СРАВНИТЕЛЬНОЕ ОПРЕДЕЛЕНИЕ СОДЕРЖАНИЯ ОБЩЕГО БЕЛКА В СЫВОРОТКЕ КРОВИ КОРОВ БИОХИМИЧЕСКИМ И ТЕНЗИОМЕТРИЧЕСКИМ МЕТОДАМИ

2020 ◽  
Author(s):  
S.YU. ZAITSEV
Keyword(s):  
Surface Tension ◽  
Blood Serum ◽  
Total Protein ◽  
Biological Fluids ◽  
Dynamic Surface Tension ◽  
Total Protein Content ◽  
Serum Samples ◽  
Dynamic Surface ◽  
Biochemical Methods ◽  
Air Interface

Основой метода межфазной тензиометрии является измерение динамического поверхностного натяжения (ДПН) биологических жидкостей человека и животных. В данной работе предложено использование метода регрессии в моделировании взаимосвязи биохимических и ДПН параметров сыворотки крови коров. Показана возможность определения количества общего белка в сыворотке крови телок по известным параметрам тензиограмм поверхностного натяжения на границе раздела жидкость/воздух. В результате измерений 96 проб сыворотки крови телок трех возрастов (6, 12 и 1718 мес) и обработки полученных данных были определены следующие диапазоны значений ДПН: 072,173,9 мН/м, 171,973,5 мН/м, 266,870,1 мН/м, 361,264,4 мН/м, 03,165,96 мНм-1с-1/2, 15,707,34 мНм-1с-1/2. При включении полученных экспериментальных данных ДПН в новое регрессионное уравнение получены следующие значения содержания общего белка: 72,4 г/л 66,7 г/л 69,5 г/л (для телок 6, 12 и 1718 мес), соответственно. Эти значения на 2,5 выше 1,8 и 1,4 ниже, чем соответствующие значения общего белка в сыворотке крови телок, измеренные традиционными биохимическими методами.Данная модель для показателя общего белка имеет хорошее качество (P0,05) и готова к использованию. Регрессионный анализ с использованием простого экспресс-метода межфазной тензиометрии позволяет избавиться от необходимости постоянных измерений показателей общего белка в сыворотке крови телок биохимическими методами.The basis of the method of interfacial tensiometry is the measurement of the dynamic surface tension (DST) of biological fluids in humans and animals. In this paper, the application of the regression method in modeling the relationship of biochemical and DST parameters of cow blood serum is proposed. The possibility of determining the amount of total protein in the blood serum of heifers by the known parameters of tensiograms of surface tension at the liquid/air interface is shown. As a result of measurements of the 96 blood serum samples of heifers of three ages (6, 12 and 1718 months) and processing the obtained data, the following ranges of DST values were determined: 072.173.9 mN/m, 171.973.5 mN/m, 266.870.1 mN/m, 361.264.4 mN/m, 03.165.96 mNm-1s-1/2, 15.707.34 mNm-1s-1/2. When checking the obtained new regression equation according to the serum DST, the following values of the total protein content were obtained: 72.4 g/l 66.7 g/l 69.5 g/l (for heifers of 6, 12 and 1718 months), respectively. These values are about 2.5 higher, 1.8 and 1.4 lower than the corresponding total protein values measured by the traditional biochemical methods. Thus, this model for the indicator of total protein is of good quality (P0.05) and is ready for use. Regression analysis using a simple express-method of interfacial tensiometry allows you to eliminate the need for continuous measurements of total protein in the blood serum of heifers by biochemical methods.

Determination of albumin content in blood serum based on the results of dynamic surface tension

2021 ◽  
Vol 1 (6) ◽  
pp. 68-73
Author(s):  
M. S. Tsarkova ◽  
◽  
I. V. Milaeva ◽  
S. Yu. Zaytsev ◽  
◽  
...  
Keyword(s):  
Surface Tension ◽  
Blood Serum ◽  
Objective Assessment ◽  
Dynamic Surface Tension ◽  
The Body ◽  
Maximum Pressure ◽  
Dynamic Surface ◽  
Good Convergence ◽  
Albumin Content

The blood test allows you to give an objective assessment of the state of health of animals and timely identify changes occurring in the body. To assess the content of albumins in the blood serum, the method of measuring the dynamic surface tension on the VRA-1P device, which works according to the method of maximum pressure in the bubble, was used. Based on the results of the measurements, a mathematical model was proposed, and using the regression analysis method, formulas for determining the concentration of albumins were developed, which showed good convergence with other measurement methods.

scholarly journals Properties of the seawater-air interface. Dynamic surface tension studies1

1979 ◽  
Vol 24 (6) ◽  
pp. 1022-1030 ◽  
Author(s):  
Dj. Dragcevic ◽  
M. Vukovic ◽  
D. Cukman ◽  
V. Pravdic
Keyword(s):  
Surface Tension ◽  
Dynamic Surface Tension ◽  
Dynamic Surface ◽  
Air Interface ◽  
Interface Dynamic

scholarly journals Superficial tension of blood and urine in diabetic nephropathy

1998 ◽  
Vol 44 (3) ◽  
pp. 7-10
Author(s):  
O. V. Sinyachenko ◽  
V. N. Kazakov ◽  
V. B. Faynerman ◽  
R. Miller ◽  
N. D. Cholangot ◽  
...  
Keyword(s):  
Surface Tension ◽  
Nephrotic Syndrome ◽  
Diabetic Nephropathy ◽  
Biological Fluids ◽  
Dynamic Surface Tension ◽  
Type I ◽  
Type Ii ◽  
Dynamic Surface ◽  
Maximal Pressure ◽  
Serum And Urine

Dynamic surface tension (ST) of the blood serum and urine was studied in patients with diabetic nephropathy by the method of maximal pressure in a vesicle using MPT2 computer tensiometer (LAUDA, Germany). The angle of the slope of blood tensiogram curve increased and urinary ST decreased at t = 1 sec in patients with type I diabetics in comparison with those with type II condition. The development of the nephrotic syndrome and chronic renal insufficiency was associated with decrease of ST in the range of short (t = 0.01 sec) and medium (t = 1 sec) times of surface life and of the equilibrium (static) ST (at t -> go/. The parameters of interphase tensiometry correlated with the content of protein and lipid surfactants in biological fluids.

scholarly journals Determination of the Stabilization Time of the Solution-Air Interface for Aggregates Formed by NaC in Mixtures with SDS and PEO, Investigated by Dynamic Surface Tension Measurements

Soft ◽  
2014 ◽  
Vol 03 (01) ◽  
pp. 1-10 ◽  
Author(s):  
Grasiele Raupp ◽  
Arlindo C. Felippe ◽  
Tiago Elias Allievi Frizon ◽  
Luciano da Silva ◽  
Marcos Marques da Silva Paula ◽  
...  
Keyword(s):  
Surface Tension ◽  
Dynamic Surface Tension ◽  
Stabilization Time ◽  
Dynamic Surface ◽  
Air Interface

Dynamic Surface Tension and Adsorption Kinetics of β-Casein at the Solution/Air Interface

Langmuir ◽  
2004 ◽  
Vol 20 (3) ◽  
pp. 771-777 ◽  
Author(s):  
R. Miller ◽  
V. B. Fainerman ◽  
E. V. Aksenenko ◽  
M. E. Leser ◽  
M. Michel
Keyword(s):  
Surface Tension ◽  
Adsorption Kinetics ◽  
Dynamic Surface Tension ◽  
Dynamic Surface ◽  
Air Interface ◽  
Kinetics Of

scholarly journals Methodological approach to the tensiometrical analysis of the blood serum samples of Duroc pigs

2021 ◽  
Vol 2052 (1) ◽  
pp. 012003
Author(s):  
S L Belopukhov ◽  
S Yu Zaitsev
Keyword(s):  
Blood Serum ◽  
Methodological Approach ◽  
Dynamic Surface Tension ◽  
Serum Samples ◽  
Dynamic Surface ◽  
Data Set ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Very High

Abstract The aim of this work was to compare the dynamic surface tension (DST) with biochemical parameters of the blood serum of Duroc pigs (BSDP). BSDP samples were taken after various times of pig fattening: 65 days (Group 1), 72 days (Group 2), 84-89 days (Group 3), 91-100 days (Group 4). Our approach is consisting in the BSDP-DST-measurement using all four modes of BPA-tensiometer: M1) “Standard experiment”; M2) “Experiment at constant lifetime”; M3) “Accelerated experiment”; M4) “Quick scan”. Here, we have worked out all the modes mentioned above for determining the BSDP tensiometric parameters. The BSDP-DST parameters obtained for groups 3 and 4 (84-89 and 91-101 fattening days, respectively) were very high and close in numbers. (from 73.4-74.6 mN/m for STo, to 58.1-60.5 mN/m for STm, respectively). These BSDP-DST parameters were significantly higher (7-13%) at extremely low interface “life-times” (0.01-0.1 s), as compared to those for groups 1 and 2 (65 and 72 fattening days, respectively). In contrast, the BSDP-DST parameters obtained for groups 1 and 2 were low (from 66.1-71.4 mN/m for STo, to 56.9-57.9 mN/m for STm, respectively). These data (in combination with other physical-chemical methods) can be used for the fundamental data set and monitoring the pig growth, health, productivity, etc.

Dynamic surface tension of xylem sap at the air-water interface

2021 ◽  
Author(s):  
Reddy Prasanna Duggireddy ◽  
Eran Raveh ◽  
Gilboa Arye
Keyword(s):  
Surface Tension ◽  
Surface Activity ◽  
Pure Water ◽  
Xylem Sap ◽  
Dynamic Surface Tension ◽  
Bulk Solution ◽  
Diffusion Control ◽  
Dynamic Surface ◽  
Equilibrium Surface Tension ◽  
Air Interface

<p>The surface tension (ST) of xylem sap at the water-air interface is a crucial phenomenon, influencing many physiological events such as air seeding and embolism, by which xylem vessels become air-filled and cease to function. Refilling of embolized, may relies on sap’s surface activity at the interface. It is commonly assumed that the ST of xylem sap is equal to the ST of pure water (72 mN/m). However, xylem sap is a complex solution and consists of surface-active molecules that may adsorb and accumulate at the water-air interface and thereby reduce the ST of water as a function of their aqueous concentration. However, when a new water-air interface is formed, equilibrium ST is not reached instantaneously. Specifically, amphiphilic molecules are kinetically adsorbed and undergo orientation at the interface following diffusion from the bulk solution. Dynamic ST of xylem sap and liquid-solid interactions, describing the surface phenomena of the xylem of vascular plants is currently not fully understood. This is mainly due to a lack of quantitative knowledge on the rate and extent of dynamic and equilibrium ST of sap. In this regard, the main objective of this study is to quantify the dynamic and equilibrium ST of xylem sap as a function of their aqueous concentration. We extracted xylem sap from lemon trees and measured ST as a function of time using the pendant drop technique. The dynamic ST data were analyzed using empirical and diffusion-control mathematical models which adequately described the exponential-like decay of the ST as a function of time. The results showed reduced ST of water in the xylem sap, indicating significant surface activity, reaching equilibrium ST values as low as 42 mN/m. The rate of ST decay was higher in high sap concentration and reduced in diluted one. The results of dynamic and equilibrium ST and the corresponding model will be presented and their implications for xylem hydraulic functioning will be discussed.</p><p> </p><p>Keywords: Dynamic surface tension, Equilibrium surface tension, Diffusion, Xylem sap.</p><p> </p>

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