scholarly journals Characterization of the Mycovirome from the Plant-Pathogenic Fungus Cercospora beticola

Viruses ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1915
Author(s):  
Yingxi Li ◽  
Mengke Zhou ◽  
Yizhou Yang ◽  
Qi Liu ◽  
Zongying Zhang ◽  
...  
Keyword(s):  
Sugar Beet ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Pathogenic Fungus ◽  
High Yield ◽  
Cercospora Beticola ◽  
Sequencing Analysis ◽  
Cercospora Leaf Spot ◽  
Potential Biocontrol Agent ◽  
Production Areas

Cercospora leaf spot (CLS) caused by Cercospora beticola is a devastating foliar disease of sugar beet (Beta vulgaris), resulting in high yield losses worldwide. Mycoviruses are widespread fungi viruses and can be used as a potential biocontrol agent for fugal disease management. To determine the presence of mycoviruses in C. beticola, high-throughput sequencing analysis was used to determine the diversity of mycoviruses in 139 C. beticola isolates collected from major sugar beet production areas in China. The high-throughput sequencing reads were assembled and searched against the NCBI database using BLASTn and BLASTx. The results showed that the obtained 93 contigs were derived from eight novel mycoviruses, which were grouped into 3 distinct lineages, belonging to the families Hypoviridae, Narnaviridae and Botourmiaviridae, as well as some unclassified (−)ssRNA viruses in the order Bunyavirales and Mononegavirales. To the best of our knowledge, this is the first identification of highly diverse mycoviruses in C. beticola. The novel mycoviruses explored in this study will provide new viral materials to biocontrol Cercospora diseases. Future studies of these mycoviruses will aim to assess the roles of each mycovirus in biological function of C. beticola in the future.

scholarly journals High-Throughput Sequencing Analysis of the Actinobacterial Spatial Diversity in Moonmilk Deposits

Antibiotics ◽  
2018 ◽  
Vol 7 (2) ◽  
pp. 27 ◽  
Author(s):  
Marta Maciejewska ◽  
Magdalena Całusińska ◽  
Luc Cornet ◽  
Delphine Adam ◽  
Igor Pessi ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Spatial Diversity ◽  
Sequencing Analysis

In vitro fertilization and preimplantation genetic testing methods in infertility treatment of a woman with karyotype 46,XX,ins(13;4)(q34;p14p15.3),inv(4)(p14q12). Case report

GYNECOLOGY ◽  
2021 ◽  
Vol 23 (5) ◽  
pp. 441-444
Author(s):  
Zhanna I. Glinkina ◽  
Elena V. Kulakova ◽  
Elena G. Lebedeva ◽  
Varvara S. Kuzmicheva ◽  
Nataliya P. Makarova
Keyword(s):  
Genetic Testing ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Chromosomal Abnormalities ◽  
Reproductive Technologies ◽  
Infertility Treatment ◽  
Preimplantation Embryos ◽  
Sequencing Analysis ◽  
Preimplantation Genetic Testing ◽  
And Inversion

The frequency of structural chromosomal transpositions can range from 1.8 to 8% among patients with reproductive disorders. There are several types of the rarest chromosomal abnormalities: insertion (insertion of a chromosomal region) and inversion (rotation of a chromosome region). This article describes a clinical case of the infertility treatment using assisted reproductive technologies in a woman with a rare chromosomal abnormality: simultaneous insertion and inversion of chromosomes 46, XX, ins (13;4)(q34;p14p15.3), inv(4)(p14q12). The structure and frequency of chromosomal aberrations were determined by high-throughput sequencing in preimplantation embryos. The result of the sequencing analysis showed that unbalanced variants for a known pathology were detected in 9 (56.3%) out of 16 observations, while in 6 (37%) only for a pathology known in the karyotype and in 3 (19%) they were presented simultaneously with the pathology of other chromosomes or with mosaicism. According to the results of the study, in preimplantation embryos, where one of the parents had chromosomal abnormalities, in addition to unbalanced variants, there is aneuploidy of other chromosomes not involved in the known pathology. They are described in 3 (21%) out of 14 observations of all identified pathology. In this regard, patients with aberrations in the karyotype are recommended, whenever possible, to carry out preimplantation genetic testing of structural rearrangements by methods allowed to analyze all chromosomes simultaneously. For example, high-throughput sequencing on the Illumina platform may become an alternative for prenatal diagnostics, which is performed in fertile couples with high risk of having a child with hereditary or congenital disorders. In the case of detection of chromosomal changes in the fetus, patients are faced with a number of ethical issues related to the necessity for medical abortion, which may contradict their religious and moral convictions.

High-throughput sequencing analysis of endophytic fungal diversity in cynanchum sp.

2020 ◽  
Vol 134 ◽  
pp. 349-358
Author(s):  
W.-H. Chen ◽  
S.-J. Wu ◽  
X.-L. Sun ◽  
K.-M. Feng ◽  
K. Rahman ◽  
...  
Keyword(s):  
High Throughput ◽  
Fungal Diversity ◽  
High Throughput Sequencing ◽  
Sequencing Analysis

scholarly journals High-throughput sequencing analysis of intestinal flora changes in ESRD and CKD patients

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Jianguang Hu ◽  
Xiaoshi Zhong ◽  
Jing Yan ◽  
Daoyuan Zhou ◽  
Danping Qin ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Intestinal Flora ◽  
Sequencing Analysis

scholarly journals Assessment of endophytic bacterial diversity in rose by high-throughput sequencing analysis

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0230924
Author(s):  
Ao-Nan Xia ◽  
Jun Liu ◽  
Da-Cheng Kang ◽  
Hai-Guang Zhang ◽  
Ru-Hua Zhang ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
High Throughput ◽  
High Throughput Sequencing ◽  
Sequencing Analysis
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