The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties

Blueberries have seen an ascending production line boosted by World Health Organization (WHO) approvals for their contributions to a healthy diet and the evidence that they act against different diseases. This increase resulted in significant amounts of discarded leaves, which could be a valuable source of bioactive compounds. In the present study, ultrasound-assisted extraction technology was used to determine and compare the chemical and biological profiles of leaves from six commercial blueberry (Vaccinium corymbosum L.) varieties. Feruloylquinic acid was the major compound identified, ranging from 19.23 ± 0.18 mg/g (at the lowest level, registered in the Spartan variety) to 49.62 ± 0.41 mg/g (at the highest level, registered in the Nelson variety). Rutin was the second major compound identified, for which Toro, Nelson, and Elliot leaves registered the highest values, with 35.77 ± 0.19 mg/g, 32.50 ± 0.20 mg/g, and 31.53 ± 0.1 mg/g, respectively. Even though analogous polyphenols were detected in the six cultivars, their concentrations and amounts were different. The leaf extracts of the cultivars Toro, Elliot, and Nelson appear to be good sources of antioxidants, registering high percentage inhibitions of DPPH radicals, of 70.41%, 68.42%, and 58.69%, respectively. The blueberry leaf extracts had a strong antibacterial activity and a low antifungal capacity, and a low-to-moderate antimutagenic capacity towards Salmonella typhimurium TA98 and TA100 strains, with Toro leaf being the best candidate. All of these biological activities indicate health-related benefits, recommending them as suitable candidates for medical and pharmaceutical applications. The present paper adds significant knowledge to the field of blueberry leaves via chemical and biological profiles, supporting the ultrasound-assisted extraction technique as a useful and green method to provide alternative sources of bioactive compounds.

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Impact of ultrasound-assisted extraction and solvent composition on bioactive compounds and in vitro biological activities of thyme and rosemary

Food Research International ◽

10.1016/j.foodres.2020.109242 ◽

2020 ◽

Vol 134 ◽

pp. 109242 ◽

Cited By ~ 3

Author(s):

Paulo E.S. Munekata ◽

Cristina Alcántara ◽

Tihana Žugčić ◽

Radhia Abdelkebir ◽

María Carmen Collado ◽

...

Keyword(s):

Bioactive Compounds ◽

Biological Activities ◽

Solvent Composition ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

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ULTRASOUND-ASSISTED EXTRACTION OF TOTAL FLAVONOIDS FROM LEAVES OF SYRINGA OBLATA LINDL.

Latin American Applied Research - An international journal ◽

10.52292/j.laar.2014.430 ◽

2014 ◽

Vol 44 (2) ◽

pp. 131-135

Author(s):

Z. SHENG ◽

Y. WANG ◽

P. WAN ◽

Y. LI

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Plant Sample ◽

Solvent Ratio ◽

Total Flavonoids ◽

Extraction Temperature ◽

Ultrasound Assisted Extraction ◽

Extraction Technology ◽

Assisted Extraction ◽

Ultrasound Assisted

Leaves of Syringa Oblata Lindl. possess some important biological activities, such as antibacterial, antiviral, anti-inflammatory and antioxidant activities. These biological properties are mainly attributed to total flavonoids content. In this paper, ultrasound-assisted extraction of total flavonoids from leaves of Syringa Oblata Lindl. was studied. Effects of several experimental parameters, such as concentration of extracting solvent, ratio of liquid to material, extraction temperature, and time of sonication on extraction efficiencies of total flavonoids were evaluated. The best extraction conditions were: 1 g plant sample with 20 mL of 50% ethanol, at 60 °C for 50 min, obtaining a yield of total flavonoids of 92.00 ± 0.87 mg/g of plant. The results indicated that high amounts of total flavonoids can be extracted from leaves of Syringa Oblata Lindl. by ultrasound-assisted extraction technology.

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Optimization of Ultrasound-Assisted Extraction of Some Bioactive Compounds from Tobacco Waste

Molecules ◽

10.3390/molecules24081611 ◽

2019 ◽

Vol 24 (8) ◽

pp. 1611 ◽

Cited By ~ 9

Author(s):

Marija Banožić ◽

Ines Banjari ◽

Martina Jakovljević ◽

Drago Šubarić ◽

Srećko Tomas ◽

...

Keyword(s):

Antioxidant Activity ◽

Bioactive Compounds ◽

Raw Material ◽

Ultrasound Assisted Extraction ◽

Leaf Extracts ◽

Potential Source ◽

Assisted Extraction ◽

Different Types ◽

Ultrasound Assisted ◽

Tobacco Waste

This is the first study on ultrasound-assisted extraction (UAE) of bioactive compounds from different types of tobacco industry wastes (scrap, dust, and midrib). The obtained results were compared with starting raw material (tobacco leaves) to see the changes in bioactive compounds during tobacco processing. Results suggested that tobacco waste extracts possess antioxidant activity and considerable amounts of targeted bioactive compounds (phenolics and solanesol). The content of chlorogenic acid varied between 3.64 and 804.2 μg/mL, caffeic acid between 2.34 and 10.8 μg/mL, rutin between 11.56 and 93.7 μg/mL, and solanesol between 294.9 and 598.9 μg/mL for waste and leaf extracts, respectively. There were noticeable differences between bioactive compounds content and antioxidant activity in extracts related to applied UAE conditions and the used type of tobacco waste. Results show that optimal UAE parameters obtained by response surface methodology (RSM) were different for each type of material, so process optimization proved to be necessary. Considering that tobacco waste is mostly discarded or not effectively utilized, the results clearly show that tobacco waste could be used as a potential source of some bioactive compounds.

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Innovative Extraction Techniques for the Optimum Extraction of Phenolic Compounds from Peanut Meal and Evaluation of Their Biological Activities

Advances in Nutrition & Food Science ◽

10.33140/anfs.04.02.2 ◽

2019 ◽

Vol 4 (2) ◽

Keyword(s):

Phenolic Compounds ◽

Carcinoma Cell ◽

Biological Activities ◽

Peanut Meal ◽

Ultrasound Assisted Extraction ◽

Carcinoma Cell Lines ◽

Assisted Extraction ◽

Phenolic Extract ◽

Ultrasound Assisted ◽

Water Ratio

There is a worldwide demand for phenolic compounds (PC) because they exhibit several biological activities. This work aimed at extracting phenolic compounds from peanut meal. The methods of extraction were mainly: conventional solvent extraction (traditional methods) and ultrasound assisted extraction (recent methods) and comparing their results. Peanut meal (PM) was prepared by defatting with n-hexane, and then extracted by the two previous methods. First, the conventional solvents used were 80% methanol, ethanol, acetone, isopropanol, and distilled water. Then studied Different parameters such as meal: water ratio, also the effect of temperature and the pH on the extraction process. Second, ultrasonic assisted extractions (USAE), the parameters investigated were temperature, time and speed of sonication. Finally, all the extracts were analyzed by HPLC for their phenolic contents. Results indicated that the highest extracted PC achieved by solvents was in distilled water where 1:100, Meal: Water ratio which extracted 40 mg PC / g PM at 30& 35°C. Highest extracted PC was achieved by alkaline medium at pH 12 more than acidic and neutral medium. While (USAE) at speed 8 ultrasonication and temperature 30ᵒC, extracted 49.2mg PC /g PM. Sothe ultrasound assisted extraction exhibited great influence on the extraction of phenolic compounds from peanut meal. The ultrasonic peanut extract was examined for its antioxidant, antimicrobial and anticarcinogenic activities. The antioxidant activity of PM phenolic extract prepared by ultrasonic technique, was measured by, β-carotene, and DPPH methods, and reducing antioxidant power. Results revealed values: 84.57, 57.72 and 5960 respectively. The PM extract showed different levels of antimicrobial activity against the pathogenic bacteria used. As for the anticarcinogenic effect PM phenolic extract most effective on inhibiting colon carcinoma and lung carcinoma cell lines with IC50 = 20.7 and 20.8 µ/ml., respectively. This was followed by intestinal carcinoma and liver carcinoma cell lines with IC50= 39.6 and 40.2µ/ml.

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Effect of Ultrasound-Assisted Extraction Parameters on Total Polyphenols and Its Antioxidant Activity from Mango Residues (Mangifera indica L. var. Manililla)

Separations ◽

10.3390/separations8070094 ◽

2021 ◽

Vol 8 (7) ◽

pp. 94

Author(s):

Anahí J. Borrás-Enríquez ◽

Elizabeth Reyes-Ventura ◽

Socorro J. Villanueva-Rodríguez ◽

Lorena Moreno-Vilet

Keyword(s):

Antioxidant Activity ◽

Mangifera Indica ◽

Central Point ◽

Added Value ◽

Sonication Time ◽

Ultrasound Assisted Extraction ◽

Extraction Technology ◽

Total Polyphenols ◽

Assisted Extraction ◽

Ultrasound Assisted

Manililla is a mango variety whose residues contain bioactive compounds such as polyphenols and flavonoids, with high added value. The use of environmentally friendly extraction technology would be of great relevance; hence, this study aimed to evaluate the effect of solvent relation, sonication time and amplitude on the ultrasound-assisted extraction of total polyphenols in Manililla mango residues (peel, endocarp and kernel) and antioxidant activity. An experimental design 23 with a central point was used to evaluate the curvature behavior of the process variables. Conventional maceration was used as a control. The better conditions were obtained at the central point using 50% ethanol in water, 60% amplitude and 20 min of sonication time. We obtained values of up to 1814 mg GAE/100 g, 469 mg GAE/100 g and 672 mg GAE/100 g of total polyphenols and 1228 mg QE/100 g, 653 mg QE/100 g and 880 mg QE/100 g of total flavonoids for peel, endocarp and kernel, respectively. Mangiferin was quantified in ultrasound-assisted extraction at 150 mg/g in peel and 0.025 mg/g in the kernel, but it was not detectable in maceration. An antioxidant capacity of 87%, 14% and 83% inhibition for peel, endocarp and kernel, respectively, were obtained. Peel and kernel were the residues with higher potential as extraction material, while endocarp was not.

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Mathematical Modelling of Ultrasound-Assisted Extraction Kinetics of Bioactive Compounds from Artichoke By-Products

Foods ◽

10.3390/foods10050931 ◽

2021 ◽

Vol 10 (5) ◽

pp. 931

Author(s):

Cristina Reche ◽

Carmen Rosselló ◽

Mónica M. Umaña ◽

Valeria Eim ◽

Susana Simal

Keyword(s):

Power Density ◽

Bioactive Compounds ◽

Extraction Yield ◽

Total Phenolic ◽

Model Parameters ◽

Ultrasound Assisted Extraction ◽

Yield Curves ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Ultrasound Power

Valorization of an artichoke by-product, rich in bioactive compounds, by ultrasound-assisted extraction, is proposed. The extraction yield curves of total phenolic content (TPC) and chlorogenic acid content (CAC) in 20% ethanol (v/v) with agitation (100 rpm) and ultrasound (200 and 335 W/L) were determined at 25, 40, and 60 °C. A mathematical model considering simultaneous diffusion and convection is proposed to simulate the extraction curves and to quantify both temperature and ultrasound power density effects in terms of the model parameters variation. The effective diffusion coefficient exhibited temperature dependence (72% increase for TPC from 25 °C to 60 °C), whereas the external mass transfer coefficient and the equilibrium extraction yield depended on both temperature (72% and 90% increases for TPC from 25 to 60 °C) and ultrasound power density (26 and 51% increases for TPC from 0 (agitation) to 335 W/L). The model allowed the accurate curves simulation, the average mean relative error being 5.3 ± 2.6%. Thus, the need of considering two resistances in series to satisfactorily simulate the extraction yield curves could be related to the diffusion of the bioactive compound from inside the vegetable cells toward the intercellular volume and from there, to the liquid phase.

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Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

International Journal of Food Engineering ◽

10.1515/ijfe-2019-0191 ◽

2020 ◽

Vol 16 (1-2) ◽

Cited By ~ 2

Author(s):

Cassiano Brown da Rocha ◽

Caciano Pelayo Zapata Noreña

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Bioactive Compounds ◽

Grape Pomace ◽

Total Phenolic ◽

Microwave Assisted Extraction ◽

Ultrasound Assisted Extraction ◽

Microwave Assisted ◽

Assisted Extraction ◽

Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

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