scholarly journals A Pharmacokinetic Study of Mix-160 by LC-MS/MS: Oral Bioavailability of a Dosage Form of Citroflavonoids Mixture

Molecules ◽  
2022 ◽  
Vol 27 (2) ◽  
pp. 391
Author(s):  
Jesús Alfredo Araujo-León ◽  
Rolffy Ortiz-Andrade ◽  
Efrén Hernández-Baltazar ◽  
Emanuel Hernández-Núñez ◽  
Julio César Rivera-Leyva ◽  
...  
Keyword(s):  
Solid Phase ◽  
Drug Product ◽  
Pharmacokinetic Parameters ◽  
Intragastric Administration ◽  
Order Model ◽  
Suitable Candidate ◽  
Tablet Form ◽  
Minimal Concentration ◽  
Noncompartmental Model ◽  
Time Plot

This study was performed to evaluate and compare the pharmacokinetic parameters between two dosage formulations of hesperidin and naringenin: mixture and tablet. Our objective was to determine that the flavonoid tablet does not significantly modify the pharmacokinetic parameters compared with the mixture. For this study, we administered 161 mg/kg of either mixture (Mix-160) or tablet composed of hesperidin and by intragastric administration. Blood microsamples were collected from tail vein up to 24 h. Serum flavonoid extraction was performed by solid phase extraction and analyzed by LC-MS/MS of triple quadrupole (QqQ). Serum concentration vs. time plot showed that data fitted for a first-order model. The pharmacokinetic parameters were calculated by a noncompartmental model. The results showed that the absorption constant is higher than the elimination constant. The first concentration was found at five minutes, and minimal concentration at 24 h after administration, suggesting a enterohepatic recirculation phenomena and regulation of liver cytochromes’ activity. We did not find meaningful differences between the pharmacokinetic parameters of both samples. We concluded that tablet form did not interfere with the bioavailability of hesperidin and naringenin, and it could be a suitable candidate for developing a drug product.

Physics-based fractional-order model with simplified solid phase diffusion of lithium-ion battery

2020 ◽  
Vol 30 ◽  
pp. 101404 ◽  
Author(s):  
Dongxu Guo ◽  
Geng Yang ◽  
Xuning Feng ◽  
Xuebing Han ◽  
Languang Lu ◽  
...  
Keyword(s):  
Lithium Ion Battery ◽  
Fractional Order ◽  
Solid Phase ◽  
Lithium Ion ◽  
Order Model ◽  
Phase Diffusion ◽  
Fractional Order Model

scholarly journals Determination of Ketorolac in the Effluent from a Hospital Treating Plant and Kinetics Study of Its Photolytic Degradation

2017 ◽  
Vol 2017 ◽  
pp. 1-9
Author(s):  
Hector Hugo Ortega Soto ◽  
Jorge Javier Ramírez García ◽  
Paula Gamboa Suárez ◽  
Angie Michelle Dávila Estrada
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Degradation Kinetics ◽  
Treatment Plant ◽  
Order Model ◽  
Dark Chamber ◽  
First Order ◽  
Photolytic Degradation ◽  
Wastewater Samples

In this work, two specific, sensitive, and rapid analytical methods were developed. One of them was for the determination of ketorolac in a hospital wastewater treatment plant where there is no interference with other organic substances; the other one was for the determination of the degradation kinetics in aqueous medium. Ketorolac was extracted from wastewater samples through solid-phase extraction (SPE) cartridges, then it was identified and quantified by high-performance liquid chromatography (HPLC). Ketorolac was detected in concentrations between 0.1376 and 0.2667 μg/L. Photolytic degradation was performed on aqueous solutions of ketorolac tromethamine reference substance, at a concentration of 50 μg/mL. Samples were in direct contact with ultraviolet light in a dark chamber, equipped with two mercury lamps (254 nm) at a radiation source of 15 W. The results of the photolytic degradation were adjusted to a first-order model, obtaining a half-life of 4.8 hrs.

scholarly journals Preparation and characterization of lanolin-based condensate and its utilization as a nonionic softener for wool fabric surface

2021 ◽  
Vol 19 (5) ◽  
pp. 508-520
Author(s):  
Marwa Abou taleb ◽  
Hosam El-Sayed
Keyword(s):  
Solid Phase ◽  
Wool Fabric ◽  
Mechanical Agitation ◽  
Suitable Candidate ◽  
Poly Ethylene ◽  
Coated Fabrics ◽  
Fabric Surface ◽  
Wool Wax ◽  
Reduced Surface

Two forms of pollutants are usually discharged from scouring of wool fleece; namely the effluent liquid phase and the solid phase. These phases comprise a significant quantity of wool wax which would be a suitable candidate for valuable products and applications. This work is devoted to extraction, recovery, and characterization of lanolin from wool fleece from different sheep breeds to assign possible ways for its utilization in the textile field. The results show that the amount of wool wax extracted from coarse wool fleece as well as its chemical composition and physical properties are almost similar to those extracted from other finer wool fleece. The aim of this work is further devoted to separation and characterization of fatty acids (FAs) from the extracted wool wax. The separated wool FAs were esterified with poly ethylene glycol (PEG) to obtain a condensate which was utilized as a nonionic softener for wool. The alteration in morphology of the coated wool fabric was assessed using scanning electron microscopy. The results revealed that the WFA/PEG-coated fabrics exhibit reduced surface roughness and improved resistance to felting shrinkage during mechanical agitation.

scholarly journals Development of an UPLC–MS/MS assay to determine psoralidin in rat plasma and its application in a pharmacokinetic study after intragastric administration

2020 ◽  
Vol 32 (4) ◽  
pp. 215-218
Author(s):  
Feng Feng ◽  
Xiunan Jiang ◽  
Jieying Qiu ◽  
Hongyu Wu ◽  
Xiaojun Cai ◽  
...  
Keyword(s):  
Pharmacokinetic Study ◽  
Internal Standard ◽  
Apparent Volume ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Plasma ◽  
Volume Of Distribution ◽  
Pharmacokinetic Parameters ◽  
Intragastric Administration ◽  
Rat Tissues ◽  
Pharmacokinetic Characteristic

Psoralidin has a variety of pharmacological activities, such as anti-tumor, anti-depressant, and anti-inflammatory activities. This study aims at developing a rapid ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method to determine psoralidin in rat plasma and studying the pharmacokinetic characteristic of psoralidin after intragastric administration of 20 and 40 mg/kg. Alpinetin was used as an internal standard (IS), and the plasma samples were precipitated with acetonitrile. The calibration curves were linear over the range of 0.2–250 ng/mL (R2 = 0.993). The pharmacokinetic parameters were calculated by DAS 3.0. Half-life (t1/2) was 7.2 ± 0.97 h and 7.1 ± 0.27 h for different dosages, respectively. Tmax was 4.2 ± 1.1 h and 4.0 ± 1.1 h for different dosages, respectively. Apparent volume of distribution (Vd) for different dosages was 630.1 ± 168.8 and 600.1 ± 138.8 L/kg, respectively. Clearance (CL) was 105.6 ± 29.2 and 100.6 ± 22.2 L/h/kg for different dosages, indicating that psoralidin was mainly distributed in rat tissues. The pharmacokinetic study provided important information for further clinical application in the treatment of cancer and osteoporosis.

Comparative Pharmacokinetics of Cholecalciferol in Dogs from 2 Different Oral Formulations Using Corrective Measures to Overcome Interference from Endogenous Cholecalciferol

Drug Research ◽  
2017 ◽  
Vol 67 (07) ◽  
pp. 388-395 ◽  
Author(s):  
Harilal Patel ◽  
Prakash Patel ◽  
Chandrakant Bhatt ◽  
Ashok Ghoghari ◽  
Urvesh Patel ◽  
...  
Keyword(s):  
Solid Phase ◽  
Statistical Significance ◽  
Pharmacokinetic Analysis ◽  
Pharmacokinetic Parameters ◽  
Pharmacokinetic Studies ◽  
Serum Samples ◽  
Time Profiles ◽  
Corrective Measures ◽  
Oral Formulations ◽  
Free Serum

AbstractThe aim of the preclinical investigation was to obtain single dose pharmacokinetics in dogs from 2 different oral cholecalciferol formulations using corrective measures to overcome the interference of endogenous cholecalciferol. 6 dogs were fasted overnight and the following day received 60 000 IU dose cholecalciferol [reference, Eris®, vs. test, Sunbless®] by oral dosing. Blood samples were collected on day 0 (baseline establishment) and after dosing on day 1 up to 28 days. The serum samples were extracted using protein precipitation/solid phase extraction and analysed to determine cholecalciferol by LC-MS/MS assay with calibrators prepared from cholecalciferol free serum. Standard pharmacokinetic analysis was carried out to assess pharmacokinetic parameters. An un-paired t-test was employed for comparing statistical significance between formulations. Serum cholecalciferol concentration vs. time profiles for the 2 formulations was almost superimposable. None of the pharmacokinetic parameters showed statistically significant differences (p>0.05) between the 2 treatments. For example: Cmax (ng/mL) and AUCinf (ng.h/mL) derived after the baseline corrections were 708.65 and 38 877.18 for reference and 743.71 and 40 665.51 for test, respectively. Pharmacokinetics of cholecalciferol was comparable between reference vs. test formulations. The procedures, baseline correction and employment of cholecalciferol devoid serum, can be readily adopted in future pharmacokinetic studies in animals or humans.

Effects of Acupuncture at St36 on Pharmacokinetics of Schisandra lignans in rats

2015 ◽  
Vol 33 (3) ◽  
pp. 223-229 ◽  
Author(s):  
De Ji ◽  
Ziwan Ning ◽  
Chunqin Mao ◽  
Yong Sun ◽  
Jing Liu ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Study Groups ◽  
Acupuncture Group ◽  
Pharmacokinetic Parameters ◽  
Intragastric Administration ◽  
Schisandrin B ◽  
Time Curve ◽  
Acupuncture Stimulation ◽  
Concentration Time ◽  
Plasma Concentration Time Curve

Objective To investigate the influence of acupuncture at ST36 on the pharmacokinetics of Schisandra lignans including schisandrin, deoxyschisandrin and schisandrin B after intragastric administration of Schisandra chinensis (SC) in rats. Methods Twelve male Sprague-Dawley rats were randomly divided into two study groups: SC and SC+acupuncture. Rats in both groups received intragastric SC extract at 5.0 g/kg. Rats in the SC+acupuncture group additionally received acupuncture stimulation at ST36 for 30 min after SC administration. Acupuncture needles were rotated bilaterally for 1 min, left in situ for 20 min, then electrically stimulated for 10 min at 50 Hz frequency and 1–3 mA intensity. A sensitive and specific high performance liquid chromatography electrospray tandem mass spectrometry procedure was developed and validated for simultaneous analysis of three bioactive lignans (schisandrin, deoxyschisandrin and schisandrin B) in rat plasma. Results There were significant differences (p<0.05) between the two study groups in various pharmacokinetic parameters. Area under the plasma concentration–time curve (AUC0–t), area under the plasma concentration–time curve to time infinity (AUC0–∞) and peak plasma concentration (Cmax) for schisandrin, absorption half-life (T1/2α) and AUC0–t for deoxyschisandrin, and Cmax for schisandrin B were increased in the SC+acupuncture group compared with the SC group. T1/2α for schisandrin B only and time to peak concentration (Tmax) for all three lignans were reduced following acupuncture. Conclusions Acupuncture stimulation at ST36 affects the pharmacokinetics of SC in rats. Acupuncture may have a beneficial role in promoting the absorption of lignans from extracts of SC.

Simultaneous Determination of Three Alkaloids in Wutou Decoction in Rat Plasma Via the UPLC–MS/MS Method and its Application in Pharmacokinetic Study

2020 ◽  
Vol 16 (5) ◽  
pp. 558-563
Author(s):  
WU Jian ◽  
JI Yu-bin ◽  
Liu Ying-jie ◽  
XU Ying ◽  
Zheng Wei ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Pharmacokinetic Study ◽  
Multiple Reaction Monitoring ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Intragastric Administration ◽  
Highly Sensitive ◽  
Pharmacokinetic Properties

Introduction: Wutou decoction has been wildly applied for the treatment of RA in China for several thousand years. Methods: This study aims to develop a highly sensitive and specific ultra performance liquid chromatography coupled with tandem mass spectrometry and electrospray ionization (UPLC-ESI-MS/MS) method to explore the pharmacokinetic properties of three representative bioactive alkaloids after intragastric administration of Wutou decoction in rats. Chromatographic separation was performed on a C18 column under the Multiple Reaction Monitoring (MRM) in the positive electrospray ionization (ESI) mode. The pharmacokinetic parameters were evaluated by software DAS 3. 0. Results: The validation of the method was achieved in accordance with the FDA guidelines. The results of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. Our PK results showed that these three compounds were quickly absorbed after the administration of Wutou decoction, and Tmax ranged from 30 min to 45 min. Conclusion: The results of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. There were also similar pharmacokinetic behaviors observed among BAC, BHA, and BMA after oral administration of WTD.

Pharmacokinetic study of liquiritin in rat serum using molecularly imprinted solid-phase extraction combined with RP-HPLC

2014 ◽  
Vol 6 (5) ◽  
pp. 1563-1568 ◽  
Author(s):  
Huang Yang ◽  
Li Kang ◽  
Shao Hui Kai ◽  
Liu Sheng Quan ◽  
Xiang Fei Jun
Keyword(s):  
Solid Phase ◽  
Rat Plasma ◽  
Clinical Applications ◽  
Reliable Data ◽  
Pharmacokinetic Parameters ◽  
Rat Serum ◽  
Molecularly Imprinted ◽  
Rp Hplc ◽  
Single Oral Administration

A new method using MI-SPE-HPLC-UV for the determination of LQR in rat plasma demonstrates its applicability in analyzing pharmacokinetic parameters of LQR after a single oral administration of CLTs, so as to provide reliable data to support the safety in clinical applications.

Sign in / Sign up

Export Citation Format

Share Document