Characterization of Quorum Sensing and Quorum Quenching Soil Bacteria Isolated from Malaysian Tropical Montane Forest

N-Acylhomoserine lactones (AHLs) are used as quorum-sensing signals in Gram-negative bacteria. Many genes encoding AHL-degrading enzymes have been cloned and characterized in various microorganisms. Coagulase-negative staphylococci (CNS) are present on the skin of animals and are considered low-virulent species. The AHL-lactonase gene homologue, ahlS, was present in the genomes of the CNS strains Staphylococcus carnosus, Staphylococcus haemolyticus, Staphylococcus saprophyticus, and Staphylococcus sciuri. We cloned the candidate ahlS homologue from six CNS strains into the pBBR1MCS5 vector. AhlS from the CNS strains showed a higher degrading activity against AHLs with short acyl chains compared to those with long acyl chains. AhlS from S. sciuri was expressed and purified as a maltose-binding protein (MBP) fusion. Pseudomonas aeruginosa is an opportunistic pathogen that regulates several virulence factors such as elastase and pyocyanin by quorum-sensing systems. When MBP-AhlS was added to the culture of P. aeruginosa PAO1, pyocyanin production and elastase activity were substantially reduced compared to those in untreated PAO1. These results demonstrate that the AHL-degrading activity of AhlS from the CNS strains can inhibit quorum sensing in P. aeruginosa PAO1.

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Characterization of a Phosphotriesterase-Like Lactonase fromSulfolobus solfataricusand Its Immobilization for Disruption of Quorum Sensing

Applied and Environmental Microbiology ◽

10.1128/aem.01642-10 ◽

2010 ◽

Vol 77 (4) ◽

pp. 1181-1186 ◽

Cited By ~ 36

Author(s):

Filomena S. W. Ng ◽

Daniel M. Wright ◽

Stephen Y. K. Seah

Keyword(s):

Quorum Sensing ◽

Virulence Factors ◽

Acyl Chain ◽

Quorum Quenching ◽

Homoserine Lactone ◽

Homoserine Lactones ◽

Specificity Constant ◽

Chain Lengths ◽

Quorum Sensing Signals

ABSTRACTSsoPox, a bifunctional enzyme with organophosphate hydrolase andN-acyl homoserine lactonase activities from the hyperthermophilic archaeonSulfolobus solfataricus, was overexpressed and purified from recombinantPseudomonas putidaKT2440 with a yield of 9.4 mg of protein per liter of culture. The enzyme has a preference forN-acyl homoserine lactones (AHLs) with acyl chain lengths of at least 8 carbon atoms, mainly due to lowerKmvalues for these substrates. The highest specificity constant obtained was forN-3-oxo-decanoyl homoserine lactone (kcat/Km= 5.5 × 103M−1·s−1), but SsoPox can also degradeN-butyryl homoserine lactone (C4-HSL) andN-oxo-dodecanoyl homoserine lactone (oxo-C12-HSL), which are important for quorum sensing in ourPseudomonas aeruginosamodel system. WhenP. aeruginosaPAO1 cultures were grown in the presence of SsoPox-immobilized membranes, the production of C4-HSL- and oxo-C12-HSL-regulated virulence factors, elastase, protease, and pyocyanin were significantly reduced. This is the first demonstration that immobilized quorum-quenching enzymes can be used to attenuate the production of virulence factors controlled by quorum-sensing signals.

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Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia

BMC Microbiology ◽

10.1186/1471-2180-11-51 ◽

2011 ◽

Vol 11 (1) ◽

pp. 51 ◽

Cited By ~ 128

Author(s):

Kok-Gan Chan ◽

Steve Atkinson ◽

Kalai Mathee ◽

Choon-Kook Sam ◽

Siri Chhabra ◽

...

Keyword(s):

Quorum Sensing ◽

Zingiber Officinale ◽

Quorum Quenching ◽

Acylhomoserine Lactone ◽

Degrading Bacteria

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DETECTION OF QUORUM QUENCHING-PRODUCING BACTERIA FROM RHIZOSPHERE SOIL AS A BIOCONTROL AGENTS

TEKNOSAINS: MEDIA INFORMASI SAINS DAN TEKNOLOGI ◽

10.24252/teknosains.v15i1.16178 ◽

2021 ◽

Vol 15 (1) ◽

pp. 18

Author(s):

Stella Magdalena ◽

Inez Johanna ◽

Yogiara Yogiara

Keyword(s):

Quorum Sensing ◽

Soil Bacteria ◽

Bacillus Pumilus ◽

Rhizosphere Soil ◽

Quorum Quenching ◽

Biochemical Tests ◽

Gram Staining ◽

Control Gene Expression ◽

Rrna Sequencing ◽

Enterobacter Ludwigii

Quorum sensing is known as a communication mechanism among bacteria to control gene expression such as bioluminescence, pigmentation, and pathogenicity. Quorum quenching is known as inhibition of quorum sensing activity. In order to block quorum sensing activity, some bacteria produced enzymes which could degrade AHL, such as AHL-acylase, AHL-lactonase, and AHL-oxidase and reductase. In this study, soil bacteria were isolated and screened for their quorum quenching activity. These isolates were divided into Streptomyces and non-Streptomyces isolates. Detection were done by using Chromobacterium violaceum as an indicator bacteria for pigment inhibition by quorum quenching activity. About 10 out of 695 isolates were shown to possess quorum quenching activity. These isolates were further identified by biochemical tests, Gram staining, and identified by 16S rRNA sequencing. Four positive isolates showed similarity with Streptomyces sp., and 6 positive isolates were non-Streptomyces which showed similarity with Pseudomonas putida, Bacillus pumilus, Bacillus sp., Enterobacter ludwigii, and Enterobacter sp.

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