scholarly journals Optimization of Ultrasound-Assisted Extraction of Some Bioactive Compounds from Tobacco Waste

Molecules ◽  
2019 ◽  
Vol 24 (8) ◽  
pp. 1611 ◽  
Author(s):  
Marija Banožić ◽  
Ines Banjari ◽  
Martina Jakovljević ◽  
Drago Šubarić ◽  
Srećko Tomas ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Leaf Extracts ◽  
Potential Source ◽  
Assisted Extraction ◽  
Different Types ◽  
Ultrasound Assisted ◽  
Tobacco Waste

This is the first study on ultrasound-assisted extraction (UAE) of bioactive compounds from different types of tobacco industry wastes (scrap, dust, and midrib). The obtained results were compared with starting raw material (tobacco leaves) to see the changes in bioactive compounds during tobacco processing. Results suggested that tobacco waste extracts possess antioxidant activity and considerable amounts of targeted bioactive compounds (phenolics and solanesol). The content of chlorogenic acid varied between 3.64 and 804.2 μg/mL, caffeic acid between 2.34 and 10.8 μg/mL, rutin between 11.56 and 93.7 μg/mL, and solanesol between 294.9 and 598.9 μg/mL for waste and leaf extracts, respectively. There were noticeable differences between bioactive compounds content and antioxidant activity in extracts related to applied UAE conditions and the used type of tobacco waste. Results show that optimal UAE parameters obtained by response surface methodology (RSM) were different for each type of material, so process optimization proved to be necessary. Considering that tobacco waste is mostly discarded or not effectively utilized, the results clearly show that tobacco waste could be used as a potential source of some bioactive compounds.

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

scholarly journals Ultrasound-assisted extraction of bioactive compounds from lemon balm and peppermint leaves

2016 ◽  
Vol 30 (1) ◽  
pp. 95-104 ◽  
Author(s):  
Jana Šic Žlabur ◽  
Sandra Voća ◽  
Nadica Dobričević ◽  
Stjepan Pliestić ◽  
Ante Galić ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Ultrasonic Extraction ◽  
Total Phenolic ◽  
Total Phenols ◽  
Ultrasound Assisted Extraction ◽  
Lemon Balm ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Abstract The aim of this study was to investigate the influence of conventional and ultrasound-assisted extraction (frequency, time, temperature) on the content of bioactive compounds as well as on the antioxidant activity of aqueous extracts from fresh lemon balm and peppermint leaves. Total phenols, flavonoids, non-flavonoids, total chlorophylls, total carotenoids, and radical scavenging capacity were determined. Moreover, the relationship between bioactive compounds and antioxidant capacity was studied by linear regression. A significant increase in all studied bioactive compounds during ultrasonic extraction for 5 to 20 min was found. With the classical extraction method, the highest amounts of total phenols, flavonoids, and antioxidant activity were determined, and the maximum amounts of total chlorophylls and carotenoids were determined during 20 min ultrasonic extraction. The correlation analysis revealed a strong, positive relationship between antioxidant activity and total phenolic compounds.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phenolic Compounds from Annona muricata By-Products and Pulp

Molecules ◽  
2019 ◽  
Vol 24 (5) ◽  
pp. 904 ◽  
Author(s):  
Gabriela Aguilar-Hernández ◽  
María García-Magaña ◽  
María Vivar-Vera ◽  
Sonia Sáyago-Ayerdi ◽  
Jorge Sánchez-Burgos ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Raw Materials ◽  
Raw Material ◽  
Ultrasound Assisted Extraction ◽  
Total Polyphenols ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Conventional Extraction ◽  
By Products

Ultrasound-assisted extraction (UAE) is widely used; however, the efficiency of extraction depends on the raw materials. Therefore, optimization of UAE must be investigated for each type of plant material. By-products from soursop fruit have not been studied as a source of bioactive compounds. In this work, the optimization of UAE conditions (extraction time (5, 10, and 15 min), pulse cycle (0.4, 0.7, and 1 s), and sonication amplitude (40%, 70%, and 100%)) for the extraction of phenolic compounds (soluble, hydrolyzable, condensed tannins, and total polyphenols) from soursop by-products (seed, peel, and columella) and pulp was evaluated using response surface methodology. The optimal conditions for UAE to obtain the highest total polyphenol content from by-products and pulp was dependent on the raw material. Peel resulted in the highest content of total polyphenols (187.32 mg/g dry matter [DM]) followed by columella (164.14 mg/g DM), seed (36.15 mg/g DM), and pulp (33.24 mg/g DM). The yield of polyphenolic content from peel and columella obtained with UAE was higher (32–37%) than conventional extraction for 2 h under stirring (14–16%). The contents of gallic acid (0.36–15.86 µg/g DM), coumaric acid (0.07–1.37 µg/g DM), and chlorogenic acid (9.18–32.67 µg/g DM) in the different parts of the fruit were higher in the extracts obtained by UAE compared with a conventional extraction method (0.08–0.61, 0.05–0.08, 3.15–13.08 µg/g DM, respectively), although it was dependent on the raw materials. Soursop by-products can be functionally important if they are used to extract bioactive compounds by UAE; a technology with high potential for commercial extraction on a large scale.

scholarly journals The Chemical and Biological Profiles of Leaves from Commercial Blueberry Varieties

Plants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1193
Author(s):  
Bianca-Eugenia Ștefănescu ◽  
Lavinia Florina Călinoiu ◽  
Floricuța Ranga ◽  
Florinela Fetea ◽  
Andrei Mocan ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Biological Activities ◽  
Vaccinium Corymbosum ◽  
World Health ◽  
Ultrasound Assisted Extraction ◽  
Leaf Extracts ◽  
Extraction Technology ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Health Organization

Blueberries have seen an ascending production line boosted by World Health Organization (WHO) approvals for their contributions to a healthy diet and the evidence that they act against different diseases. This increase resulted in significant amounts of discarded leaves, which could be a valuable source of bioactive compounds. In the present study, ultrasound-assisted extraction technology was used to determine and compare the chemical and biological profiles of leaves from six commercial blueberry (Vaccinium corymbosum L.) varieties. Feruloylquinic acid was the major compound identified, ranging from 19.23 ± 0.18 mg/g (at the lowest level, registered in the Spartan variety) to 49.62 ± 0.41 mg/g (at the highest level, registered in the Nelson variety). Rutin was the second major compound identified, for which Toro, Nelson, and Elliot leaves registered the highest values, with 35.77 ± 0.19 mg/g, 32.50 ± 0.20 mg/g, and 31.53 ± 0.1 mg/g, respectively. Even though analogous polyphenols were detected in the six cultivars, their concentrations and amounts were different. The leaf extracts of the cultivars Toro, Elliot, and Nelson appear to be good sources of antioxidants, registering high percentage inhibitions of DPPH radicals, of 70.41%, 68.42%, and 58.69%, respectively. The blueberry leaf extracts had a strong antibacterial activity and a low antifungal capacity, and a low-to-moderate antimutagenic capacity towards Salmonella typhimurium TA98 and TA100 strains, with Toro leaf being the best candidate. All of these biological activities indicate health-related benefits, recommending them as suitable candidates for medical and pharmaceutical applications. The present paper adds significant knowledge to the field of blueberry leaves via chemical and biological profiles, supporting the ultrasound-assisted extraction technique as a useful and green method to provide alternative sources of bioactive compounds.

scholarly journals Ultrasound-Assisted Extraction of Bioactive Compounds from Annatto Seeds, Evaluation of Their Antimicrobial and Antioxidant Activity, and Identification of Main Compounds by LC/ESI-MS Analysis

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Julian Quintero Quiroz ◽  
Ana Maria Naranjo Duran ◽  
Mariluz Silva Garcia ◽  
Gelmy Luz Ciro Gomez ◽  
John Jairo Rojas Camargo
Keyword(s):  
Staphylococcus Aureus ◽  
Antioxidant Activity ◽  
Bacillus Cereus ◽  
Bioactive Compounds ◽  
Treatment Time ◽  
Solvent Ratio ◽  
Ultrasound Assisted Extraction ◽  
Esi Ms ◽  
Assisted Extraction ◽  
Ultrasound Assisted

This study evaluated the antimicrobial activity (i.e., against Bacillus cereus and Staphylococcus aureus) and the antioxidant activity (i.e., ABTS, FRAP, and DPPH) of annatto seeds extract obtained by ultrasound-assisted extraction. A response surface design with three levels such as pH (2-11), solvent concentration (50-96 %), seed-to-solvent ratio (1:2–1:10), and treatment time (0-30 min) was employed to determine the optimal experimental conditions. Thus, a pH of 7.0, seed-to-solvent ratio of 1:7, and treatment time of 20 min were selected as optimal rendering an extract having a 0.62% of bixin, 3.81 mg gallic acid/mg equivalent of polyphenol compounds (ABTS 1035.7, FRAP 424.7, and DPPH 1161.5 μM trolox/L), and a minimal inhibitory concentration against Bacillus cereus and Staphylococcus aureus of 32 and 16 mg/L, respectively. Further, the main bioactive compounds identified by LC/ESI-MS were bixin and catechin, chlorogenic acid, chrysin, butein, hypolaetin, licochalcone A, and xanthohumol.

scholarly journals Antioxidant activity of St. John’s Wort extracts obtained with ultrasound-assisted extraction

2019 ◽  
Vol 65 (4) ◽  
Author(s):  
Anna Muzykiewicz ◽  
Katarzyna Florkowska ◽  
Anna Nowak ◽  
Joanna Zielonka-Brzezicka ◽  
Adam Klimowicz
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Properties ◽  
Raw Material ◽  
Aqueous Extracts ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
St John’S Wort ◽  
St John's Wort ◽  
Assisted Extraction ◽  
Ultrasound Assisted

Introduction: St. John’s Wort (Hypericum perforatum L.) is a plant from the Hypericaceae family commonly occurring in Europe, Asia, Africa and North America. It is used for medical purposes, for example in depression, viral or bacterial diseases, as well as diseases of the digestive system. St. John’s Wort is a source of hypericin as well as flavonoids useful to reduce the harmful effects of free radical action.The aim of the study was to evaluate the antioxidant activity of alcoholic and aqueous extracts of H. perforatum.Materials and methods: St. John’s Wort extracts were prepared in ethanol, methanol and isopropanol (each of 40%, 70% (v/v) and undiluted) and in water, with ultrasound-assisted extraction. Plant samples were extracted for 15, 30 and 60 min, and the antioxidant potentials of the extracts evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) aand ferric reducing antioxidant power (FRAP).Results: All the extracts showed antioxidant activity. In the case of the DPPH evaluation, the results ranged 2.42–4.37 mg trolox/g of raw material, which corresponds to 53.89–91.98% radical scavenging activity (RSA). The highest results were obtained for samples extracted for 60 min, with the antioxidant activity of most of them above 4.00 mg trolox/g of raw material. In the case of FRAP evaluation, the reducing power of the extracts ranged 1.40–37.82 mg FeSO4/g of raw material, with the highest results found for samples prepared in undiluted methanol. In contrast, the antioxidant capacity of the aqueous extracts was low.Conclusions: St. John’s Wort herb showed high antioxidant activity. In most cases, higher potentials were found for the extracts prepared in undiluted alcohols. The type of solvent used and the extraction time influenced the antioxidant properties of the extracts.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Bioactive Compounds from Pelvetia canaliculata to Sunflower Oil

Foods ◽  
2021 ◽  
Vol 10 (8) ◽  
pp. 1732
Author(s):  
Gabriela Sousa ◽  
Marija Trifunovska ◽  
Madalena Antunes ◽  
Isabel Miranda ◽  
Margarida Moldão ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oxidative Stability ◽  
Bioactive Compounds ◽  
Sunflower Oil ◽  
Oxidation Products ◽  
Total Phenolic ◽  
Ultrasound Assisted Extraction ◽  
Antioxidant Power ◽  
Assisted Extraction ◽  
Ultrasound Assisted

In this study, Pelvetia canaliculata L. macroalga, collected from the Atlantic Portuguese coast, was used as a source of bioactive compounds, mostly antioxidants, to incorporate them in sunflower oil with the aim of increasing its biological value and oxidative stability. The lyophilized alga was added to the oil, and ultrasound-assisted extraction (UAE) was performed. Algae concentration and UAE time varied following a central composite rotatable design (CCRD) to optimize extraction conditions. The following parameters were analyzed in the oils: oxidation products, acidity, color, chlorophyll pigments, carotenoids, flavonoids, total phenolic content, antioxidant activity by DPPH (2,2-diphenyl-1-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) assays, and sensory analysis. Extraction conditions did not affect the acidity and the amount of oxidation products in the oil. Chlorophylls and carotenoid contents increased with algae concentration, while flavonoid extraction did not depend on algae content or UAE time. Total phenolics in the oil were highly related only to FRAP antioxidant activity. Storage experiments of supplemented oil (12.5% algae; 20 min UAE) were carried out under accelerated oxidation conditions at 60 °C/12 days. Antioxidant activity (FRAP) of supplemented oil was 6-fold higher than the value of non-supplemented oil. Final samples retained 40% of their initial antioxidant activity. The presence of algae extracts contributed to the increased oxidative stability of sunflower oil.

scholarly journals Optimization of Ultrasound-Assisted Extraction of Phloretin and Other Phenolic Compounds from Apple Tree Leaves (Malus domestica Borkh.) and Comparison of Different Cultivars from Estonia

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 189
Author(s):  
Sana Ben-Othman ◽  
Hedi Kaldmäe ◽  
Reelika Rätsep ◽  
Uko Bleive ◽  
Alar Aluvee ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Apple Tree ◽  
Maximum Yield ◽  
Plant Secondary Metabolites ◽  
Polyphenolic Compounds ◽  
Tree Leaves ◽  
Ultrasound Assisted Extraction ◽  
Assisted Extraction ◽  
Ultrasound Assisted ◽  
Promising Source

Polyphenolic compounds, plant secondary metabolites essential for plant survival, are known for their high antioxidant and anti-inflammatory activity. In addition, several polyphenols, such as phloretin, also have potential antiviral effects, making these compounds potential ingredients of biofunctional foods. A promising source for the extraction of phloretin is a by-product of apple production—apple tree leaves. Focusing on green technologies, the first aim of the present study was to optimize the direct ultrasound-assisted extraction conditions to gain the maximum yield of phloretin from air-dried apple leaves. For the optimization of process parameters, we applied the response surface method with Box–Behnken design. The optimal extraction conditions were extraction time 14.4 min, sonication amplitude 10% and 10 g of sample per 100 mL solvent (70% ethanol, w/w). Using these conditions, we assessed the content of individual and total polyphenolic compounds along with antioxidant activity in the leaves of different autumn and winter apple cultivars grown in Estonia. The analyses were carried out with chromatographic (HPLC-DAD-MS/MS) and spectrophotometric methods. The phloretin concentration ranged from 292 to 726 µg/g and antioxidant activity from 6.06 to 11.42 mg GA eq./g, these being the highest in the local winter cultivars ‘Paide taliõun’ and ‘Tellissaare’, respectively.

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