scholarly journals Composition and Antioxidant Activity, Supercritical Carbon Dioxide Extraction Extracts, and Residue after Extraction of Biologically Active Compounds from Freeze-Dried Tomato Matrix

Processes ◽  
2021 ◽  
Vol 9 (3) ◽  
pp. 467
Author(s):  
Dalia Urbonavičienė ◽  
Česlovas Bobinas ◽  
Ramunė Bobinaitė ◽  
Lina Raudonė ◽  
Sonata Trumbeckaitė ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Antioxidant Activity ◽  
Supercritical Carbon Dioxide ◽  
Biologically Active ◽  
Scavenging Activity ◽  
Supercritical Carbon Dioxide Extraction ◽  
C6 Cells ◽  
Active Compounds ◽  
Freeze Dried ◽  
Carbon Dioxide Extraction

Supercritical carbon dioxide extraction (SCE-CO2) is an attractive, green technology that is used for the recovery of biologically active compounds from plant material. The antioxidant potential of lipophilic fractions (extract obtained with SCE-CO2) and hydrophilic fractions (extracts obtained from the residue after extraction) obtained from a matrix of freeze-dried tomatoes (cvs. “Admiro” F1, “Jurgiai”, “Vilina”, “Pirmutis”, and “Skariai”) was assessed via different antioxidant activity methods. The total amount of polyphenols, carotenoids, and carotenoid isomers before and after SCE-CO2 extraction was also determined. To investigate the effect of the SCE-CO2 extract on the viability of cancer cells, rat glioblastoma C6 cells were chosen. The SCE-CO2 yielded an average of 800 mg of lipophilic fraction per 100 g of freeze-dried tomatoes. The ABTS•+ scavenging activity of the extract was 251 ± 3.4 µmol TE/g. After SCE-CO2 extraction, the DPPH•-RSA of the freeze-dried tomato matrix was 7 to 12% higher. There was a strong positive correlation (R = 0.84) between the total polyphenolics content and the DPPH•-RSA of the tomato samples. The SCE-CO2 increased the radical scavenging activity of the extraction residue, indicating that a considerable fraction of the hydrophilic compounds with particular antioxidant capacity remain unextracted from the tomato matrix. Our results reveal the cytotoxic effect of lycopene extract rich in cis-isomers (62% cis-isomers of the total lycopene content) on rat glioblastoma C6 cells. The viability of the glioblastoma C6 cells significantly decreased (−42%) at a total lycopene concentration of 2.4 µM after 24 h of incubation.

Modified Supercritical Carbon Dioxide Extraction of Biologically Active Compounds from Feijoa Sellowiana Leaves

2019 ◽  
Vol 15 (7) ◽  
Author(s):  
Mandana Bimakr ◽  
Seyyed Mohammad Ghoreishi ◽  
Ali Ganjloo ◽  
Mitra Mousavi
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Total Yield ◽  
Ultrasonic Waves ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Supercritical Carbon Dioxide Extraction ◽  
Active Compounds ◽  
Carbon Dioxide Extraction ◽  
Supercritical Carbon

AbstractFor the first time, the modified supercritical carbon dioxide extraction (MSCE) of biologically active compounds from Feijoa sellowiana leaves was investigated. Total yield (TY) and bioactivity of biocompounds in terms of DPPH• (%DPPHsc) and HO• (%HOsc) radicals scavenging were maximized via multivariate optimization. The optimized conditions were the pressure of 272.34 bar, supercritical carbon dioxide flow rate of 1.58 mL min−1, temperature of 52.48 °C, and dynamic extraction time of 110.62 min. The predicted values of the TY, %DPPHsc, and %HOsc were 81.12 mg g−1, 85.16 %, and 75.66 %, respectively. The chromatographic analysis demonstrated gallic acid as the main biophenolic (136.20 ± 0.16 mg g−1). The scanning electron microscopy images showed dramatic disruption of cells treated with MSCE. A comprehensive comparison was made between current findings and other techniques (conventional and ultrasonic waves assisted extraction). More TY was achieved using conventional method but the bioactivity of MSCE products was considerably better.

scholarly journals Cytotoxic Deoxypodophyllotoxin Can Be Extracted in High Purity from Anthriscus sylvestris Roots by Supercritical Carbon Dioxide

Planta Medica ◽  
2017 ◽  
Vol 84 (08) ◽  
pp. 544-550 ◽  
Author(s):  
Christel Seegers ◽  
Pieter Tepper ◽  
Rita Setroikromo ◽  
Wim Quax
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Biologically Active ◽  
Supercritical Carbon Dioxide Extraction ◽  
High Demand ◽  
Polar Components ◽  
Anthriscus Sylvestris ◽  
Carbon Dioxide Extraction ◽  
M Phase ◽  
Supercritical Carbon

AbstractDeoxypodophyllotoxin is present in the roots of Anthriscus sylvestris. This compound is cytotoxic on its own, but it can also be converted into podophyllotoxin, which is in high demand as a precursor for the important anticancer drugs etoposide and teniposide. In this study, deoxypodophyllotoxin is extracted from A. sylvestris roots by supercritical carbon dioxide extraction. The process is simple and scalable. The supercritical carbon dioxide method extracts 75 – 80% of the total deoxypodophyllotoxin content, which is comparable to a single extraction by traditional Soxhlet. However, less polar components are extracted. The activity of the supercritical carbon dioxide extract containing deoxypodophyllotoxin was assessed by demonstrating that the extract arrests A549 and HeLa cells in the G2/M phase of the cell cycle. We conclude that biologically active deoxypodophyllotoxin can be extracted from A. sylvestris by supercritical carbon dioxide extraction. The method is solvent free and more sustainable compared to traditional methods.

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