scholarly journals Poultry Slaughterhouse Wastewater Remediation Using a Bio-Delipidation Pre-Treatment Unit Coupled with an Expanded Granular Sludge Bed Reactor

Processes ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1938
Author(s):  
Cebisa Thabo Mdladla ◽  
Phumeza Akhona Dyosile ◽  
Mahomet Njoya ◽  
Moses Basitere ◽  
Seteno Karabo Obed Ntwampe ◽  
...  

The treatment of poultry slaughterhouse wastewater (PSW) with an Expanded Granular Sludge-Bed Bioreactor (EGSB) is hindered by the accumulation and washout of sludge, and difficulties associated with the operation of the three-phase separator and the determination of the optimum up-flow velocity for sludge-bed fluidization. This results in a poor reactor functionality, and thus a poor performance due to fats, oil and grease (FOG) in the PSW being treated. Hydrolyzing the FOG content with a bio-delipidation, enzyme-based agent in a pre-treatment unit would significantly improve the effectiveness of the EGSB. In this study, PSW was pre-treated for 48 h with a biological mixture containing bioflocculants and bio-delipidation constituents. The pre-treated PSW was further treated in an EGSB. The PSW FOG, total chemical oxygen demand (tCOD) and total suspended solids (TSS) content were determined to assess the effectiveness of the pre-treatment process as well as to observe the remedial action of the combined pre-treatment-EGSB system. An increased treatment efficacy was noted for the combined PSW treatment system, whereby the COD, FOG and TSS removal averaged 76%, 88% and 87%, respectively. The process developed is intended for micro, small and medium poultry slaughterhouses.

Membranes ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 582
Author(s):  
Phumeza Akhona Dyosile ◽  
Cebisa Mdladla ◽  
Mahomet Njoya ◽  
Moses Basitere ◽  
Seteno Karabo Obed Ntwampe ◽  
...  

This paper assesses the performance of an integrated multistage laboratory-scale plant, for the treatment of poultry slaughterhouse wastewater (PSW). The system was comprised of an eco-flush dosed bio-physico pre-treatment unit for fats, oil, and grease (FOG) hydrolysis prior to the PSW being fed to a down-flow expanded granular bed reactor (DEGBR), coupled to a membrane bioreactor (DEGBR-MBR). The system’s configuration strategy was developed to achieve optimal PSW treatment by introducing the enzymatic pre-treatment unit for the lipid-rich influent (PSW) in order to treat FOG including odour causing constituents such as H2S known to sour anaerobic digestion (AD) such that the PSW pollutant load is alleviated prior to AD treatment. This was conducted to aid the reduction in clogging and sludge washout in the DEGBR-MBR systems and to achieve the optimum reactor and membrane system performance. A performance for the treatment of PSW after lipid reduction was conducted through a qualitative analysis by assessing the pre- and post-pre-treatment units’ chemical oxygen demand (COD), total suspended solids (TSS), and FOG concentrations across all other units and, in particular, the membrane units. Furthermore, a similar set-up and operating conditions in a comparative study was also performed. The pre-treatment unit’s biodelipidation abilities were characterised by a mean FOG removal of 80% and the TSS and COD removal reached 38 and 56%, respectively. The final acquired removal results on the DEGBR, at an OLR of ~18–45 g COD/L.d, was 87, 93, and 90% for COD, TSS, and FOG, respectively. The total removal efficiency across the pre-treatment-DEGBR-MBR units was 99% for COD, TSS, and FOG. Even at a high OLR, the pre-treatment-DEGBR-MBR train seemed a robust treatment strategy and achieved the effluent quality set requirements for effluent discharge in most countries.


2016 ◽  
Vol 11 (1) ◽  
pp. 86-92 ◽  
Author(s):  
M. Basitere ◽  
Y. Williams ◽  
M. S. Sheldon ◽  
S. K. O. Ntwampe ◽  
D. De Jager ◽  
...  

Generally, slaughterhouses have the largest consumption of fresh water and thus generate large quantities of high strength wastewater, which can be treated successfully using low cost biological treatment processes. In this study, the feasibility of using an expanded granular sludge bed (EGSB) anaerobic reactor coupled with anoxic and aerobic bioreactors for the treatment of poultry slaughterhouse wastewater was investigated. The poultry slaughterhouse was characterized by high chemical oxygen demand (COD), 2 to 6 g/L, with average biological oxygen demand of 2.4 g/L and average fats, oil and grease (FOG) being 0.55 g/L. A continuous EGSB anaerobic reactor was operated for 26 days at different hydraulic retention times (HRT), i.e. 7, 4, 3 days, and organic loading rates (OLR) of 0.5, 0.7 and 1.0 g COD/L.day, respectively, to assess the bioremediation of the poultry slaughterhouse wastewater. The average COD removal from the EGSB was 40%, 57% and 55% at the different OLR and HRT assessed. At high OLR of 1.0 g COD/L.day, the overall COD removal from the system (EGSB-anoxic/aerobic) averaged 65%. The system experienced periodical sludge washout during high FOG and suspended solids loading. It was concluded that the EGSB system requires a dissolved air flotation system, for FOG/suspended solid reduction, as the performance of the overall system was observed to deteriorate over time due to the presence of a high quantity of FOG including suspended solids.


2019 ◽  
Vol 14 (3) ◽  
pp. 549-559 ◽  
Author(s):  
M. Njoya ◽  
M. Basitere ◽  
S. K. O. Ntwampe

Abstract This study evaluated the performance of a novel high rate anaerobic bioreactor system for the treatment of poultry slaughterhouse wastewater (PSW). The new system consisted of a granule-based technology operated in a down-flow configuration, with the assistance of medium-sized pumice stones used as packing materials for the retention of the anaerobic granules, to avoid challenges associated with the use of the three-phase separator of up-flow systems and the washout of the anaerobic biomass. Furthermore, a recycling stream was applied to the system to improve the mixing inside the Down-flow Expanded Granular Bed Reactor (DEGBR), i.e. the influent distribution to the granular biomass, and the implementation of intermittent fluidization when required to alleviate the effects of pressure drop in such systems. The DEGBR was operated under mesophilic conditions (30–35 °C) and achieved total chemical oxygen demand (tCOD), five-day biological oxygen demand and total suspended solids average removal percentages >95%, and a fats, oils and grease average removal percentage of 93.67% ± 4.51, for an organic loading rate varying between 1.1 to 38.9 gCOD/L.day.


Membranes ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 345
Author(s):  
Honeil Basile Meyo ◽  
Mahomet Njoya ◽  
Moses Basitere ◽  
Seteno Karabo Obed Ntwampe ◽  
Ephraim Kaskote

This study presents the biological treatment of poultry slaughterhouse wastewater (PSW) using a combination of a biological pretreatment stage, an expanded granular sludge bed reactor (EGSB), and a membrane bioreactor (MBR) to treat PSW. This PSW treatment was geared toward reducing the concentration of contaminants present in the PSW to meet the City of Cape Town (CoCT) discharge standards and evaluate an alternative means of treating medium- to high-strength wastewater at low cost. The EGSB used in this study was operated under mesophilic conditions and at an organic loading rate (OLR) of 69 to 456 mg COD/L·h. The pretreatment stage of this laboratory-scale (lab-scale) plant played an important role in the pretreatment of the PSW, with removal percentages varying between 20% and 50% for total suspended solids (TSS), 20% and 70% for chemical oxygen demand (COD), and 50% and 83% for fats, oil, and grease (FOG). The EGSB further reduced the concentration of these contaminants to between 25% and 90% for TSS, 20% and 80% for COD, and 20% and >95% for FOG. The last stage of this process, i.e., the membrane bioreactor (MBR), contributed to a further decrease in the concentration of these contaminants with a peak removal performance of >95% for TSS and COD and 80% for the FOG. Overall, the system (pretreatment–EGSB–MBR) exceeded 97% for TSS and COD removal and 97.5% for FOG removal. These results culminated in a product (treated wastewater) meeting the discharge standards.


2021 ◽  
Vol 11 (14) ◽  
pp. 6536
Author(s):  
Derrick Njabuliso Dlamini ◽  
Moses Basitere ◽  
Mahomet Njoya ◽  
Seteno Karabo Obed Ntwampe ◽  
Ephraim Kaskote

Poultry slaughterhouse wastewater contains high concentrations of chemical oxygen demand (COD), total suspended solids (TSSs), fats, oil and grease (FOG), proteins and carbohydrates. It is important that the wastewater is treated to acceptable environmental discharge standards. In this study, the poultry slaughterhouse wastewater (PSW) was treated using two-stage processes consisting of a biological pre-treatment using a biodegrading agent (Eco-flushTM) coupled with a down-flow expanded granular bed reactor (DEGBR). The results showed that the biological pre-treatment was observed to be highly effective for removal of FOG, COD and TSS with a removal efficiency of 80 ± 6.3%, 38 ± 8.4% and 56 ± 7.2%, respectively. The DEGBR showed a stable performance in terms FOG, COD and, TSS removal, with average removal efficiencies of 89 ± 2.8%, 87 ± 9.5%, and 94 ± 3.7%, respectively. The overall removal rate performance of the integrated system of pre-treatment and DEGBR in terms FOG, COD and TSS, was 97 ± 0.8%, 92 ± 6.3% and 97 ± 1.2%. Furthermore, the average volatile fatty acid/alkalinity (VFA/Alkalinity) ratio of 0.2 was reported, which indicated that the DEGBR was stable throughout the operation.


2017 ◽  
Vol 76 (1) ◽  
pp. 106-114 ◽  
Author(s):  
M. Basitere ◽  
Z. Rinquest ◽  
M. Njoya ◽  
M. S. Sheldon ◽  
S. K. O. Ntwampe

The South African poultry industry has grown exponentially in recent years due to an increased demand for their products. As a result, poultry plants consume large volumes of high quality water to ensure that hygienically safe poultry products are produced. Furthermore, poultry industries generate high strength wastewater, which can be treated successfully at low cost using anaerobic digesters. In this study, the performance of a bench-scale mesophilic static granular bed reactor (SGBR) containing fully anaerobic granules coupled with an ultrafiltration (UF) membrane system, as a post-treatment system, was investigated. The poultry slaughterhouse wastewater was characterized by a chemical oxygen demand (COD) range between 1,223 and 9,695mg/L, average biological oxygen demand of 2,375mg/L and average fats, oil and grease (FOG) of 554mg/L. The SGBR anaerobic reactor was operated for 9 weeks at different hydraulic retention times (HRTs), i.e. 55 and 40 h, with an average organic loading rate (OLR) of 1.01 and 3.14g COD/L.day. The SGBR results showed an average COD, total suspended solids (TSS) and FOG removal of 93%, 95% and 90% respectively, for both OLR. The UF post-treatment results showed an average of COD, TSS and FOG removal of 64%, 88% and 48%, respectively. The overall COD, TSS and FOG removal of the system (SGBR and UF membrane) was 98%, 99.8%, and 92.4%, respectively. The results of the combined SGBR reactor coupled with the UF membrane showed a potential to ensure environmentally friendly treatment of poultry slaughterhouse wastewater.


2009 ◽  
Vol 60 (1) ◽  
pp. 243-249 ◽  
Author(s):  
A. B. G. Valladão ◽  
P. E. Sartore ◽  
D. M. G. Freire ◽  
M. C. Cammarota

The effect of different hydrolysis times (4, 8 and 24 h) of a lipase-rich enzymatic preparation (0.1, 0.5 and 1.0% w/v) produced by fungus Penicillium sp. in solid-state fermentation was evaluated on the anaerobic biodegradability of a poultry slaughterhouse wastewater with 800 mg oil and grease [O&G]/L in three sequential batches. The enzymatic pre-treatment of O&G in the experiments with no acclimated sludge had no discernible effect because regardless of the conditions adopted COD removal efficiencies of 95.3% to 98.7% were obtained. However, when the sludge was reused (once or twice) the COD removal efficiencies in the control experiments (69.8% and 53.4%) were considerably lower than in the experiments with hydrolyzed effluent (of 93.8% to 98.4%). Higher values of specific methane production were obtained with 0.1% SEP and 4 h of hydrolysis. After acclimation of the sludge, 19.9% fat was still adhered to the sludge in the control experiment, while the sludge in the experiment with enzymatically pre-treated effluent contained only 8.6% fat, confirming the accumulation of fat when the enzyme pool was not used.


Author(s):  
Siyasanga Mbulawa ◽  
Seteno Karabo Obed Ntwampe ◽  
Moses Basitere ◽  
Yolanda Mpentshu ◽  
Cynthia Dlangamandla ◽  
...  

Delipidation is a method of defatting that is generally associated with the removal of residual lipids or lipid groups from matrices in which they are present in minute quantities. The bio-delipidation of protein-rich poultry slaughterhouse wastewater (PSW) pre-treated with a dissolved air flotation (DAF) system was developed using microbial lipases from bacterial strains isolated from the PSW. The efficacy of the bio-delipidation system was quantitatively characterised by comparing the quality parameters i.e., fats, oil and grease (FOGs), turbidity, total suspended solutes (TSS), total chemical oxygen demand (tCOD) and protein concentration of the DAF pre-treated PSW and bio-lipidized samples. As hypothesised, the bio-delipidation system was able to effectively reduce the levels of these quality parameters when crude lipases of Bacillus cereus AB1 (BF3) and Bacillus cereus CC-1 (B30) strains were used. Strain-dependent quality characteristics were also observed in bio-delipidized samples. The study successfully managed to complement physical reduction techniques (DAF) with biological strategies (bio-delipidation) for improved PSW quality, with potential industrial applications.


1995 ◽  
Vol 32 (11) ◽  
pp. 55-63 ◽  
Author(s):  
S. K. I. Sayed ◽  
M. A. A. Fergala

A multi-target study was conducted to assess the feasibility of the UASB-reactor system for the anaerobic treatment of raw domestic sewage (low-strength complex wastewater; CODtot=200-700 mg/l and CODss=45-55% of CODtot) combined with sludge stabilization process under a moderate temperature of 18-20 °C. The study has produced a design of a modified Three-Phase Separator (TPS) to control the sludge retention inside the UASB reactors as well as the determination of design parameters of the treatment process viz. the hydraulic retention time (HRT), the potential period of loading the UASB reactors and the most convenient digestion time required for advanced sludge stabilization. The study was performed in a Two-Stage Flocculent-Granular-Sludge UASB-reactor system. The first stage consisted of two identical UASB reactors seeded with flocculent sludge and operated alternately (i.e. one reactor was fed while the other was unfed for the stabilization of the sludge). The second stage consisted of one UASB reactor seeded with granular sludge and was operated continuously. The feed period and the corresponding feedless period of the first stage were two days. The results study have shown that the removal efficiency of the COD increases considerably with the decrease of the HRT, as a treatment efficiency of 75% was achieved at HRT=10 h (8 h for Stage I and 2 h for Stage II), while the treatment efficiency was 84% at HRT=6 h (4 h for Stage I and 2 h for Stage II). In contrast to the above the extent of sludge stabilization (i.e. conversion into methane) for the first stage of the system was directly proportional to the HRT as 41% of the removed CODtot was converted into methane at HRT=8 h, the conversion ratio decreased to 28% at HRT=4 h. For the granular sludge (the second stage of the system), the conversion ratio of the removed CODtot into methane was almost stable in the range of 17-20%. The percentages CODpaper-filtered of the CODtot were 85-90% and 92-97% for the effluents of the first and second stages respectively with the conclusion that the modified Three-Phase Separator (TPS) has a satisfactory operational potential.


Author(s):  
Philadelphia Vutivi Ngobeni ◽  
Moses Basitere ◽  
Andile Thole

Abstract Poultry slaughterhouses are generally large consumers of fresh water, which is exhausted as wastewater characterized by a high concentration of biological oxygen demand (BOD), chemical oxygen demand COD, and fats, oil, and grease (FOG). Cost-effective methods are required for the treatment of poultry slaughterhouse wastewater, with the aim of attaining a high quality effluent that can be reused in industrial processes to promote sustainability. As compared to conventional treatment methods, electrocoagulation is an efficient and low-cost system. Electrocoagulation is environmentally friendly, treating wastewater without the need of chemicals, thus limiting secondary pollution. The metal anodes initiate electrochemical reactions for coagulation and flocculation. Its distinct advantages include compact installation, and simple operation. This paper offers a comprehensive review of recent literature that has been dedicated to utilizing electrocoagulation for poultry slaughterhouse wastewater treatment. This paper also examines aspects such as theory, potential applications, current applications, as well as economical assessment of the technique.


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