scholarly journals Gene Expression in Cucurbita spp. Root and Crown during Phytophthora capsici Infection

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2718
Author(s):  
Alejandro Ayala-Doñas ◽  
Pedro Gómez ◽  
Miguel de Cara-García
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Cucurbita Pepo ◽  
Phytophthora Capsici ◽  
The Other ◽  
Post Inoculation ◽  
Basal Expression ◽  
Pathogenesis Related ◽  
Plant Pathogenesis ◽  
Related Proteins

Phytophtora capsici causes major diseases in cucurbit crops worldwide. In this study, we inoculated this pathogen into Cucurbita pepo subsp. pepo susceptible MUCU-16 and C. moschata tolerant M63. The gene expression of plant pathogenesis-related proteins chitinase (CpChiIV), lignin-forming peroxidase (CpLPOX), and defensin (CpDEF) and hormone-related enzymes salicylic acid (CpPAL) and ethylene (CpACO) was analyzed for two weeks post-inoculation in root and crown tissues. Differentially expressed genes were found between genotypes, tissues, days post-inoculation, and inoculated/non-inoculated samples. After inoculation, CpPAL and CpChiIV (crown) were downregulated in MUCU-16, while CpLPOX and CpDEF were upregulated in M63. In inoculated samples, higher expression changes were presented on days 10–14 than on day 3 for CpACO, CpLPOX, and CpDEF genes. Overexpression was higher for CpDEF compared to the other tested genes, indicating good suitability as a marker of biotic stress. The overexpression of CpDEF was higher in crown than in roots for both inoculated genotypes. The basal expression of CpPAL and CpDEF was higher in MUCU-16, but after inoculation, CpPAL and CpDEF gene expression were higher in M63. These changes suggest an association between CpDEF upregulation and tolerance, and between CpPAL downregulation and susceptibility.

scholarly journals Induce the plant resistance to pathogen infection

2014 ◽  
Vol 20 (1-2) ◽  
Author(s):  
A. Ezzat ◽  
Z. Szabó ◽  
J. Nyéki
Keyword(s):  
Gene Expression ◽  
Salicylic Acid ◽  
Systemic Acquired Resistance ◽  
Induced Defense ◽  
Acquired Resistance ◽  
Vital Role ◽  
Signal Molecule ◽  
Pathogenesis Related ◽  
Related Proteins ◽  
Direct Toxicity

Systemic acquired resistance (SAR) is a mechanism of induced defense that confers long-lasting protection against a broad spectrum of microorganisms. Salicylic acid (SA) is the signal molecule which is required for induce SAR and is associated with accumulation of pathogenesis-related proteins, which are thought to contribute to resistance. SA paly vital role in some related resistance gene expression in plant cell which have direct or indirect effect on pathogen growth as SA has direct toxicity for pathogen and in the same time has stimulation effect for some enzyme related to reduce the oxidative burst.

scholarly journals Patterns of Gene Expression Upon Infection of Soybean Plants by Phytophthora sojae

2004 ◽  
Vol 17 (10) ◽  
pp. 1051-1062 ◽  
Author(s):  
Pat Moy ◽  
Dinah Qutob ◽  
B. Patrick Chapman ◽  
Ian Atkinson ◽  
Mark Gijzen
Keyword(s):  
Gene Expression ◽  
Time Course ◽  
Phytophthora Sojae ◽  
Gene Microarray ◽  
Pathogenesis Related ◽  
Soybean Plants ◽  
Related Proteins ◽  
Phytoalexin Biosynthesis ◽  
Host Tissues ◽  
Mixed Samples

To investigate patterns of gene expression in soybean (Glycine max) and Phytophthora sojae during an infection time course, we constructed a 4,896-gene microarray of host and pathogen cDNA transcripts. Analysis of rRNA from soybean and P. sojae was used to estimate the ratio of host and pathogen RNA present in mixed samples. Large changes in this ratio occurred between 12 and 24 h after infection, reflecting the rapid growth and proliferation of the pathogen within host tissues. From the microarray analysis, soybean genes that were identified as strongly upregulated during infection included those encoding enzymes of phytoalexin biosynthesis and defense and pathogenesis-related proteins. Expression of these genes generally peaked at 24 h after infection. Selected lipoxygenases and peroxidases were among the most strongly downregulated soybean genes during the course of infection. The number of pathogen genes expressed during infection reached a maximum at 24 h. The results show that it is possible to use a single microarray to simultaneously probe gene expression in two interacting organisms. The patterns of gene expression we observed in soybean and P. sojae support the hypothesis that the pathogen transits from biotrophy to necrotrophy between 12 and 24 h after infection.

scholarly journals Potential Role of Photosynthesis in the Regulation of Reactive Oxygen Species and Defence Responses to Blumeria graminis f. sp. tritici in Wheat

2020 ◽  
Vol 21 (16) ◽  
pp. 5767
Author(s):  
Yuting Hu ◽  
Shengfu Zhong ◽  
Min Zhang ◽  
Yinping Liang ◽  
Guoshu Gong ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Salicylic Acid ◽  
Powdery Mildew ◽  
Reactive Oxygen ◽  
Post Inoculation ◽  
Oxygen Species ◽  
Chlorophyll Fluorescence Parameters ◽  
H2o2 Accumulation ◽  
Pathogenesis Related ◽  
Defence Responses

Photosynthesis is not only a primary generator of reactive oxygen species (ROS) but also a component of plant defence. To determine the relationships among photosynthesis, ROS, and defence responses to powdery mildew in wheat, we compared the responses of the Pm40-expressing wheat line L658 and its susceptible sister line L958 at 0, 6, 12, 24, 48, and 72 h post-inoculation (hpi) with powdery mildew via analyses of transcriptomes, cytology, antioxidant activities, photosynthesis, and chlorophyll fluorescence parameters. The results showed that H2O2 accumulation in L658 was significantly greater than that in L958 at 6 and 48 hpi, and the enzymes activity and transcripts expression of peroxidase and catalase were suppressed in L658 compared with L958. In addition, the inhibition of photosynthesis in L658 paralleled the global downregulation of photosynthesis-related genes. Furthermore, the expression of the salicylic acid-related genes non-expressor of pathogenesis related genes 1 (NPR1), pathogenesis-related 1 (PR1), and pathogenesis-related 5 (PR5) was upregulated, while the expression of jasmonic acid- and ethylene-related genes was inhibited in L658 compared with L958. In conclusion, the downregulation of photosynthesis-related genes likely led to a decline in photosynthesis, which may be combined with the inhibition of peroxidase (POD) and catalase (CAT) to generate two stages of H2O2 accumulation. The high level of H2O2, salicylic acid and PR1 and PR5 in L658 possible initiated the hypersensitive response.

scholarly journals Gene Expression Analysis of Induced Plum Pox Virus Resistance in Peach (Prunus Persica) by Almond (P. Dulcis) Grafting

2021 ◽  
Author(s):  
Manuel Rubio ◽  
Pedro Martínez-García ◽  
Nikbakht-Dehkordi Azam ◽  
Angela Prudencio ◽  
Eva Gómez ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Initiation ◽  
Prunus Persica ◽  
Initiation Factor ◽  
Induced Response ◽  
Plum Pox Virus ◽  
Sources Of Resistance ◽  
Transcription Initiation Factor ◽  
Pathogenesis Related ◽  
Related Proteins

Abstract No natural sources of resistance to Plum pox virus (PPV, sharka disease) have been identified in peach. However, previous studies have demonstrated that grafting ‘Garrigues’ almond onto ‘GF305’ peach seedlings heavily infected with PPV can progressively reduce disease symptoms and virus accumulation. Furthermore, grafting ‘Garrigues’ onto ‘GF305’ has completely prevented virus infection. This study aims to analyse the rewiring of gene expression associated with this resistance to PPV transmitted by grafting through phloem using RNA-Seq and RTqPCR analysis. A total of 18 candidate genes were differentially expressed after grafting ‘Garrigues’ almond onto healthy ‘GF305’ peach. Among the up-regulated genes, a HEN1 homolog stands out, which, together with the differential expression of RDR- and DCL2-homologs in some of the conditions assayed, suggests that the RNA silencing machinery is activated by PPV infection and can contribute to the resistance induced by ‘Garrigues’ almond. Glucan endo -1,3-Beta D-Glucosidase could be also relevant for the ‘Garrigues’-induced response, since its expression is much higher in ‘Garrigues’ than in ‘GF305’. We also discuss the potential relevance of the following in PPV infection and ‘Garrigues’-induced resistance: several pathogenesis-related proteins, No apical meristem proteins, the transcription initiation factor TFIIB, the Speckle-type POZ protein and a number of proteins involved in phytohormone signalling.

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