scholarly journals Upcycling Biodegradable PVA/Starch Film to a Bacterial Biopigment and Biopolymer

Polymers ◽  
2021 ◽  
Vol 13 (21) ◽  
pp. 3692
Author(s):  
Brana Pantelic ◽  
Marijana Ponjavic ◽  
Vukasin Jankovic ◽  
Ivana Aleksic ◽  
Sanja Stevanovic ◽  
...  
Keyword(s):  
Food Packaging ◽  
Ralstonia Eutropha ◽  
Dry Weight ◽  
Thermoplastic Starch ◽  
Low Carbon ◽  
Bacterial Strains ◽  
Streptomyces Sp ◽  
Substrate Conversion ◽  
Ralstonia Eutropha H16 ◽  
Film Substrate

Meeting the challenge of circularity for plastics requires amenability to repurposing post-use, as equivalent or upcycled products. In a compelling advancement, complete circularity for a biodegradable polyvinyl alcohol/thermoplastic starch (PVA/TPS) food packaging film was demonstrated by bioconversion to high-market-value biopigments and polyhydroxybutyrate (PHB) polyesters. The PVA/TPS film mechanical properties (tensile strength (σu), 22.2 ± 4.3 MPa; strain at break (εu), 325 ± 73%; and Young’s modulus (E), 53–250 MPa) compared closely with low-density polyethylene (LDPE) grades used for food packaging. Strong solubility of the PVA/TPS film in water was a pertinent feature, facilitating suitability as a carbon source for bioprocessing and microbial degradation. Biodegradability of the film with greater than 50% weight loss occurred within 30 days of incubation at 37 °C in a model compost. Up to 22% of the PVA/TPS film substrate conversion to biomass was achieved using three bacterial strains, Ralstonia eutropha H16 (Cupriavidus necator ATCC 17699), Streptomyces sp. JS520, and Bacillus subtilis ATCC6633. For the first time, production of the valuable biopigment (undecylprodigiosin) by Streptomyces sp. JS520 of 5.3 mg/mL and the production of PHB biopolymer at 7.8% of cell dry weight by Ralstonia eutropha H16 from this substrate were reported. This low-energy, low-carbon post-use PVA/TPS film upcycling model approach to plastic circularity demonstrates marked progress in the quest for sustainable and circular plastic solutions.

CbbR and RegA regulate cbb operon transcription in Ralstonia eutropha H16

2017 ◽  
Vol 257 ◽  
pp. 78-86 ◽  
Author(s):  
Steffen Gruber ◽  
Helmut Schwab ◽  
Petra Heidinger
Keyword(s):  
Ralstonia Eutropha ◽  
Ralstonia Eutropha H16

scholarly journals New Circular Challenges in the Development of Take-Away Food Packaging in the COVID-19 Period

Energies ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4705
Author(s):  
Ewa Kochańska ◽  
Rafał M. Łukasik ◽  
Maciej Dzikuć
Keyword(s):  
Social Transformation ◽  
Food Packaging ◽  
New Technologies ◽  
Negative Impact ◽  
Raw Materials ◽  
Distribution Channels ◽  
Low Carbon ◽  
Industrial Transformation ◽  
Specific Distribution ◽  
Food Market

The COVID-19 pandemic has set new challenges for the HoReCa industry. Lockdowns have coincided with and strongly impacted the industrial transformation processes that have been taking place for a decade. Among the most important HoReCa transition processes are those related to the rapid growth of the delivery-food market and ordering meals via internet platforms. The new delivery-food market requires not only the development of specific distribution channels, but also the introduction of appropriate, very specific food packaging. Food packaging and its functionality are defined by the administrative requirements and standards applicable to materials that have contact with food and principally through the prism of the ecological disaster caused by enormous amounts of plastic waste, mainly attributed to the food packaging. To meet environmental and administrative requirements, new technologies to produce food packaging materials are emerging, ensuring product functionality, low environmental impact, biodegradability, and potential for composting of the final product. However, predominantly, the obtained product should keep the nutritional value of food and protect it against changes in color or shape. Current social transformation has a significant impact on the food packaging sector, on one hand creating a new lifestyle for society all over the world, and on the other, a growing awareness of the negative impact of humans on the environment and increasing responsibility for the planet. The COVID-19 pandemic has highlighted the need to develop a circular economy based on the paradigm of shortening distribution channels, using local raw materials, limiting the consumption of raw materials, energy, water, and above all, minimizing waste production throughout the life cycle of products, all of which are in line with the idea of low-carbon development.

Characterization of propionate CoA-transferase from Ralstonia eutropha H16

2013 ◽  
Vol 98 (8) ◽  
pp. 3579-3589 ◽  
Author(s):  
Elena Volodina ◽  
Marc Schürmann ◽  
Nicole Lindenkamp ◽  
Alexander Steinbüchel
Keyword(s):  
Ralstonia Eutropha ◽  
Coa Transferase ◽  
Ralstonia Eutropha H16

scholarly journals Large scale extraction of poly(3-hydroxybutyrate) from Ralstonia eutropha H16 using sodium hypochlorite

AMB Express ◽  
2012 ◽  
Vol 2 (1) ◽  
pp. 59 ◽  
Author(s):  
Daniel Heinrich ◽  
Mohamed H Madkour ◽  
Mansour A Al-Ghamdi ◽  
Ibraheem I Shabbaj ◽  
Alexander Steinbüchel
Keyword(s):  
Sodium Hypochlorite ◽  
Large Scale ◽  
Ralstonia Eutropha ◽  
Ralstonia Eutropha H16

scholarly journals Cost-Effective Fabrication, Antibacterial Application and Cell Viability Studies of Modified Nonwoven Cotton Fabric

2021 ◽  
Author(s):  
Rahat Nawaz ◽  
Sayed Tayyab Raza Naqvi ◽  
Batool Fatima ◽  
Nazia Zulfiqar ◽  
Muhammad Umer Farooq ◽  
...  
Keyword(s):  
Cotton Fabric ◽  
Biomedical Applications ◽  
Active Sites ◽  
Food Packaging ◽  
Low Cost ◽  
Bacterial Species ◽  
Enterobacter Aerogenes ◽  
Cost Effective ◽  
Bacterial Strains ◽  
Toxicity Assay

Abstract Nonwoven cotton fabric has been fabricated and designed for antibacterial applications using low cost and ecofriendly precursors. The treatment of fabric with alkali leads to formation of active sites. The surfaces were dip coated with silver nanaoparticles and chitosan. The surface was chlorinated in next step to transform amide (N-H) groups in chitosan into N-halamine (N-Cl). The modified and unmodified surfaces of the nonwoven cotton fabric have been characterized by FTIR, SEM, and XRD. The active chlorine loading is measured with iodine/ sodium thiosulphate. The antimicrobial activity and cell toxicity assay were carried out with and without modifications of nonwoven cotton fabric. The antimicrobial efficacies of loaded fabric were evaluated against four bacterial species (Micrococcus lutes, Staphylococcus aurea, Enterobacter aerogenes, and E.coli). It was found that modified fabric exhibited superior efficiency against gram-positive and gram-negative bacterial strains as compared to their bulk counterparts upon exposure without destroying and affecting fabric nature. The overall process is economical for commercial purposes. The modified fabric can be used for antimicrobial, health, and food packaging industries, and in other biomedical applications.

Metabolite profiles of polyhydroxyalkanoate-producing Ralstonia eutropha H16

Metabolomics ◽  
2013 ◽  
Vol 10 (2) ◽  
pp. 190-202 ◽  
Author(s):  
Toshiaki Fukui ◽  
Kenta Chou ◽  
Kazuo Harada ◽  
Izumi Orita ◽  
Yasumune Nakayama ◽  
...  
Keyword(s):  
Ralstonia Eutropha ◽  
Metabolite Profiles ◽  
Ralstonia Eutropha H16

Engineering the CBB cycle and hydrogen utilization pathway of Ralstonia eutropha H16 for improved autotrophic growth and PHB production

2020 ◽  
Author(s):  
Zhongkang Li ◽  
Muzi Hu ◽  
Bin Xiong ◽  
Dongdong Zhao ◽  
Chunzhi Zhang ◽  
...  
Keyword(s):  
Carbon Fixation ◽  
Ralstonia Eutropha ◽  
Biological Research ◽  
Industrial Biotechnology ◽  
Cell Factory ◽  
Autotrophic Growth ◽  
Microbial Cell Factory ◽  
Knock Out ◽  
Ralstonia Eutropha H16 ◽  
Living Organisms

Abstract CO 2 is fixed by all living organisms with an autotrophic metabolism, among which the Calvin-Benson-Bassham ( CBB) cycle is the most important and widespread carbon fixation pathway. Thus, studying and engineering the CBB cycle with the associated energy providing pathways to increase the CO 2 fixation efficiency of cells is an important subject of biological research with significant application potential. In this work, the autotrophic microbe Ralstonia eutropha H16 was selected as a research platform for CBB cycle optimization engineering. By knocking out either CBB operon genes on the operon or mega-plasmid of R. eutropha , we found that both CBB operons were active and contributed almost equally to the carbon fixation process. With similar knock-out experiments, we found while both soluble and membrane-bound hydrogenases (SH and MBH), belonging to the energy providing hydrogenase module, were f unctional d uring autotrophic growth of R. eutropha. And SH played a more significant role. By introducing a heterologous cyanobacterial RuBisCO with the endogenous GroES/EL chaperone system and RbcX, the culture OD 600 of engineered strain increased 89.15% after 72 hours of autotrophic growth, indicating cyanobacterial RuBisCO with a higher activity was functional in R. eutropha and improved upon original CBB pathway. Meanwhile, expression of hydrogenases were optimized by modulating the expression of MBH and SH, which could further increase the R. eutropha H16 culture OD 600 to 93.4% at 72 hours. Moreover, the autotrophic yield of its major industrially relevant product, polyhydroxybutyrate (PHB), was increased by 99.71%. To our best knowledge, this is the first report of successfully engineering the CBB pathway of R. eutropha for improved activity , and is one of only a few cases where the efficiency of CO 2 assimilation pathway was improved. Our work demonstrates that R. eutropha is an extremely useful platform for studying and engineering the CBB for applications in more important organisms, such as agricultural crops, and a potential microbial cell factory to develop industrial biotechnology for sequestrating CO 2 .

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