furthermore, QCSL decreased the mRNA and protein levels of c-Myc, GLS1, and SLC1A5. All these effects induced by QCSL could be alleviated by c-Myc overexpression, indicating c-Myc was involved in the protective role of QCSL in bladder cancer. In addition, QCSL was found to inhibit tumor growth in the xenograft tumor model. The similar results were obtained in tumor samples that protein levels of c-Myc, GLS1, and SLC1A5 were decreased upon treatment with QCSL. In conclusion, QCSL suppresses glutamine consumption and bladder cancer cell growth through inhibiting c-Myc expression.

KLF8 is required for bladder cancer cell proliferation and migration

2015 ◽  
Vol 62 (5) ◽  
pp. 628-633 ◽  
Author(s):  
Kai Liang ◽  
Tao Liu ◽  
Ning Chu ◽  
Jian Kang ◽  
Rui Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cancer Cell ◽  
Bladder Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals Geraniin inhibits bladder cancer cell growth via regulation of PI3K/AKT signaling pathways

2020 ◽  
Vol 19 (2) ◽  
pp. 253-257
Author(s):  
Junwei Xu ◽  
Ning Qin ◽  
Yebin Yao ◽  
Tao Chen ◽  
Wenbo Jiang
Keyword(s):  
Bladder Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Akt Signaling ◽  
Bladder Cancer Cell ◽  
Akt Pathway ◽  
Cancer Cell Growth ◽  
Akt Phosphorylation ◽  
T24 Cells ◽  
Thiazolyl Blue Tetrazolium Bromide

Purpose: The effect of geraniin on human bladder transitional carcinoma was not clear, this study was thus intended to reveal it and reveal the mechanism. Methods: T24 cells were treated with 5, 10, and 20 μM of geraniin and the viability and apoptosis of T24 cells were determined using thiazolyl blue tetrazolium bromide (MTT) assay and flow cytometry. The protein expression levels of Cyclin D1, p21, BAL-2, BAX, cleaved caspase-3 and PI3K/AKT pathway were evaluated using western blot. Results: Geraniin decreased T24 cell viability and induced T24 cell cycle arrest. The proportion of T24 cells in S phase was decreased by geraniin. Besides, geraniin promoted T24 cell apoptosis and regulated PI3K/AKT pathway. Conclusion: Geraniin appears to regulate bladder cancer cell growth by decreasing the levels of PI3K and AKT phosphorylation. Thus, this agent may be useful in the management of bladder cancer Keywords: Geraniin, T24 cells, Apoptosis, PI3K/AKT signaling

scholarly journals TBK1 Promote Bladder Cancer Cell Proliferation and Migration via Akt Signaling

2017 ◽  
Vol 8 (10) ◽  
pp. 1892-1899 ◽  
Author(s):  
Wei Chen ◽  
Kewang Luo ◽  
Zhiyi ke ◽  
Bin Kuai ◽  
Shiyang He ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cancer Cell ◽  
Akt Signaling ◽  
Bladder Cancer Cell ◽  
Cancer Cell Proliferation ◽  
Cell Proliferation And Migration ◽  
And Migration ◽  
Proliferation And Migration

scholarly journals CCDC34 is up-regulated in bladder cancer and regulates bladder cancer cell proliferation, apoptosis and migration

Oncotarget ◽  
2015 ◽  
Vol 6 (28) ◽  
pp. 25856-25867 ◽  
Author(s):  
Yanqing Gong ◽  
Wei Qiu ◽  
Xianghui Ning ◽  
Xinyu Yang ◽  
Libo Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bladder Cancer ◽  
Cancer Cell ◽  
Bladder Cancer Cell ◽  
Cancer Cell Proliferation ◽  
And Migration
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