Lactic Acid Bacteria Mediated Apoptosis Induction: Natural Way of Colon Cancer Cells’ Inhibition

One of the compounds found efficacious as an anti-proliferative on colon cancer is alpha-mangosteen, a xanthon compound that is abundant in pericarp of mangosteen. This study focused to evaluate anticancer activity of the ethanolic extract of pericarp of mangosteen (Garcinia mangostana Linn.) on WiDr colon cancer cells and to observe the affinity of alpha-mangosteen in inhibiting IKK and VEGF. Cytotoxic effect was tested by MTT assay and apoptosis induction was evaluated by double staining method on WiDr colon cancer cells, while interaction between alpha-mangosteen to the receptors was measured by molecular docking. The ethanolic extract was proven to have cytotoxic activity against WiDr colon cancer cells with IC50 of 25 µg/mL. In addition, the observation of apoptosis induction by double-staining method showed that apoptosis associated the cytotoxic effect of ethanolic extract of pericarp of mangosteen (EPM) on WiDr colon cancer cells. Molecular docking showed that active compounds in pericarp of mangosteen could compete with the native ligand of VEGF because the docking score of alpha-mangosteen was almost equal with native ligand. From these results we could be concluded that the cytotoxic effect of EPM to WiDr cells was mediated by cell apoptosis. This extract was potential to be developed as chemopreventive agent.Keywords : Garcinia mangostana Linn., cytotoxic effect, apoptosis, WiDr cell, molecular docking

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Bcl-2 overexpression attenuates apoptosis induction by sulindac sulfide in SW480 human colon cancer cells

Journal of Clinical Oncology ◽

10.1200/jco.2004.22.90140.3195 ◽

2004 ◽

Vol 22 (14_suppl) ◽

pp. 3195-3195

Author(s):

R. Penington ◽

F. Sinicrope

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Human Colon ◽

Apoptosis Induction ◽

Colon Cancer Cells ◽

Human Colon Cancer ◽

Sulindac Sulfide ◽

Human Colon Cancer Cells

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Apoptotic Cell Death and Inhibition of Wnt/β-Catenin Signaling Pathway in Human Colon Cancer Cells by an Active Fraction (HS7) fromTaiwanofungus camphoratus

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/750230 ◽

2011 ◽

Vol 2011 ◽

pp. 1-13 ◽

Cited By ~ 14

Author(s):

Chi-Tai Yeh ◽

Chih-Jung Yao ◽

Jiann-Long Yan ◽

Shuang-En Chuang ◽

Liang-Ming Lee ◽

...

Keyword(s):

Colon Cancer ◽

Cancer Cells ◽

Human Colon ◽

Dependent Manner ◽

Apoptosis Induction ◽

Colon Cancer Cells ◽

Active Fraction ◽

Human Colon Cancer ◽

Taiwanofungus Camphoratus ◽

Human Colon Cancer Cells

Aberrant activation of Wnt/β-catenin signaling plays an important role in the development of colon cancer. HS7 is an active fraction extracted fromTaiwanofungus camphoratus, which had been widely used as complementary medicine for Taiwan cancer patients in the past decades. In this study, we demonstrated the effects of HS7 on the growth inhibition, apoptosis induction, and Wnt/β-catenin signaling suppression in human colon cancer cells. HS7 significantly inhibited proliferation of HT29, HCT116, and SW480 colon cancer cells in a dose- and time-dependent manner. The apoptosis induction was evidenced by DNA fragmentation and subG1 accumulation, which was associated with increased Bax/Bcl-2 ratio, activation of caspase-3 and cleavage of PARP. By using Tcf-dependent luciferase activity assay, HS7 was found to inhibit theβ-catenin/Tcf transcriptional activities. In addition, HS7 strongly suppressed the binding of Tcf complexes to its DNA-binding site shown in electrophoretic mobility shift assay. This inhibition was further confirmed by the decreased protein levels of Tcf-4 andβ-catenin. Theβ-catenin/Tcf downstream target genes, such assurvivin,c-myc,cyclin D1,MMP7, andMT1-MMPinvolved in apoptosis, invasion, and angiogenesis were also diminished as well. These results indicate thatTaiwanofungus camphoratusmay provide a benefit as integrative medicine for the treatment of colon cancer.

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