scholarly journals Integrated Graphene Oxide with Noble Metal Nanoparticles to Develop High-Sensitivity Fiber Optic Particle Plasmon Resonance (FOPPR) Biosensor for Biomolecules Determination

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 635
Author(s):  
Chien-Hsing Chen ◽  
Chang-Yue Chiang ◽  
Chin-Wei Wu ◽  
Chien-Tsung Wang ◽  
Lai-Kwan Chau
Keyword(s):  
Graphene Oxide ◽  
Linear Relationship ◽  
Plasmon Resonance ◽  
Fiber Optic ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Surface Concentration ◽  
Analytical Performance ◽  
Noble Metal Nanoparticles ◽  
Particle Plasmon

In this research, a direct, simple and ultrasensitive fiber optic particle plasmon resonance (FOPPR) biosensing platform for immunoglobulin G (IgG) detection was developed using a gold nanoparticle/graphene oxide (AuNP/GO) composite as signal amplification element. To obtain the best analytical performance of the sensor, experimental parameters including the surface concentration of GO on the AuNPs, formation time of the GO, the concentration of the anti-IgG and incubation time of anti-IgG were optimized. The calibration plots displayed a good linear relationship between the sensor response (ΔI/I0) and the logarithm of the analyte concentrations over a linear range from 1.0 × 10−10 to 1.0 × 10−6 g/mL of IgG under the optimum conditions. A limit of detection (LOD) of 0.038 ng/mL for IgG was calculated from the standard calibration curve. The plot has a linear relationship (correlation coefficient, R = 0.9990). The analytical performance of present work’s biosensor was better than that of our previously reported mixed self-assembled monolayer of 11-mercaptoundecanoic acid/6-mercapto-1-hexanol (MUA/MCH = 1:4) method by about three orders of magnitude. The achieved good sensitivity may be attributed to the synergistic effect between GO and AuNPs in this study. In addition, GO could immobilize more antibodies due to the abundant carboxylic groups on its surface. Furthermore, we also demonstrated that the results from this sensor have good reproducibility, with coefficients of variation (CVs) < 8% for IgG. Therefore, the present strategy provides a novel and convenient method for chemical and biochemical quantification and determination.

scholarly journals Development of the UV Spectrophotometric Method of Azithromycin in API and Stress Degradation Studies

2016 ◽  
Vol 68 ◽  
pp. 48-53 ◽  
Author(s):  
Sandip Bhimani ◽  
Gaurav Sanghvi ◽  
Trupesh Pethani ◽  
Gaurav Dave ◽  
Vishal Airao ◽  
...  
Keyword(s):  
Linear Relationship ◽  
Spectrophotometric Method ◽  
Phosphate Buffer ◽  
Macrolide Antibiotic ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Limit Of Quantification ◽  
Absorbance Maximum ◽  
Antibiotic Drug ◽  
Stress Degradation

Azithromycin (AZI) is a semi-synthetic macrolide antibiotic drug, effective against a wide variety of bacteria. The present study describes a simple, accurate, reproducible and precise UV Spectrophotometric method for the estimation of AZI (pH 6.8 Phosphate buffer). The absorbance maximum (λmax) for AZI was found to be 208nm. The method reveals high sensitivity, with linearity in the 10 µg/ml to 50 µg/ml range. The lower limit of detection was found to be 1.6µg/ml and the limit of quantification was found to be 5µg/ml. All the calibration curves demonstrated a linear relationship between the absorbance and concentration, with the correlation coefficient higher than 0.99. The % recovery was found to be 99.72%. AZI was also subjected to stress degradation under different conditions recommended by the International Conference on Harmonization (ICH).

scholarly journals Greener Monolithic Solid Phase Extraction Biosorbent Based on Calcium Cross-Linked Starch Cryogel Composite Graphene Oxide Nanoparticles for Benzo(a)pyrene Analysis

Molecules ◽  
2021 ◽  
Vol 26 (20) ◽  
pp. 6163
Author(s):  
Aree Choodum ◽  
Nareumon Lamthornkit ◽  
Chanita Boonkanon ◽  
Tarawee Taweekarn ◽  
Kharittha Phatthanawiwat ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Solid Phase Extraction ◽  
Limit Of Detection ◽  
Solid Phase ◽  
High Sensitivity ◽  
Rice Flour ◽  
Oxide Nanoparticles ◽  
Phase Extraction ◽  
Limit Of Quantification ◽  
Significant Difference

Benzo(a)pyrene (BaP) has been recognized as a marker for the detection of carcinogenic polycyclic aromatic hydrocarbons. In this work, a novel monolithic solid-phase extraction (SPE) sorbent based on graphene oxide nanoparticles (GO) in starch-based cryogel composite (GO-Cry) was successfully prepared for BaP analysis. Rice flour and tapioca starch (gel precursors) were gelatinized in limewater (cross-linker) under alkaline conditions before addition of GO (filler) that can increase the ability to extract BaP up to 2.6-fold. BaP analysis had a linear range of 10 to 1000 µgL−1 with good linearity (R2 = 0.9971) and high sensitivity (4.1 ± 0.1 a.u./(µgL−1)). The limit of detection and limit of quantification were 4.21 ± 0.06 and 14.04 ± 0.19 µgL−1, respectively, with excellent precision (0.17 to 2.45%RSD). The accuracy in terms of recovery from spiked samples was in the range of 84 to 110% with no significant difference to a C18 cartridge. GO-Cry can be reproducibly prepared with 2.8%RSD from 4 lots and can be reused at least 10 times, which not only helps reduce the analysis costs (~0.41USD per analysis), but also reduces the resultant waste to the environment.

Analytical evaluation of the new Beckman Coulter Access high sensitivity cardiac troponin I immunoassay

2017 ◽  
Vol 56 (1) ◽  
Author(s):  
Giuseppe Lippi ◽  
Anna Ferrari ◽  
Giorgio Gandini ◽  
Matteo Gelati ◽  
Claudia Lo Cascio ◽  
...  
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Cardiac Troponin I ◽  
Analytical Performance ◽  
The Novel ◽  
Reference Limit ◽  
Automated Immunoassay ◽  
Upper Reference Limit

AbstractBackground:This study was aimed to evaluate the analytical performance of the novel chemiluminescent and fully-automated Beckman Coulter Access hsTnI high-sensitivity immunoassay for measurement of cardiac troponin I (cTnI).Methods:The study, using lithium heparin samples, included assessment of limit of blank (LOB), limit of detection (LOD), functional sensitivity, linearity, imprecision (within run, between-run and total), calculation of 99th percentile upper reference limit (URL) in 175 healthy blood donors (mean age, 36±12 years; 47% women) and comparison with two other commercial cTnI immunoassays.Results:The LOB, LOD and functional sensitivity of Access hsTnI were 0.14, 0.34 and 1.35 ng/L, respectively. The within-run, between-run and total imprecision was 2.2%–2.9%, 4.6%–5.4%, and 5.4%–6.1%, respectively. The linearity was excellent in the range of cTnI values between 0.95 and 4195 ng/L (r=1.00). The 99th percentile URL was 15.8 ng/L. Measurable cTnI values were found in 173/175 healthy subjects (98.9%). Good agreement of cTnI values was found with AccuTnI+3 (r=0.97; mean bias, −9.3%), whereas less satisfactory agreement was found with Siemens Dimension Vista cTnI (r=0.95; mean bias, −55%).Conclusions:The results of our evaluation of the Beckman Coulter Access hsTnI indicate that the analytical performance of this fully-automated immunoassay is excellent.

Reduced Graphene Oxide/Multiwalled Carbon Nanotube Composite Decorated with Fe3O4 Magnetic Nanoparticles for Electrochemical Determination of Hydrazine in Environmental Water

2020 ◽  
Vol 20 (5) ◽  
pp. 3148-3156 ◽  
Author(s):  
S. Nehru ◽  
Subramanian Sakthinathan ◽  
P. Tamizhdurai ◽  
Te-Wei Chiu ◽  
K. Shanthi
Keyword(s):  
Graphene Oxide ◽  
Carbon Nanotube ◽  
Magnetic Nanoparticles ◽  
Reduced Graphene Oxide ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
High Sensitivity ◽  
Multiwalled Carbon Nanotube ◽  
Multiwalled Carbon ◽  
Reduced Graphene

In the present work, a reduced graphene oxide and multiwalled carbon nanotube (RGO/MWCNTFe3O4) composite decorated with Fe3O4 magnetic nanoparticles was prepared as an electrochemical sensor. The surface morphology of the prepared composite was identified by scanning electron microscopy and X-ray diffraction. The electrochemical properties of the GCE/RGO/MWCNT-Fe3O4 electrode were investigated by electrochemical impedance spectroscopy, cyclic voltammetry and amperometry. The GCE/RGO/MWCNT-Fe3O4 electrode exhibited higher electrocatalytic performance towards the oxidation of hydrazine. In the optimal conditions, the GCE/RGO/MWCNT-Fe3O4 electrode showed a wide linear range (0.15–220 μM), low limit of detection (LOD) (0.75 μM), and high sensitivity (2.868 μA μM−1 cm−2). The prepared GCE/RGO/MWCNT-Fe3O4 electrode also had excellent repeatability, selectivity, and reproducibility. The practical application of the electrode was confirmed with various spiked water samples and demonstrated acceptable recovery.

Fiber optic particle plasmon resonance immunosensor for rapid and sensitive detection of methamphetamine based on competitive inhibition

2020 ◽  
Vol 157 ◽  
pp. 105026 ◽  
Author(s):  
Ting-Chou Chang ◽  
Chang-Yue Chiang ◽  
Min-Hsiu Lin ◽  
I-Kuang Chen ◽  
Lai-Kwan Chau ◽  
...  
Keyword(s):  
Plasmon Resonance ◽  
Fiber Optic ◽  
Competitive Inhibition ◽  
Sensitive Detection ◽  
Particle Plasmon
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