scholarly journals Changes in Beneficial C-glycosylflavones and Policosanol Content in Wheat and Barley Sprouts Subjected to Differential LED Light Conditions

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1502
Author(s):  
Muthusamy Muthusamy ◽  
Jong Hee Kim ◽  
Suk Hee Kim ◽  
Joo Yeol Kim ◽  
Jeong Wook Heo ◽  
...  

The spectral quality and intensity of light, photoperiodism, and other environmental factors have profound impacts on the metabolic composition of light-dependent higher plants. Hence, we investigate the effects of fluorescent light (96 μmol m−2s−1) and white (100 μmol m−2s−1), blue (100 μmol m−2s−1), and red (93 μmol m−2s−1) light-emitting diode (LED) light irradiation on the C-glycosylflavone and policosanol contents in young seedlings of wheat and barley. Ultra-high-performance liquid chromatography (UHPLC) analyses of C-glycosylflavone contents in barley reveal that the saponarin content is significantly enhanced under blue LED light irradiation. Under similar conditions, isoorientin and isoschaftoside contents are improved in wheat seedlings. The contents of these C-glycosylflavones differed along with the light quality and growth period. The highest accumulation was observed in sprouts after three days under blue LED light irradiation. GC/MS analyses of policosanol contents showed that 1-hexacosanol (C26:o–OH) in barley and 1-octacosanol (C28:o–OH) in wheat seedlings were reduced under LED light irradiation, compared to seedlings under fluorescent light conditions. Nonetheless, the policosanol contents gradually improved with the extension of growth times and treatments, irrespective of the light quality. Additionally, a positive correlation was observed between the expression pattern of biosynthesis-related genes and the respective metabolite content in barley. This study demonstrates that blue LED light irradiation is useful in maximizing the C-glycosylflavone content in barley and wheat sprouts.

2012 ◽  
Vol 40 (2) ◽  
pp. 31 ◽  
Author(s):  
Stefania ASTOLFI ◽  
Chiara MARIANELLO ◽  
Stefano GREGO ◽  
Rosanna BELLAROSA

The influence of light quality on growth and metabolic activity during pre-cultivation (in miniplug containers) of beech (Fagus sylvatica L.), holm oak (Quercus ilex L.) and wild cherry (Prunus avium) plants was investigated. Seedlings were grown in a growth chamber for a month under light-emitting diode (LED) light or fluorescent light. The LED lamps (Valoya) used in this study emitted a continuous spectrum thanks to a mixture of blue, green, red and far-red LEDs. Our results showed that plant response to light quality seems to be related to the plant species. In particular, in beech seedlings fresh and dry weight, shoot height and leaf area were greatest when plants were cultured under LED light, and lowest under fluorescent lamps. Furthermore, we found that LED-induced reduction of chlorophyll contents in beech and holm oak leaves resulted in an increase of the carboxylase capacity of Rubisco in the same plant species suggesting an improvement of light-use-efficiency in these plants. These results indicate that LED light may be suitable for the culture of plants in tightly controlled environments. The comparison of malondialdehyde levels between LED and fluorescent grown plants strongly supports this idea.


2021 ◽  
pp. 9-14
Author(s):  
B. Leoni ◽  
O.D. Palmitessa ◽  
F. Serio ◽  
A. Signore ◽  
P. Santamaria

2014 ◽  
Vol 94 ◽  
pp. 97-103 ◽  
Author(s):  
Gang Ma ◽  
Lancui Zhang ◽  
Chandra Kurnia Setiawan ◽  
Kazuki Yamawaki ◽  
Tatsuo Asai ◽  
...  

Plant Science ◽  
2015 ◽  
Vol 233 ◽  
pp. 134-142 ◽  
Author(s):  
Lancui Zhang ◽  
Gang Ma ◽  
Kazuki Yamawaki ◽  
Yoshinori Ikoma ◽  
Hikaru Matsumoto ◽  
...  

Biomolecules ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1423
Author(s):  
Yuan Li ◽  
Zong Jie Cui

Cholecystokinin 1 receptor (CCK1R) is activated by singlet oxygen (1O2) generated in photodynamic action with sulphonated aluminum phthalocyanine (SALPC) or genetically encoded protein photosensitizer (GEPP) KillerRed or mini singlet oxygen generator (miniSOG). A large number of GEPP with varied 1O2 quantum yields have appeared recently; therefore, in the present work, the efficacy of different GEPP to photodynamically activate CCK1R was examined, as monitored by Fura-2 calcium imaging. KillerRed, miniSOG, miniSOG2, singlet oxygen protein photosensitizer (SOPP), flavin-binding fluorescent protein from Methylobacterium radiotolerans with point mutation C71G (Mr4511C71G), and flavin-binding fluorescent protein from Dinoroseobacter shibae (DsFbFP) were expressed at the plasma membrane (PM) in AR4-2J cells, which express endogenous CCK1R. Light irradiation (KillerRed: white light 85.3 mW‧cm−2, 4’ and all others: LED 450 nm, 85 mW·cm−2, 1.5′) of GEPPPM-expressing AR4-2J was found to all trigger persistent calcium oscillations, a hallmark of permanent photodynamic CCK1R activation; DsFbFP was the least effective, due to poor expression. miniSOG was targeted to PM, mitochondria (MT) or lysosomes (LS) in AR4-2J in parallel experiments; LED light irradiation was found to all induce persistent calcium oscillations. In miniSOGPM-AR4-2J cells, light emitting diode (LED) light irradiation-induced calcium oscillations were readily inhibited by CCK1R antagonist devazepide 2 nM; miniSOGMT-AR4-2J cells were less susceptible, but miniSOGLS-AR4-2J cells were not inhibited. In conclusion, different GEPPPM could all photodynamically activate CCK1R. Intracellular GEPP photodynamic action may prove particularly suited to study intracellular GPCR.


2018 ◽  
Vol 14 ◽  
pp. 3047-3058 ◽  
Author(s):  
Atsushi Kaga ◽  
Xiangyang Wu ◽  
Joel Yi Jie Lim ◽  
Hirohito Hayashi ◽  
Yunpeng Lu ◽  
...  

The degenerative transfer of xanthates to olefins is enabled by the iridium-based photocatalyst [Ir{dF(CF3)ppy}2(dtbbpy)](PF6) under blue LED light irradiation. Detailed mechanistic investigations through kinetics and photophysical studies revealed that the process operates under a radical chain mechanism, which is initiated through triplet-sensitization of xanthates by the long-lived triplet state of the iridium-based photocatalyst.


Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 359 ◽  
Author(s):  
Gludia M. Maroga ◽  
Puffy Soundy ◽  
Dharini Sivakumar

The influence of emitting diode (LED) treatments for 8 h per day on functional quality of three types of fresh-cut sweet peppers (yellow, red, and green) were investigated after 3, 7, 11, and 14 days postharvest storage on the market shelf at 7 °C. Red LED light (660 nm, 150 μmol m−2 s−1) reduced weight loss to commercially acceptable level levels (≤2.0%) in fresh-cuts of yellow and green sweet peppers at 7 and 11 d, respectively. Blue LED light (450 nm, 100 μmol m−2 s−1) maintained weight loss acceptable for marketing in red fresh-cut sweet peppers up to 11 d. Highest marketability with minimum changes in color difference (∆E) and functional compounds (total phenols, ascorbic acid content, and antioxidant activity) were obtained in yellow and green sweet pepper fresh-cuts exposed to red LED light up to 7 and 11 d, respectively, and for red sweet pepper fresh-cuts exposed to blue LED light for 11 d. Red LED light maintained the highest concentrations of β carotene, chlorophyll, and lycopene in yellow, green, and red sweet pepper fresh-cuts up to 7 d. Similarly, blue LED light showed the highest increase in lycopene concentrations for red sweet pepper fresh-cuts up to 7 d. Red LED (yellow and green sweet peppers) and blue LED (red sweet pepper) lights maintained phenolic compounds by increasing phenylalanine ammonia lyase activity. Thus, the results indicate a new approach to improve functional compounds of different types of fresh-cut sweet pepper.


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