scholarly journals Cost-Effective Fabrication, Antibacterial Application and Cell Viability Studies of Modified Nonwoven Cotton Fabric

2021 ◽  
Author(s):  
Rahat Nawaz ◽  
Sayed Tayyab Raza Naqvi ◽  
Batool Fatima ◽  
Nazia Zulfiqar ◽  
Muhammad Umer Farooq ◽  
...  
Keyword(s):  
Cotton Fabric ◽  
Biomedical Applications ◽  
Active Sites ◽  
Food Packaging ◽  
Low Cost ◽  
Bacterial Species ◽  
Enterobacter Aerogenes ◽  
Cost Effective ◽  
Bacterial Strains ◽  
Toxicity Assay

Abstract Nonwoven cotton fabric has been fabricated and designed for antibacterial applications using low cost and ecofriendly precursors. The treatment of fabric with alkali leads to formation of active sites. The surfaces were dip coated with silver nanaoparticles and chitosan. The surface was chlorinated in next step to transform amide (N-H) groups in chitosan into N-halamine (N-Cl). The modified and unmodified surfaces of the nonwoven cotton fabric have been characterized by FTIR, SEM, and XRD. The active chlorine loading is measured with iodine/ sodium thiosulphate. The antimicrobial activity and cell toxicity assay were carried out with and without modifications of nonwoven cotton fabric. The antimicrobial efficacies of loaded fabric were evaluated against four bacterial species (Micrococcus lutes, Staphylococcus aurea, Enterobacter aerogenes, and E.coli). It was found that modified fabric exhibited superior efficiency against gram-positive and gram-negative bacterial strains as compared to their bulk counterparts upon exposure without destroying and affecting fabric nature. The overall process is economical for commercial purposes. The modified fabric can be used for antimicrobial, health, and food packaging industries, and in other biomedical applications.

scholarly journals Characterization of Epigallocatechin-Gallate-Grafted Chitosan Nanoparticles and Evaluation of Their Antibacterial and Antioxidant Potential

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1375
Author(s):  
María J. Moreno-Vásquez ◽  
Maribel Plascencia-Jatomea ◽  
Saúl Sánchez-Valdes ◽  
Judith C. Tanori-Córdova ◽  
Francisco J. Castillo-Yañez ◽  
...  
Keyword(s):  
Biomedical Applications ◽  
Food Packaging ◽  
Inhibitory Concentration ◽  
Antioxidant Activities ◽  
Bacterial Species ◽  
Chitosan Nanoparticles ◽  
Epigallocatechin Gallate ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power

Nanoparticles based on chitosan modified with epigallocatechin gallate (EGCG) were synthetized by nanoprecipitation (EGCG-g-chitosan-P). Chitosan was modified by free-radical-induced grafting, which was verified by Fourier transform infrared (FTIR). Furthermore, the morphology, particle size, polydispersity index, and zeta potential of the nanoparticles were investigated. The grafting degree of EGCG, reactive oxygen species (ROS) production, antibacterial and antioxidant activities of EGCG-g-chitosan-P were evaluated and compared with those of pure EGCG and chitosan nanoparticles (Chitosan-P). FTIR results confirmed the modification of the chitosan with EGCG. The EGCG-g-chitosan-P showed spherical shapes and smoother surfaces than those of Chitosan-P. EGCG content of the grafted chitosan nanoparticles was 330 μg/g. Minimal inhibitory concentration (MIC) of EGCG-g-chitosan-P (15.6 μg/mL) was lower than Chitosan-P (31.2 μg/mL) and EGCG (500 μg/mL) against Pseudomonas fluorescens (p < 0.05). Additionally, EGCG-g-chitosan-P and Chitosan-P presented higher Staphylococcus aureus growth inhibition (100%) than EGCG at the lowest concentration tested. The nanoparticles produced an increase of ROS (p < 0.05) in both bacterial species assayed. Furthermore, EGCG-g-chitosan-P exhibited higher antioxidant activity than that of Chitosan-P (p < 0.05) in 2,2′-azino-bis (3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) and ferric-reducing antioxidant power assays. Based on the above results, EGCG-g-chitosan-P shows the potential for food packaging and biomedical applications.

scholarly journals Pan-genome analysis provides much higher strain typing resolution than multi-locus sequence typing

Microbiology ◽  
2010 ◽  
Vol 156 (4) ◽  
pp. 1060-1068 ◽  
Author(s):  
Barry G. Hall ◽  
Garth D. Ehrlich ◽  
Fen Z. Hu
Keyword(s):  
High Resolution ◽  
Low Cost ◽  
Bacterial Species ◽  
Resolving Power ◽  
Multi Locus Sequence Typing ◽  
Strain Typing ◽  
Bacterial Strains ◽  
Clustering Method ◽  
Prognostic Information ◽  
Microarray Analyses

The most widely used DNA-based method for bacterial strain typing, multi-locus sequence typing (MLST), lacks sufficient resolution to distinguish among many bacterial strains within a species. Here, we show that strain typing based on the presence or absence of distributed genes is able to resolve all completely sequenced genomes of six bacterial species. This was accomplished by the development of a clustering method, neighbour grouping, which is completely consistent with the lower-resolution MLST method, but provides far greater resolving power. Because the presence/absence of distributed genes can be determined by low-cost microarray analyses, it offers a practical, high-resolution alternative to MLST that could provide valuable diagnostic and prognostic information for pathogenic bacterial species.

scholarly journals Exploration of Antioxidant, Antibacterial Activities of Green Synthesized Hesperidin Loaded PLGA Nanoparticles

2021 ◽  
Vol 11 (6) ◽  
pp. 14520-14528
Keyword(s):  
Enterobacter Aerogenes ◽  
Cost Effective ◽  
Antibacterial Activities ◽  
Plga Nanoparticles ◽  
Bacterial Strains ◽  
Antioxidants Activity ◽  
Good Ability ◽  
Potential Benefits ◽  
Greener Synthesis ◽  
Dose Dependent

To elaborate on the potential benefits of hesperidin loaded PLGA nanoparticles (HES-PLGA NPs), present investigation reports about the antioxidant, antimicrobial, and cytotoxic potential of hesperidin loaded PLGA nanoparticles (HES-PLGA-NPs). The antioxidant potential of Hesperidin and HES-PLGA-NPs was demonstrated by ATBS, DPPH, Hydroxy, Hydrogen peroxide, and superoxide radical showing the dose-dependent antioxidants activity. Both the test gained better potential with HES loaded PLGA nanoparticles than free HES. HES-PLGA-NPs also proved its antibacterial activity against tested bacterial strains, including Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, and Pseudomonas aeruginosa. HES-PLGA-NPs had a good ability to minimize biofilms' formation and a good to weak ability to degrade pre-formed biofilms. Thus, the bionanotherapy, which has been attempted using HES-PLGA-NPs would provide a promising scope as a potent bio compound that can be replaced for medical ailments in a cost-effective, greener synthesis.

scholarly journals Development of a Benzalkonium Chloride Based Antibacterial Paper for Health and Food Applications

2021 ◽  
Vol 5 (1) ◽  
pp. 1
Author(s):  
Swarit Ahmed Shadman ◽  
Ishmamul Hoque Sadab ◽  
Mohammed Sakib Noor ◽  
Mohidus Samad Khan
Keyword(s):  
Benzalkonium Chloride ◽  
Food Packaging ◽  
Pathogenic Bacteria ◽  
Low Cost ◽  
Ammonium Compound ◽  
Bacterial Strains ◽  
Allowable Limit ◽  
The Public ◽  
Food Applications ◽  
Spread Of Infection

Pathogenic bacteria and other microorganisms pose a potent threat to humans by causing various infectious diseases. To control the spread of infection, different antibacterial products have been developed. However, most of them are known to be associated with health hazards, environmental pollution, complex fabrication, and/or higher cost. To address these issues, in this study, a low cost, biodegradable and human skin compatible antibacterial paper has been developed. A quaternary ammonium compound, benzalkonium chloride (BKC) has been used for paper surface treatment. The concentration of aqueous solution of BKC coated on paper was varied from 0.1 wt% to 0.2 wt%. No external binder was required for coating BKC onto paper. The efficacy of the coated paper was investigated against Staphylococcus aureus ATCC 6538 and Escherichia coli ATCC 8739 bacterial strains. This antibacterial paper is highly effective against both strains with the concentrations of BKC being within the allowable limit for cytotoxic effects. The optimum concentration of BKC coated on paper can be considered as 0.15 wt%, as nearly 100% inhibition was achieved with it against both strains. The developed antibacterial paper is suitable for being used in the industry for disinfection and food packaging purposes, and also by the public for hand sanitization.

scholarly journals Effect of Rhamnolipids on Pathogenicity Characteristics of Microorganisms in Organic Compost

2021 ◽  
Vol 9 (1) ◽  
pp. 39-50
Author(s):  
Ciamak Ghazaei ◽  
Keyword(s):  
Pathogenic Bacteria ◽  
Bacillus Stearothermophilus ◽  
Bacterial Species ◽  
Enterobacter Aerogenes ◽  
Antimicrobial Effect ◽  
Inhibitory Effect ◽  
Biological Research ◽  
Bacterial Strains ◽  
Antiviral Activities ◽  
Organic Compost

Background: Rhamnolipids are hydrophilic glycolipids, often classified as biosurfactants. They are produced by different bacterial species. Rhamnolipids are extensively studied in biological research because of their interesting features like antimicrobial, antifungal, and antiviral activities. Materials and Methods: To study the antimicrobial effect of rhamnolipid, we conducted a crosssectional study on the eight different pathogenic bacterial strains from November 2019 to June 2020. These bacterial strains were isolated from the organic compost. Both disk diffusion and broth microdilution methods were used to evaluate the inhibitory effects of rhamnolipids on these pathogenic bacteria. Also, protease and amylase enzyme activities were evaluated in these eight bacterial isolates. Results: For Bacillus stearothermophilus, within the area of 31.5 mm, no growth was observed, hence proving the inhibitory effect of rhamnolipid. After calculating the minimum inhibitory (MIC) and minimum lethal concentrations (MLC) for each bacterial strain, it has been found that the studied bacteria were more susceptible to rhamnolipids than most of the antibiotics. Strains were also quantified for their enzymatic activity of proteases and amylases. The bacterial strains of Bacillus stearothermophilus, Brevibacillus brevis, Bacillus licheniformis, and Bordetella petrii showed maximum protease activity. Whereas Escherichia coli, Enterococcus faecalis, Enterobacter aerogenes, and Pseudomonas aeruginosa showed high amylase activity. Conclusion: Rhamnolipids can be used as a potential antimicrobial agent for treatment of infections.

scholarly journals Novel biotechnological approaches for monitoring and immunization against resistant to antibiotics Escherichia coli and other pathogenic bacteria

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
José E. Belizário ◽  
Marcelo P. Sircili
Keyword(s):  
Escherichia Coli ◽  
Bacterial Infections ◽  
Pathogenic Bacteria ◽  
Low Cost ◽  
Methyl Esters ◽  
Cost Effective ◽  
Antibiotic Use ◽  
Immunological Methods ◽  
Bacterial Strains ◽  
Surveillance Programs

Abstract The application of next-generation molecular, biochemical and immunological methods for developing new vaccines, antimicrobial compounds, probiotics and prebiotics for zoonotic infection control has been fundamental to the understanding and preservation of the symbiotic relationship between animals and humans. With increasing rates of antibiotic use, resistant bacterial infections have become more difficult to diagnose, treat, and eradicate, thereby elevating the importance of surveillance and prevention programs. Effective surveillance relies on the availability of rapid, cost-effective methods to monitor pathogenic bacterial isolates. In this opinion article, we summarize the results of some research program initiatives for the improvement of live vaccines against avian enterotoxigenic Escherichia coli using virulence factor gene deletion and engineered vaccine vectors based on probiotics. We also describe methods for the detection of pathogenic bacterial strains in eco-environmental headspace and aerosols, as well as samples of animal and human breath, based on the composition of volatile organic compounds and fatty acid methyl esters. We explain how the introduction of these low-cost biotechnologies and protocols will provide the opportunity to enhance co-operation between networks of resistance surveillance programs and integrated routine workflows of veterinary and clinical public health microbiology laboratories.

scholarly journals Isolation of Two Bacterial Species from Argan Soil in Morocco Associated with Polyhydroxybutyrate (PHB) Accumulation: Current Potential and Future Prospects for the Bio-Based Polymer Production

Polymers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 1870
Author(s):  
Amina Aragosa ◽  
Valeria Specchia ◽  
Mariaenrica Frigione
Keyword(s):  
Low Cost ◽  
Bacterial Species ◽  
Industrial Applications ◽  
Industrial Wastes ◽  
Bacterial Strains ◽  
Natural Polymers ◽  
Sudan Black B ◽  
Sugar Fermentation ◽  
Wide Range ◽  
Biochemical Analyses

The environmental issues caused by the impacts of synthetic plastics use and derived wastes are arising the attention to bio-based plastics, natural polymers produced from renewable resources, including agricultural, industrial, and domestic wastes. Bio-based plastics represent a potential alternative to petroleum-based materials, due to the insufficient availability of fossil resources in the future and the affordable low cost of renewable ones that might be consumed for the biopolymer synthesis. Among the polyhydroxyalkanoates (PHA), the polyhydroxybutyrate (PHB) biopolymer has been synthesized and characterized with great interest due to its wide range of industrial applications. Currently, a wide number of bacterial species from soil, activated sludge, wastewater, industrial wastes, and compost have been identified as PHB producers. This work has the purpose of isolating and characterizing PHB-producing bacteria from the agricultural soil samples of Argania spinosa in the south region of Morocco where the plant species is endemic and preserved. During this research, four heat-resistant bacterial strains have been isolated. Among them, two species have been identified as endospore forming bacteria following the Schaffer-Fulton staining method with Malachite green and the Methylene blue method. Black intracellular granules have been appreciated in microscopy at 100× for both strains after staining with Sudan black B. The morphological and biochemical analyses of the isolates, including sugar fermentation and antibiotic susceptibility tests, preliminarily identified the strains 1B and 2D1 belonging to the genus Serratia and Proteus, respectively.

scholarly journals Protamine-like proteins have bactericidal activity. The first evidence in Mytilus galloprovincialis

2018 ◽  
Author(s):  
Rosaria Notariale ◽  
Adriana Basile ◽  
Elena Montana ◽  
Nunzia Colonna Romano ◽  
Maria Grazia Cacciapuoti ◽  
...  
Keyword(s):  
Bactericidal Activity ◽  
Mytilus Galloprovincialis ◽  
Bacterial Species ◽  
Enterobacter Aerogenes ◽  
Sperm Chromatin ◽  
Bacterial Strains ◽  
Gram Positive ◽  
Gram Negative ◽  
Intermediate Group

The major acid-soluble protein components of the mussel Mytilus galloprovincialis sperm chromatin consist of the protamine-like proteins PL-II, PL-III and PL-IV, an intermediate group of sperm nuclear basic proteins between histones and protamines. The aim of this study was to investigate the bactericidal activity of these proteins since, to date, there are reports on bactericidal activity of protamines and histones, but not on protamine-like proteins. We tested the bactericidal activity of these proteins against Gram-positive bacteria: Enterococcus faecalis and two different strains of Staphylococcus aureus, as well as Gram-negative bacteria: Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Salmonella typhmurium, Enterobacter aerogenes, Enterobacter cloacae, and Escherichia coli. Clinical isolates of the same bacterial species were also used to compare their sensitivity to these proteins. The results show that Mytilus galloprovincialis protamine-like proteins exhibited bactericidal activity against all bacterial strains tested with different minimum bactericidal concentration values, ranging from 15.7 to 250 µg/mL. Furthermore, these proteins were active against some bacterial strains tested that are resistant to conventional antibiotics. These proteins showed very low toxicity as judged by red blood cell lysis and viability MTT assays and seem to act both at the membrane level and within the bacterial cell. We also tested the bactericidal activity of the product obtained from an in vitro model of gastrointestinal digestion of protamine-like proteins on a Gram-positive and a Gram-negative strain, and obtained the same results with respect to undigested protamine-like proteins on the Gram-positive bacterium. These results provide the first evidence of bactericidal activity of protamine-like-proteins.

Synthesis of Value Added Nanoparticles from Biomass Resources

2021 ◽  
Vol 25 (11) ◽  
pp. 175-183
Author(s):  
Gobinda Prasad Chutia
Keyword(s):  
Biomedical Applications ◽  
Food Packaging ◽  
Low Cost ◽  
Value Added ◽  
Green Energy ◽  
Practical Applications ◽  
Biomass Resources ◽  
Biomass Materials ◽  
Biological Methods ◽  
Potential Use

The utilization of sustainable biomass materials provides a versatile route for the development of new alternatives to replace traditional petro-materials for a variety of purposes such as green energy, paint, food packaging and biomedical applications. This study reviews the potential use of various sustainable biomass materials for the production of low-cost, highvalue- added materials for practical applications including bio-printing, drug delivery/controlled release, tissue engineering, energy storage and biosensing. This study highlights the fabrication of novel nanomaterials from various biowastes including crop residue, food waste and industrial waste (e.g. spent battery waste and polythene waste) through physical, chemical, or biological methods.

scholarly journals Rapid prototyping of a novel and flexible paper based oxygen sensing patch via additive inkjet printing process

RSC Advances ◽  
2019 ◽  
Vol 9 (39) ◽  
pp. 22695-22704 ◽  
Author(s):  
Dinesh Maddipatla ◽  
Binu B. Narakathu ◽  
Manuel Ochoa ◽  
Rahim Rahimi ◽  
Jiawei Zhou ◽  
...  
Keyword(s):  
Rapid Prototyping ◽  
Inkjet Printing ◽  
Manufacturing Process ◽  
Biomedical Applications ◽  
Food Packaging ◽  
Oxygen Sensing ◽  
Low Cost ◽  
Printing Process ◽  
Cost Efficient ◽  
First Time

A paper-based low cost and rapid prototypable flexible oxygen sensing patch was developed for the first time using a cost-efficient additive inkjet print manufacturing process for wearable, food packaging, pharmaceutical and biomedical applications.

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