Polygonum multiflorum Extract Exerts Antioxidative Effects and Increases Life Span and Stress Resistance in the Model Organism Caenorhabditis elegans via DAF-16 and SIR-2.1

Extracts of the Chinese plant Polygonum multiflorum (PME) are used for medicinal purposes as well as food supplement due to anti-aging effects. Despite of the common use of these food supplements, experimental data on physiological effects of PME and its underlying molecular mechanisms in vivo are limited. We used the model organism Caenorhabditis elegans to analyze anti-aging-effects of PME in vivo (life span, lipofuscin accumulation, oxidative stress resistance, thermal stress resistance) as well as the molecular signaling pathways involved. The effects of PME were examined in wildtype animals and mutants defective in the sirtuin-homologue SIR-2.1 (VC199) and the FOXO-homologue DAF-16 (CF1038). PME possesses antioxidative effects in vivo and increases oxidative stress resistance of the nematodes. While the accumulation of lipofuscin is only slightly decreased, PME causes a significant elongation (18.6%) of mean life span. DAF-16 is essential for the reduction of thermally induced ROS accumulation, while the resistance against paraquat-induced oxidative stress is dependent on SIR-2.1. For the extension of the life span, both DAF-16 and SIR-2.1 are needed. We demonstrate that PME exerts protective effects in C. elegans via modulation of distinct intracellular pathways.

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Agrimonia procera Wallr. Extract Increases Stress Resistance and Prolongs Life Span in Caenorhabditis elegans via Transcription Factor DAF-16 (FoxO Orthologue)

Antioxidants ◽

10.3390/antiox7120192 ◽

2018 ◽

Vol 7 (12) ◽

pp. 192 ◽

Cited By ~ 2

Author(s):

Christina Saier ◽

Inge Gommlich ◽

Volker Hiemann ◽

Sabrina Baier ◽

Karoline Koch ◽

...

Keyword(s):

Transcription Factor ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Model Organism ◽

Amyloid Β ◽

Ethanol Extract

Agrimonia procera is a pharmacologically interesting plant which is proposed to protect against various diseases due to its high amount of phytochemicals, e.g., polyphenols. However, in spite of the amount of postulated health benefits, studies concerning the mechanistic effects of Agrimonia procera are limited. Using the nematode Caenorhabditis elegans, we were able to show that an ethanol extract of Agrimonia procera herba (eAE) mediates strong antioxidative effects in the nematode: Beside a strong radical-scavenging activity, eAE reduces accumulation of reactive oxygen species (ROS) accumulation and protects against paraquat-induced oxidative stress. The extract does not protect against amyloid-β-mediated toxicity, but efficiently increases the life span (up to 12.7%), as well as the resistance to thermal stress (prolongation of survival up to 22%), of this model organism. Using nematodes deficient in the forkhead box O (FoxO)-orthologue DAF-16, we were able to demonstrate that beneficial effects of eAE on stress resistance and life span were mediated via this transcription factor. We showed antioxidative, stress-reducing, and life-prolonging effects of eAE in vivo and were able to demonstrate a molecular mechanism of this extract. These results may be important for identifying further molecular targets of eAE in humans.

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Production of YP170 Vitellogenins Promotes Intestinal Senescence in Caenorhabditis elegans

The Journals of Gerontology Series A ◽

10.1093/gerona/glz067 ◽

2019 ◽

Vol 74 (8) ◽

pp. 1180-1188 ◽

Cited By ~ 2

Author(s):

Thanet Sornda ◽

Marina Ezcurra ◽

Carina Kern ◽

Evgeniy R Galimov ◽

Catherine Au ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Later Life ◽

Abundant Species ◽

Yolk Proteins ◽

Oxidative Stress Resistance ◽

Vitellogenin Synthesis ◽

Intestinal Atrophy

Abstract During aging, etiologies of senescence cause multiple pathologies, leading to morbidity and death. To understand aging requires identification of these etiologies. For example, Caenorhabditis elegans hermaphrodites consume their own intestinal biomass to support yolk production, which in later life drives intestinal atrophy and ectopic yolk deposition. Yolk proteins (YPs; vitellogenins) exist as three abundant species: YP170, derived from vit-1–vit-5; and YP115 and YP88, derived from vit-6. Here, we show that inhibiting YP170 synthesis leads to a reciprocal increase in YP115/YP88 levels and vice versa, an effect involving posttranscriptional mechanisms. Inhibiting YP170 production alone, despite increasing YP115/YP88 synthesis, reduces intestinal atrophy as much as inhibition of all YP synthesis, which increases life span. By contrast, inhibiting YP115/YP88 production alone accelerates intestinal atrophy and reduces life span, an effect that is dependent on increased YP170 production. Thus, despite copious abundance of both YP170 and YP115/YP88, only YP170 production is coupled to intestinal atrophy and shortened life span. In addition, increasing levels of YP115/YP88 but not of YP170 increases resistance to oxidative stress; thus, longevity resulting from reduced vitellogenin synthesis is not attributable to oxidative stress resistance.

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Faculty Opinions recommendation of Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726214939.793515605 ◽

2016 ◽

Author(s):

David Lombard

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Oxidative Stress Resistance

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Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans

Journal of Pharmacy and Pharmacology ◽

10.1111/jphp.12023 ◽

2013 ◽

Vol 65 (5) ◽

pp. 682-688 ◽

Cited By ~ 43

Author(s):

Wei Chen ◽

Leila Rezaizadehnajafi ◽

Michael Wink

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Oxidative Stress Resistance

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The Foodborne Strain Lactobacillus fermentum MBC2 Triggers pept-1-Dependent Pro-Longevity Effects in Caenorhabditis elegans

Microorganisms ◽

10.3390/microorganisms7020045 ◽

2019 ◽

Vol 7 (2) ◽

pp. 45 ◽

Cited By ~ 15

Author(s):

Emily Schifano ◽

Paola Zinno ◽

Barbara Guantario ◽

Marianna Roselli ◽

Sante Marcoccia ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Acid Tolerance ◽

Lactobacillus Fermentum ◽

In Vivo Model ◽

Aging Effects ◽

Pumping Rate ◽

Gut Colonization

Lactic acid bacteria (LAB) are involved in several food fermentations and many of them provide strain-specific health benefits. Herein, the probiotic potential of the foodborne strain Lactobacillus fermentum MBC2 was investigated through in vitro and in vivo approaches. Caenorhabditis elegans was used as an in vivo model to analyze pro-longevity and anti-aging effects. L. fermentum MBC2 showed a high gut colonization capability compared to E. coli OP50 (OP50) or L. rhamnosus GG (LGG). Moreover, analysis of pumping rate, lipofuscin accumulation, and body bending showed anti-aging effects in L. fermentum MBC2-fed worms. Studies on PEPT-1 mutants demonstrated that pept-1 gene was involved in the anti-aging processes mediated by this bacterial strain through DAF-16, whereas the oxidative stress protection was PEPT-1 independent. Moreover, analysis of acid tolerance, bile tolerance, and antibiotic susceptibility were evaluated. L. fermentum MBC2 exerted beneficial effects on nematode lifespan, influencing energy metabolism and oxidative stress resistance, resulted in being tolerant to acidic pH and able to adhere to Caco-2 cells. Overall, these findings provide new insight for application of this strain in the food industry as a newly isolated functional starter. Furthermore, these results will also shed light on C. elegans molecular players involved in host-microbe interactions.

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TSG (2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside) from the Chinese HerbPolygonum multiflorumIncreases Life Span and Stress Resistance ofCaenorhabditis elegans

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/124357 ◽

2015 ◽

Vol 2015 ◽

pp. 1-12 ◽

Cited By ~ 26

Author(s):

Christian Büchter ◽

Liang Zhao ◽

Susannah Havermann ◽

Sebastian Honnen ◽

Gerhard Fritz ◽

...

Keyword(s):

Life Span ◽

Stress Resistance ◽

Adult Life ◽

Model Organism ◽

Antioxidative Capacity ◽

Loss Of Function ◽

Polygonum Multiflorum ◽

Adult Life Span ◽

C Elegans ◽

Related Transcription Factor

2,3,5,4′-Tetrahydroxystilbene-2-O-β-D-glucoside (TSG) was isolated fromPolygonum multiflorum, a plant which is traditionally used as an anti-ageing drug. We have analysed ageing-related effects of TSG in the model organismC. elegansin comparison to resveratrol. TSG exerted a high antioxidative capacity both in a cell-free assay and in the nematode. The antioxidative capacity was even higher compared to resveratrol. Presumably due to its antioxidative effects, treatment with TSG decreased the juglone-mediated induction of the antioxidative enzyme SOD-3; the induction of the GST-4 by juglone was diminished slightly. TSG increased the resistance ofC. elegansagainst lethal thermal stress more prominently than resveratrol (50 μM TSG increased mean survival by 22.2%). The level of the ageing pigment lipofuscin was decreased after incubation with the compound. TSG prolongs the mean, median, and maximum adult life span ofC. elegansby 23.5%, 29.4%, and 7.2%, respectively, comparable to the effects of resveratrol. TSG-mediated extension of life span was not abolished in a DAF-16 loss-of-function mutant strain showing that this ageing-related transcription factor is not involved in the effects of TSG. Our data show that TSG possesses a potent antioxidative capacity, enhances the stress resistance, and increases the life span of the nematodeC. elegans.

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Effects of superoxide dismutase/catalase mimetics on life span and oxidative stress resistance in the housefly, Musca domestica

Free Radical Biology and Medicine ◽

10.1016/s0891-5849(02)00849-3 ◽

2002 ◽

Vol 32 (11) ◽

pp. 1229-1234 ◽

Cited By ~ 55

Author(s):

Anne-Cécile V. Bayne ◽

Rajindar S. Sohal

Keyword(s):

Oxidative Stress ◽

Superoxide Dismutase ◽

Life Span ◽

Musca Domestica ◽

Stress Resistance ◽

Oxidative Stress Resistance ◽

And Oxidative Stress

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Chlorophyll enhances oxidative stress tolerance inCaenorhabditis elegansand extends its lifespan

PeerJ ◽

10.7717/peerj.1879 ◽

2016 ◽

Vol 4 ◽

pp. e1879 ◽

Cited By ~ 22

Author(s):

Erjia Wang ◽

Michael Wink

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Secondary Metabolites ◽

Model System ◽

The Body ◽

Aging Effects ◽

Oxidative Stress Tolerance ◽

C Elegans ◽

Dependent Pathway

Green vegetables are thought to be responsible for several beneficial properties such as antioxidant, anti-mutagenic, and detoxification activities. It is not known whether these effects are due to chlorophyll which exists in large amounts in many foods or result from other secondary metabolites. In this study, we used the model systemCaenorhabditis elegansto investigate the anti-oxidative and anti-aging effects of chlorophyllin vivo. We found that chlorophyll significantly improves resistance to oxidative stress. It also enhances the lifespan ofC. elegansby up to 25% via activation of the DAF-16/FOXO-dependent pathway. The results indicate that chlorophyll is absorbed by the worms and is thus bioavailable, constituting an important prerequisite for antioxidant and longevity-promoting activities inside the body. Our study thereby supports the view that green vegetables may also be beneficial for humans.

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