The Bacterial Control of Poly (Lactic Acid) Nanofibers Loaded with Plant-Derived Monoterpenoids via Emulsion Electrospinning

Plant-derived monoterpenoids have been shown to possess various biological effects, providing a scientific basis for their potential usage as antibacterial agents. Therefore, considering problems surrounding bacteria′s antibacterial resistance, the utilization of natural antimicrobial compounds such as monoterpenoids in different industries has gained much attention. The aim of this study was to fabricate and characterize various concentrations of plant-derived monoterpenoids, geraniol (G) and carvacrol (C), loaded into poly(lactic acid) (PLA) nanofibers via emulsion electrospinning. The antibacterial activities of the fabricated nanofibers were evaluated using three types of antibacterial assays (inhibition zone tests, live/dead bacterial cell assays, and antibacterial kinetic growth assays). Among the samples, 10 wt% carvacrol-loaded PLA nanofibers (C10) had the most bactericidal activity, with the widest inhibition zone of 5.26 cm and the highest visible dead bacteria using the inhibition zone test and live/dead bacterial cell assay. In order to quantitatively analyze the antibacterial activities of 5 wt% carvacrol-loaded PLA nanofibers (C5), C10, 5 wt% geraniol-loaded PLA nanofibers (G5), and 10 wt% geraniol-loaded PLA nanofibers (G10) against E. coli and S.epidermidis, growth kinetic curves were analyzed using OD600. For the results, we found that the antibacterial performance was as follows: C10 > C5 > G10 > G5. Overall, carvacrol or geraniol-loaded PLA nanofibers are promising antibacterial materials for improving fiber functionality.

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Antibacterial Performance of Chitin Nanowhisker Reinforced Poly(lactic acid) Composite Nanofiber Membrane

Advanced Science Letters ◽

10.1166/asl.2012.3392 ◽

2012 ◽

Vol 10 (1) ◽

pp. 649-651 ◽

Cited By ~ 3

Author(s):

Jing Wei ◽

Jingyan Liu ◽

Jing Qiang ◽

Lina Yang ◽

Yuqin Wan ◽

...

Keyword(s):

Lactic Acid ◽

Poly Lactic Acid ◽

Nanofiber Membrane ◽

Antibacterial Performance ◽

Composite Nanofiber

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Flexible fibrous membrane based on copper(II) metal-organic framework/poly(lactic acid) composites with superior antibacterial performance

Biomaterials Science ◽

10.1039/d1bm00164g ◽

2021 ◽

Author(s):

Zhao Liu ◽

Junwei Ye ◽

Abdul Rauf ◽

Siqi Zhang ◽

Guangyao Wang ◽

...

Keyword(s):

Lactic Acid ◽

Metal Organic Framework ◽

Three Dimensional ◽

Fibrous Membrane ◽

Poly Lactic Acid ◽

Healthcare Applications ◽

Antibacterial Efficiency ◽

Antibacterial Performance ◽

Metal Organic ◽

Organic Framework

Flexible antibacterial fibrous membrane employing high antibacterial efficiency has great potential in healthcare applications. Herein, a three-dimensional copper(II) metal-organic framework [Cu2(CA)(H2O)2, Cu-MOF-1] and poly(lactic acid) (PLA) composite fibrous membrane was...

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Controlled Release of Thymol from Poly(Lactic Acid)-Based Silver Nanocomposite Films with Antibacterial and Antioxidant Activity

Antioxidants ◽

10.3390/antiox9050395 ◽

2020 ◽

Vol 9 (5) ◽

pp. 395 ◽

Cited By ~ 3

Author(s):

Marina Ramos ◽

Ana Beltran ◽

Elena Fortunati ◽

Mercedes Peltzer ◽

Francesco Cristofaro ◽

...

Keyword(s):

Lactic Acid ◽

Controlled Release ◽

Nanocomposite Films ◽

Poly Lactic Acid ◽

Spectroscopic Techniques ◽

Ag Nps ◽

Antibacterial Performance ◽

Dpph Method ◽

Potential Applications ◽

Silver Nanocomposite

Thymol and silver nanoparticles (Ag-NPs) were used to develop poly(lactic acid) (PLA)-based films with antioxidant and antibacterial performance. Different amounts of thymol (6 and 8 wt%) and 1 wt% Ag-NPs were added to PLA to produce the active films. Ag-NPs and thymol were successfully identified in the nanocomposite structures using spectroscopic techniques. A kinetic study was performed to evaluate the release of thymol and Ag-NPs from the nanocomposites to an aqueous food simulant (ethanol 10%, v/v) at 40 °C. The diffusion of thymol from the polymer matrix was affected by the presence of non-migrating Ag-NPs, which showed non-Fickian release behavior. The ternary system including 1 wt% Ag-NPs and 8 wt% thymol showed clear antibacterial performance by reducing the cell viability of Escherichia coli and Staphylococcus aureus by around 40% after 3 and 24 h of storage at 4, 25, and 37 °C compared to neat PLA. Significant antioxidant behavior of all active films was also confirmed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method. The obtained nanocomposite films based on PLA and the addition of Ag-NPs and thymol were proven to have combined antioxidant and antibacterial performance, with controlled release of thymol. These formulations have potential applications in the development of innovative and customized active packaging systems to increase the shelf-life of food products.

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Chemical Composition and Antibacterial Activity of Essential Oils ofTagetes minuta(Asteraceae) against Selected Plant Pathogenic Bacteria

International Journal of Microbiology ◽

10.1155/2016/7352509 ◽

2016 ◽

Vol 2016 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Martin Muthee Gakuubi ◽

John M. Wagacha ◽

Saifuddin F. Dossaji ◽

Wycliffe Wanzala

Keyword(s):

Antibacterial Activity ◽

Chemical Composition ◽

Essential Oils ◽

Pathogenic Bacteria ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Scientific Basis ◽

Diffusion Method ◽

Phytopathogenic Bacteria ◽

Xanthomonas Axonopodis

The objective of this study was to determine the chemical composition and antibacterial activity of essential oils (EOs) ofTagetes minutaagainst three phytopathogenic bacteriaPseudomonas savastanoipv.phaseolicola,Xanthomonas axonopodispv.phaseoli, andXanthomonas axonopodispv.manihotis. The essential oils were extracted using steam distillation method in a modified Clevenger-type apparatus while antibacterial activity of the EOs was evaluated by disc diffusion method. Gas chromatography coupled to mass spectrometry (GC/MS) was used for analysis of the chemical profile of the EOs. Twenty compounds corresponding to 96% of the total essential oils were identified with 70% and 30% of the identified components being monoterpenes and sesquiterpenes, respectively. The essential oils ofT. minutarevealed promising antibacterial activities against the test pathogens withPseudomonas savastanoipv.phaseolicolabeing the most susceptible with mean inhibition zone diameters of 41.83 and 44.83 mm after 24 and 48 hours, respectively.The minimum inhibitory concentrations and minimum bactericidal concentrations of the EOs on the test bacteria were in the ranges of 24–48 mg/mL and 95–190 mg/mL, respectively. These findings provide a scientific basis for the use ofT. minutaessential oils as a botanical pesticide for management of phytopathogenic bacteria.

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Preparation of cypress (Cupressus sempervirens L.) essential oil loaded poly(lactic acid) nanofibers

Open Chemistry ◽

10.1515/chem-2021-0065 ◽

2021 ◽

Vol 19 (1) ◽

pp. 796-805

Author(s):

Hayfa Argui ◽

Salih Can Suner ◽

Çağdaş Deniz Periz ◽

Seyhan Ulusoy ◽

Gülen Türker ◽

...

Keyword(s):

Lactic Acid ◽

Essential Oil ◽

Inhibition Zone ◽

Mechanical Analysis ◽

Polymer Matrices ◽

Poly Lactic Acid ◽

Mechanical And Thermal Properties ◽

Cupressus Sempervirens ◽

E Coli ◽

Thermal Stability Of

Abstract This study investigated the production of poly(lactic acid) (PLA) nanofibers containing cypress (CUP) essential oil (EO) via electrospinning. The nanofibers were produced from polymer solution prepared with different percentages of cypress EO. Cypress EO-containing PLA nanofibers were characterized and some mechanical and thermal properties were examined using thermogravimetric analysis, scanning electron microscopy, Fourier-transform infrared spectroscopy, and dynamic mechanical analysis. The thermal stability of the nanofibers was reduced depending on the percentage of the cypress EO. As the ratio of the cypress EO to polymer matrices was increased, it was observed that the glassy transition temperatures of the nanofibers decreased and their flexibility increased. The T g value was determined to be 53.74°C for the neat PLA nanofiber, while 51.83°C for the PLA-CUP nanofiber (containing 15% cypress EO). According to the results of releasing trial, the increased amount of cypress EO resulted in less cypress EO releasing from polymer matrices. The nanofibers were observed to exhibit antibacterial activity against Escherichia coli and Staphylococcus aureus. The inhibition zone diameter of the nanofibers containing 10% cypress EO was 20 mm for S. aureus and 16 mm for E. coli, while 10 mm in the presence of Kanamycin.

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Geraniol and cinnamaldehyde as natural antibacterial additives for poly(lactic acid) and their plasticizing effects

Journal of Polymer Engineering ◽

10.1515/polyeng-2019-0198 ◽

2019 ◽

Vol 40 (1) ◽

pp. 38-48

Author(s):

Mert Akgün ◽

İhsan Başaran ◽

Salih C. Suner ◽

Ayhan Oral

Keyword(s):

Lactic Acid ◽

Antibacterial Activities ◽

Mechanical Tests ◽

Sem Analysis ◽

Diffusion Method ◽

Poly Lactic Acid ◽

Scanning Calorimetry ◽

Disk Diffusion Method ◽

Vis Spectroscopy ◽

The Matrix

Abstract The main goal of this study is to prepare antibacterial poly(lactic acid) (PLA) containing cinnamaldehyde and geraniol and to evaluate the antibacterial activity and assess the changes of physical properties of the PLA films. Cinnamaldehyde- and geraniol-incorporated (10%, 20%, 30%, and 50% v/w) PLA films were prepared via solution-casting. While preparing these films, plasticizers were not added to the matrix. Antibacterial activities of these films against Escherichia coli and Staphylococcus aureus were investigated by the disk diffusion method. Thermal degradation characteristics were analyzed via thermogravimetric analysis (TGA), glass transition, crystallization, and melting temperatures, and enthalpies of the films were determined from differential scanning calorimetry (DSC) scans. Tensile strength and elongation-at-break values of neat PLA and antibacterial-compound-containing films were evaluated and compared after the mechanical tests. Moreover, the changes in the polymer morphology were observed by SEM analysis, and opacity of the films was determined by UV-vis spectroscopy. Our results showed that both compounds provided antibacterial effect to the PLA, with cinnamaldehyde being more effective than geraniol. Moreover, plasticization effects of the compounds were confirmed by DSC analysis.

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Reducing cytotoxicity of poly (lactic acid)-based/zinc oxide nanocomposites while boosting their antibacterial activities by thymol for biomedical applications

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2020.09.069 ◽

2020 ◽

Vol 164 ◽

pp. 4556-4565

Author(s):

Sina Kazemi-Pasarvi ◽

Nadereh Golshan Ebrahimi ◽

Dina Shahrampour ◽

Zahra Arab-Bafrani

Keyword(s):

Zinc Oxide ◽

Lactic Acid ◽

Biomedical Applications ◽

Antibacterial Activities ◽

Poly Lactic Acid

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Antidiabetic, Antioxidants and Antibacterial Activities of Lactic Acid Bacteria (LAB) from Masin (Fermented Sauce from Sumbawa, West Nusa Tenggara, Indonesia)

ANNALES BOGORIENSES ◽

10.14203/ann.bogor.2020.v24.n1.27-34 ◽

2020 ◽

Vol 24 (1) ◽

pp. 27

Author(s):

Baso Manguntungi ◽

Dinar Suksmayu Saputri ◽

Apon Zaenal Mustopa ◽

Nurlaili Ekawati ◽

Maritsa Nurfatwa ◽

...

Keyword(s):

Antioxidant Activity ◽

Antibacterial Activity ◽

Lactic Acid ◽

Lactic Acid Bacteria ◽

Inhibition Zone ◽

Antibacterial Activities ◽

Antidiabetic Activity ◽

Strain Atcc ◽

Antibacterial Compounds ◽

Effective Antioxidant

The study aimed to determine the effectiveness of metabolites from Lactic Acid Bacteria (LAB) derived Masin (fermented sauce from Sumbawa) as antioxidant, antidiabetic, and antibacterial compounds. The LAB isolates were isolated from various strains of Staphylococcus piscifermentan which consisted of Staphylococcus piscifermentans strain CIP103958 (code: 2), strain BULST54 (code: 17), strain SK03 (code: 11), strain ATCC 51136 (code: 34), strain PCM 2409 (code: 28) and strain PU-87 (code: 5). The Metabolites of LAB were analyzed by the bioprospecting test to indicate antidiabetic, antioxidant and antibacterial activities. The isolate (Code: 5) at 500 ug/ml showed the most effective antioxidant activity up to 71%. The isolate (code: 28), at 300 ug/ml revealed to have the most antidiabetic activity up to 43 %. The isolate (code: 2) showed moderate antibacterial activity with the inhibition zone of 5.59 mm. The results of the antidiabetic, antioxidant and antibacterial activity showed that the secondary metabolites produced by LAB from the Masin have broad activities as an antidiabetic, antioxidant and antibacterial.

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