scholarly journals Advancements in Low-Chill Blueberry Vaccinium corymbosum L. Tissue Culture Practices

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1624
Author(s):  
Francesco Cappai ◽  
Alexandria Garcia ◽  
Ryan Cullen ◽  
Matthew Davis ◽  
Patricio R. Munoz
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Transformation ◽  
Vaccinium Corymbosum ◽  
White Led ◽  
Market Expansion ◽  
Culture Characteristics ◽  
Promote Plant Growth ◽  
Led Lights

The demand for blueberry Vaccinium corymbosum L. (and hybrids) plants has significantly increased in the last 30 years due to its market expansion. In vitro propagation of sterile plants are required for commercial purposes but also for research applications such as plant transformation. Thus far, tissue culture characteristics of the tropical-adapted blueberry have been scarcely studied. In this study we present the following findings: (i) zeatin, a hormone used to promote plant growth, should be used in the 1–2 mg/L range to promote plant architecture optimal for transformation experiments; (ii) red-blue LED lights induce more production of meristems and biomass than white LED or fluorescent lights; (iii) levels as high as 1000 mg/L of decontamination agents (the antibiotics timentin and cefotaxime) can be used to eliminate Agrobacterium overgrowth without inhibiting plant growth during plant transformation experiments; (iv) kanamycin, paromomycin, and geneticin, which are widely used antibiotics to select transgene-carrying transformants, cannot be efficiently used in this system; (v) glufosinate, a widely used herbicide, shows potential to be used as an effective selectable marker for transformed plants.

scholarly journals The Effect of Plant Growth Regulators on Callus Induction and Regeneration of Amygdalus communis

2011 ◽  
Vol 3 (3) ◽  
pp. 97-100
Author(s):  
Naimeh SHARIFMOGHADAM ◽  
Abbas SAFARNEJAD ◽  
Sayed Mohammad TABATABAEI
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Base Material ◽  
Culture Technique ◽  
Induction Medium ◽  
Root Induction ◽  
Amygdalus Communis

The Almond (Amygdalus communis) is one of the most important and oldest commercial nut crops, belonging to the Rosaceae family. Almond has been used as base material in pharmaceutical, cosmetic, hygienically and food industry. Propagation by tissue culture technique is the most important one in woody plants. In the current research, in vitro optimization of tissue culture and mass production of almond was investigated. In this idea, explants of actively growing shoots were collected and sterilized, then transferred to MS medium with different concentrations and combinations of plant growth regulators. The experiment was done in completely randomized blocks design, with 7 treatment and 30 replications. After 4 weeks, calli induction, proliferation, shoot length and number of shoot per explants were measured. Results showed that the best medium for shoot initiation and proliferation was MS + 0.5 mg/l IAA (Indol-3-Acetic Acid) + 1 mg/l BA (Benzyl Adenine). Autumn was the best season for collecting explants. The shoots were transferred to root induction medium with different concentrations of plant growth regulators. The best root induction medium was MS + 0.5 mg/l IBA (Indol Butyric Acid).

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals Organogenesis in okra (Abelmoschus esculentus L. Moench.): A plant recalcitrant to tissue culture

2016 ◽  
Vol 41 (3) ◽  
pp. 521-528
Author(s):  
MR Kabir ◽  
S Ahmed ◽  
MAY Akhond
Keyword(s):  
Tissue Culture ◽  
Acetic Acid ◽  
Plant Growth ◽  
Root Induction ◽  
Ms Medium ◽  
Natural Conditions ◽  
Hypocotyl Explants ◽  
Cotyledonary Nodes ◽  
Dichlorophenoxy Acetic Acid

Seedling-derived cotyledonary nodes and hypocotyl explants of BARI Dherosh- 1 were cultured in vitro on MS medium supplemented with varying concentrations of 2, 4-Dichlorophenoxy acetic acid (2, 4-D), 6- Benzylaminopurine (BAP), Thidiazuron (TDZ), BAP with 1-Nepthaleneacetic acid (NAA), BAP with Indole 3-butyric acid (IAA) and Zeatin with IAA along with a control. Shooting response (100%) with callus was only observed from cotyledonary nodes on thidiazuron (TDZ) where hypocotyls produced only callus or callus with roots on different concentrations of plant growth regulators. Considering the shooting response, the cotyledonary nodes of BARI Dherosh-1 were cultured on various concentrations of TDZ for regeneration. The highest percentage (64.0) with maximum number (6.8) of shoots per explant were observed in 0.044 ?M TDZ in 8.4 days. The regenerated shoots were rooted on ½ strength MS, MS supplemented with 2.46 ?M IBA and 0.53 ?M NAA. The highest percentage (83.3) and minimum days (9.7) required for root induction were recorded in 2.46 ?M IBA. The rooted plantlets were transferred to soil and hardened in the plastic pots under green house conditions. The rooted shoots grew normally under natural conditions following acclimatization.Bangladesh J. Agril. Res. 41(3): 521-528, September 2016

Effect of two bio-polysaccharides on organogenesis of protocorm-like bodies in Phalaenopsis cultured in vitro

2020 ◽  
Vol 26 (1) ◽  
pp. 2137-2142
Author(s):  
A. M. Meskatul ◽  
K. Shimasaki ◽  
S. U. Habiba
Keyword(s):  
Shoot Formation ◽  
Vital Role ◽  
White Led ◽  
Fresh Weight ◽  
High Concentration ◽  
Low Concentrations ◽  
Different Types ◽  
Modified Ms Medium ◽  
Led Lights

Different types of bio-polysaccharide play a vital role in the growth of PLBs cultured in vitro. In this study, to we investigated the potential impacts of two bio-polymers,: hyaluronic acid (HA9) and sodium alginate (ALG) on the organogenesis of protocorm-like bodies (PLBs) in Phalaenopsis under white LED lights. PLBs of Phalaenopsis ‘Fmk02010’ were explanted on modified MS medium with different concentrations of HA and (ALG). The highest average number of PLBs per explant (24.6) was recorded for ALG alone at a concentration of 0.01mg/L, and the fresh weight was also highest at the same concentration. The combination of 0.01mg/L ALG and 0.01mg/L HA also resulted in a large number of PLBs (23.8) and high fresh weight. As opposed to, the highest number of shoots /explant (3.6) was observed at the treatment of the combination of 1mg/L ALG and 10mg/L HA. This study shows that the application of ALG and HA alone, and in combination, at low concentrations, increased the average number of PLBs and the amount of fresh weight, but shoot formation was higher at a high concentration compared with control.

scholarly journals Transplanting Space Effect on In-vitro Raised Sugarcane

2018 ◽  
Vol 10 (01) ◽  
pp. 12-16
Author(s):  
Sonali Gangwar ◽  
Saurabh Pathak ◽  
Maya Datt Joshi
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth And Yield ◽  
Cane Yield ◽  
Sugarcane Variety ◽  
Rapid Multiplication ◽  
Space Effect ◽  
Healthy Seed ◽  
Disease Free

Tissue cuture is considered to be a best technique for rapid multiplication and production of disease free, healthy seed cane. With a view to studying the effect of transplanting spacing on growth and yield of micropropagated crop of sugarcane, an experiment was carried out at Tissue Culture Laboratory, Shobhit University, Meerut. Tissue culture raised plantlets of sugarcane variety. In vitro cultured sugarcane were transplanted at various spacing of 90 x 45, 90 x 60, 90 x 90 and 120 x 60 cm. Among the four spacings, the highest plant growth, number of tillers, number of malleable canes, cane height and cane yield were recorded at 90 x 60 cm. Thus, a spacing of 90 x 60 cm was found most suitable for transplantation of tissue culture raised plantlets of sugarcane.

scholarly journals 664 PB 104 AGROBACTERIUM INFECTION OF WHITE ASH (FRAXINUS AMERICANA L.)

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 528a-528
Author(s):  
Sharon A. Bates ◽  
John E. Preece ◽  
John H. Yopp
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulators ◽  
Callus Formation ◽  
High Humidity ◽  
Ms Medium ◽  
Gall Formation ◽  
White Ash ◽  
Seedling Explants

Both greenhouse-grown white ash plants derived from tissue culture and rooted microshoots in high humidity trays were inoculated with 11 tumor-inducing Agrobacterium strains. Eight strains stimulated mutative gall formation. Plants inoculated with strain A281 exhibited a higher frequency of callus formation (greenhouse-22.2%; microshoots-18.8%) than other strains at the site of the wound. Therefore, strain A281 was used to inoculate seed and seedling explants in vitro. Explants were placed on MS medium containiner no plant growth regulators and inoculated at 0, 3, 5, 7, or 10 days after initiation. Plants inoculated at 10 days showed a higher frequency of callus formation (16.4%) than with earlier inoculations. Also, rewounding of the explant at inoculation resulted in a higher frequency of callus formation (11.3%) compared to not rewounding the explant (3.9%).

Isolation and characterization of cassava root endophytic bacteria with the ability to promote plant growth and control the in vitro and in vivo growth of Phytopythium sp.

2021 ◽  
pp. 101709
Author(s):  
Solange da Cunha Ferreira ◽  
Alessandra Keiko Nakasone ◽  
Silvia Mara Coelho do Nascimento ◽  
Danyllo Amaral de Oliveira ◽  
Andrei Santos Siqueira ◽  
...  
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Isolation And Characterization ◽  
Cassava Root ◽  
Promote Plant Growth ◽  
In Vivo Growth ◽  
And Control

Strategies to propagate Vaccinium nuclear stocks for the Canadian berry industry

2007 ◽  
Vol 87 (4) ◽  
pp. 911-922 ◽  
Author(s):  
Samir C Debnath
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
In Vitro Propagation ◽  
Adventitious Shoot ◽  
Commercial Production ◽  
Gene Conservation ◽  
Mass Propagation ◽  
Adventitious Shoot Regeneration ◽  
Clonal Multiplication

Vacinium fruits are genetically heterozygous species characterized as “not coming true from seed”. Conventional methods for vegetative propagation of these species, although successful, are slow and labour-intensive, and few propagules can be produced from one plant of a selected clone or hybrid. Micropropagation techniques are important for clonal multiplication, germplasm im provement and gene conservation of Vaccinium fruits cultivated in Canada including blueberries, cranberries and lingonberries. In vitro propagation of these species using axillary bud proliferation and adventitious shoot regeneration has been investigated in a number of studies. Morphogenesis seems to be highly dependent on plant growth regulators and media used for culture, and this dependence is genotype specific. The paper presents the progress in-depth of various aspects of the in vitro culture of Canadian Vaccinium species for their commercial production. Also discussed are techniques for clone rejuvenation and plant tissue culture for mass propagation of Canadian Vaccinium nuclear stocks. Key words: Blueberry, cranberry, lingonberry, micropropagation, regeneration, morphology

scholarly journals In vitro shoot multiplication of Morus nigra by combinations of plant growth regulators

2021 ◽  
Vol 921 (1) ◽  
pp. 012036
Author(s):  
S H Larekeng ◽  
M A Arsyad ◽  
A M Annisa ◽  
M Restu
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Shoot Multiplication ◽  
Callus Cultures ◽  
Leaf Length ◽  
Plant Parts ◽  
Morus Nigra ◽  
Conventional Technology ◽  
The Right

Abstract Mulberry (Morus nigra L) is a dicotyledonous plant in the family Moraceae which often used for silkworms’ breeding. Mulberry propagation still uses conventional technology, such as cuttings and graftings. The problem of mulberry propagation is the low productivity of mulberry gardens. However, tissue culture is a technique of isolating plant parts in the form of organs, cell tissues, and protoplasms that is effective and efficient in order to get uniform and superior plants in a short amount of time. For mulberry growth, the right combination of the Plant Growth Regulator (PGR) in the form of Kinetin, IAA, IBA is needed. Data were analyzed using R-statistic software. The results showed that M6 media (MS + Kinetin 1.5 + 1 IAA) was the best combination of media for the number of shoots, leaf length, and number of roots, with 80% of live explant percentage. Propagation via tissue culture such as clonal propagation, organogenesis, and somatic embryogenesis in callus cultures followed by generation of shoots/plantlets represents a potential effective propagation method in the future.

scholarly journals Evaluation potential of PGPR to protect tomato against Fusarium wilt and promote plant growth

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11194
Author(s):  
Rizwana begum Syed Nabi ◽  
Raheem Shahzad ◽  
Rupesh Tayade ◽  
Muhammad Shahid ◽  
Adil Hussain ◽  
...  
Keyword(s):  
Plant Growth ◽  
Fungal Growth ◽  
Significant Inhibition ◽  
Wilt Disease ◽  
Vegetable Crops ◽  
Concomitant Reduction ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Endogenous Phytohormones

Soilborne fungal diseases are most common among vegetable crops and have major implications for crop yield and productivity. Eco-friendly sustainable agriculture practices that can overcome biotic and abiotic stresses are of prime importance. In this study, we evaluated the ability of plant growth-promoting rhizobacterium (PGPR) Bacillus aryabhattai strain SRB02 to control the effects of tomato wilt disease caused by Fusarium oxysporum f. sp. lycopersici (strain KACC40032) and promote plant growth. In vitro bioassays showed significant inhibition of fungal growth by SRB02. Inoculation of susceptible and tolerant tomato cultivars in the presence of SRB02 showed significant protection of the cultivar that was susceptible to infection and promotion of plant growth and biomass production in both of the cultivars. Further analysis of SRB02-treated plants revealed a significantly higher production of amino acids following infection by F. oxysporum. Analysis of plant defense hormones after inoculation by the pathogen revealed a significantly higher accumulation of salicylic acid (SA), with a concomitant reduction in jasmonic acid (JA). These results indicate that B. aryabhattai strain SRB02 reduces the effects of Fusarium wilt disease in tomato by modulating endogenous phytohormones and amino acid levels.

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