scholarly journals First Report on the Finding of Listeria mnocytogenes ST121 Strain in a Dolphin Brain

Pathogens ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 802
Author(s):  
Yann Sévellec ◽  
Marina Torresi ◽  
Benjamin Félix ◽  
Féderica Palma ◽  
Gabriella Centorotola ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Mediterranean Sea ◽  
Food Production ◽  
Polychlorinated Biphenyl ◽  
Foodborne Illness ◽  
Production Environment ◽  
Related Strain ◽  
Genomic Features ◽  
First Time ◽  
Production Industry

Listeria monocytogenes (Lm) is a ubiquitous bacterium that causes the foodborne illness, listeriosis. Clonal complexes (CC), such as CC121, are overrepresented in the food production industry, and are rarely reported in animals and the environment. Working within a European-wide project, we investigated the routes by which strains are transmitted from environments and animals to food and the food production environment (FPE). In this context, we report, for the first time, the occurrence of a ST121 (CC121) strain isolated from a dolphin brain. The genome was compared with the genomes of 376 CC121 strains. Genomic comparisons showed that 16 strains isolated from food were the closest to the dolphin strain. Like most of the food strains analyzed here, the dolphin strain included genomic features (transposon Tn6188, plasmid pLM6179), both described as being associated with the strain’s adaptation to the FPE. Like all 376 strains, the dolphin strain contained a truncated actA gene and inlA gene, both described as being associated with attenuated virulence. Despite this fact, the strain was able to cross blood-brain barrier in immunosuppressed dolphin exposed polychlorinated biphenyl and invaded by parasites. Our data suggest that the dolphin was infected by a food-related strain released into the Mediterranean Sea.

scholarly journals Genomic Characterization of Listeria monocytogenes Isolates Associated with Clinical Listeriosis and the Food Production Environment in Ireland

Genes ◽  
2018 ◽  
Vol 9 (3) ◽  
pp. 171 ◽  
Author(s):  
Amber Hilliard ◽  
Dara Leong ◽  
Amy O’Callaghan ◽  
Eamonn Culligan ◽  
Ciara Morgan ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Production ◽  
Production Environment ◽  
Genomic Characterization

scholarly journals Application of Whole Genome Sequencing to Aid in Deciphering the Persistence Potential of Listeria monocytogenes in Food Production Environments

2021 ◽  
Vol 9 (9) ◽  
pp. 1856
Author(s):  
Natalia Unrath ◽  
Evonne McCabe ◽  
Guerrino Macori ◽  
Séamus Fanning
Keyword(s):  
Listeria Monocytogenes ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Biofilm Formation ◽  
Food Production ◽  
Mortality Rates ◽  
Foodborne Illness ◽  
Whole Genome ◽  
Bioinformatic Tools ◽  
Production Environments

Listeria monocytogenes is the etiological agent of listeriosis, a foodborne illness associated with high hospitalizations and mortality rates. This bacterium can persist in food associated environments for years with isolates being increasingly linked to outbreaks. This review presents a discussion of genomes of Listeria monocytogenes which are commonly regarded as persisters within food production environments, as well as genes which are involved in mechanisms aiding this phenotype. Although criteria for the detection of persistence remain undefined, the advent of whole genome sequencing (WGS) and the development of bioinformatic tools have revolutionized the ability to find closely related strains. These advancements will facilitate the identification of mechanisms responsible for persistence among indistinguishable genomes. In turn, this will lead to improved assessments of the importance of biofilm formation, adaptation to stressful conditions and tolerance to sterilizers in relation to the persistence of this bacterium, all of which have been previously associated with this phenotype. Despite much research being published around the topic of persistence, more insights are required to further elucidate the nature of true persistence and its implications for public health.

scholarly journals Certain Listeria monocytogenes plasmids contribute to increased UVC ultraviolet light stress

2021 ◽  
Author(s):  
Justin M Anast ◽  
Stephan Schmitz-Esser
Keyword(s):  
Listeria Monocytogenes ◽  
Stress Response ◽  
Food Production ◽  
Uv Light ◽  
Stationary Phases ◽  
Light Stress ◽  
Resistance Mechanisms ◽  
P Value ◽  
Colony Forming Units ◽  
First Time

ABSTRACT Listeria monocytogenes is the causative agent of the highly fatal foodborne disease listeriosis and can persist in food production environments. Recent research highlights the involvement of L. monocytogenes plasmids in different stress response mechanisms, which contribute to its survival in food production facilities. Ultraviolet (UV) light in the UVC spectrum (200 to 280 nm) is used in food production to control microbial contamination. Although plasmid-encoded UV resistance mechanisms have been described in other bacteria, no research indicates that L. monocytogenes plasmids contribute to the UV stress response. The plasmids of L. monocytogenes strains 6179, 4KSM, and R479a are genetically distinct and were utilized to study the roles of plasmids in the UV response. Wildtype and plasmid-cured variant cells were grown to logarithmic or late-stationary phase, plated on agar plates, and exposed to UVC for 60 or 90 s, and colony-forming units (CFUs) were determined. CFUs of 6179 and 4KSM, bearing pLM6179 and p4KSM, respectively, were significantly (p-value < 0.05) higher than the plasmid-cured strains in both logarithmic and stationary phases. No difference in survival was observed for the R479a strain. Our data show for the first time that certain L. monocytogenes plasmids contribute to the survival of UVC light stress.

Virulence of Listeria monocytogenes Isolates from Humans and Smoked Salmon, Peeled Shrimp, and Their Processing Environments

2006 ◽  
Vol 69 (9) ◽  
pp. 2157-2160 ◽  
Author(s):  
SIGRÚN GUĐ MUNDSDÓTTIR ◽  
SYLVIE M. ROCHE ◽  
KARL G. KRISTINSSON ◽  
MÁR KRISTJÁNSSON
Keyword(s):  
Listeria Monocytogenes ◽  
Food Processing ◽  
Food Production ◽  
Raw Materials ◽  
Production Environment ◽  
Inoculation Test ◽  
Smoked Salmon ◽  
Production Environments ◽  
Highly Virulent ◽  
Subcutaneous Inoculation

The virulence of 82 Listeria monocytogenes isolates from human cases and cold-smoked salmon, cooked peeled shrimp, and their production environments was assessed using the plaque-forming assay and a subcutaneous inoculation test in mice. These isolates were previously typed using serotyping and pulsed-field gel electrophoresis. The isolates from food-production environments were collected in several surveys over the period of 5 years. Sixty-eight (99.8%) of 69 isolates tested from food and food-processing environments were considered virulent while only one was avirulent. All clinical isolates (13) were highly virulent. The isolates were from raw materials, final products, and the production environment. This stresses the importance of hygiene in the processing environment as well as among personnel to avoid contamination of the final product.

scholarly journals Salmonella serovars along two beef chains in Ethiopia

2016 ◽  
Vol 10 (11) ◽  
pp. 1168-1176 ◽  
Author(s):  
Adem Hiko ◽  
Herlinde Irsigler ◽  
Gobena Ameni ◽  
Karl-Hans Zessin ◽  
Reinhard Fries
Keyword(s):  
Food Production ◽  
Human Population ◽  
Addis Ababa ◽  
Processing Plant ◽  
Production Environment ◽  
Beef Production ◽  
Production Lines ◽  
Plant Line ◽  
Processing Line ◽  
First Time

Introduction: Salmonella has been reported from foods and the food production environment, with outbreaks occurring in the human population worldwide. Methodology: A survey on Salmonella in two beef production lines (a beef abattoir line and a processing line) in Addis Ababa, Ethiopia was conducted, with a total of 668 various samples randomly collected from animal-related materials, the environment, and a beef product (mortadella). Results: Overall, a 12.9% prevalence (26.3% from the abattoir line, 5.3% from the processing plant line) was observed. The prevalence in the abattoir line environment (36.6%) was higher than that in animal-related samples (14.7%); the reverse was true for the processing plant line. Out of 86 isolates, 10 serovars were identified, and 8 remained unidentified. The predominant serotypes were S. Saintpaul (32.5%), S. Muenchen (19.8%), and S. Larochelle (12.8%). S. Kastrup and S. London were isolated for the first time in Ethiopia. Conclusions: Data indicate open ports of entry for Salmonella, with possible transfer along the line. Further investigations from farm to fork are recommended in order to identify these positions of entry.

Evidence of a predation event on a tagged Mediterranean spearfish (Tetrapturus belone; Pisces, Istiophoridae), inferred from pop-up satellite tagging data

2020 ◽  
Vol 33 ◽  
pp. 23
Author(s):  
Danilo Malara ◽  
Pietro Battaglia ◽  
Pierpaolo Consoli ◽  
Erika Arcadi ◽  
Simonepietro Canese ◽  
...  
Keyword(s):  
Mediterranean Sea ◽  
Research Group ◽  
Marine Mammals ◽  
Biodiversity Hotspot ◽  
Pelagic Species ◽  
Archival Tag ◽  
Abiotic Parameters ◽  
Predation Event ◽  
Satellite Tagging ◽  
First Time

The Strait of Messina is located at the centre of the Mediterranean Sea and is considered a biodiversity hotspot and an obligatory seasonal passage for different pelagic species such as sharks, marine mammals, and billfishes. For the first time, in the Strait of Messina, our research group tagged a Mediterranean spearfish (Tetrapturus belone) using a pop-up satellite archival tag (PSAT). The observation of abiotic parameters (depth, light, and temperature) recorded by the PSAT confirmed that the tagged specimen was predated after about nine hours. The tag was then regurgitated 14 days after the tag deployment date. The analysis of collected data seems to indicate that the predator may be an ectothermic shark, most likely the bluntnose sixgill shark (Hexanchus griseus).

scholarly journals First Description of Two Sequence Type 2 Acinetobacter baumannii Isolates Carrying OXA-23 Carbapenemase in Pagellus acarne Fished from the Mediterranean Sea near Bejaia, Algeria

2016 ◽  
Vol 60 (4) ◽  
pp. 2513-2515 ◽  
Author(s):  
Soumia Brahmi ◽  
Abdelaziz Touati ◽  
Axelle Cadière ◽  
Nassima Djahmi ◽  
Alix Pantel ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Mediterranean Sea ◽  
Sequence Type ◽  
Content Type ◽  
Carbapenem Resistant ◽  
Selective Agar ◽  
Agar Media ◽  
The Mediterranean ◽  
First Time

ABSTRACTTo determine the occurrence of carbapenem-resistantAcinetobacter baumanniiin fish fished from the Mediterranean Sea near the Bejaia coast (Algeria), we studied 300 gills and gut samples that had been randomly and prospectively collected during 1 year. After screening on selective agar media, using PCR arrays and whole-genome sequencing, we identified for the first time two OXA-23-producingA. baumanniistrains belonging to the widespread sequence type 2 (ST2)/international clone II and harboring aminoglycoside-modifying enzymes [aac(6′)-Ib andaac(3′)-I genes].

scholarly journals Genetic relatedness among Listeria monocytogenes isolated in foods and food production chain in southern Rio Grande do Sul, Brazil

Food Control ◽  
2012 ◽  
Vol 28 (1) ◽  
pp. 171-177 ◽  
Author(s):  
Karla Sequeira Mendonça ◽  
Geovana Brenner Michael ◽  
Ana Eucares von Laer ◽  
Dulcinea Blum Menezes ◽  
Marisa Ribeiro Itapema Cardoso ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Food Production ◽  
Genetic Relatedness ◽  
Rio Grande ◽  
Rio Grande Do Sul ◽  
Production Chain

scholarly journals A Parasite of Marine Rotifers: A New Lineage of Dinokaryotic Dinoflagellates (Dinophyceae)

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Fernando Gómez ◽  
Alf Skovgaard
Keyword(s):  
Mediterranean Sea ◽  
Nw Mediterranean ◽  
Animal Hosts ◽  
Nw Mediterranean Sea ◽  
First Time ◽  
Marine Rotifers

Dinoflagellate infections have been reported for different protistan and animal hosts. We report, for the first time, the association between a dinoflagellate parasite and a rotifer host, tentativelySynchaetasp. (Rotifera), collected from the port of Valencia, NW Mediterranean Sea. The rotifer contained a sporangium with 100–200 thecate dinospores that develop synchronically through palintomic sporogenesis. This undescribed dinoflagellate forms a new and divergent fast-evolved lineage that branches among the dinokaryotic dinoflagellates.

Sign in / Sign up

Export Citation Format

Share Document