Synthesis of Poly(Dimethylmalic Acid) Homo- and Copolymers to Produce Biodegradable Nanoparticles for Drug Delivery: Cell Uptake and Biocompatibility Evaluation in Human Heparg Hepatoma Cells

Hydrophobic and amphiphilic derivatives of the biocompatible and biodegradable poly(dimethylmalic acid) (PdiMeMLA), varying by the nature of the lateral chains and the length of each block, respectively, have been synthesized by anionic ring-opening polymerization (aROP) of the corresponding monomers using an initiator/base system, which allowed for very good control over the (co)polymers’ characteristics (molar masses, dispersity, nature of end-chains). Hydrophobic and core-shell nanoparticles (NPs) were then prepared by nanoprecipitation of hydrophobic homopolymers and amphiphilic block copolymers, respectively. Negatively charged NPs, showing hydrodynamic diameters (Dh) between 50 and 130 nm and narrow size distributions (0.08 < PDI < 0.22) depending on the (co)polymers nature, were obtained and characterized by dynamic light scattering (DLS), zetametry, and transmission electron microscopy (TEM). Finally, the cytotoxicity and cellular uptake of the obtained NPs were evaluated in vitro using the hepatoma HepaRG cell line. Our results showed that both cytotoxicity and cellular uptake were influenced by the nature of the (co)polymer constituting the NPs.

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Variably Sized and Multi-Colored Silica-Nanoparticles Characterized by Fluorescence Correlation Methods for Cellular Dynamics

Materials ◽

10.3390/ma14010019 ◽

2020 ◽

Vol 14 (1) ◽

pp. 19

Author(s):

Chan-Gi Pack ◽

Bjorn Paulson ◽

Yeonhee Shin ◽

Min Kyo Jung ◽

Jun Sung Kim ◽

...

Keyword(s):

Electron Microscopy ◽

Correlation Spectroscopy ◽

Cell Uptake ◽

Core Shell ◽

Therapeutic Effects ◽

Ferrite Core ◽

Shell Nanoparticles ◽

Fluorescence Correlation ◽

Correlation Methods

Controlling the uptake of nanoparticles into cells so as to balance therapeutic effects with toxicity is an essential unsolved problem in the development of nanomedicine technologies. From this point of view, it is useful to use standard nanoparticles to quantitatively evaluate the physical properties of the nanoparticles in solution and in cells, and to analyze the intracellular dynamic motion and distribution of these nanoparticles at a single-particle level. In this study, standard nanoparticles are developed based on a variant silica-based nanoparticle incorporating fluorescein isothiocyanate (FITC) or/and rhodamine B isothiocyanate (RITC) with a variety of accessible diameters and a matching fluorescent cobalt ferrite core-shell structure (Fe2O4/SiO2). The physical and optical properties of the nanoparticles in vitro are fully evaluated with the complementary methods of dynamic light scattering, electron microscopy, and two fluorescence correlation methods. In addition, cell uptake of dual-colored and core/shell nanoparticles via endocytosis in live HeLa cells is detected by fluorescence correlation spectroscopy and electron microscopy, indicating the suitability of the nanoparticles as standards for further studies of intracellular dynamics with multi-modal methods.

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Study of photodynamic activity of Au@SiO2 core-shell nanoparticles in vitro

Materials Science and Engineering C ◽

10.1016/j.msec.2016.03.010 ◽

2016 ◽

Vol 63 ◽

pp. 317-322 ◽

Cited By ~ 9

Author(s):

K.S. Meena ◽

K.I. Dhanalekshmi ◽

K. Jayamoorthy

Keyword(s):

Core Shell ◽

Shell Nanoparticles ◽

Photodynamic Activity ◽

Core Shell Nanoparticles

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Co-delivery of curcumin and piperine in zein-carrageenan core-shell nanoparticles: Formation, structure, stability and in vitro gastrointestinal digestion

Food Hydrocolloids ◽

10.1016/j.foodhyd.2019.105334 ◽

2020 ◽

Vol 99 ◽

pp. 105334 ◽

Cited By ~ 25

Author(s):

Shuai Chen ◽

Qike Li ◽

David Julian McClements ◽

Yahong Han ◽

Lei Dai ◽

...

Keyword(s):

Structure Stability ◽

Core Shell ◽

Gastrointestinal Digestion ◽

Shell Nanoparticles ◽

In Vitro Gastrointestinal Digestion ◽

Core Shell Nanoparticles

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Remarkably enhanced stability and function of core/shell nanoparticles composed of a lecithin core and a pluronic shell layer by photo-crosslinking the shell layer: In vitro and in vivo study

Acta Biomaterialia ◽

10.1016/j.actbio.2010.01.029 ◽

2010 ◽

Vol 6 (7) ◽

pp. 2666-2673 ◽

Cited By ~ 20

Author(s):

Won Il Choi ◽

Kyung Chul Yoon ◽

Seong Kyu Im ◽

Young Ha Kim ◽

Soon Hong Yuk ◽

...

Keyword(s):

In Vivo Study ◽

Core Shell ◽

Shell Layer ◽

Shell Nanoparticles ◽

And Function ◽

Core Shell Nanoparticles ◽

Enhanced Stability

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PEG-Iron Oxide Core-Shell Nanoparticles: In situ Synthesis and In vitro Biocompatibility Evaluation for Potential T2-MRI Applications

BioNanoScience ◽

10.1007/s12668-020-00791-5 ◽

2020 ◽

Vol 10 (4) ◽

pp. 1107-1120

Author(s):

Karina Almeida Barcelos ◽

Marli Luiza Tebaldi ◽

Eryvaldo Socrates Tabosa do Egito ◽

Nádia Miriceia Leão ◽

Daniel Cristian Ferreira Soares

Keyword(s):

Iron Oxide ◽

In Situ Synthesis ◽

Core Shell ◽

Shell Nanoparticles ◽

In Vitro Biocompatibility ◽

Iron Oxide Core ◽

Core Shell Nanoparticles ◽

T2 Mri

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Development of NIPAAm-PEG acrylate polymeric nanoparticles for co-delivery of paclitaxel with ellagic acid for the treatment of breast cancer

Journal of Polymer Engineering ◽

10.1515/polyeng-2018-0169 ◽

2019 ◽

Vol 39 (3) ◽

pp. 271-278 ◽

Cited By ~ 3

Author(s):

Suruchi Suri ◽

Mohd. Aamir Mirza ◽

Md. Khalid Anwer ◽

Abdullah S. Alshetaili ◽

Saad M. Alshahrani ◽

...

Keyword(s):

Breast Cancer ◽

Particle Size ◽

Ellagic Acid ◽

Oral Delivery ◽

Polymeric Nanoparticles ◽

Entrapment Efficiency ◽

Shell Nanoparticles ◽

Transmission Electron ◽

Core Shell Nanoparticles

Abstract The aim of the current study was to develop a dual-loaded core shell nanoparticles encapsulating paclitaxel (PTX) and ellagic acid (EA) by membrane dialysis method. Based on particle size, polydispersity index (PDI), and entrapment efficiency, the dual drug-loaded nanoparticles (F2) was optimized. The optimized nanoparticles (F2) showed a particle size of 140±2 nm and a PDI of 0.23±3. The size and the morphology were confirmed by transmission electron microscopy (TEM) and found agreement with the results of dynamic light scattering. The entrapment efficiencies of total drug (PTX and EA), PTX, and EA in the nanoparticles (F2) were measured as 80%, 62.3%, and 37.7%, respectively. The in vitro release profile showed a controlled release pattern for 48 h. A higher cytotoxicity was observed with nanoparticles (F2) in comparison to free PTX. The results revealed that co-delivery of PTX and EA could be used for its oral delivery for the effective treatment of breast cancer.

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Albumin-Albumin/Lactosylated Core-Shell Nanoparticles: Therapy to Treat Hepatocellular Carcinoma for Controlled Delivery of Doxorubicin

Molecules ◽

10.3390/molecules25225432 ◽

2020 ◽

Vol 25 (22) ◽

pp. 5432

Author(s):

Nayelli Guadalupe Teran-Saavedra ◽

Jose Andrei Sarabia-Sainz ◽

Enrique Fernando Velázquez-Contreras ◽

Gabriela Ramos-Clamont Montfort ◽

Martín Pedroza-Montero ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Side Effects ◽

Hepg2 Cells ◽

Core Shell ◽

Shell Nanoparticles ◽

Human Liver Cancer ◽

Systemic Side Effects ◽

Human Liver Cancer Cells ◽

Core Shell Nanoparticles

Doxorubicin (Dox) is the most widely used chemotherapeutic agent and is considered a highly powerful and broad-spectrum for cancer treatment. However, its application is compromised by the cumulative side effect of dose-dependent cardiotoxicity. Because of this, targeted drug delivery systems (DDS) are currently being explored in an attempt to reduce Dox systemic side-effects. In this study, DDS targeting hepatocellular carcinoma (HCC) has been designed, specifically to the asialoglycoprotein receptor (ASGPR). Dox-loaded albumin-albumin/lactosylated (core-shell) nanoparticles (tBSA/BSALac NPs) with low (LC) and high (HC) crosslink using glutaraldehyde were synthesized. Nanoparticles presented spherical shapes with a size distribution of 257 ± 14 nm and 254 ± 14 nm, as well as an estimated surface charge of −28.0 ± 0.1 mV and −26.0 ± 0.2 mV, respectively. The encapsulation efficiency of Dox for the two types of nanoparticles was higher than 80%. The in vitro drug release results showed a sustained and controlled release profile. Additionally, the nanoparticles were revealed to be biocompatible with red blood cells (RBCs) and human liver cancer cells (HepG2 cells). In cytotoxicity assays, Dox-loaded nanoparticles decrease cell viability more efficiently than free Dox. Specific biorecognition assays confirmed the interaction between nanoparticles and HepG2 cells, especially with ASGPRs. Both types of nanoparticles may be possible DDS specifically targeting HCC, thus reducing side effects, mainly cardiotoxicity. Therefore, improving the quality of life from patients during chemotherapy.

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