Carp Edema Virus and Cyprinid Herpesvirus-3 Coinfection is Associated with Mass Mortality of Koi (Cyprinus carpio haematopterus) in the Republic of Korea

As koi and common carp gain importance in the Korean fish industry, the need for better diagnosis, prevention, and treatment of associated diseases has increased. In June 2019, the first known case of mass mortality involving cyprinid herpesvirus-3 (CyHV-3) and the second involving carp edema virus (CEV) occurred in a koi farm in Jeolla-do, Korea. Notably, the CEV exhibited a closer phylogenetic relationship with certain CEV strains originating from Poland, Germany, and India than with strains originating from China or Japan. Epidemiological studies and detailed surveillance and control for CEV and CyHV-3 are needed along with quarantine inspections.

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Emodin resists to Cyprinid herpesvirus 3 replication via the pathways of Nrf2/Keap1-ARE and NF-κB in the ornamental koi carp (Cyprinus carpio haematopterus)

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology ◽

10.1016/j.cbpc.2021.109023 ◽

2021 ◽

Vol 246 ◽

pp. 109023

Author(s):

Zhuoyu Wang ◽

Nan Zheng ◽

Jie Liang ◽

Qiuju Wang ◽

Xiujie Zu ◽

...

Keyword(s):

Cyprinus Carpio ◽

Koi Carp ◽

Cyprinid Herpesvirus 3 ◽

Carp Cyprinus Carpio ◽

Cyprinus Carpio Haematopterus

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Non-lethal diagnostic methods for koi herpesvirus in koi Cyprinus carpio

Diseases of Aquatic Organisms ◽

10.3354/dao03456 ◽

2020 ◽

Vol 138 ◽

pp. 195-205 ◽

Cited By ~ 1

Author(s):

E Soto ◽

E Tamez-Trevino ◽

Z Yazdi ◽

BN Stevens ◽

S Yun ◽

...

Keyword(s):

Cyprinus Carpio ◽

Carrier State ◽

Diagnostic Methods ◽

Permissive Temperature ◽

Characteristic Analysis ◽

Viral Pathogen ◽

Koi Herpesvirus ◽

Cyprinid Herpesvirus 3 ◽

Herpesvirus 1 ◽

And Control

Cyprinid herpesvirus 3, also known as koi herpesvirus (KHV), is a viral pathogen responsible for mass mortalities of carp worldwide. In this study, we compared the sensitivity and specificity of ELISA and quantitative PCR (qPCR) methods for the diagnosis of KHV in experimentally infected koi Cyprinus carpio over an 11 mo period. Koi were exposed to KHV at 18 ± 1°C (permissive temperatures for KHV disease) in laboratory-controlled conditions. At 21 d post challenge, the temperature in the system was decreased to <15°C (non-permissive temperature for KHV disease), and fish were monitored for the following 11 mo. At different time points throughout the study, samples of blood and gills were collected from exposed and control koi and subjected to qPCR and ELISA. Survival proportions of 53.3 and 98.8% in exposed and control treatments, respectively, were recorded at the end of the challenge. Traditional receiver-operating characteristic analysis was used to compare the sensitivity of the ELISA and blood and gill qPCR during permissive and non-permissive temperatures. ELISA was superior to qPCR of gills and whole-blood samples in detecting previous exposure to KHV. Similar results were obtained in a second experiment exposing koi to KHV and inducing persistent infection at >30°C (non-permissive temperature for KHV disease). Finally, KHV ELISA specificity was confirmed using cyprinid herpesvirus 1-exposed koi through a period of 3 mo. This study demonstrates that the combination of ELISA and gill qPCR should be recommended in the diagnosis of KHV exposure of suspected carrier-state fish.

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First report of carp oedema virus infection of koi (Cyprinus carpio haematopterus ) in the Republic of Korea

Transboundary and Emerging Diseases ◽

10.1111/tbed.12782 ◽

2017 ◽

Vol 65 (2) ◽

pp. 315-320 ◽

Cited By ~ 10

Author(s):

S. W. Kim ◽

J. W. Jun ◽

S. S. Giri ◽

C. Chi ◽

S. Yun ◽

...

Keyword(s):

Virus Infection ◽

Cyprinus Carpio ◽

Republic Of Korea ◽

First Report ◽

The Republic ◽

Cyprinus Carpio Haematopterus

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Modern approaches to the prevention and treatment of alterations in reproductive health in women with somatoform disorders and autonomic homeostasis

HEALTH OF WOMAN ◽

10.15574/hw.2016.116.98 ◽

2016 ◽

pp. 98-101

Author(s):

Vl.V. Podolsky ◽

◽

V.V. Podolsky ◽

Keyword(s):

Reproductive Health ◽

Hormonal Regulation ◽

Preventive Measures ◽

Somatoform Disorders ◽

Emotional Health ◽

Uterine Fibroids ◽

Reproductive Function ◽

Epidemiological Studies ◽

Prevention And Treatment ◽

Artificial Abortion

The objective: the developing of a system of preventive measures and principles of pregravid preparation for women with somatoform disorders and violation of autonomic homeostasis (VAH), in which observed changes in reproductive health (CRH) in the shape of states after undergoing artificial abortion, infertility and uterine fibroids. Patients and methods. Conducted clinical and epidemiological studies in the population of women of fertile age (WFA) allowed identifying for further examination of women with CRH in the form of state after undergoing artificial abortion, infertility and uterine fibroids in women with VAH. Further women were examined, in particular the conducted clinical and instrumental methods of research; determined the state of autonomic homeostasis and psycho emotional health of the biotopes of the organism, immunity; analyzed the hormonal regulation of the menstrual cycle; performed genetic studies and determined the morphofunctional state of reproductive system. Results. The most frequent complications during pregnancy in women who had CRH in history in the form of abortions, infertility and uterine fibroids and in the background of the PAF, there was a threat of interruption of pregnancy (often in I and II trimester – 56%) and preterm delivery (21%). The study of the catamnesis of further reproductive health found that in the case of well-conducted therapeutic measures in women undergoing artificial abortion, had infertility and uterine leiomyoma in the background of VAH, restore reproductive function, and in 82% of cases occurred a pregnancy. Conclusion. The the provided study of reproductive health, and state of various organs and systems of fertile aged women with somatoform disorders and violations of the autonomic homeostasis allowed to develop preventive measures for these women and pregravid preparation with the inclusion to the therapy Magnesium and vitamins (Magne-В6®). Key words: somatoform disorders, violation of autonomic homeostasis, changes in reproductive health, prevention and treatment, women of fertile age, Magne-В6®.

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Enrichment of common carp (Cyprinus carpio) diet with Malic acid: Effects on skin mucosal immunity, antioxidant defecne and growth performance

Annals of Animal Science ◽

10.2478/aoas-2020-0092 ◽

2020 ◽

Vol 0 (0) ◽

Author(s):

Roghieh Safari ◽

Seyed Hossein Hoseinifar ◽

Maryam Dadar ◽

Hien Van Doan

Keyword(s):

Common Carp ◽

Cyprinus Carpio ◽

Malic Acid ◽

Control Group ◽

Skin Mucus ◽

Antioxidant Genes ◽

Treatment Comparison ◽

Genes Expression ◽

Significant Difference ◽

And Control

AbstractThe present study investigated possible effects of dietary malic acid on the expression of immunity, antioxidant and growth related genes expression as well as skin mucus immune parameters in common carp. Common carp (Cyprinus carpio) fingerlings were fed diets supplemented with different levels (0 [control], 0.5%, 1%, 2%) of malic acid (MA) for 60 days. The results revealed highest expression levels of immune-related genes (tnf-alpha, il1b, il8 and lyz) in skin of common carp fed 2% MA (P < 0.05). Regarding 1% MA treatment comparison with control group, significant difference was noticed just in case of lyz (P < 0.05). Evaluation of growth related genes expression revealed no significant difference between treatments (P > 0.05). The study of antioxidant related genes (gsta and gpx) in common carp skin fed with MA, showed significant difference between treated groups and control (P < 0.05). Carps fed with 2% MA had highest alkaline phosphatase activity in skin mucus compared other treated groups and control (P < 0.05). There were no significant difference between 0.5% and 1% and control (P > 0.05). The study of total protein and total immunoglobulin (Ig) in common carp skin musus revealed no alteration following MA treatment (P > 0.05). The present data demonstrated that feeding with MA altered immune and antioxidant genes expression in skin mucus of common carp.

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Effect of Integrated Neuromuscular Exercise in Physical Education Class on Health-Related Fitness in Female Children

Healthcare ◽

10.3390/healthcare9030312 ◽

2021 ◽

Vol 9 (3) ◽

pp. 312

Author(s):

Marijana Sinđić ◽

Draženka Mačak ◽

Nikola Todorović ◽

Bianka Purda ◽

Maja Batez

Keyword(s):

Physical Education ◽

Muscular Fitness ◽

Female Children ◽

Before And After ◽

Health Related ◽

The Republic ◽

Almost All ◽

And Control ◽

Cardiorespiratory Endurance ◽

Health Related Fitness

Integrated neuromuscular training (INT) showed benefits for improving fundamental movement skills (FMS). However, the INT health-related fitness (HRF) effects are lacking. The current study aimed to determine the effects of INT implemented during physical education (PE) in a primary school in the Republic of Serbia on HRF in female children. The sample consisted of 72 healthy girls who were divided into the intervention (EG: n = 37; mean ± SD: age = 8.17 ± 0.31) and control (CG: n = 35; age = 8.11 ± 0.31) groups. The EG and CG performed the INT program and traditional PE activities two times per week within the first ~15 min of PE class, respectively. The Fitnessgram battery tests assessed the HRF (body composition, cardiorespiratory endurance, muscular fitness, and flexibility) before and after the program. After eight weeks, the EG significantly reduced all fat measures, while the CG decreased only triceps skinfold but to a smaller extent (F = 5.92, p < 0.02, ŋ2 = 0.09). Both groups significantly improved the performance of almost all muscular fitness tests (curl-ups, trunk lift, push-ups); however, the EG increased the push-ups more than the CG (F = 9.01, p < 0.01, ŋ2 = 0.14). The EG additionally improved the modified pull-ups (F = 14.09, p < 0.01, ŋ2 = 0.19) and flexed arm hang (F = 28.82, p < 0.01, ŋ2 = 0.33) tests. The flexibility and cardiorespiratory endurance of both groups did not significantly change after eight weeks. This approach of exercise showed positive acceptance and relatively good results after only eight weeks.

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Immersion immunization of koi (Cyprinus carpio) against cyprinid herpesvirus 3 (CyHV-3) with carbon nanotube-loaded DNA vaccine

Aquaculture ◽

10.1016/j.aquaculture.2021.736644 ◽

2021 ◽

Vol 539 ◽

pp. 736644

Author(s):

Feng Hu ◽

Yingying Li ◽

Qing Wang ◽

Bin Zhu ◽

Siyu Wu ◽

...

Keyword(s):

Carbon Nanotube ◽

Dna Vaccine ◽

Cyprinus Carpio ◽

Cyprinid Herpesvirus 3

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Transcriptomic analysis of koi (Cyprinus carpio) spleen tissue upon cyprinid herpesvirus 3 (CyHV3) infection using next generation sequencing

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2015.12.007 ◽

2016 ◽

Vol 49 ◽

pp. 213-224 ◽

Cited By ~ 11

Author(s):

Xuezhu Lee ◽

Yang Yi ◽

Shaoping Weng ◽

Jie Zeng ◽

Hetong Zhang ◽

...

Keyword(s):

Next Generation Sequencing ◽

Cyprinus Carpio ◽

Transcriptomic Analysis ◽

Next Generation ◽

Spleen Tissue ◽

Cyprinid Herpesvirus 3 ◽

Generation Sequencing

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The Impact of Socially Responsible Reporting on Successful Corporate Operations

Organizacija ◽

10.2478/orga-2013-0007 ◽

2013 ◽

Vol 46 (2) ◽

pp. 47-54 ◽

Cited By ~ 1

Author(s):

Anton Peršič ◽

Mirko Markič

Keyword(s):

Research Method ◽

Quantitative Research ◽

Socially Responsible ◽

The Real Estate ◽

Construction Market ◽

Responsible Conduct ◽

The Republic ◽

And Control ◽

The Impact ◽

A Company

The aim of our research was to study the impact and purpose of the reporting on socially responsible conduct on the success of corporate operations. The data and information were gathered with the quantitative research method, whereas the instrument for gathering them was a questionnaire that was distributed among 759 large and medium sized organisations from the field of market services in the Republic of Slovenia. We have established that activities aimed at socially responsible conduct are directly connected with the success of corporate operations, especially the revenue (p = 0.001), the profit of a company (p = 0.000), operational growth (p = 0.007) and operational economy (p = 0.002), and are typical for organizations with a larger number of employees (p = 0.032). In this regard, the real estate and construction market activities received the lowest scores. Research results provide theoretical as well as practical benefits for everyone dealing with the planning, implementation and control of sustainable development, as well as socially responsible conduct within the organization.

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