scholarly journals Bio-Guided Fractionation of Retama raetam (Forssk.) Webb & Berthel Polar Extracts

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 5800
Author(s):  
Mariem Saada ◽  
Hanen Wasli ◽  
Inès Jallali ◽  
Rim Kboubi ◽  
Karl Girard-Lalancette ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
Polar Compounds ◽  
Bioactive Molecules ◽  
Prenylated Flavonoids ◽  
Retama Raetam ◽  
Polar Extracts ◽  
Layer Chromatography

The fractionation of the methanolic extract (MeOH-E) of Retama raetam (Forssk.) Webb & Berthel and further analysis by thin layer chromatography resulted in four fractions (F1, F2, F3 and F4) that, in parallel with the MeOH-E, were screened for antioxidant, cytotoxic, antidiabetic and antibacterial properties. In addition, chemical characterization of their bioactive molecules was performed using LC-DAD-ESI/MSn. The results indicated that F3 was the most promising regarding antioxidant and cytotoxicity abilities, possibly due to its richness in flavonoids class, particularly isoflavones. In turn, F1 was characterized by the presence of the most polar compounds from MeOH-E (organic acids and piscidic acid) and showed promising abilities to inhibit α-amylase, while F4, which contained prenylated flavonoids and furanoflavonoids, was the most active against the tested bacteria. The gathered results emphasize the distinct biological potentials of purified fractions of Retama raetam.

scholarly journals Anti-Trypanosoma cruzi activity, cytotoxicity and, chemical characterization of extracts from seeds of Lonchocarpus cultratus

2021 ◽  
Vol 15 (02) ◽  
pp. 270-279
Author(s):  
Aline Griebler ◽  
Fernanda Weyand Banhuk ◽  
Izabela Virginia Staffen ◽  
Aline Antunes Maciel Bortoluzzi ◽  
Thaís Soprani Ayala ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Methanolic Extract ◽  
Chemical Characterization ◽  
Peritoneal Macrophages ◽  
Chemical Profile ◽  
Murine Macrophages ◽  
Human Red Blood Cells

Introduction: Trypanosoma cruzi is the agent of Chagas’ disease and affects approximately 6-8 million people worldwide. The search for new anti-T. cruzi drugs are relevant because only two drugs exist actually. The objective of this study was to investigate the effect of the extracts from the seeds of Lonchocarpus cultratus on T. cruzi, its cytotoxicity as well as to elucidate its chemical profile. Methodology: The characterization of the extracts was done using 1H-RMN. T. cruzi forms were treated with increasing concentrations of the extracts and after, the percentage of inhibition and IC50 or LC50 were calculated. Murine peritoneal macrophages were treated with different concentrations of the extracts to evaluate the cellular viability. The hemotoxicity was accessed by verifying the levels of hemolysis caused by the extracts on human red blood cells. Results: Chalcones isocordoin and lonchocarpin were detected in the dichloromethane extract, and chalcone lonchocarpin was detected in the hexane extract. The dichloromethane extract showed higher activity against all the forms of T. cruzi compared to the other two extracts, but the hexane showed the best selectivity index. The cytotoxicity observed in murine macrophages was confirmed in human erythrocytes, with dichloromethane extract having the highest toxicity. The methanolic extract showed the lowest anti-T. cruzi activity but was nontoxic to peritoneal murine macrophages and red blood cells. Conclusions: L. cultratus extracts have the potential to be explored for the development of new anti-trypanosomal drugs. This study was the first to demonstrate the action of extracts from the genus Lonchocarpus on infecting forms of T. cruzi.

Chemical characterization of the bark of Eucalyptus urophylla hybrids in view of their valorization in biorefineries

Holzforschung ◽  
2016 ◽  
Vol 70 (9) ◽  
pp. 819-828 ◽  
Author(s):  
Caroline Sartori ◽  
Graciene da Silva Mota ◽  
Joana Ferreira ◽  
Isabel Miranda ◽  
Fábio Akira Mori ◽  
...  
Keyword(s):  
Phenolic Content ◽  
Raw Materials ◽  
Chemical Characterization ◽  
Polar Compounds ◽  
Added Value ◽  
Bark Extract ◽  
Eucalyptus Urophylla ◽  
Acid Hydrolysate ◽  
Before And After

Abstract Eucalyptus urophylla hybrids are important raw materials for the forest industry in Brazil, and large quantities of barks are available at mill site that may be used for added-value products. The chemical composition of barks of six commercial hybrids clones of E. urophylla×E. grandis, E. urophylla×E. Camaldulensis, and undisclosed E. urophylla hybrids was studied. The hybrids had similar composition, on average (data based on oven dry bark): 16% extractives, mainly corresponding to polar compounds that are soluble in ethanol and water, 19% lignin, 47% polysaccharides, 1% suberin, and 2% ash. The polysaccharides consists mainly of cellulose as indicated by 84% of total neutral monosaccharides in the acid hydrolysate and 10% xylose. The compositions of the lipophilic extracts was determined by GC-MS before and after alkaline hydrolysis. In all the barks, fatty acids, and triterpenes (namely: betulinic and ursolic acids) were the most abundant compounds followed by smaller amounts of sterols, long-chain aliphatic alcohols, phenolic acids, and acylglycerols. The ethanol-water bark extract had a high phenolic content: total phenolics ranged 211–551 mg gallic acid equivalents (GAE) g-1 of extract, tannins 76–184 mg catechin equivalents (CE) g-1 extract, and flavonoids 98–234 mg CE g-1 of extract. The antioxidant activity corresponds to 338 mg Trolox g-1 of extract. Development of high-value products is proposed through an integrated biorefinery approach including valorisation of extractives and targeting for cellulose-based applications.

scholarly journals Extraction and Characterization of Natural Dye from Green Walnut Shells and Its Use in Dyeing Polyamide: Focus on Antibacterial Properties

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mohammad Mirjalili ◽  
Loghman Karimi
Keyword(s):  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Natural Dye ◽  
Cupric Sulfate ◽  
Walnut Shells ◽  
Soxhlet Apparatus ◽  
Dyed Fabrics ◽  
Potassium Aluminum Sulfate ◽  
Layer Chromatography

Extraction of dyes from walnut using Soxhlet apparatus has been studied. The color components extracted and isolated from walnut shells were characterized by column chromatography, thin layer chromatography (TLC), nuclear magnetic resonance (NMR), mass spectroscopy (MS), and infrared (IR) techniques. Natural dye extract obtained from the walnut was used in dyeing polyamide fabrics with different mordants. The dyed fabrics were evaluated for antibacterial activity against pathogenic strains of Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. As such, the relationship between antibacterial activity and dye concentration is investigated. Durability of antibacterial activity to laundering is also discussed. Results indicate that the polyamide dyed with walnut displayed excellent antibacterial activity in the presence of ferric sulfate, cupric sulfate, and potassium aluminum sulfate and exhibited good and durable fastness properties.

scholarly journals CHARACTERIZATION OF ANTIFUNGAL ACTIVITY OF THE VARIOUS ACTIVE CONSTITUENTS OF COLEUS

2015 ◽  
Vol 2 (1) ◽  
pp. 125-127
Author(s):  
Ranjith Santhosh Kumar D.S ◽  
Lakshman Kumar B
Keyword(s):  
Methanolic Extract ◽  
Pharmaceutical Preparations ◽  
The Other ◽  
Coleus Forskohlii ◽  
Volatile Oils ◽  
Antifungal Effect ◽  
Active Constituents ◽  
Anti Fungal ◽  
Layer Chromatography

The aim of this study was to evaluate the anti-fungal effect of different solvent extracts of Coleus forskohlii which contains minor diterpenoids, deacetylforskohlin, 9-deoxyforskohlin, 1,9-deoxyforskohlin, 1,9-dideoxy-7-deacetylforskohlin, and four other diterpenoids, along with alkaloids and volatile oils. The solvent extracts included both polarized and non-polarized solvents like methanol, ethylacetate, benzene, chloroform, water, hexane, acetone and ethanol. As a result of this study, the antifungal effect was best observed with the methanolic extract against Candida albicans in comparison to the other extracts. This study also revealed that apart from forskohlin, which was confirmed by Thin Layer Chromatography, other compounds that were extracted with methanol are required to produce the antifungal effect and can be taken forward to pharmaceutical preparations.

scholarly journals Isolation and chemical characterization of saponins from lucerne seeds (Medicago media Pers.)

2015 ◽  
Vol 42 (2) ◽  
pp. 201-207 ◽  
Author(s):  
Marian Jurzysta
Keyword(s):  
Thin Layer ◽  
Red Blood Cells ◽  
Thin Layer Chromatography ◽  
Blood Cells ◽  
Glucuronic Acid ◽  
Chemical Characterization ◽  
Acid Hydrolysate ◽  
Medicagenic Acid ◽  
Layer Chromatography

Saponins of lucerne seeds have been prepared by two different methods. It was found by thin-layer chromatography that these glycosides consist of at least four components with one dominant. Soyasapogenols B, C and E were identified in acid hydrolysate of these saponins and in carbohydrate moiety glucose, galactose, rhamnose, small quantity of arabinose and xylose and glucuronic acid. It was established that saponins of lucerne seeds do not haemolyse red blood cells, contrary to top, root and blossom saponins. That may be due to the lack of medicagenic acid glycosides.

scholarly journals Development and physico-chemical characterization of Polyvinylidene fluoride (PVDF) flat sheet membranes with antibacterial properties against E. coli and S. aureus

2020 ◽  
Vol 1593 ◽  
pp. 012042
Author(s):  
Jose Gabriel Escorial ◽  
Stephen Paolo Lanzanas ◽  
Kirstine Joy Berjamin ◽  
Marielle Hannah Caliolio ◽  
Ericson Dimaunahan ◽  
...  
Keyword(s):  
Polyvinylidene Fluoride ◽  
Antibacterial Properties ◽  
Chemical Characterization ◽  
E Coli ◽  
Flat Sheet ◽  
Physico Chemical

scholarly journals STUDIES ON BIOSURFACTANT PRODUCED USING Exiguobacterium profundum

2020 ◽  
Vol 2 (2) ◽  
Author(s):  
Nur Asni Setiani ◽  
Welly Octaviyani ◽  
Syarif Hamdani ◽  
Irma Mardiah
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Antibacterial Properties ◽  
Pharmaceutical Preparations ◽  
Initial Screening ◽  
Gram Negative ◽  
Emulsification Index ◽  
Exiguobacterium Profundum ◽  
Layer Chromatography

Background: The manufacture of pharmaceutical preparations generally adds surfactants. Microbial biosurfactants can be an alternative because biodegradable and have antibacterial properties.   Objective: This study aimed to examine the biosurfactant activity of Exiguobacterium profundum.Methods: Hemolysis and spreading oil tests were performed as an initial screening. Biosurfactant production was carried out by growing bacteria on oil-enriched media with shaker system for 7 days. Biosurfactant activity can be seen from the emulsification index, while the characterization of biosurfactant were used thin layer chromatography and antibacterial qualitative testing.Results: Exiguobacterium profundum could spread the oil layer and form micelles. The emulsification index on days 0, 1, 3, 5, and 7 showed percentage in sequence 44.83%, 48.28%, 48.28%, 40%, and 43.75%. The result of TLC showed lipopeptide group which is marked with red stain with ninhydrin appearance. Antibacterial testing using Escherichia coli showed the formation of clear zones around the disk paper.Conclusion: The biosurfactant produced by Exigoubacterium profundum can be classified into lipopeptide group which has antibacterial activity against gram-negative.

Olfactory and Chemical Characterization of Indoor Air-Towards a Psychophysical Model for Air Quality

1981 ◽  
Author(s):  
Birgitta Berglund ◽  
Ulf Berglund ◽  
Thomas Lindvall ◽  
Helene Nicander-Bredberg
Keyword(s):  
Air Quality ◽  
Indoor Air ◽  
Chemical Characterization
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