scholarly journals Oral Delivery of Probiotics Expressing Dendritic Cell-Targeting Peptide Fused with Porcine Epidemic Diarrhea Virus COE Antigen: A Promising Vaccine Strategy against PEDV

Viruses ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 312 ◽  
Author(s):  
Xiaona Wang ◽  
Li Wang ◽  
Xuewei Huang ◽  
Sunting Ma ◽  
Meiling Yu ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Porcine Epidemic Diarrhea Virus ◽  
Oral Delivery ◽  
Cell Targeting ◽  
Vaccine Strategy ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea ◽  
Antigen A ◽  
Targeting Peptide

scholarly journals Construction and immunogenicity analysis of Lactobacillus plantarum expressing a porcine epidemic diarrhea virus S gene fused to a DC-targeting peptide

2018 ◽  
Vol 247 ◽  
pp. 84-93 ◽  
Author(s):  
Ke-Yan Huang ◽  
Gui-Lian Yang ◽  
Yu-Bei Jin ◽  
Jing Liu ◽  
Hong-Liang Chen ◽  
...  
Keyword(s):  
Lactobacillus Plantarum ◽  
Porcine Epidemic Diarrhea Virus ◽  
Diarrhea Virus ◽  
S Gene ◽  
Porcine Epidemic Diarrhea ◽  
Targeting Peptide

scholarly journals Immune Responses in Pregnant Sows Induced by Recombinant Lactobacillus johnsonii Expressing the COE Protein of Porcine Epidemic Diarrhea Virus Provide Protection for Piglets against PEDV Infection

Viruses ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 7
Author(s):  
Dianzhong Zheng ◽  
Xiaona Wang ◽  
Ning Ju ◽  
Zhaorui Wang ◽  
Ling Sui ◽  
...  
Keyword(s):  
Dendritic Cell ◽  
Immune Responses ◽  
Porcine Epidemic Diarrhea Virus ◽  
Oral Vaccine ◽  
Economic Losses ◽  
Antigen Delivery ◽  
Lactobacillus Johnsonii ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

Porcine epidemic diarrhea (PED) induced by porcine epidemic diarrhea virus (PEDV) is an intestinal infectious disease in pigs that causes serious economic losses to the pig industry. To develop an effective oral vaccine against PEDV infection, we used a swine-origin Lactobacillus johnsonii (L. johnsonii) as an antigen delivery carrier. A recombinant strain pPG-T7g10-COE/L. johnsonii (L. johnsonii-COE) expressing COE protein (a neutralizing epitope of the viral spike protein) was generated. The immunomodulatory effect on dendritic cell in vitro and immunogenicity in pregnant sows was evaluated following oral administration. L. johnsonii-COE could activate monocyte-derived dendritic cell (MoDC) maturation and triggered cell immune responses. After oral vaccination with L. johnsonii-COE, levels of anti-PEDV-specific serum IgG, IgA, and IgM antibodies as well as mucosal secretory immunoglobulin A (SIgA) antibody were induced in pregnant sows. High levels of PEDV-specific SIgA and IgG antibodies were detected in the maternal milk, which provide effective protection for the piglets against PEDV infection. In summary, oral L. johnsonii-COE was able to efficiently activate anti-PEDV humoral and cellular immune responses, demonstrating potential as a vaccine for use in sows to provide protection of their piglets against PEDV.

scholarly journals Oral delivery of maize-produced porcine epidemic diarrhea virus spike protein elicits neutralizing antibodies in pigs

2020 ◽  
Vol 142 (1) ◽  
pp. 79-86
Author(s):  
Erin Egelkrout ◽  
Celine Hayden ◽  
Gina Fake ◽  
Todd Keener ◽  
Paulo Arruda ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Oral Delivery ◽  
Neutralizing Antibodies ◽  
Spike Protein ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Cell attenuated porcine epidemic diarrhea virus strain Zhejiang08 provides effective immune protection attributed to dendritic cell stimulation

Vaccine ◽  
2017 ◽  
Vol 35 (50) ◽  
pp. 7033-7041 ◽  
Author(s):  
Yuchen Li ◽  
Guihua Wang ◽  
Jialu Wang ◽  
Kun Man ◽  
Qian Yang
Keyword(s):  
Dendritic Cell ◽  
Virus Strain ◽  
Porcine Epidemic Diarrhea Virus ◽  
Immune Protection ◽  
Cell Stimulation ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

scholarly journals Characteristics and Pathogenicity of the Cell-Adapted Attenuated Porcine Epidemic Diarrhea Virus of the Non-S INDEL Cluster

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1479
Author(s):  
Thi Thu Hang Vu ◽  
Minjoo Yeom ◽  
Hyoungjoon Moon ◽  
Thi Nhan Tran ◽  
Van Phan Le ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
High Titer ◽  
Vaccine Strategy ◽  
Live Attenuated Vaccine ◽  
Virus Shedding ◽  
Diarrhea Virus ◽  
Attenuated Vaccine ◽  
In Vivo Tests ◽  
Porcine Epidemic Diarrhea

The high antigenic diversity of porcine epidemic diarrhea virus (PEDV) means that porcine epidemic diarrhea (PED) is a challenge for the global pig industry. Understanding the circulation of the virus to determine an optimal vaccine strategy is important in controlling the disease. In this study, we describe the genetic diversity of circulating PEDV based on the full sequences of spike genes of eight positive samples collected in Vietnam since 2018. Additionally, we developed a live attenuated vaccine candidate from the cell-adapted PEDV2 strain, which was continuously passaged until level 103 in VERO-CCL81 cells. PEDV2-p103, which belongs to the emerging non-S INDEL cluster, exhibited low virus shedding, did not induce lesions in the small intestine of challenged piglets, and had a high titer in the VERO-CCL81 cell at 48 h post-infection. These results suggest that the PEDV2-p103 strain could be a potential oral attenuated vaccine, and its immunogenicity and efficacy should be further assessed through in vivo tests.

scholarly journals Identifying outbreaks of Porcine Epidemic Diarrhea virus through animal movements and spatial neighborhoods

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Gustavo Machado ◽  
Carles Vilalta ◽  
Mariana Recamonde-Mendoza ◽  
Cesar Corzo ◽  
Montserrat Torremorell ◽  
...  
Keyword(s):  
Porcine Epidemic Diarrhea Virus ◽  
Animal Movements ◽  
Diarrhea Virus ◽  
Porcine Epidemic Diarrhea

PSVI-4 Efficacy of Medium Chain Fatty Acids and Fatty Acid-based Feed Additives as a Mitigation Strategy Against Porcine Epidemic Diarrhea Virus and Porcine Reproductive and Respiratory Syndrome Virus

2021 ◽  
Vol 99 (Supplement_1) ◽  
pp. 216-217
Author(s):  
O L Harrison ◽  
G E Nichols ◽  
J T Gebhardt ◽  
Cassandra K Jones ◽  
Jason C Woodworth ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Porcine Epidemic Diarrhea Virus ◽  
Positive Control ◽  
Feed Additives ◽  
Feed Additive ◽  
Respiratory Syndrome Virus ◽  
Post Inoculation ◽  
Diarrhea Virus ◽  
Medium Chain ◽  
Porcine Epidemic Diarrhea

Abstract Recent research has demonstrated that swine viruses can be transmitted via feed. Chemical feed additives have been suggested for the mitigation of these viruses in complete feed. Therefore, the objective of this study was to evaluate the efficacy of a commercially available formaldehyde-based feed additive, medium chain fatty acid blend (MCFA), and commercially available fatty acid-based products for mitigation of porcine epidemic diarrhea virus (PEDV) and porcine reproductive and respiratory syndrome virus (PRRSV) in a feed matrix. Treatments consisted of: 1) non-treated positive control, 2) 0.33% commercial formaldehyde-based product (Sal Curb; Kemin Industries, Inc.; Des Moines, IA), 3) 0.5% MCFA blend (1:1:1 ratio of C6:0, C8:0, and C10:0, Sigma Aldrich, St. Louis, MO), 4) 0.25%, 5) 0.5%, or 6) 1% of commercial dry mono and diglyceride-based product (Furst Strike; Furst-McNess Company, Freeport, IL), 7) 0.25%, 8) 0.5%, or 9) 1% of commercial dry mono and diglyceride-based product (Furst Protect; Furst-McNess Company, Freeport, IL), 10) 0.25%, 11) 0.5%, or 12) 1% dry mono and diglyceride-based experimental product (Furst-McNess Company, Freeport, IL) with 3 replications/treatment. Treatments were applied to complete swine feed before inoculation with 106 TCID50/g of feed with PEDV or PRRSV. Post inoculation feed was held at ambient temperature for 24 h before being analyzed via qRT-PCR. The analyzed values represent the cycle threshold. Formaldehyde and MCFA decreased (P < 0.05) the detectable RNA of PEDV and PRRSV compared to all other treatments. Furst Strike, Furst Protect, and the experimental product did not significantly impact detectability of PEDV or PRRSV RNA. In conclusion, MCFA and formaldehyde treatments are effective at reducing detection of RNA from PEDV and PRRSV in feed.

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