scholarly journals Aflatoxin Reduction in Maize by Industrial-Scale Cleaning Solutions

Toxins ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 331 ◽  
Author(s):  
Michelangelo Pascale ◽  
Antonio F. Logrieco ◽  
Matthias Graeber ◽  
Marina Hirschberger ◽  
Mareike Reichel ◽  
...  
Keyword(s):  
High Performance ◽  
Fine Particles ◽  
Continuous Process ◽  
Aflatoxin Contamination ◽  
Industrial Scale ◽  
Official Method ◽  
Optical Sorting ◽  
Commission Regulation ◽  
Aoac Official ◽  
Spectral Kernel

Different batches of biomass/feed quality maize contaminated by aflatoxins were processed at the industrial scale (a continuous process and separate discontinuous steps) to evaluate the effect of different cleaning solutions on toxin reduction. The investigated cleaning solutions included: (i) mechanical size separation of coarse, small and broken kernels, (ii) removal of dust/fine particles through an aspiration channel, (iii) separation of kernels based on gravity and (iv) optical sorting of spatial and spectral kernel defects. Depending on the sampled fraction, dynamic or static sampling was performed according to the Commission Regulation No. 401/2006 along the entire cleaning process lines. Aflatoxin analyses of the water–slurry aggregate samples were performed according to the AOAC Official Method No. 2005.008 based on high-performance liquid chromatography and immunoaffinity column cleanup of the extracts. A significant reduction in aflatoxin content in the cleaned products, ranging from 65% to 84% with respect to the uncleaned products, was observed when continuous cleaning lines were used. Additionally, an overall aflatoxin reduction from 55% to 94% was obtained by combining results from separate cleaning steps. High levels of aflatoxins (up to 490 µg/kg) were found in the rejected fractions, with the highest levels in dust and in the rejected fractions from the aspirator and optical sorting. This study shows that a cleaning line combining both mechanical and optical sorting technologies provides an efficient solution for reducing aflatoxin contamination in maize.

Quantitative Analysis of 5-Hydroxymethylfurfural in Linezolid Injection by High Performance Liquid Chromatography

2020 ◽  
Vol 16 (8) ◽  
pp. 1059-1067
Author(s):  
Jéssica Maurício Batista ◽  
Christian Fernandes
Keyword(s):  
High Performance ◽  
Potassium Phosphate ◽  
Gradient Elution ◽  
High Performance Liquid Chromatographic ◽  
Official Method ◽  
Ph Variation ◽  
Drug Products ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background: Linezolid is a synthetic broad-spectrum antibacterial belonging to the class of oxazolidinones. Linezolid for intravenous infusion is isotonized with dextrose. In acidic environment, the dehydration of dextrose produces furan derivatives, 5-hydroxymethylfurfural (5-HMF) being the main one. The determination of this degradation product is of fundamental importance, since there is evidence it is cytotoxic, genotoxic, mutagenic and carcinogenic. However, there is no official method for the determination of 5-HMF in drug products. Objective: The aim of this study was to develop and validate a high performance liquid chromatographic method to quantify 5-HMF in injection of linezolid. Methods: The chromatographic separation, after optimization, was performed on C18 (150 x 4.6 mm, 5 μm) column. Mobile phase was composed of 14 mM potassium phosphate buffer pH 3.0 ([H+] = 1.0 x 10-3) and methanol in gradient elution at 1.0 mL min-1. The injection volume was 10 μL and detection was performed at 285 nm. Results: The method was optimized and validated, showing selectivity, linearity in the range from 0.075 to 9.0 μg mL-1, precision (RSD ≤ 2.0%), accuracy (mean recovery of 100.07%) and robustness for temperature and pH variation. Conclusion: The method was shown to be adequate to determine 5-HMF in injection containing linezolid in routine analysis.

Adaptation of the AOAC Fluorine Method for Determining Fluoride in Fish Protein Concentrate

1970 ◽  
Vol 53 (1) ◽  
pp. 3-6
Author(s):  
R. Bruce Klemm ◽  
Mary E. Ambrose Klemm
Keyword(s):  
Steam Distillation ◽  
Silica Sand ◽  
Official Method ◽  
Protein Concentrate ◽  
Fish Protein ◽  
Direct Titration ◽  
Modified Method ◽  
Aoac Official ◽  
Aoac Official Method

Abstract The AOAC official method, 24.029–24.035, for the determination of fluorine in foods was modified slightly to o btain quantitative recoveries of fluorine from samples of fish protein concentrate (FPC). The most important alterations include the use of steam distillation, the addition of finely ground silica sand in the distillation, a decrease in the distillation temperature, and the utilization of direct titration. Recoveries of fluoride added to FPC before ashing, using this modified method, averaged 96.0 ± 3.0%. Our results are in agreement with those of several other analysts who used a variety of methods.

scholarly journals Development and certification of a reference material for zearalenone in maize germ oil

2021 ◽  
Author(s):  
Juliane Riedel ◽  
Sebastian Recknagel ◽  
Diana Sassenroth ◽  
Tatjana Mauch ◽  
Sabine Buttler ◽  
...  
Keyword(s):  
Reference Material ◽  
Reference Materials ◽  
Mass Fraction ◽  
High Performance ◽  
Certified Reference Materials ◽  
Maximum Level ◽  
Whole Process ◽  
Commission Regulation ◽  
Maize Oil ◽  
Maize Germ

AbstractZearalenone (ZEN), an estrogenic mycotoxin produced by several species of Fusarium fungi, is a common contaminant of cereal-based food worldwide. Due to frequent occurrences associated with high levels of ZEN, maize oil is a particular source of exposure. Although a European maximum level for ZEN in maize oil exists according to Commission Regulation (EC) No. 1126/2007 along with a newly developed international standard method for analysis, certified reference materials (CRM) are still not available. To overcome this lack, the first CRM for the determination of ZEN in contaminated maize germ oil (ERM®-BC715) was developed in the frame of a European Reference Materials (ERM®) project according to the requirements of ISO Guide 35. The whole process of CRM development including preparation, homogeneity and stability studies, and value assignment is presented. The assignment of the certified mass fraction was based upon an in-house study using high-performance liquid chromatography isotope dilution tandem mass spectrometry. Simultaneously, to support the in-house certification study, an interlaboratory comparison study was conducted with 13 expert laboratories using different analytical methods. The certified mass fraction and expanded uncertainty (k = 2) of ERM®-BC715 (362 ± 22) μg kg−1 ZEN are traceable to the SI. This reference material is intended for analytical quality control and contributes to the improvement of consumer protection and food safety. Graphical abstract

scholarly journals Determination of Finasteride in Tablets by High Performance Liquid Chromatography

2007 ◽  
Vol 4 (1) ◽  
pp. 109-116 ◽  
Author(s):  
K. Basavaiah ◽  
B. C. Somashekar
Keyword(s):  
High Performance ◽  
Internal Standard ◽  
High Performance Liquid Chromatographic ◽  
Reversed Phase ◽  
Calibration Graph ◽  
Official Method ◽  
Pharmaceutical Dosage Forms ◽  
Bulk Drug ◽  
Liquid Chromatographic

A rapid, highly sensitive high performance liquid chromatographic method has been developed for the determination of finasteride(FNS) in bulk drug and in tablets. FNS was eluted from a ODS C18reversed phase column at laboratory temperature (30 ± 2°C) with a mobile phase consisting of methanol and water (80+20) at a flow rate of 1 mL min-1with UV detection at 225 nm. The retention time was ∼ 6.1 min and each analysis took not more than 10 min. Quantitation was achieved by measurement of peak area without using any internal standard. Calibration graph was linear from 2.0 to 30 μg mL-1with limits of detection (LOD) and quantification (LOQ) being 0.2 and 0.6 μg mL-1, respectively. The method was validated according to the current ICH guidelines. Within-day co efficients of variation (CV) ranged from 0.31 to 0.69% and between-day CV were in the range 1.2-3.2%. Recovery of FNS from the pharmaceutical dosage forms ranged from 97.89 – 102.9 with CV of 1.41-4.13%. The developed method was compared with the official method for FNS determination in its tablet forms.

scholarly journals Towards Continuous Production of Shaped Honeycombs

2018 ◽  
Author(s):  
Sam E. Calisch ◽  
Neil A. Gershenfeld
Keyword(s):  
High Performance ◽  
Continuous Production ◽  
Continuous Process ◽  
Three Dimensional ◽  
Sandwich Panels ◽  
Performance Space ◽  
Bending Loads ◽  
Future Work ◽  
The Impact ◽  
Parallel Folding

Honeycomb sandwich panels are widely used for high performance parts subject to bending loads, but their manufacturing costs remain high. In particular, for parts with non-flat, non-uniform geometry, honeycombs must be machined or thermoformed with great care and expense. The ability to produce shaped honeycombs would allow sandwich panels to replace monolithic parts in a number of high performance, space-constrained applications, while also providing new areas of research for structural optimization, distributed sensing and actuation, and on-site production of infrastructure. Previous work has shown methods of directly producing shaped honeycombs by cutting and folding flat sheets of material. This research extends these methods by demonstrating work towards a continuous process for the cutting and folding steps of this process. An algorithm for producing a manufacturable cut-and-fold pattern from a three-dimensional volume is designed, and a machine for automatically performing the required cutting and parallel folding is proposed and prototyped. The accuracy of the creases placed by this machine is characterized and the impact of creasing order is demonstrated. Finally, a prototype part is produced and future work is sketched towards full process automation.

scholarly journals Akumulasi dan Depurasi Toksin PSP (Paralytic Shellfish Poisoning) oleh Kerang Hijau (Accumulation and Depuration of PSP Toxin (Paralytic Shellfish Poisoning) by Green Mussels)

2014 ◽  
Vol 19 (1) ◽  
pp. 27
Author(s):  
Haryoto Kusnoputranto ◽  
Setyo S Moersidik ◽  
Djarot S Wisnubroto ◽  
Murdahayu Makmur
Keyword(s):  
Paralytic Shellfish Poisoning ◽  
Perna Viridis ◽  
Official Method ◽  
Shellfish Poisoning ◽  
Toxin Concentration ◽  
Green Mussel ◽  
Mussel Farming ◽  
Paralytic Shellfish ◽  
Aoac Official ◽  
Aoac Official Method

Ledakan mikroalga sering dilaporkan terjadi di Teluk Jakarta, dimana di lokasi tersebut juga terdapat kegiatan budidaya kerang hijau (Perna viridis). Terkait dengan hal tersebut maka dilakukan studi akumulasi dan depurasi toksin PSP (Paralytic Shellfish Poisoning) pada kerang hijau. Studi akumulasi dilakukan di bagan kerang hijau perairan Cilincing Jakarta Utara, dengan memisahkan kerang hijau yang berukuran sama dan ditempatkan kembali ke bagan. Sampling dilakukan setiap minggu selama 2 bulan dan diukur juga kelimpahan fitoplankton, pH, suhu dan salinitas perairan. Depurasi dilakukan di Unit Depurasi Kekerangan KKP Panimbang Banten, yang dilakukan selama 24 jam. Pencuplikan  sampel dilakukan setiap jam pada 4 jam pertama dan setiap 2 dan 3 jam pada waktu berikutnya. Penentuan konsentrasi toksin PSP dilakukan dengan menggunakan HPLC detektor fluoresensi. Prosedur preparasi, ekstraksi dan pengukuran konsentrasi toksin mengikuti Manual AOAC Official Method 2005.06 untuk toksin PSP dalam kekerangan. Akumulasi toksin PSP oleh kerang hijau di perairan Cilincing pada bulan Januari–Pebruari 2011 berkisar antara 4,11–11,96 µg STX eq. per 100 g dan tidak mempunyai korelasi dengan kelimpahan Dinoflagelata di perairan. Hal ini disebabkan uji akumulasi tidak dilakukan pada saat blooming mikroalga. Uji depurasi selama 24 jam mengeliminasi toksin PSP sebesar 60%, sehingga bisa diajukan sebagai sistem pemutus rantai toksin dari mikroalga ke manusia. Kata kunci: akumulasi, depurasi, PSP toksin, kerang hijau, Cilincing Microalgae blooms have been frequently reported in the Jakarta Bay, which is also the location of green mussel (Perna viridis) aquaculture. Accumulation and depuration of Paralytic Shellfish Poisoning (PSP) toxin in the green mussels were investigated in the field, where the toxin accumulation studies conducted in the mussel farming at Cilincing, North Jakarta. Accumulation test carried out by placing back the selected green mussel (equal size) into the mussel farming. Every week for 2 months, the green mussel were collected from mussel farming and transported to the laboratory. The fitoplankton abundance also was checked including pH, Suhue and salinitiy paramaters. Toxin depuration was conducted at Clams Sanitation Unit at Panimbang Banten. The depuration studies were conducted for 24 hours with sampling every hour in the first 4 hours and every 3 and 2 hours until the 24th hour. Preparation, extraction and toxin concentration measurements performed by following the Manual AOAC Official Method 2005.06 for PSP toxin in oyster. This research concluded that the accumulation of PSP toxin by green mussel, Perna viridis in the mussel farming at Cilincing, North Jakarta in ranged between 4,11–11,96 µg STX eq. per 100 g during January–February 2011. No correlation between PSP toxin concentration in the green mussel, Perna viridis with abundance of the PSP toxin sources phytoplankton, because the study wasnt done when microalgae blooming. The depuration processes was eliminate 60% the PSP toxins for 24 hours depuration processing. It can be proposes as a banded system the PSP toxin from algae to human being. Keywords: accumulation, depuration, PSP toxin, green mussel, Cilincing

Allyl Isothiocyanate in Mustard Seed

1970 ◽  
Vol 53 (1) ◽  
pp. 1-3 ◽  
Author(s):  
Donald L Andersen
Keyword(s):  
Allyl Isothiocyanate ◽  
Mustard Seed ◽  
Official Method ◽  
Average Recovery ◽  
Aoac Method ◽  
Aoac Official ◽  
Mustard Seeds ◽  
Column Preparation ◽  
Aoac Official Method

Abstract A new GLC method for the determination of allyl isothiocyanate in mustard seed was compared to a method of the Midwest Research Institute and to a combination of the AOAC official method and the proposed method. Twelve collaborators compared the AOAC method and the GLC method, using whole mustard seeds. Each collaborator assayed three seed portions by both methods. The range, standard deviation, and coefficient of variation are less for each seed portion by the proposed than by the official method. The average recovery value of allyl isothiocyanate in the prepared standard solutions is lower, using the proposed GLC procedure, but seed assay values are significantly and consistently higher for each seed portion when compared with the results for the AOAC method. Reports from the collaborators also indicate that the proposed method is rugged, as the GLC column preparation was subjected to many changes. It is recommended that the GLC method be adopted as official first action.

scholarly journals Microwave Assisted-Hydrothermal Synthesis of Nickel Ferrite Nanoparticles

2018 ◽  
Vol 34 (5) ◽  
pp. 2577-2582
Author(s):  
Mohamed H. H. Mahmoud ◽  
Mahmoud M. Hessien
Keyword(s):  
Nickel Ferrite ◽  
High Performance ◽  
Fine Particles ◽  
Low Cost ◽  
Nickel Chloride ◽  
Heating Time ◽  
Stoichiometric Ratio ◽  
Microwave Assisted ◽  
Xrd Patterns ◽  
Co Precipitation

Nanomagnetic ferrite materials are of great technological importance in several industries due to their high performance, ease of preparation and low cost. The ferrite properties are based on composition, structure and methods of preparation. Nickel ferrite, NiFe2O4, was prepared by the simple microwave assisted-hydrothermal method. Nickel chloride and ferric chloride solutions (stoichiometric ratio of 1: 2 respectively) were mixed, the pH was raised to 10.5 and the mixture was heated at 180 °C in a closed Teflon vessel using a microwave oven at different periods of time (2 - 24 h). The formed powders were examined by XRD, TEM, and VSM. The intensity of nickel-ferrite in the XRD patterns increased with time owing to increase in crystallinity of the formed phase. The TEM images showed that, the size was in the range of 20-40 nm and contents of fine particles noticeably decreased with increasing reaction time to 4-6 hrs and contents of more regular cubic particles are formed. The NiFe2O4 magnetization was continuesly increased with raising the heating time from 2h (9 emu/g) to 24 h (43 emu/g) which may be due to the high purity and crystallinity of the formed NiFe2O4. The results showed that the properties of the formed ferrite can be tailored by controlling the heating time. Microwave assisted co-precipitation followed by hydrothermal digestion resulted in a substance of good homogeneity and crystallinity at a short time.

Aflatoxins in peanut oil: food safety concerns

2018 ◽  
Vol 11 (1) ◽  
pp. 149-158 ◽  
Author(s):  
G.S. Shephard
Keyword(s):  
Food Safety ◽  
Fluorescence Detection ◽  
High Performance ◽  
Developing World ◽  
Degradation Products ◽  
Hplc Method ◽  
Peanut Oil ◽  
Official Method ◽  
Safety Concerns ◽  
Wide Range

Aflatoxins are widely recognised as important natural contaminants of a wide range of foods, including maize and peanuts (groundnuts), which form part of the staple diet in many countries of the developing world, especially in Africa. There is a frequent misconception based on solubility considerations and developed market surveys that aflatoxins do not occur in peanut oil. Thus, the use of peanut oil in human food is frequently overlooked as a source of aflatoxin exposure, yet artisanal oil extraction from contaminated peanuts in local facilities in the developing world results in carryover of these mycotoxins into the oil. Consequently, these peanut oils can have high contamination levels. This review highlights food safety concerns and addresses inter alia the analytical adaptations required to determine the polar aflatoxins in peanut oil. The determination of aflatoxins in peanut oil was first achieved by thin-layer chromatography, which was later mostly superseded by high-performance liquid chromatography (HPLC) with fluorescence detection, or later, by mass spectrometric detection. More recently, a specially modified HPLC method with immunoaffinity column clean-up and fluorescence detection has achieved official method status at AOAC International. In addition, the review deals with toxicology, occurrence and detoxification of contaminated oil. Although various methods have been reported for detoxification of peanut oil, the toxicity of degradation products, the removal of beneficial constituents and the effect on its organoleptic properties need to be considered. This review is intended to draw attention to this often overlooked area of food safety.

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