scholarly journals High-Resolution UPLC-MS Profiling of Anthocyanins and Flavonols of Red Cabbage (Brassica oleracea L. var. capitata f. rubra DC.) Cultivated in Egypt and Evaluation of Their Biological Activity

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7567
Author(s):  
Khaled Ahmed Mansour ◽  
Sherifa Fahmy Moustafa ◽  
Soad Mohamed Abdelkhalik
Keyword(s):  
High Resolution ◽  
Brassica Oleracea ◽  
Methanolic Extract ◽  
Statistical Significance ◽  
Anticancer Activities ◽  
Red Cabbage ◽  
Ionization Mode ◽  
Negative Ionization ◽  
First Time ◽  
Brassica Oleracea L

In this paper, biological investigations and a high-resolution UPLC-PDA-ESI-qTOF-HRMS technique were employed for Brassica oleracea L. var. capitata f. rubra DC. (red cabbage) of the family Brassicaceae (Cruciferae), cultivated in Egypt, for the first time. The positive ionization mode is usually performed to identify anthocyanins. However, this technique cannot differentiate between anthocyanins and corresponding non-anthocyanin polyphenols. Thus, the negative ionization mode was also used, as it provided a series of characteristic ions for the MS analysis of anthocyanins. This helped in identifying five kaempferol derivatives for the first time in red cabbage, as well as nine—previously reported—anthocyanins. For the biological investigations, the acidified methanolic extract of fresh leaves and the methanolic extract of air-dried powdered leaves were examined for their antioxidant, antimicrobial, and anticancer activities. The freshly prepared phenolic extract was proven to be more biologically potent. Statistical significance was determined for its anticancer activity in comparison with standard doxorubicin.

scholarly journals Antioxidant Enzyme Activities of some Brassica Species

2017 ◽  
Vol 74 (2) ◽  
pp. 191
Author(s):  
Rodica SOARE ◽  
Maria DINU ◽  
Cristina BĂBEANU ◽  
Mihaela POPESCU
Keyword(s):  
Antioxidant Activity ◽  
Superoxide Dismutase ◽  
Enzymatic Activity ◽  
Brassica Oleracea ◽  
Radical Cation ◽  
Colorimetric Method ◽  
Antioxidant Enzyme Activities ◽  
Red Cabbage ◽  
Soluble Peroxidase ◽  
Brassica Oleracea L

This paper set out to comparatively study five species: white cabbage (Brassica oleracea L. var. capitata alba Alef.), red cabbage (Brassica oleracea L. var. capitata f. rubra Alef.), Kale (Brassica oleracea L. var. Acephala), cauliflower (Brassica oleracea var. botrytis) and broccoli (Brassica oleracea var. cymosa) in order to identify those with high enzymatic and antioxidant activities. The enzymatic activity of superoxide dismutase (SOD), catalase (CAT) and soluble peroxidase (POX) as well as the antioxidant activity against 2.2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical cation were determined. Total superoxide dismutase activity was measured spectrophotometrically based on inhibition in the photochemical reduction of nitroblue tetrazolium. Total soluble peroxidase was assayed by measuring the increase in A436 due to the guaiacol oxidation and the catalase activity was assayed through the colorimetric method. The capacity of extracts to scavenge the ABTS radical cation was assessed colorimetric using Trolox as a standard. The obtained results show that studied enzymatic activities and the antioxidant activity against ABTS vary depending on the analyzed species. So, among the studied Brassicaceae species, it emphasize red cabbage with the highest enzymatic activity (CAT 22.54 mM H2O2/min/g and POX 187.2 mM ΔA/1min/1g f.w.) and kale with highest antioxidant activity, of 767 μmol TE/100g f.w. The results of this study recommendintroducing the studied varieties in diet due to the rich antioxidant properties.

scholarly journals Kandungan Antioksidan pada Kubis Merah (Brassica oleracea L.) Dan Aplikasinya Pada Pembuatan Kerupuk

METANA ◽  
2018 ◽  
Vol 14 (1) ◽  
pp. 1
Author(s):  
Aldila Sagitaning Putri ◽  
Endang i Bekti Kristiani ◽  
Sri Haryati
Keyword(s):  
Antioxidant Activity ◽  
Brassica Oleracea ◽  
Ash Content ◽  
Agricultural Products ◽  
Anthocyanin Content ◽  
Red Cabbage ◽  
Organoleptic Test ◽  
Tapioca Flour ◽  
Brassica Oleracea L

Kubis Merah (Brassica oleracea L.) merupakan salah satu hasil pertanian yang mengandung kandungan antosianin yang berpotensi sebagai antioksidan. Tujuan yang hendak dicapai dalam penelitian ini adalah untuk menggali potensi kubis merah sebagai antioksidan alami pada kerupuk kubis merah. Tahapan dari penelitian ini adalah pembuatan kerupuk kubis merah dengan berbagai formulasi tepung tapioka dan kubis merah, uji organoleptik serta penentuan aktivitas antioksidan dan kadar antosianin. Hasil penelitian menunjukkan bahwa aktivitas antioksidan dari kubis merah dan kerupuk kubis merah sebesar. Dari uji organoleptik yang paling disukai panelis adalah perlakuan T2K1 yaitu 150 gram tapioka dan 50 gram kubis mera yang memiliki kadar air sebesar 15,49%, kadar abu 1,18%, kadar protein 1,48%, kadar lemak 16,55%, antosianin 1,31% dan aktivitas antioksidan sebesar 23,93%. Dengan demikian dapat disimpulkan bahwa kerupuk kubis merah memiliki potensi sebagai sumber antioksidan. Antioxidant content  in Red Cabbage  (Brassica oleracea L.) and Application In The Making of Crackers Red Cabbage (Brassica oleracea L.) is one of the agricultural products containing anthocyanin content that has potential as antioxidant. The purpose of  this study was to explore the potential of red cabbage as a natural antioxidant in red cabbage crackers. The stages of this research are the manufacture of red cabbage crackers with various formulations of tapioca flour and red cabbage, organoleptic test and the determination of antioxidant activity and anthocyanin levels. The results showed that antioxidant activity of red cabbage and red cabbage cracker amounted to. The most preferred organoleptic test of panelist is T2K1 treatment which is 150 g tapioca and 50 g of red cabbage which has water content 15,49%, ash content 1,18%, protein content 1,48%, fat content 16,55%, antocyanin 1.31% and antioxidant activity of 23.93%. Thus it can be concluded that red cabbage crackers have the potential as a source of antioxidants.

Effect of thermal treatment on glucosinolates and antioxidant-related parameters in red cabbage (Brassica oleracea L. ssp. capitata f. rubra)

2008 ◽  
Vol 109 (3) ◽  
pp. 595-605 ◽  
Author(s):  
Jon Volden ◽  
Grethe Iren A. Borge ◽  
Gunnar B. Bengtsson ◽  
Magnor Hansen ◽  
Ingrid E. Thygesen ◽  
...  
Keyword(s):  
Thermal Treatment ◽  
Brassica Oleracea ◽  
Red Cabbage ◽  
Brassica Oleracea L

scholarly journals NEPHROPROTECTIVE OF ANTHOCYANIN PIGMENTS EXTRACT FROM RED CABBAGE (BRASSICA OLERACEA L. VAR. CAPITATA F. RUBRA) AGAINST GENTAMICIN-CAPTOPRIL-INDUCED NEPHROTOXICITY IN RATS

2018 ◽  
Vol 11 (4) ◽  
pp. 432 ◽  
Author(s):  
Shaum Shiyan ◽  
Herlina Herlina ◽  
Lita Rizkika Sari
Keyword(s):  
Vitamin E ◽  
Citric Acid ◽  
Brassica Oleracea ◽  
Male Rats ◽  
Control Group ◽  
Anthocyanin Content ◽  
Total Anthocyanin ◽  
Red Cabbage ◽  
Nephroprotective Activity ◽  
Brassica Oleracea L

 Objective: Red cabbage (Brassica oleracea L. var. Capitata f. rubra) has a fairly high anthocyanin content and is a source of powerful antioxidants. This study is basic for the development of a nutraceutical, which has nephroprotective activity.Methods: Red cabbage was extracted using ethanol and water with a mixture of citric acid with a variation of 1, 2, and 3%. The total anthocyanin was determined with pH-differential method. The Wistar strain male rats were divided into five groups. Group 1 as a negative control, Group 2 as a positive control treated with Vitamin E dose of 400 mg/kg BW day. Groups 3–5 were treated with three different extract dose of 100, 200, and 400 mg/kg BW. Gentamicin was given intraperitoneally and captopril orally for 3 days. Extracts and Vitamin E were administrated orally for 15 days after induction of gentamicin-captopril. Nephroprotective activity was determined by measuring the levels of serum creatinine, blood ureum, and macroscopic kidney.Results: The combination of 96% ethanol and citric acid 3% showed the percent of free radical 2,2-diphenyl-1-picrylhydrazyl arrest of 75.23% and contained 53.49 ± 5.01 mg/L of total anthocyanin. The anthocyanin pigment from red cabbage extract can decrease the levels of creatinine and ureum, which dose of 100 mg/kg BW showed the highest value of 48.72%. There were differences in the macroscopic morphology in the rat kidney.Conclusion: Based on the results, we concluded that ethanol with 3% citric acid produced higher anthocyanin and showed nephroprotective activity. 

Anthocyanin-rich red cabbage (Brassica oleracea L.) extract attenuates cardiac and hepatic oxidative stress in rats fed an atherogenic diet

2012 ◽  
Vol 92 (8) ◽  
pp. 1688-1693 ◽  
Author(s):  
Jayanta M Sankhari ◽  
Menaka C Thounaojam ◽  
Ravirajsinh N Jadeja ◽  
Ranjitsinh V Devkar ◽  
A V Ramachandran
Keyword(s):  
Oxidative Stress ◽  
Brassica Oleracea ◽  
Atherogenic Diet ◽  
Red Cabbage ◽  
Hepatic Oxidative Stress ◽  
Brassica Oleracea L
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