scholarly journals Evaluation potential of PGPR to protect tomato against Fusarium wilt and promote plant growth

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11194
Author(s):  
Rizwana begum Syed Nabi ◽  
Raheem Shahzad ◽  
Rupesh Tayade ◽  
Muhammad Shahid ◽  
Adil Hussain ◽  
...  
Keyword(s):  
Plant Growth ◽  
Fungal Growth ◽  
Significant Inhibition ◽  
Wilt Disease ◽  
Vegetable Crops ◽  
Concomitant Reduction ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Endogenous Phytohormones

Soilborne fungal diseases are most common among vegetable crops and have major implications for crop yield and productivity. Eco-friendly sustainable agriculture practices that can overcome biotic and abiotic stresses are of prime importance. In this study, we evaluated the ability of plant growth-promoting rhizobacterium (PGPR) Bacillus aryabhattai strain SRB02 to control the effects of tomato wilt disease caused by Fusarium oxysporum f. sp. lycopersici (strain KACC40032) and promote plant growth. In vitro bioassays showed significant inhibition of fungal growth by SRB02. Inoculation of susceptible and tolerant tomato cultivars in the presence of SRB02 showed significant protection of the cultivar that was susceptible to infection and promotion of plant growth and biomass production in both of the cultivars. Further analysis of SRB02-treated plants revealed a significantly higher production of amino acids following infection by F. oxysporum. Analysis of plant defense hormones after inoculation by the pathogen revealed a significantly higher accumulation of salicylic acid (SA), with a concomitant reduction in jasmonic acid (JA). These results indicate that B. aryabhattai strain SRB02 reduces the effects of Fusarium wilt disease in tomato by modulating endogenous phytohormones and amino acid levels.

scholarly journals In vitro Screening and Optimization of IAA Production from Plant Growth Promoting Rhizobacteria Burkholderia cepacia UPMB3

2018 ◽  
Vol 28 (1) ◽  
pp. 25-34
Author(s):  
Waheeda Parvin ◽  
Quazi Shirin Akhter Jahan ◽  
Md Mahbubur Rahman ◽  
Mui Yun Wong
Keyword(s):  
Plant Growth ◽  
Oil Palm ◽  
Burkholderia Cepacia ◽  
Plant Growth Promoting Rhizobacteria ◽  
Leaf Length ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Layer Chromatography

Burkholderia cepacia UPMB3 is an important plant growth promoting rhizobacteria isolated from oil palm rhizosphere which is considered to promote plant growth directly or indirectly. The IAA was extracted, purified, detected and confirmed by thin layer chromatography analyses f r om t h e strain UPMB3 of B. cepacia. Rf value was compared with the authentic IAA. Maximum 50 μg/ml IAA was produced in the medium supplemented with 4 mg/ml L-tryptophan, under shaken conditions at 150 rpm in seven days incubation at pH 7. The bacterial extract significantly influenced the growth of oil palm seedlings producing shoot, root, leaf and leaf length compared to control.Plant Tissue Cult. & Biotech. 28(1): 25-34, 2018 (June)

scholarly journals Rhizobacteria Associated with a Native Solanaceae Promote Plant Growth and Decrease the Effects of Fusariumoxysporum in Tomato

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 579
Author(s):  
Carmen Sanjuana Delgado-Ramírez ◽  
Rufina Hernández-Martínez ◽  
Edgardo Sepúlveda
Keyword(s):  
Plant Growth ◽  
Fusarium Wilt ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Positive Effects ◽  
Alternative Approach ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Plant growth-promoting rhizobacteria are often utilized to improve crop health and productivity. Nevertheless, their positive effects can be hindered if they fail to withstand the environmental and ecological conditions of the regions where they are applied. An alternative approach to circumvent this problem is a tailored selection of bacteria for specific agricultural systems. In this work, we evaluated the plant growth promoting and pathogen inhibition activity of rhizobacteria obtained from the rhizosphere of Mariola (Solanum hindsianum), an endemic shrub from Baja California. Eight strains were capable of inhibiting Fusarium oxysporum in vitro, and thirteen strains were found to possess three or more plant-growth-promotion traits. Molecular identification of these strains, using 16 s rRNA partial sequences, identified them as belonging to the genera Arthrobacter, Bacillus, Paenibacillus, Pseudomonas, and Streptomyces. Finally, the effect of selected plant growth-promoting rhizobacteria (PGPR) strains on the growth and suppression of Fusarium wilt in tomato was evaluated. Results showed that these strains improved tomato plants growth under greenhouse conditions and reduced Fusarium wilt effects, as reflected in several variables such as length and weight of roots and stem. This work highlights the potential of native plants related to regionally important crops as a valuable source of beneficial bacteria.

scholarly journals Bioprospecting of elite plant growth-promoting bacteria for the maize crop

2020 ◽  
Vol 42 ◽  
pp. e44364 ◽  
Author(s):  
Angela Cristina Ikeda ◽  
Daiani Cristina Savi ◽  
Mariangela Hungria ◽  
Vanessa Kava ◽  
Chirlei Glienke ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Maize Crop ◽  
Agriculture Sustainability ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Biological Nitrogen

The use of plant growth-promoting bacteria (PGPB), which aims to replace chemical fertilizers and biological control, is a goal for achieving agriculture sustainability. In this scenario, our goal was to identify and evaluate the potential of bacteria isolated from maize roots to promote plant growth and be used as inoculants. We evaluated 173 bacterial strains isolated from the maize (Zea mays L.) rhizosphere for the properties of their PGPB in vitro. Twelve strains were positive for siderophores, indole acetic acid (IAA) production, biological nitrogen fixation (BNF), and phosphate solubilization. Sequence analysis of 16S rRNA identified these strains as belonging to the genera Cellulosimicrobium, Stenotrophomonas, Enterobacter, and Bacillus. The elite strains were evaluated under greenhouse conditions upon the inoculation of two maize hybrids, ATL100 and KWX628. The ability of the isolates to promote plant growth was dependent on the maize genotype; Enterobacter sp. LGMB208 showed the best ability to promote growth of hybrid ATL100, while Enterobacter sp. strains LGMB125, LGMB225, and LGMB274 and Cellulosimicrobium sp. strain LGMB239 showed the best ability to promote growth of hybrid KWX628. The results highlight the potential of bacterial genera little explored as maize PGPB but indicate the need to investigate their interactions with different plant genotypes.

scholarly journals Isolation and identification of chromium-tolerant bacterial strains and their potential to promote plant growth

2019 ◽  
Vol 96 ◽  
pp. 01005
Author(s):  
Muhammad Arshad ◽  
Anza Javaid ◽  
Maria Manzoor ◽  
Kiran Hina ◽  
Muhammad Arif Ali ◽  
...  
Keyword(s):  
Plant Growth ◽  
Contaminated Soils ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Pseudomonas Plecoglossicida ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting

Remediation and management of Chromium (Cr) contaminated soils for safe usage has been a serious challenge. The aim of present work was to isolate and identify Cr tolerant bacterial strains and assessing their plant growth promoting potential under controlled conditions. Soil samples were collected from Gujrat District, Pakistan, and analyzed for Cr content and bacteria isolation. In-vitro screening was done for chromium tolerance and plant growth-promoting (PGP) abilities. The tolerant isolates which also exhibited PGP abilities were used as inoculants in germination and pot trial experiment. The isolates A5 and A6, identified as Pseudomonas plecoglossicida and Staphylococci saprophyticus through 16S rRNA gene sequencing, were found to be tolerant up to 700 mg L-1 of Cr (VI). Both were effective in solubilizing phosphate but only A5 (Pseudomonas plecoglossicida) was able to produce indole acetic acid. A5 also increased the percentage of seed germination from 17 to 46% and spinach plant’s biomass by 44% with respect to the control. Keeping in view the results obtained, A5 (Pseudomonas plecoglossicida) appeared as the best species that was able to tolerate Cr stress and promote plant growth. Further, it can be developed as a bio-inoculant for non-food agricultural applications for remediation of Cr contaminated soils.

A Study on the Farmers’ Awareness and Acceptance of Biofertilizers in Kottayam District

GIS Business ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. 425-431
Author(s):  
Subin Thomas ◽  
Dr. M. Nandhini
Keyword(s):  
Organic Matter ◽  
Nitrogen Fixation ◽  
Plant Growth ◽  
Plant Growth Promoting Rhizobacteria ◽  
Nutrient Cycle ◽  
Plant Growth Promoting ◽  
Promote Plant Growth ◽  
Growth Promoting ◽  
Structured Questionnaire ◽  
Area Data

Biofertilizers are fertilizers containing microorganisms that promote plant growth by improving the supply of nutrients to the host plant. The supply of nutrients is improved naturally by nitrogen fixation and solubilizing phosphorus. The living microorganisms in biofertilizers help in building organic matter in the soil and restoring the natural nutrient cycle. Biofertilizers can be grouped into Nitrogen-fixing biofertilizers, Phosphorous-solubilizing biofertilizers, Phosphorous-mobilizing biofertilizers, Biofertilizers for micro nutrients and Plant growth promoting rhizobacteria. This study conducted in Kottayam district was intended to identify the awareness and acceptance of biofertilizers among the farmers of the area. Data have been collected from 120 farmers by direct interviews with structured questionnaire.

scholarly journals Crude Citric Acid of Trichoderma asperellum: Tomato Growth Promotor and Suppressor of Fusarium oxysporum f. sp. lycopersici

Plants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 222
Author(s):  
Abdulaziz A. Al-Askar ◽  
WesamEldin I. A. Saber ◽  
Khalid M. Ghoneem ◽  
Elsayed E. Hafez ◽  
Amira A. Ibrahim
Keyword(s):  
Citric Acid ◽  
Plant Growth ◽  
Organic Acid ◽  
Wilt Disease ◽  
Growth And Yield ◽  
Total Phenolic ◽  
Growth Promoters ◽  
Chlorophyll Pigments

Presently, the bioprocessing of agricultural residues to various bioactive compounds is of great concern, with the potential to be used as plant growth promoters and as a reductive of various diseases. Lycopersiconesculentum, one of the most consumed crops in the human diet, is attacked by Fusarium wilt disease, so the main aim is to biocontrol the pathogen. Several fungal species were isolated from decayed maize stover (MS). Trichodermaasperellum was chosen based on its organic acid productivity and was molecularly identified (GenBank accession number is MW195019). Citric acid (CA) was the major detected organic acid by HPLC. In vitro, CA of T.asperellum at 75% completely repressed the growth of Fusariumoxysporum f. sp. lycopersici (FOL). In vivo, soaking tomato seeds in CA enhanced the seed germination and vigor index. T. asperellum and/or its CA suppressed the wilt disease caused by FOL compared to control. There was a proportional increment of plant growth and yield, as well as improvements in the biochemical parameters (chlorophyll pigments, total phenolic contents and peroxidase, and polyphenol oxidase activities), suggesting targeting both the bioconversion of MS into CA and biological control of FOL.

scholarly journals Activity of plant growth promoting rhizobacteria (PGPRs) in the biocontrol of tomato Fusarium wilt

2017 ◽  
Vol 53 (No. 2) ◽  
pp. 78-84 ◽  
Author(s):  
Boukerma Lamia ◽  
Benchabane Messaoud ◽  
Charif Ahmed ◽  
Khélif Lakhdar
Keyword(s):  
Fusarium Wilt ◽  
Plant Growth Promoting Rhizobacteria ◽  
Significant Inhibition ◽  
Systemic Resistance ◽  
In Situ Experiment ◽  
Fluorescent Pseudomonas ◽  
Split Root ◽  
Plant Growth Promoting ◽  
Severity Of The Disease

The potential of Pseudomonas fluorescens PF15 and Pseudomonas putida PP27 to protect tomato plants against Fusarium wilt under greenhouse conditions was evaluated. In vitro antagonism showed a significant inhibition of the pathogen growth (47%) revealed by PF15. However, PP27 presented a 10% rate of the mycelium inhibition. An in situ experiment was conducted with split-root design for induced systemic resistance (ISR) and without split-root design to measure both ISR and antagonistic activities. Fluorescent Pseudomonas revealed a delay in the onset of symptoms and slower kinetics of disease progression compared to the pathogen control. McKinney’s index, which measures the severity of the disease, was reduced by 37–72%, and the levels of infection (incidence) by 7–36%.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals Multi-Trait Wheat Rhizobacteria from Calcareous Soil with Biocontrol Activity Promote Plant Growth and Mitigate Salinity Stress

2021 ◽  
Vol 9 (8) ◽  
pp. 1588
Author(s):  
Anastasia Venieraki ◽  
Styliani N. Chorianopoulou ◽  
Panagiotis Katinakis ◽  
Dimitris L. Bouranis
Keyword(s):  
Plant Growth ◽  
Salinity Stress ◽  
Calcareous Soil ◽  
In Vitro Studies ◽  
Pgp Traits ◽  
Biocontrol Activity ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Microbial Fertilizers

Plant growth promoting rhizobacteria (PGPR) can be functional microbial fertilizers and/or biological control agents, contributing to an eco-spirit and safe solution for chemical replacement. Therefore, we have isolated rhizospheric arylsulfatase (ARS)-producing bacteria, belonging to Pseudomonas and Bacillus genus, from durum wheat crop grown on calcareous soil. These isolates harbouring plant growth promoting (PGP) traits were further evaluated in vitro for additional PGP traits, including indole compounds production and biocontrol activity against phytopathogens, limiting the group of multi-trait strains to eight. The selected bacterial strains were further evaluated for PGP attributes associated with biofilm formation, compatibility, salt tolerance ability and effect on plant growth. In vitro studies demonstrated that the multi-trait isolates, Bacillus (1.SG.7, 5.SG.3) and Pseudomonas (2.SG.20, 2.C.19) strains, enhanced the lateral roots abundance and shoots biomass, mitigated salinity stress, suggesting the utility of beneficial ARS-producing bacteria as potential microbial fertilizers. Furthermore, in vitro studies demonstrated that compatible combinations of multi-trait isolates, Bacillus sp. 1.SG.7 in a mixture coupled with 5.SG.3, and 2.C.19 with 5.SG.3 belonging to Bacillus and Pseudomonas, respectively, may enhance plant growth as compared to single inoculants.

Sign in / Sign up

Export Citation Format

Share Document