Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection

Prosthetic mesh infection is a devastating complication of abdominal hernia repair which impairs natural healing in the implant area, leading to increased rates of patient morbidity, mortality, and prolonged hospitalization. This preclinical study was designed to assess the effects on abdominal wall tissue repair of coating meshes with a chlorhexidine or rifampicin-carboxymethylcellulose biopolymer gel in a Staphylococcus aureus (S. aureus) infection model. Partial abdominal wall defects were created in New Zealand white rabbits (n = 20). Four study groups were established according to whether the meshes were coated or not with each of the antibacterial gels. Three groups were inoculated with S. aureus and finally repaired with lightweight polypropylene mesh. Fourteen days after surgery, implanted meshes were recovered for analysis of the gene and protein expression of collagens, macrophage phenotypes, and mRNA expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Compared to uncoated meshes, those coated with either biopolymer gel showed higher collagen 1/3 messenger RNA and collagen I protein expression, relatively increased VEGF mRNA expression, a significantly reduced macrophage response, and lower relative amounts of MMPs mRNAs. Our findings suggest that following mesh implant these coatings may help improving abdominal wall tissue repair in the presence of infection.

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Self-adhesive hydrogel meshes reduce tissue incorporation and mechanical behavior versus microgrips self-fixation: a preclinical study

Hernia ◽

10.1007/s10029-021-02552-z ◽

2022 ◽

Author(s):

Selma Benito-Martínez ◽

Marta Rodríguez ◽

Francisca García-Moreno ◽

Bárbara Pérez-Köhler ◽

Estefanía Peña ◽

...

Keyword(s):

Hernia Repair ◽

Mechanical Behavior ◽

Mesh Fixation ◽

Host Tissue ◽

Tissue Adhesive ◽

Abdominal Hernia ◽

Abdominal Wall Defects ◽

Macrophage Response ◽

Abdominal Hernia Repair ◽

Tissue Incorporation

Abstract Purpose Atraumatic mesh fixation for abdominal hernia repair has been developed to avoid the disadvantages of classical fixation with sutures, which is considered a cause of chronic pain and discomfort. This study was designed to analyze, in the short and medium term, the biological and mechanical behavior of two self-fixing meshes compared to that of a polypropylene (PP) mesh fixed with a cyanoacrylate (CA) tissue adhesive. Methods Partial abdominal wall defects (6 × 4 cm) were created in New Zealand rabbits (n = 36) and repaired using a self-adhesive hydrogel mesh (Adhesix™), a self-gripping mesh (ProGrip™) or a PP mesh fixed with CA (Surgipro™ CA). After 14 and 90 days, the host tissue incorporation, macrophage response and biomechanical strength were examined. Results At 14 and 90 days, the ProGrip and Surgipro CA meshes showed good host tissue incorporation; however, the Adhesix implants presented poor integration, seroma formation and a higher degree of shrinkage. The Adhesix hydrogel was completely reabsorbed at 14 days, whereas ProGrip microhooks were observed at all study times. The macrophage response was higher in the ProGrip and Surgipro CA groups at 14 and 90 days, respectively, and decreased over time. At 90 days, the ProGrip implants showed the highest tensile strength values and the Adhesix implants showed the highest failure stretch. Conclusion Meshes with mechanical microgrip self-fixation (ProGrip) show better biological and mechanical behavior than those with adhesive hydrogel (Adhesix) in a preclinical model of abdominal hernia repair in rabbits.

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Identification of pluripotent cells in bovine uterus: in situ and in vitro studies

Reproduction ◽

10.1530/rep-14-0348 ◽

2015 ◽

Vol 149 (4) ◽

pp. 317-327 ◽

Cited By ~ 14

Author(s):

Martyna Łupicka ◽

Gabriel Bodek ◽

Nahum Shpigel ◽

Ehud Elnekave ◽

Anna J Korzekwa

Keyword(s):

Flow Cytometry ◽

Protein Expression ◽

Mrna Expression ◽

Uterine Horn ◽

Uterine Tissue ◽

Pluripotent Cells ◽

Flow Cytometry Assay ◽

Myometrial Cells ◽

Gene And Protein Expression

The aim of this study was to identify uterine pluripotent cells both in bovine uterine tissues as well in epithelial, stromal, and myometrial uterine cell populations. Moreover, the relationship of pluripotent markers expression with age and the uterine horn side was considered. Uterine tissue was collected from ipsilateral and contralateral horns (days 8–10 of the estrous cycle). Immunohistostaining for C-KIT, OCT3/4, NANOG, and SOX2 in uterine tissue was determined. mRNA expression of C-KIT, OCT3/4, NANOG and SOX2 was evaluated in uterine tissue relative to the age of the cow and uterine horn side. Gene and protein expression of these markers in the uterine luminal epithelial, stromal, and myometrial cells was evaluated by real-time PCR and western blotting respectively. The expression of pluripotent cell markers OCT3/4, NANOG, and SOX2 was identified by flow cytometry assay in epithelial, stromal, and myometrial cells. Multilineage differentiation of the bovine uterine cells was performed. mRNA expression of OCT3/4, NANOG, and SOX2 in uterine tissue was higher in the ipsilateral horn than in the contralateral horn. Flow cytometry assay revealed positive fluorescence for OCT3/4, NANOG, and SOX2 in all uterine cell types. Results showed the age-dependent expression of pluripotent markers in uterine tissue. Beside, the different expression of pluripotent cells in each horn of uterus suggests the influence of ovarian hormones on these characteristics. The highest mRNA and protein expression for pluripotent markers was observed in stromal cells among uterine cells, which indicates this population of cells as the main site of pluripotent cells in the cow uterus.

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Ventral Abdominal Hernia Repair: MIS Extraperitoneal Repair Techniques: eTEP Rives, MILOS/EMILOS, and Onlay MIS Repair

The SAGES Manual of Hernia Surgery ◽

10.1007/978-3-319-78411-3_20 ◽

2018 ◽

pp. 271-290 ◽

Cited By ~ 2

Author(s):

Flavio Malcher Martins de Oliveira ◽

Leandro Totti Cavazzola ◽

Adam S. Weltz ◽

Igor Belyansky

Keyword(s):

Hernia Repair ◽

Abdominal Hernia ◽

Abdominal Hernia Repair ◽

Ventral Abdominal Hernia ◽

Repair Techniques

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Pre-clinical assay of the tissue integration and mechanical adhesion of several types of cyanoacrylate adhesives in the fixation of lightweight polypropylene meshes for abdominal hernia repair

PLoS ONE ◽

10.1371/journal.pone.0206515 ◽

2018 ◽

Vol 13 (11) ◽

pp. e0206515 ◽

Cited By ~ 1

Author(s):

Gemma Pascual ◽

Claudia Mesa-Ciller ◽

Marta Rodríguez ◽

Bárbara Pérez-Köhler ◽

Verónica Gómez-Gil ◽

...

Keyword(s):

Hernia Repair ◽

Abdominal Hernia ◽

Tissue Integration ◽

Clinical Assay ◽

Abdominal Hernia Repair ◽

Mechanical Adhesion

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Prospective Case Series Evaluating a Large Fenestrated Bovine Acellular Dermal Matrix in Patients Undergoing Complex Abdominal Hernia Repair

Journal of the American College of Surgeons ◽

10.1016/j.jamcollsurg.2020.08.355 ◽

2020 ◽

Vol 231 (4) ◽

pp. e135

Author(s):

Ricardo Alejandro Fonseca ◽

Jose Alejandro Aldana ◽

Melissa Canas ◽

Christina Xinyue Zhang ◽

James Michael McMullen ◽

...

Keyword(s):

Hernia Repair ◽

Case Series ◽

Acellular Dermal Matrix ◽

Abdominal Hernia ◽

Dermal Matrix ◽

Abdominal Hernia Repair ◽

Prospective Case Series ◽

Acellular Dermal

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Structural Characterization and Antioxidant Activity of Polysaccharides from Athyrium multidentatum (Doll.) Ching in d-Galactose-Induced Aging Mice via PI3K/AKT Pathway

Molecules ◽

10.3390/molecules24183364 ◽

2019 ◽

Vol 24 (18) ◽

pp. 3364 ◽

Cited By ~ 2

Author(s):

Liang Jing ◽

Jing-Ru Jiang ◽

Dong-Mei Liu ◽

Ji-Wen Sheng ◽

Wei-Fen Zhang ◽

...

Keyword(s):

Oxidative Stress ◽

Molecular Weight ◽

Protein Expression ◽

Mrna Expression ◽

Caspase 3 ◽

Akt Pathway ◽

Protective Mechanism ◽

Related Factor ◽

Bax Protein ◽

Gene And Protein Expression

The purpose of this study was to characterize the polysaccharides from Athyrium multidentatum (Doll.) Ching (AMC) rhizome and explore the protective mechanism against d-galactose-induced oxidative stress in aging mice. Methods: A series of experiments, including molecular weight, monosaccharide composition, Fourier transform infrared (FT-IR) spectroscopy, and 1H nuclear magnetic resonance (1H NMR) spectroscopy were carried out to characterize AMC polysaccharides. The mechanism was investigated exploring d-galactose-induced aging mouse model. Quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) and western blotting assays were performed to assess the gene and protein expression in liver. Key findings: Our results showed that AMC polysaccharides were mainly composed of mannose (Man), rhamnose (Rha), glucuronic acid (Glc A), glucose (Glc), galactose (Gal), arabinose (Ara), and fucose (Fuc) in a molar ratio of 0.077:0.088:0.09:1:0.375:0.354:0.04 with a molecular weight of 33203 Da (Mw). AMC polysaccharides strikingly reversed d-galactose-induced changes in mice, including upregulated phosphatidylinositol 3-kinase (PI3K), Akt, nuclear factor-erythroid 2-related factor 2 (Nrf2), forkhead box O3a (FOXO3a), and hemeoxygenase-1 (HO-1) mRNA expression, raised Bcl-2/Bax ratio, downregulated caspase-3 mRNA expression, enhanced Akt, phosphorylation of Akt (p-Akt), Nrf2 and HO-1 protein expression, decreased caspase-3, and Bax protein expression. Conclusion: AMC polysaccharides attenuated d-galactose-induced oxidative stress and cell apoptosis by activating the PI3K/AKT pathway, which might in part contributed to their anti-aging activity.

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Outcome of Abdominal Wall Hernia Repair with Biologic Mesh: Permacol™ versus Strattice™

The American Surgeon ◽

10.1177/000313481408001019 ◽

2014 ◽

Vol 80 (10) ◽

pp. 999-1002 ◽

Cited By ~ 21

Author(s):

Amy W. Cheng ◽

Maher A. Abbas ◽

Talar Tejirian

Keyword(s):

Hernia Repair ◽

Abdominal Wall ◽

Abdominal Wall Hernia ◽

Hernia Recurrence ◽

Biologic Mesh ◽

Abdominal Hernia ◽

Complication Rates ◽

Short Term ◽

Porcine Dermis ◽

Abdominal Wall Hernia Repair

The use of biologic mesh in abdominal wall operations has gained popularity despite a paucity of outcome data. Numerous biologic products are available with virtually no clinical comparison studies. A retrospective study was conducted to compare patients who underwent abdominal wall hernia repair with Permacol™ (crosslinked porcine dermis) and Strattice™ (noncrosslinked porcine dermis). Of 270 reviewed patients, 195 were implanted with Permacol™ and 75 with Strattice™. Ventral hernia repairs comprised the majority (85% for Permacol, 97% for Strattice™). Postoperative infection rate was lower in the Strattice™ group (5 vs 21%, P < 0.01). In the Permacol™ group only, the overall complication rates were significantly higher in patients with infected versus clean wounds (55 vs 35%, P < 0.05) and in obese patients (body mass index 40 kg/m2 or greater [57 vs 34%], P < 0.01). Short-term complication and recurrence rates were higher when mesh was used as a fascial bridge: 51 versus 28 per cent for Permacol™, 58 versus 20 per cent for Strattice™. The hernia recurrence was similar in both groups. In this review of patients undergoing abdominal hernia repair with biologic mesh, Strattice™ mesh was associated with a lower short-term complication rate compared with Permacol™, but the hernia recurrence rate was similar.

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