Pressurized Liquid Extraction Combined with Enzymatic-Assisted Extraction to Obtain Bioactive Non-Extractable Polyphenols from Sweet Cherry (Prunus avium L.) Pomace

Sweet cherry generates large amounts of by-products within which pomace can be a source of bioactive phenolic compounds. Commonly, phenolic compounds have been obtained by conventional extraction methodologies. However, a significant fraction, called non-extractable polyphenols (NEPs), stays held in the conventional extraction residues. Therefore, in the present work, the release of NEPs from cherry pomace using pressurized liquid extraction (PLE) combined with enzyme-assisted extraction (EAE) using PromodTM enzyme is investigated for the first time. In order to study the influence of temperature, time, and pH on the NEPs extraction, a response surface methodology was carried out. PLE-EAE extracts displayed higher TPC (75 ± 8 mg GAE/100 g sample) as well as, PA content, and antioxidant capacity than the extracts obtained by PLE (with a TPC value of 14 ± 1 mg GAE/100 g sample) under the same extraction conditions, and those obtained by conventional methods (TPC of 8.30 ± 0.05 mg GAE/100 g sample). Thus, PLE-EAE treatment was more selective and sustainable to release NEPs from sweet cherry pomace compared with PLE without EAE treatment. Besides, size-exclusion chromatography profiles showed that PLE-EAE allowed obtaining NEPs with higher molecular weight (>8000 Da) than PLE alone.

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Combining Pressurized Liquid Extraction and Enzymatic-Assisted Extraction to Obtain Bioactive Non-Extractable Polyphenols from Sweet Cherry (Prunus avium L.) Pomace

10.20944/preprints202107.0631.v1 ◽

2021 ◽

Author(s):

Gloria Domínguez-Rodríguez ◽

María Concepción García ◽

María Luisa Marina ◽

Merichel Plaza

Keyword(s):

Antioxidant Capacity ◽

Sweet Cherry ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Size Exclusion ◽

Trolox Equivalent Antioxidant Capacity ◽

Total Phenolic ◽

Assisted Extraction ◽

Conventional Extraction ◽

Cherry Pomace

Sweet cherry pomace is a by-product that can be a source of bioactive phenolic compounds. Usually, polyphenols have been extracted using conventional extraction methodologies. However, a significant fraction, called non-extractable polyphenols (NEPs), remains retained in the conventional extraction residues. Therefore, this work is aimed, for the first time, to investigate the release of NEPs from cherry pomace combining pressurized liquid extraction (PLE) and enzyme-assisted extraction (EAE) using Promod enzyme. A response surface methodology was employed to study the influence of temperature, time, and pH on the NEPs extraction. The response variables were the total phenolic content (TPC) measured by Folin-Ciocalteu method, total proanthocyanidin (PA) content evaluated by vanillin, DMAC, and butanol/HCl assays, and total antioxidant capacity determined by Trolox equivalent antioxidant capacity and inhibition of hydroxyl radical assays. The results indicated that PLE-EAE was more suitable and selective to obtain NEPs from sweet cherry pomace than PLE alone. In fact, the extracts obtained by PLE-EAE displayed higher TPC, PA content, and bioactivity than the extracts obtained by PLE under the same extraction conditions, and those obtained by conventional methods. Moreover, size-exclusion chromatography profiles showed that the combination of PLE and EAE enabled the recovery of NEPs with higher molecular weight than PLE without EAE treatment.

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Optimization of microwave-assisted extraction and pressurized liquid extraction of phenolic compounds fromMoringa oleiferaleaves by multiresponse surface methodology

Electrophoresis ◽

10.1002/elps.201600071 ◽

2016 ◽

Vol 37 (13) ◽

pp. 1938-1946 ◽

Cited By ~ 32

Author(s):

Celia Rodríguez-Pérez ◽

Bienvenida Gilbert-López ◽

Jose Antonio Mendiola ◽

Rosa Quirantes-Piné ◽

Antonio Segura-Carretero ◽

...

Keyword(s):

Phenolic Compounds ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction

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Microwave Assisted Extraction and Pressurized Liquid Extraction of Sulfated Polysaccharides from Fucus virsoides and Cystoseira barbata

Foods ◽

10.3390/foods10071481 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1481

Author(s):

Ana Dobrinčić ◽

Sandra Pedisić ◽

Zoran Zorić ◽

Mladenka Jurin ◽

Marin Roje ◽

...

Keyword(s):

Antioxidant Activity ◽

Chemical Composition ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Sulfate Group ◽

Sulfated Polysaccharides ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Cystoseira Barbata

Sulfated polysaccharide fucoidan isolated from brown algae shows a wide range of biological activities that are significantly dependent on its chemical composition, which is closely related to the applied technique and extraction parameters. Therefore, the objective of this study was to evaluate the influence of microwave assisted extraction (MAE) and pressurized liquid extraction (PLE) parameters (solvent, temperature, time, and number of cycles) on the Fucus virsoides and Cystoseira barbata polysaccharide yield (%PS) and chemical composition (total sugar, fucose, and sulfate group). The optimal MAE parameters that resulted in the highest polysaccharide extraction from F. virsoides and C. barbata were 0.1 M H2SO4 for 10 min at 80 °C, while the optimal PLE parameters were 0.1 M H2SO4, for two cycles of 15 min at 140 °C. Furthermore, the %PS, chemical structure, molecular properties, and antioxidant activity of the F. virsoides and C. barbata polysaccharide extracts obtained with MAE, PLE, and conventional extraction (CE) performed under previously determinate optimal conditions were compared. PLE resulted in a significantly higher %PS from F. virsoides, while for C. barbata, a similar yield was achieved with CE and PLE, as well as CE and MAE, for both algae. Furthermore, the polysaccharides obtained using PLE had the highest polydispersity index, fucose, and sulfate group content, and the lowest uronic acid content; however their antioxidant activity was lower.

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Microwave Assisted Extraction of Phenolic Compounds from Sour Cherry Pomace

Separation Science and Technology ◽

10.1080/01496395.2011.644616 ◽

2012 ◽

Vol 47 (8) ◽

pp. 1248-1254 ◽

Cited By ~ 39

Author(s):

Meric Simsek ◽

Gulum Sumnu ◽

Serpil Sahin

Keyword(s):

Phenolic Compounds ◽

Sour Cherry ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Cherry Pomace

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Comparison of pressurized liquid extraction and microwave assisted extraction for the determination of organochlorine pesticides in vegetables

Talanta ◽

10.1016/j.talanta.2006.07.012 ◽

2007 ◽

Vol 71 (3) ◽

pp. 1345-1351 ◽

Cited By ~ 65

Author(s):

M. Barriada-Pereira ◽

M.J. González-Castro ◽

S. Muniategui-Lorenzo ◽

P. López-Mahía ◽

D. Prada-Rodríguez ◽

...

Keyword(s):

Organochlorine Pesticides ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction

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Antioxidant Activity and Phenolic Content of Campomanesia Phaea Extracts Obtained by Pressurized Liquid Extraction

The Open Food Science Journal ◽

10.2174/1874256401911010056 ◽

2019 ◽

Vol 11 (1) ◽

pp. 56-65

Author(s):

Daniele Martins Firmiano ◽

Gabriela Marques Bittencourt ◽

Alessandra Lopes de Oliveira

Keyword(s):

Antioxidant Activity ◽

Particle Size ◽

Phenolic Compounds ◽

Phenolic Content ◽

Fixed Bed ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Total Phenolic ◽

High Antioxidant Activity ◽

The Mean

Objective: This research used the Pressurized Liquid Extraction process (PLE) to obtain cambuci extracts rich in phenolic compounds and, consequently, with high antioxidant activity. Methods: We determined the centesimal composition and the mean particle size of the dried and crushed sample for characterization of the fixed bed extractor. Extractions with pressurized ethanol were performed following a Central Composite Design (CCD) with temperature (60 °C at the center point with ± 10 °C amplitude) and the contact time between the solvent and the matrix (6 min at the center point with ± 2 min amplitude) as independent variables in the process. The Total Phenolic Content (TPC) of compounds and antioxidant activity were determined. Results: The mean particle size of the cambuci in the PLE was 263.4 μm with apparent and real densities of 32.42 g/cm3 and 1.62 g/cm3, respectively, resulting in a fixed bed porosity of 0.43. The centesimal composition of the comminuted and dried sample was 12.5 ± 0.1% moisture, 3.2 ± 0.2% ash, 3.35 ± 0.07% crude protein, 10.32 ± 0.08% crude fiber and 0.35 ± 0.04% ethereal extract. The highest extraction yield (41.7%) was obtained by using the binomial 70 ºC with 8 min of solvent contact in three batches. Extracts with the highest TPC (6501.10 mg AGE/100 g sample) were obtained by the binomial 74 °C with 6 min of contact; the binomial 50 °C with 4 min of contact gave rise to the extract with the highest antioxidant activity. The CCD showed high yield extracts with high antioxidant activity and a high total phenolic compound content. Conclusion: With the use of the proposed experimental design, it was possible to optimize the extraction of total phenolic compounds from the sample.

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Ultrasonically-Assisted and Conventional Extraction from Erodium Glaucophyllum Roots Using Ethanol:Water Mixtures: Phenolic Characterization, Antioxidant, and Anti-Inflammatory Activities

Molecules ◽

10.3390/molecules25071759 ◽

2020 ◽

Vol 25 (7) ◽

pp. 1759 ◽

Cited By ~ 2

Author(s):

Francisco J. Barba ◽

Cristina Alcántara ◽

Radhia Abdelkebir ◽

Christine Bäuerl ◽

Gaspar Pérez-Martínez ◽

...

Keyword(s):

Phenolic Compounds ◽

Necrosis Factor Alpha ◽

Assisted Extraction ◽

Potent Inhibition ◽

Tnf Α ◽

Ultrasonic Assisted ◽

Trolox Equivalent ◽

Factor Alpha ◽

Conventional Extraction ◽

Anti Inflammatory

The paper presents experimental results concerning the ultrasonically-assisted extraction of bioactive compounds from Erodium glaucophyllum roots. A comparison with conventional methodology is presented, and thereby the phytochemical composition and the antioxidant and anti-inflammatory activities of extracts are evaluated. The phenolic profile of Erodium extracts was analyzed by TOF–LC–MS–MS. The identification of phenolic compounds revealed that the major component was (+)-gallocatechin in the aqueous extracts obtained for the different extraction methodologies. The highest quantity of phenolic compounds and antioxidant capacity was found in the hydroethanolic extract obtained by conventional extraction (29.22–25.50 mg GAE/g DM; 21.174 mM Trolox equivalent). The highest content of carotenoids, varying from 0.035 to 0.114 mg/g dry matter, was reached by ultrasonic-assisted extraction. Furthermore, Erodium extracts showed a potent inhibition of the inflammatory reaction by means of the inhibition of tumor necrosis factor-alpha (TNF-α). The extracts obtained when ultrasound extraction was combined with ethanol:water (50:50, v/v) presented the greatest inhibition (92%).

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Pressurized Liquid Extraction and Microwave-Assisted Extraction in the Determination of Organochlorine Pesticides in Fish Muscle Samples

Journal of AOAC International ◽

10.1093/jaoac/91.1.174 ◽

2008 ◽

Vol 91 (1) ◽

pp. 174-180 ◽

Cited By ~ 1

Author(s):

Mercedes Barriada-Pereira ◽

Iván Iglesias-García ◽

María J Gonzlez-Castro ◽

Soledad Muniategui-Lorenzo ◽

Purificación López-Maha ◽

...

Keyword(s):

Organochlorine Pesticides ◽

Fish Muscle ◽

Extraction Methods ◽

Liquid Extraction ◽

Pressurized Liquid Extraction ◽

Microwave Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Relative Standard

Abstract This paper describes a comparative study of 2 extraction methods, pressurized liquid extraction (PLE) and microwave-assisted extraction (MAE), for the determination of organochlorine pesticides (OCPs) in fish muscle samples. In both cases, samples were extracted with hexaneacetone (50 + 50), and the extracts were purified by solid-phase extraction using a carbon cartridge as the adsorbent. Pesticides were eluted with hexaneethyl acetate (80 + 20) and determined by gas chromatography with electron-capture detection. Both methods demonstrated good linearity over the range studied (0.0050.100 g/mL). Detection limits ranged from 0.029 to 0.295 mg/kg for PLE and from 0.003 to 0.054 mg/kg for MAE. For most of the pesticides, analytical recoveries with both methods were between 80 and 120, and the relative standard deviations were <10. The proposed methods were shown to be powerful techniques for the extraction of OCPs from fish muscle samples. Although good recovery rates were obtained with both extraction methods, MAE provided advantages with regard to sample handling, cost, analysis time, and solvent consumption. Acceptable validation parameters were obtained although MAE was shown to be more sensitive than PLE.

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