Breeding Canola (Brassica napus L.) for Protein in Feed and Food

Interest in canola (Brassica napus L.) In response to this interest, scientists have been tasked with altering and optimizing the protein production chain to ensure canola proteins are safe for consumption and economical to produce. Specifically, the role of plant breeders in developing suitable varieties with the necessary protein profiles is crucial to this interdisciplinary endeavour. In this article, we aim to provide an overarching review of the canola protein chain from the perspective of a plant breeder, spanning from the genetic regulation of seed storage proteins in the crop to advancements of novel breeding technologies and their application in improving protein quality in canola. A review on the current uses of canola meal in animal husbandry is presented to underscore potential limitations for the consumption of canola meal in mammals. General discussions on the allergenic potential of canola proteins and the regulation of novel food products are provided to highlight some of the challenges that will be encountered on the road to commercialization and general acceptance of canola protein as a dietary protein source.

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Genetic and Molecular Regulation of Seed Storage Proteins (SSPs) to Improve Protein Nutritional Value of Oilseed Rape (Brassica napus L.) Seeds

Frontiers in Plant Science ◽

10.3389/fpls.2018.00890 ◽

2018 ◽

Vol 9 ◽

Cited By ~ 15

Author(s):

Katarzyna Gacek ◽

Iwona Bartkowiak-Broda ◽

Jacqueline Batley

Keyword(s):

Brassica Napus ◽

Oilseed Rape ◽

Nutritional Value ◽

Storage Proteins ◽

Seed Storage ◽

Seed Storage Proteins ◽

Molecular Regulation ◽

Brassica Napus L

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The Use of Genetic and Gene Technologies in Shaping Modern Rapeseed Cultivars (Brassica napus L.)

Genes ◽

10.3390/genes11101161 ◽

2020 ◽

Vol 11 (10) ◽

pp. 1161

Author(s):

Linh Bao Ton ◽

Ting Xiang Neik ◽

Jacqueline Batley

Keyword(s):

Brassica Napus ◽

Abiotic Stress Tolerance ◽

Oil Quality ◽

Canola Meal ◽

Brassica Napus L ◽

Oil Crops ◽

Meal Quality ◽

Genomic Tools ◽

Gene Technologies ◽

Potential Use

Since their domestication, Brassica oilseed species have undergone progressive transformation allied with the development of breeding and molecular technologies. The canola (Brassica napus) crop has rapidly expanded globally in the last 30 years with intensive innovations in canola varieties, providing for a wider range of markets apart from the food industry. The breeding efforts of B. napus, the main source of canola oil and canola meal, have been mainly focused on improving seed yield, oil quality, and meal quality along with disease resistance, abiotic stress tolerance, and herbicide resistance. The revolution in genetics and gene technologies, including genetic mapping, molecular markers, genomic tools, and gene technology, especially gene editing tools, has allowed an understanding of the complex genetic makeup and gene functions in the major bioprocesses of the Brassicales, especially Brassica oil crops. Here, we provide an overview on the contributions of these technologies in improving the major traits of B. napus and discuss their potential use to accomplish new improvement targets.

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METHODS FOR PROTECTING SOYBEAN AND CANOLA PROTEINS FROM DEGRADATION IN THE RUMEN

Canadian Journal of Animal Science ◽

10.4141/cjas84-099 ◽

1984 ◽

Vol 64 (4) ◽

pp. 855-865 ◽

Cited By ~ 19

Author(s):

Z. MIR ◽

G. K. MacLEOD ◽

J. G. BUCHANAN-SMITH ◽

D. G. GRIEVE ◽

W. L. GROVUM

Keyword(s):

Sodium Hydroxide ◽

Protein Quality ◽

Protein Digestibility ◽

Essential Amino Acids ◽

Canola Meal ◽

Protein Ratio ◽

Amino Acid Profiles ◽

Cm Protein ◽

Canola Proteins

Soybean and canola proteins were treated with heat, formaldehyde (HCHO), sodium hydroxide (NaOH), whole fresh blood (BL) or fish hydrolysate (FH). Effect of these treatments on in situ protein degradability was measured by the nylon bag technique using fisulated steers. All treatments with the exception of heat were effective in protecting protein of soybeans and soybean meal (SBM). Canola seed protein was protected with heat at 110 °C for 2 h and with chemical treatments; all treatments were effective in protecting canola meal (CM) protein. Essential amino acid profiles of these residues from HCHO- or NaOH-treated SBM were not different from untreated SBM residue but increases in some essential amino acids were found in BL-treated SBM residue. Rat bioassays of residues from nylon bag studies revealed that protein quality of SBM, as indicated by net protein ratio, was significantly reduced (P < 0.05) by addition of HCHO, NaOH, BL or FH. Protein digestibility of SBM and CM was decreased by HCHO treatment but not by other treatments. NaOH treatment of CM had no effect on protein quality but HCHO, BL or FH treatments significantly improved (P < 0.05) protein quality of CM residue compared with untreated CM residue. These studies demonstrate that soybean and canola proteins can be effectively protected from degradation in the rumen by NaOH, BL or FH treatment without adverse effect on protein digestibility. Key words: Formaldehyde, sodium hydroxide, blood, fish hydrolysate, soybean, canola

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