scholarly journals Particle-Size-Dependent Delivery of Antitumoral miRNA Using Targeted Mesoporous Silica Nanoparticles

Pharmaceutics ◽  
2020 ◽  
Vol 12 (6) ◽  
pp. 505 ◽  
Author(s):  
Lisa Haddick ◽  
Wei Zhang ◽  
Sören Reinhard ◽  
Karin Möller ◽  
Hanna Engelke ◽  
...  
Keyword(s):  
Charged Particle ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Particle Surface ◽  
Growth Factor Receptor ◽  
Peptide Ligand ◽  
Cellular Internalization ◽  
Release Agent ◽  
Knock Down

Multifunctional core-shell mesoporous silica nanoparticles (MSN) were tailored in size ranging from 60 to 160 nm as delivery agents for antitumoral microRNA (miRNA). The positively charged particle core with a pore diameter of about 5 nm and a stellate pore morphology allowed for an internal, protective adsorption of the fragile miRNA cargo. A negatively charged particle surface enabled the association of a deliberately designed block copolymer with the MSN shell by charge-matching, simultaneously acting as a capping as well as endosomal release agent. Furthermore, the copolymer was functionalized with the peptide ligand GE11 targeting the epidermal growth factor receptor, EGFR. These multifunctional nanoparticles showed an enhanced uptake into EGFR-overexpressing T24 bladder cancer cells through receptor-mediated cellular internalization. A luciferase gene knock-down of up to 65% and additional antitumoral effects such as a decreased cell migration as well as changes in cell cycle were observed. We demonstrate that nanoparticles with a diameter of 160 nm show the fastest cellular internalization after a very short incubation time of 45 min and produce the highest level of gene knock-down.

scholarly journals Facile Synthesis of Uniform Virus-like Mesoporous Silica Nanoparticles for Enhanced Cellular Internalization

2017 ◽  
Vol 3 (8) ◽  
pp. 839-846 ◽  
Author(s):  
Wenxing Wang ◽  
Peiyuan Wang ◽  
Xueting Tang ◽  
Ahmed A. Elzatahry ◽  
Shuwen Wang ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Cellular Internalization ◽  
Facile Synthesis

scholarly journals Biological Fate of Fe3O4 Core-Shell Mesoporous Silica Nanoparticles Depending on Particle Surface Chemistry

Nanomaterials ◽  
2017 ◽  
Vol 7 (7) ◽  
pp. 162 ◽  
Author(s):  
Estelle Rascol ◽  
Morgane Daurat ◽  
Afitz Da Silva ◽  
Marie Maynadier ◽  
Christophe Dorandeu ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Surface Chemistry ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Particle Surface ◽  
Core Shell ◽  
Biological Fate ◽  
Fe3o4 Core

scholarly journals Knock-down of ELMO1 in Paediatric Rhabdomyosarcoma Cells by Nanoparticle Mediated siRNA Delivery

10.5772/62690 ◽  
2016 ◽  
Vol 3 ◽  
pp. 4 ◽  
Author(s):  
Xinyue Huang ◽  
Helen Townley
Keyword(s):  
Cell Migration ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Sirna Delivery ◽  
Tumour Site ◽  
Knock Down ◽  
Effective Reduction

Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma that is found in children and has a poor outcome for those with metastatic disease. Two histological groups have been distinguished - embryonal (ERMS) and alveolar (ARMS) forms. The ARMS subtype has higher rates of metastasis, as well as higher levels of ELMO1, which is thought to be involved in cell migration. Therefore, the knock-down of ELMO1 by targeted siRNA could provide a mechanism to prevent the metastatic behaviour of ARMS cells. However, challenges still lie in the delivery of nucleotides to a tumour site. Herein, we have described the use of a variety of mesoporous silica nanoparticles as a delivery system for siRNA that is specific for ELMO1 and shown the effective reduction in cell invasive behaviour in these cells.

Recent Advances in Application of Azobenzenes Grafted on Mesoporous Silica Nanoparticles in Controlled Drug Delivery Systems Using Light as External Stimulus

2020 ◽  
Vol 20 (11) ◽  
pp. 1001-1016
Author(s):  
Sandra Ramírez-Rave ◽  
María Josefa Bernad-Bernad ◽  
Jesús Gracia-Mora ◽  
Anatoly K. Yatsimirsky
Keyword(s):  
Drug Delivery ◽  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Drug Delivery Systems ◽  
Mesoporous Silica Nanoparticles ◽  
High Surface ◽  
Delivery Systems ◽  
Surface Reactivity ◽  
External Stimulus ◽  
Recent Advances

Hybrid materials based on Mesoporous Silica Nanoparticles (MSN) have attracted plentiful attention due to the versatility of their chemistry, and the field of Drug Delivery Systems (DDS) is not an exception. MSN present desirable biocompatibility, high surface area values, and a well-studied surface reactivity for tailoring a vast diversity of chemical moieties. Particularly important for DDS applications is the use of external stimuli for drug release. In this context, light is an exceptional alternative due to its high degree of spatiotemporal precision and non-invasive character, and a large number of promising DDS based on photoswitchable properties of azobenzenes have been recently reported. This review covers the recent advances in design of DDS using light as an external stimulus mostly based on literature published within last years with an emphasis on usually overlooked underlying chemistry, photophysical properties, and supramolecular complexation of azobenzenes.

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