scholarly journals Quantitative Measurement of Melittin in Asian Honeybee Venom Using a New Method Including UPLC-QqTOF-MS

Toxins ◽  
2020 ◽  
Vol 12 (7) ◽  
pp. 437
Author(s):  
Sheng Huang ◽  
Jianhua Wang ◽  
Zeqin Guo ◽  
Yan Wang ◽  
Chundong Liu
Keyword(s):  
Dry Weight ◽  
Ultra Performance Liquid Chromatography ◽  
Limit Of Quantification ◽  
Honeybee Venom ◽  
Flight Mass Spectrometry ◽  
Quantitative Analyses ◽  
Main Component ◽  
Asian Honeybee ◽  
Dynamic Linear Range

Asian honeybee venom is widely used in traditional oriental medicine. Melittin is the main component of Asian honeybee venom. In the present study, an ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QqTOF-MS) method was used for accurate qualitative and quantitative analyses of melittin in Asian honeybee venom. The results showed that the dynamic linear range of melittin was from 0.094 to 20 μg/mL, and the limit of quantification was 0.3125 μg/mL. The spiking recovery of melittin in honeybee venom ranged from 84.88% to 93.05%. Eighteen Asian honeybee venom samples in eighteen batches were collected from two different zones of China, and their melittin contents were measured. The contents of melittin in Asian honeybee venom samples was 33.9–46.23% of dry weight. This method proved a useful tool for the rapid evaluation of the authenticity and quality of Asian honeybee venom in terms of the melittin contents, and will contribute to a broader understanding of Asian honeybee venom.

scholarly journals Determination of ramipril in human plasma and its fragmentation by UPLC-Q-TOF-MS with positive electrospray ionization

2015 ◽  
Vol 65 (2) ◽  
pp. 159-169 ◽  
Author(s):  
Paweł Szpot ◽  
Grzegorz Buszewicz
Keyword(s):  
Electrospray Ionization ◽  
Human Plasma ◽  
Ultra Performance Liquid Chromatography ◽  
High Accuracy ◽  
Coefficient Of Determination ◽  
Limit Of Quantification ◽  
Flight Mass Spectrometry ◽  
Tof Ms ◽  
Chemical Formulas

Abstract This report presents the application of ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry with positive electrospray ionization, to determine ramipril in human plasma. First, the proteins in human plasma were precipitated using acetonitrile, then the supernatant was extracted by ethyl acetate at pH 3 and finally, the extract was analyzed using a UPLC-QTOF- MS system. The method was validated and the coefficient of determination (R2) was > 0.999, the lower limit of quantification (LLOQ) was 0.5 ng mL-1. Precision, recovery and stability were determined for three different concentrations of ramipril. RSD for this method ranged from 3.3 to 8.6 %. The intra-day mean recovery was from 65.3 to 97.3 %. In addition, the fragmentation of ramipril was studied. Due to high resolution of the spectrometer, it was possible to measure fragment masses accurately and determine their molecular and chemical formulas with high accuracy.

scholarly journals Characterization of Saponins from Various Parts of Platycodon grandiflorum Using UPLC-QToF/MS

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 107
Author(s):  
So-Jeong Lee ◽  
Heon-Woong Kim ◽  
Suji Lee ◽  
Ryeong Ha Kwon ◽  
Hyemin Na ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Total Content ◽  
Dry Weight ◽  
Acid Group ◽  
Ultra Performance Liquid Chromatography ◽  
Human Consumption ◽  
Platycodon Grandiflorum ◽  
The Core ◽  
Flight Mass Spectrometry

Platycodon grandiflorum (PG) is known as a high-potential material in terms of its biological activity. The objective of this report is to provide chromatographic and mass fragment ion data of 38 simultaneously identified saponins, including novel compounds, by analyzing them through ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-QToF/MS). In so doing, we investigated their diverse conditions, including morphological parts (stems, roots, buds, and leaves), peeling (or not), and blanching of PG. The total contents of individual saponins indicated an order of roots (containing peel, 1674.60 mg/100 g, dry weight) > buds (1364.05) > roots (without peel, 1058.83) ≈ blanched roots (without peel, 945.17) ≈ stems (993.71) ≈ leaves (881.16). When considering three types of aglycone, the platycodigenin group (55.04 ~ 68.34%) accounted for the largest proportion of the total content, whereas the platycogenic acid A group accounted for 17.83 ~ 22.61%, and the polygalacic acid group represented 12.06 ~ 22.35%. As they are classified as major compounds, novel saponins might be utilized for their role in healthy food for human consumption. Additionally, during blanching, the core temperature of PG was satisfied with the optimal condition, thus activating the enzymes related to biotransformation. Furthermore, through the use of this comprehensive data, additional studies related to buds, as well as roots or the characterization of individual saponins, can be conducted in a rapid and achievable manner.

scholarly journals Correlation between Quality and Geographical Origins of Poria cocos Revealed by Qualitative Fingerprint Profiling and Quantitative Determination of Triterpenoid Acids

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2200 ◽  
Author(s):  
Li-Xia Zhu ◽  
Jun Xu ◽  
Ru-Jing Wang ◽  
Hong-Xiang Li ◽  
Yu-Zhu Tan ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Multiple Reaction Monitoring ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Ultra Performance Liquid Chromatography ◽  
Poria Cocos ◽  
Flight Mass Spectrometry ◽  
Geographical Regions ◽  
Array Detection

Poria cocos (Schw.) Wolf (PC) is a well-known saprophytic fungus, and its sclerotium without the epidermis (PCS) is widely used in traditional Chinese medicine and as a functional food in many countries. PCS is normally collected from multiple geographical regions, but whether and how the quality of PCS correlates with where it grows have not been determined. This correlation could be significant both for quality control and optimum utilization of PCS as a natural resource. In this study, a qualitative fingerprint profiling method performed by ultra-performance liquid chromatography (UHPLC) with diode array detection (DAD) combining quadrupole time-of-flight-mass spectrometry (QTOF-MS/MS) and a quantitative UHPLC coupled with triple quadrupole mass spectrometry (QqQ-MS/MS) approach were established to investigate whether and how the quality of PCS correlates with its collection location. A standard fingerprint of PCS was generated by median simulation of 25 tested samples collected from four main producing areas of China, and similarity analysis was applied to evaluate the similarities between the fingerprints of samples and the standard fingerprint. Twenty three common peaks occurring in the fingerprint were unequivocally or tentatively identified by UHPLC-QTOF-MS/MS. Meanwhile, principal component analysis (PCA), supervised orthogonal partial least squares-discriminate analysis (OPLS-DA) and hierarchical cluster analysis (HCA) were employed to classify 25 batches of PCS samples into four groups, which were highly consistent with the four geographical regions. Ten compounds were screened out as potential markers to distinguish the quality of PCS. Nine triterpene acids, including five compounds that played important roles in the clusters between different samples collected from the four collection locations, were simultaneously quantified by using the multiple reaction monitoring (MRM) mode of UHPLC-QqQ-MS/MS. The current strategy not only clearly expounded the correlation between quality and geographical origins of PCS, but also provided a fast, accurate and comprehensive qualitative and quantitative method for assessing the quality of PCS.

scholarly journals UPLC-QTOF/MS-Based Metabolomics Applied for the Quality Evaluation of Four Processed Panax ginseng Products

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 2062 ◽  
Author(s):  
Jae Lee ◽  
Seung-Heon Ji ◽  
Bo-Ram Choi ◽  
Doo Choi ◽  
Yeong-Geun Lee ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Quality Evaluation ◽  
Principal Component ◽  
Ultra Performance Liquid Chromatography ◽  
Flight Mass Spectrometry ◽  
Black Ginseng ◽  
White Ginseng ◽  
Well Differentiated ◽  
And Control

In the food industry and herbal markets, it is critical to control the quality of processed Panax ginseng products. In this study, ultra-performance liquid chromatography coupled to quadrupole time of flight mass spectrometry (UPLC-QTOF/MS)-based metabolomics was applied for the quality evaluation of white ginseng (WG), tae-geuk ginseng (TG), red ginseng (RG), and black ginseng (BG). Diverse metabolites including ginsenosides were profiled by UPLC-QTOF/MS, and the datasets of WG, TG, RG, and BG were then subjected to multivariate analyses. In principal component analysis (PCA), four processed ginseng products were well-differentiated, and several ginsenosides were identified as major components of each product. S-plot also characterized the metabolic changes between two processed ginseng products, and the major ginsenosides of each product were found as follows: WG (M-Rb1, M-Rb2, M-Rc, Re, Rg1), TG (Rb2, Rc, Rd, Re, Rg1), RG (Rb1, Rb2, Rc, Rd, Re, Rg1), and BG (Rd, Rk1, Rg5, Rg3). Furthermore, the quantitative contents of ginsenosides were evaluated from the four processed ginseng products. Finally, it was indicated that the proposed metabolomics approach was useful for the quality evaluation and control of processed ginseng products.

scholarly journals Qualitative and semi-quantitative assessment of anthocyanins in Tibetan hulless barley from different geographical locations by UPLC-QTOF-MS and their antioxidant capacities

2021 ◽  
Vol 19 (1) ◽  
pp. 1-8
Author(s):  
Tangwei Zhang ◽  
Jialin Ma ◽  
Xuelian Wu ◽  
Zhihua Hao ◽  
Ci Dun ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Quantitative Assessment ◽  
Ultra Performance Liquid Chromatography ◽  
Total Anthocyanin ◽  
Hulless Barley ◽  
Flight Mass Spectrometry ◽  
Antioxidant Capacities ◽  
Tibetan Hulless Barley ◽  
Geographical Locations

Abstract Tibetan hulless barley (“qingke” in Chinese) is a valuable food in Tibet. Purple qingke (PQK) and black qingke (BQK), two special pigmented types of hulless barley, have traditionally been widely cultivated and consumed in Tibet for thousands of years. The composition and contents of anthocyanins of two cultivars are unknown. This study aimed to explore the composition and contents of anthocyanins of two cultivars and their antioxidant capacities. Six anthocyanins were identified by ultra-performance liquid chromatography-quadrupole-time of flight mass spectrometry from barley, most of the anthocyanins were acylated by malonyl group. The total anthocyanin contents ranged from 141 to 2,304 μg/g in PQK and from 248.7 to 2902.9 μg/g in BQK. Furthermore, qingke has strong antioxidant activity against DPPH, ABTS˙+, and FRAP. Qingke may be useful for treating or preventing diseases caused by the overproduction of radicals.

scholarly journals Supranational Assessment of the Quality of Probiotics: Collaborative Initiative between Independent Accredited Testing Laboratories

2021 ◽  
Vol 9 (7) ◽  
pp. 1456
Author(s):  
Jean-Pol Warzée ◽  
Marina Elli ◽  
Abdoulaye Fall ◽  
Daniela Cattivelli ◽  
Jean-Yves François
Keyword(s):  
Daily Basis ◽  
Qualitative Assessment ◽  
Close Collaboration ◽  
Quality Label ◽  
Accredited Laboratories ◽  
Quantitative Analyses ◽  
Quality Of Food ◽  
The Eu

Recent acquisitions about the role of the microbiota in the functioning of the human body make it possible to envisage an increasing use of beneficial microbes, and more particularly of probiotics as well as their metabolites, as nutritional supplements. National and EU authorities are engaged in assuring the safety and quality of food supplements and in defining rules to assess and communicate their efficacy on human health. The quality of probiotics, intended as strains’ identification, viability, and stability over time, is a crucial factor of credibility with consumers and health professionals. Analytical technologies for the quality control of probiotics must also be adapted to new preparations, such as those including new multistrains complex combinations. Accredited laboratories face this relevant challenge on a daily basis. Through its close collaboration with the laboratory commissioned to produce the specifications for its ESLP quality label (identification and quantitative analyses) together with its scientific committee, the ESLP has been focusing on this issue for 10 years. Recently, as part of the internationalization of the ESLP quality label, a new and unique initiative in Europe for the evaluation of the quality of probiotic preparations has been carried out. The collaboration between two accredited laboratories in Belgium and in Italy represented a concrete example of supranational collaboration in the assessment of the quality of probiotic preparations. Results show that both laboratories are in line as expected in terms of performance. Common approaches to the qualitative assessment of probiotic preparations, especially for complex and composite recipes, in terms of number of strains and included substances, should be encouraged and promoted all over the EU.

Sign in / Sign up

Export Citation Format

Share Document