scholarly journals Molecular Survey of Metastrongyloid Lungworms in Domestic Cats (Felis silvestris catus) from Romania: A Retrospective Study (2008–2011)

Pathogens ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 80 ◽  
Author(s):  
Adriana Gyӧrke ◽  
Mirabela Oana Dumitrache ◽  
Zsuzsa Kalmár ◽  
Anamaria Ioana Paştiu ◽  
Viorica Mircean
Keyword(s):  
Significant Risk ◽  
Felis Silvestris ◽  
Domestic Cats ◽  
Felis Silvestris Catus ◽  
Chain Reaction ◽  
Aelurostrongylus Abstrusus ◽  
Clinical Presentations ◽  
Faecal Samples ◽  
Polymerase Chain ◽  
Respiratory Conditions

Background: Lungworms are recognized as important agents in the pathology of the respiratory system in domestic cats. While Aelurostrongylus abstrusus is worldwide known and studied, Troglostrongylus brevior has gained the attention of the scientific community only in the last decade. The pathogenicity of this species seems to be higher than A. abstrusus, causing more severe clinical presentations and being potentially fatal, especially in young animals. Methods: In this study, 371 DNA isolates of faecal samples were tested by multiplex polymerase chain reaction for the presence of A. abstrusus, T. brevior, and Angiostrongylus chabaudi. Results: The results showed that 30.2% and 6.7% of the investigated domestic cats were positive for A. abstrusus and T. brevior respectively, stressing out the importance of these parasites as agents of respiratory conditions in domestic cats from Romania. None of the samples were positive for A. chabaudi. The age, the outdoor access, and the lack of deworming were identified as significant risk factors for infection with A. abstrusus. Conclusions: This paper represents the first report of T. brevior in domestic cats from Romania. Moreover, it presents the most northern localization in Europe of T. brevior.

scholarly journals Aelurostrongilosis en un gato (Felis silvestris catus) de la Ciudad de México

2020 ◽  
Vol 6 ◽  
Author(s):  
María De Jesús Tovar Dorantes ◽  
Tania Díaz-Hernández ◽  
Mirna Karely Larios-Barajas ◽  
Araceli Lima-Melo ◽  
Luis Núñez-Ochoa
Keyword(s):  
Mexico City ◽  
Felis Silvestris ◽  
Domestic Cats ◽  
Felis Silvestris Catus ◽  
Aelurostrongylus Abstrusus ◽  
Baermann Technique ◽  
Pyrantel Pamoate ◽  
First Case ◽  
Lavage Sample ◽  
Abstract Case

Clínica Veterinaria: abordaje diagnóstico y terapéutico ISSN: 2395-8766Una forma de citar este artículo:Tovar-Dorantes M de J, Díaz-Hernández T, Larios-Barajas MK, Lima-Melo A, Núñez-Ochoa L. Aelurostrongilosis en un gato (Felis silvestris catus) de la Ciudad de México. Clínica veterinaria: abordaje diagnóstico y terapéutico. 2020;6.e57202064. doi: 10.22201/fmvz.23958766e.2020.6.57Descripción del caso. Gato doméstico macho de cinco meses de edad con taquipnea, dificultad respiratoria y patrón restrictivo con un patrón intersticial no estructurado generalizado observado en el estudio radiográfico de campos pulmonares.Hallazgos clínicos. El diagnóstico se obtuvo mediante la observación de larvas de Aelurostrongylus abstrusus en el lavado traqueobronquial y se corroboró en heces por la técnica de Baermann seriada.Tratamiento y evolución. Se eligió un esquema de tratamiento con doxiciclina a 5 mg/kg vía oral cada 12 h por quince días; fenbendazol 50 mg/kg con pamoato de pirantel 20 mg/kg y prazicuantel 5 mg/kg vía oral cada 24 h por tres días consecutivos; prednisolona a 1 mg/kg vía oral cada 12 h dosis reducción por quince días; omeprazol a 1.25 mg/kg vía oral cada 12 h por diez días y pipeta tópica de imidacloprid 10 % y moxidectina 1 % al cuarto día de iniciado el tratamiento. El paciente mostró mejoría clínica.Pruebas de laboratorio. Los estudios radiográficos de los días 37 y 143 de evolución y el resultado negativo del estudio coproparasitoscópico por la técnica de Baermann seriada los días 53 y 143 de evolución corroboraron la mejora clínica; así el tratamiento fue efectivo.Relevancia clínica. Este es el segundo caso informado en México de Aelurostrongylus abstrusus en gatos domésticos y en una ciudad diferente a la del primer caso.  Abstract Case description. A 5-month-old, male, indoor-outdoor domestic shorthair cat from Mexico City with cough, tachypnea and respiratory distress. Thoracic radiographs revealed a generalized unstructured interstitial pattern. Diagnostic and interpretation. Diagnosis of Aelurostrongylus abstrusus was obtained by observing larvae in a tracheobronchial lavage sample and corroborated through Baermann funnel technique.Treatment and clinical response. The chosen treatment scheme was doxycycline at 5 mg/kg orally every 12 h for 15 days; fenbendazole 50 mg/kg with pyrantel pamoate 20 mg/kg and praziquantel 5 mg/kg orally every 24 h for 3 consecutive days; prednisolone at 1 mg/kg oral every 12 h reduction dose over 15 days; omeprazole at 1.25 mg/kg orally every 12 h for 10 days and topical imidacloprid 10% and moxidectin 1% pippete at fourth day of treatment. The patient had clinical improvement.Laboratory tests. The patient improvement was corroborated by the radiographic studies on days 37 and 143 of evolution and in the coproparasitoscopic study, by serial Baermann technique, negative on day 53 and 143 of evolution.Clinical relevance. This is the second case report of Aelurostrongylus abstrusus in domestic cats in Mexico, happening in a different city from the first case.

scholarly journals Aelurostrongylus abstrusus Infections in Domestic Cats (Felis silvestris catus) from Antioquia, Colombia

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 337
Author(s):  
Sara Lopez-Osorio ◽  
Jeffer Leonardo Navarro-Ruiz ◽  
Astrid Rave ◽  
Anja Taubert ◽  
Carlos Hermosilla ◽  
...  
Keyword(s):  
Small Animal ◽  
Federal State ◽  
Felis Silvestris ◽  
Domestic Cats ◽  
Felis Silvestris Catus ◽  
Aelurostrongylus Abstrusus ◽  
Stray Cats ◽  
Veterinary Surgeons ◽  
Current Survey ◽  
Baermann Funnel

Although Aelurostrongylus abstrusus infections in domestic cats (Felis silvestris catus) have sporadically been reported in Colombia, there is still no data available on epidemiology nor on the biology of this neglected lungworm parasite. Thus, this epidemiological study aimed to evaluate the occurrence of patent A. abstrusus infections in domestic cats from the Colombian Federal State of Antioquia. In total, 473 fecal samples of indoor/outdoor domestic cats were collected and analyzed thereafter by the Baermann funnel migration technique for the presence of A. abstrusus first stage larvae 1 (L1). The occurrence of A. abstrusus was confirmed in 0.4% (2/473) of investigated cats. Due to the presence of patent A. abstrusus infections in investigated cats, it is unfailing to include this lungworm within differential diagnoses of feline pulmonary disorders. Despite the fact that the Baermann funnel technique is currently the cheapest and the gold standard diagnostic tool for feline aelurostrongylosis, this technique is still unknown by Colombian veterinary surgeons and rarely utilized in small animal veterinary clinics. The current survey intends to generate awareness on this neglected parasitosis and to be considered as a baseline study for future surveys monitoring feline aelurostrongylosis not only in domestic/stray cats but also in endemic wild felid species of Colombia.

scholarly journals Acceptance of a Symptom-Based Approach for COVID-19 rtRT-PCR Testing to Conserve Resources in a Lower Middle-Income Setting

2021 ◽  
Vol 12 ◽  
pp. 215013272098771
Author(s):  
S. M. Rashed Ul Islam ◽  
Tahmina Akther ◽  
Md. Abdullah Omar Nasif ◽  
Sharmin Sultana ◽  
Saif Ullah Munshi
Keyword(s):  
Primary Method ◽  
Chain Reaction ◽  
Middle Income ◽  
Clinical Recovery ◽  
Clinical Presentations ◽  
Long Time ◽  
Nasopharyngeal Secretion ◽  
Polymerase Chain ◽  
Set Up

SARS-CoV-2 initially emerged in Wuhan, China in late 2019. It has since been recognized as a pandemic and has led to great social and economic disruption globally. The Reverse Transcriptase Real-Time Polymerase Chain Reaction (rtRT-PCR) has become the primary method for COVID-19 testing worldwide. The method requires a specialized laboratory set up. Long-term persistence of SARS-CoV-2 RNA in nasopharyngeal secretion after full clinical recovery of the patient is regularly observed nowadays. This forces the patients to spend a longer period in isolation and test repeatedly to obtain evidence of viral clearance. Repeated COVID-19 testing in asymptomatic or mildly symptomatic cases often leads to extra workload for laboratories that are already struggling with a high specimen turnover. Here, we present 5 purposively selected cases with different patterns of clinical presentations in which nasopharyngeal shedding of SARS-CoV-2 RNA was observed in patients for a long time. From these case studies, we emphasized the adoption of a symptom-based approach for discontinuing transmission-based precautions over a test-based strategy to reduce the time spent by asymptomatic and mildly symptomatic COVID-19 patients in isolation. A symptom-based approach will also help reduce laboratory burden for COVID-19 testing as well as conserve valuable resources and supplies utilized for rtRT-PCR testing in an emerging lower-middle-income setting. Most importantly, it will also make room for critically ill COVID-19 patients to visit or avail COVID-19 testing at their convenience.

scholarly journals Isolation ofMycobacterium aviumsubspeciesparatuberculosisfrom non-ruminant wildlife living in the sheds and on the pastures of Greek sheep and goats

2007 ◽  
Vol 136 (5) ◽  
pp. 644-652 ◽  
Author(s):  
M. FLOROU ◽  
L. LEONTIDES ◽  
P. KOSTOULAS ◽  
C. BILLINIS ◽  
M. SOFIA ◽  
...  
Keyword(s):  
Type Strain ◽  
Insertion Sequence ◽  
Small Ruminants ◽  
Dairy Sheep ◽  
Chain Reaction ◽  
Tissue Samples ◽  
Sheep And Goats ◽  
Faecal Samples ◽  
Polymerase Chain ◽  
Type Strains

SUMMARYThis study aimed to: (1) investigate whether non-ruminant wildlife interfacing with dairy sheep and goats of four Greek flocks endemically infected withMycobacterium aviumsubspeciesparatuberculosis(MAP) harboured MAP and (2) genetically compare the strains isolated from the wildlife to those isolated from the small ruminants of these flocks. We cultured and screened, by polymerase chain reaction (PCR), pooled-tissue samples from 327 wild animals of 11 species for the MAP-specific IS900insertion sequence. We also cultured faecal samples from 100 sheep or goats from each of the four flocks. MAP was detected in samples from 11 sheep, 12 goats, two mice, two rats, a hare and a fox. Only one rat had histopathological findings. Genetic typing categorized 21 isolates as cattle-type strains and two, from a house mouse and a goat respectively, as sheep-type strains; this is the first report of a rodent harbouring a sheep-type strain. The MAP types that were most frequently isolated amongst the sheep and goats of each flock were also the ones isolated from sympatric rodents; those isolated from the fox and hare also belonged to the predominant ruminant strains.

scholarly journals Occurrence of zoonotic Enterocytozoon bieneusi in cats in Brazil

2019 ◽  
Vol 28 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Jamille Batista Faria Prado ◽  
Carlos Alberto do Nascimento Ramos ◽  
Vagner Ricardo da Silva Fiuza ◽  
Veronica Jorge Babo Terra
Keyword(s):  
Enterocytozoon Bieneusi ◽  
Domestic Cat ◽  
Domestic Cats ◽  
Chain Reaction ◽  
Mato Grosso ◽  
Source Of Infection ◽  
Two Samples ◽  
Intestinal Pathogen ◽  
Polymerase Chain ◽  
Mato Grosso Do Sul

Abstract Enterocytozoon bieneusi is an opportunistic intestinal pathogen that infects humans and a wide variety of animals worldwide. Our aim in this study was to investigate the occurrence of E. bieneusi in a domestic cat population in Campo Grande, Mato Grosso do Sul, Brazil. Sixty fecal samples from diarrheic cats were subjected to polymerase chain reaction (PCR) and the amplicons were sequenced for identification. E. bieneusi was detected in two samples (3.3%), both identified as genotype D. This genotype has already been reported in animals and humans and is considered a zoonotic genotype. Our findings represent the first report of E. bieneusi in domestic cats in Brazil, reinforcing the importance of identifying this agent as a source of infection in animals and humans.

scholarly journals Survey of lungworm infection of domestic cats in Hungary

2019 ◽  
Vol 67 (3) ◽  
pp. 407-417 ◽  
Author(s):  
Sára Kiszely ◽  
Mónika Gyurkovszky ◽  
Norbert Solymosi ◽  
Róbert Farkas
Keyword(s):  
Clinical Signs ◽  
Morphological Identification ◽  
Domestic Cats ◽  
Aelurostrongylus Abstrusus ◽  
Respiratory Disorders ◽  
First Instar ◽  
Faecal Samples ◽  
First Time ◽  
Apparently Healthy

From 61 settlements of 12 Hungarian counties, 303 domestic cats were included in this survey. Between autumn 2016 and spring 2018, fresh faecal samples were randomly collected and examined by flotation and by the Baermann–Wetzel method for the presence of lungworm infection. No eggs of Eucoleus aerophilus were detected. Morphological identification of first instar larvae (L1) was also carried out. In the faeces of 60 cats (19.8%) from 17 settlements and Budapest, L1 of Aelurostrongylus abstrusus were found. More than half of the cats were from the western part of the country. The average number of larvae per gram of faeces was 190.2 ± 304.88. These results are in line with the former findings on the prevalence of aelurostrongylosis of domestic cats in Hungary. In addition, Oslerus rostratus was also found for the first time in the faecal samples of three cats from the eastern part of the country, infected also with Ae. abstrusus. The average age (2.51 ± 1.26 years) of infected cats indicates that lungworm infection is more common among younger cats. No relationship was found between the lung-worm infection and the sex of cats. Non-neutered cats had a significantly higher proportion of lungworm infections. Two-thirds of the infected cats were apparently healthy, and only 19 individuals showed clinical signs of respiratory disorders.

Seizures as a Consequence of Hyperviscosity Syndrome in Two Dogs Naturally Infected with Leishmania infantum

2016 ◽  
Vol 52 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Daniela Proverbio ◽  
Eva Spada ◽  
Roberta Perego ◽  
Giada Bagnagatti de Giorgi
Keyword(s):  
Monoclonal Gammopathy ◽  
Conventional Polymerase Chain Reaction ◽  
Immunofluorescence Assay ◽  
Canine Leishmaniasis ◽  
Chain Reaction ◽  
Short Term ◽  
Hyperviscosity Syndrome ◽  
Clinical Presentations ◽  
Polymerase Chain ◽  
Clinical Complaint

Serum hyperviscosity syndrome (HVS) was documented in two dogs with canine leishmaniasis (CanL) and seizures as the major clinical complaint. In both cases, laboratory abnormalities included mild non-regenerative anemia, thrombocytopenia, hypoalbuminemia, hyperproteinemia with monoclonal gammopathy, and marked serum hyperviscosity. CanL was diagnosed using cytology in one case and indirect immunofluorescence assay and conventional polymerase chain reaction in the second. Specific therapy with meglumine antimoniate and allopurinolc led to short-term remission in both dogs and normalization of serum viscosity. Although dogs rarely develop HVS, it should be suspected if hyperproteinemia and monoclonal gammopathy are present. Since CanL manifests with a variety of clinical presentations, including seizures resulting from HVS-induced central nervous system hypoxia, it should also be considered as a differential diagnosis in animals with seizures as a primary presenting sign.

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