Molecular Epidemiology of Human Papillomaviruses, Neisseria gonorrhoeae, Chlamydia trachomatis and Mycoplasma genitalium among Female Sex Workers in Burkina Faso: Prevalence, Coinfections and Drug Resistance Genes

Viral and bacterial infections represent an occupational risk for female sex workers. This study aimed at determining HPV coinfection with genital pathogens among female sex workers in West and Central Africa and identifying antibiotic resistance genes. A total of 182 samples from female sex workers were analyzed by real-time PCR and classic PCR. For the molecular diagnosis of HPV, the real-time multiplex amplification kit “HPV Genotypes 14 Real-TM Quant” from SACACE Biotechnologies®, detecting 14 high-risk HPV genotypes, was used, while for other pathogens, the real-time multiplex amplification kit N. gonorrhoeae/C. trachomatis/M. genitalium/T. vaginalis Real-TM, allowing their simultaneous detection, was used. The women were aged 17–50 years with an average age of 27.12 ± 6.09 years. The pathogens identified were HPV 54.94% (100/120), Neisseria gonorrhoeae (13.74%), Chlamydia trachomatis (11.54%) and Mycoplasma genitalium (11.54%). The most common HPV genotypes were HPV68, HPV38 and HPV52. The antibiotic resistance genes identified were bla QNR B 24.00%, bla GES 22.00%, bla SHV 17.00%, blaCTX-M 13.00% and bla QNR S 1.00%. This study revealed the presence of various HPV genotypes associated with other pathogens with problems of antibiotic resistance among sex workers of West and Central African origin working in Ouagadougou.

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Macrolide resistance in Mycoplasma genitalium from female sex workers in Belgium

Journal of Global Antimicrobial Resistance ◽

10.1016/j.jgar.2017.09.018 ◽

2018 ◽

Vol 12 ◽

pp. 149-152 ◽

Cited By ~ 12

Author(s):

Liselotte Coorevits ◽

Ans Traen ◽

Luk Bingé ◽

Patrick Descheemaeker ◽

Jerina Boelens ◽

...

Keyword(s):

Sex Workers ◽

Female Sex Workers ◽

Mycoplasma Genitalium ◽

Macrolide Resistance ◽

Female Sex

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Rapid detection of enterococci and antibiotic resistance genes in urine: Approaching real-time capability

Clinical Microbiology Newsletter ◽

10.1016/s0196-4399(00)88667-5 ◽

1998 ◽

Vol 20 (23) ◽

pp. 192-195 ◽

Cited By ~ 1

Author(s):

Vito G. DelVecchio ◽

Sharon Rose ◽

Joe J. Shaw

Keyword(s):

Antibiotic Resistance ◽

Real Time ◽

Resistance Genes ◽

Rapid Detection ◽

Antibiotic Resistance Genes

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Evaluation of residual antibacterial potency in antibiotic production wastewater using a real-time quantitative method

Environmental Science Processes & Impacts ◽

10.1039/c5em00228a ◽

2015 ◽

Vol 17 (11) ◽

pp. 1923-1929 ◽

Cited By ~ 10

Author(s):

Hong Zhang ◽

Yu Zhang ◽

Min Yang ◽

Miaomiao Liu

Keyword(s):

Antibiotic Resistance ◽

Real Time ◽

Environmental Impacts ◽

Resistance Genes ◽

Quantitative Method ◽

Antibiotic Production ◽

Antibiotic Resistance Genes ◽

Antibiotic Activity ◽

Wastewater Discharge ◽

Related Substances

While antibiotic pollution has attracted considerable attention due to its potential in promoting the dissemination of antibiotic resistance genes in the environment, the antibiotic activity of their related substances has been neglected, which may underestimate the environmental impacts of antibiotic wastewater discharge.

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Cervical Human Papillomavirus Prevalence, Genotypes, and Associated Risk Factors among Female Sex Workers in Greater Accra, Ghana

Journal of Oncology ◽

10.1155/2019/8062176 ◽

2019 ◽

Vol 2019 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Abdul Rashid Adams ◽

Priscillia Awo Nortey ◽

Benjamin Ansah Dortey ◽

Richard Harry Asmah ◽

Edwin Kwame Wiredu

Keyword(s):

Cervical Cancer ◽

High Risk ◽

Cancer Prevention ◽

Sex Workers ◽

Female Sex Workers ◽

Cervical Cancer Prevention ◽

Human Papillomaviruses ◽

Female Sex ◽

Hpv Prevalence ◽

Hpv Genotypes

Cervical cancer is a largely preventable disease mediated by persistent infection with high-risk Human Papillomaviruses (Hr-HPV). There are now three approved vaccines against the most common HPV genotypes. In Ghana, mortality due to cervical cancer is on the rise, due to the absence of an organized and effective cervical cancer prevention and control program. Data on circulating HPV genotypes is important for studying the likely impact of mass introduction of HPV vaccination of the female population before sexual debut. High HPV prevalence has been reported in Female Sex Workers (FSWs), who constitute an important active group for maintenance of HPV in the population. This study was conducted to determine the size of HPV prevalence in this group and to provide information for future assessment of the impact of vaccine introduction in the country. We conducted a cross-sectional study where the snowballing technique was used to identify and select FSW’s ≥18 years, operating within suburbs of Greater Accra Region (GAR). A risk factor assessment interview was conducted and cervical swabs were collected for HPV-DNA detection and genotyping by Nested Multiplex PCR. Hundred participants, age ranging from 18 to 45 years, median 24 years, were studied. The prevalence of Cervical HPV was 26%. Eleven genotypes were detected comprising 9 high-risk in order of decreasing prevalence HPV-16 (8%), HPV-35 (5%), HPV-33/39/-68 (3%), HPV-52/51/59 (2%) and HPV-18 (1%) and 2 Low-risk types, HPV-42(3%), and HPV-43 (1%). Three women had HPV types that could not be genotyped by our method. Oral contraceptives use was associated with a reduced chance of HPV infection (P=0.002; OR=0.19, 95% CI 0.07-0.54). This study found a high HPV prevalence among FSWs in the GAR. A high number of Hr-HPV genotypes seen are vaccine preventable, providing additional compelling argument for implementing a national cervical cancer prevention plan including vaccination.

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Antibiotic Resistance Genes in the Bacteriophage DNA Fraction of Human Fecal Samples

Antimicrobial Agents and Chemotherapy ◽

10.1128/aac.01684-13 ◽

2013 ◽

Vol 58 (1) ◽

pp. 606-609 ◽

Cited By ~ 63

Author(s):

Pablo Quirós ◽

Marta Colomer-Lluch ◽

Alexandre Martínez-Castillo ◽

Elisenda Miró ◽

Marc Argente ◽

...

Keyword(s):

Antibiotic Resistance ◽

Real Time ◽

Quantitative Pcr ◽

Resistance Genes ◽

Antibiotic Resistance Genes ◽

Fecal Samples ◽

Real Time Quantitative Pcr ◽

Healthy Humans ◽

Phage Dna

ABSTRACTA group of antibiotic resistance genes (ARGs) (blaTEM,blaCTX-M-1,mecA,armA,qnrA, andqnrS) were analyzed by real-time quantitative PCR (qPCR) in bacteriophage DNA isolated from feces from 80 healthy humans. Seventy-seven percent of the samples were positive in phage DNA for one or more ARGs.blaTEM,qnrA, and,blaCTX-M-1were the most abundant, andarmA,qnrS, andmecAwere less prevalent. Free bacteriophages carrying ARGs may contribute to the mobilization of ARGs in intra- and extraintestinal environments.

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Prevalence and Correlates of Mycoplasma genitalium Infection Among Female Sex Workers in Kampala, Uganda

The Journal of Infectious Diseases ◽

10.1093/infdis/jir733 ◽

2011 ◽

Vol 205 (2) ◽

pp. 289-296 ◽

Cited By ~ 35

Author(s):

Judith Vandepitte ◽

Etienne Muller ◽

Justine Bukenya ◽

Susan Nakubulwa ◽

Nassim Kyakuwa ◽

...

Keyword(s):

Sex Workers ◽

Female Sex Workers ◽

Mycoplasma Genitalium ◽

Female Sex

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The Transferable Resistome of Produce

mBio ◽

10.1128/mbio.01300-18 ◽

2018 ◽

Vol 9 (6) ◽

Cited By ~ 26

Author(s):

Khald Blau ◽

Antje Bettermann ◽

Sven Jechalke ◽

Eva Fornefeld ◽

Yann Vanrobaeys ◽

...

Keyword(s):

Antibiotic Resistance ◽

Real Time ◽

Resistance Genes ◽

Real Time Pcr ◽

Direct Detection ◽

Blot Hybridization ◽

Antibiotic Resistance Genes ◽

Human Pathogens ◽

Content Type ◽

E Coli

ABSTRACTProduce is increasingly recognized as a reservoir of human pathogens and transferable antibiotic resistance genes. This study aimed to explore methods to characterize the transferable resistome of bacteria associated with produce. Mixed salad, arugula, and cilantro purchased from supermarkets in Germany were analyzed by means of cultivation- and DNA-based methods. Before and after a nonselective enrichment step, tetracycline (TET)-resistantEscherichia coliwere isolated and plasmids conferring TET resistance were captured by exogenous plasmid isolation. TET-resistantE. coliisolates, transconjugants, and total community DNA (TC-DNA) from the microbial fraction detached from leaves or after enrichment were analyzed for the presence of resistance genes, class 1 integrons, and various plasmids by real-time PCR and PCR-Southern blot hybridization. Real-time PCR primers were developed for IncI and IncF plasmids. TET-resistantE. coliisolated from arugula and cilantro carried IncF, IncI1, IncN, IncHI1, IncU, and IncX1 plasmids. Three isolates from cilantro were positive for IncN plasmids andblaCTX-M-1. From mixed salad and cilantro, IncF, IncI1, and IncP-1β plasmids were captured exogenously. Importantly, whereas direct detection of IncI and IncF plasmids in TC-DNA failed, these plasmids became detectable in DNA extracted from enrichment cultures. This confirms that cultivation-independent DNA-based methods are not always sufficiently sensitive to detect the transferable resistome in the rare microbiome. In summary, this study showed that an impressive diversity of self-transmissible multiple resistance plasmids was detected in bacteria associated with produce that is consumed raw, and exogenous capturing intoE. colisuggests that they could transfer to gut bacteria as well.IMPORTANCEProduce is one of the most popular food commodities. Unfortunately, leafy greens can be a reservoir of transferable antibiotic resistance genes. We found that IncF and IncI plasmids were the most prevalent plasmid types inE. coliisolates from produce. This study highlights the importance of the rare microbiome associated with produce as a source of antibiotic resistance genes that might escape cultivation-independent detection, yet may be transferred to human pathogens or commensals.

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