scholarly journals Methodological approach to the tensiometrical analysis of the blood serum samples of Duroc pigs

2021 ◽  
Vol 2052 (1) ◽  
pp. 012003
Author(s):  
S L Belopukhov ◽  
S Yu Zaitsev
Keyword(s):  
Blood Serum ◽  
Methodological Approach ◽  
Dynamic Surface Tension ◽  
Serum Samples ◽  
Dynamic Surface ◽  
Data Set ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Very High

Abstract The aim of this work was to compare the dynamic surface tension (DST) with biochemical parameters of the blood serum of Duroc pigs (BSDP). BSDP samples were taken after various times of pig fattening: 65 days (Group 1), 72 days (Group 2), 84-89 days (Group 3), 91-100 days (Group 4). Our approach is consisting in the BSDP-DST-measurement using all four modes of BPA-tensiometer: M1) “Standard experiment”; M2) “Experiment at constant lifetime”; M3) “Accelerated experiment”; M4) “Quick scan”. Here, we have worked out all the modes mentioned above for determining the BSDP tensiometric parameters. The BSDP-DST parameters obtained for groups 3 and 4 (84-89 and 91-101 fattening days, respectively) were very high and close in numbers. (from 73.4-74.6 mN/m for STo, to 58.1-60.5 mN/m for STm, respectively). These BSDP-DST parameters were significantly higher (7-13%) at extremely low interface “life-times” (0.01-0.1 s), as compared to those for groups 1 and 2 (65 and 72 fattening days, respectively). In contrast, the BSDP-DST parameters obtained for groups 1 and 2 were low (from 66.1-71.4 mN/m for STo, to 56.9-57.9 mN/m for STm, respectively). These data (in combination with other physical-chemical methods) can be used for the fundamental data set and monitoring the pig growth, health, productivity, etc.

scholarly journals EPIZOOTOLOGICAL AND EPIDEMIOLOGICAL ZONING OF THE TERRITORY OF NORTH CAUCASUS FEDERAL REGION BY A DEGREE OF NON-WELFARE BY ANTHRAX USING ARC GIS10 PROGRAM

2016 ◽  
pp. 19-24 ◽  
Author(s):  
N. P. Buravtseva ◽  
S. N. Antyuganov ◽  
O. V. Semenko ◽  
A. G. Ryazanova ◽  
E. I. Eremenko ◽  
...  
Keyword(s):  
North Caucasus ◽  
Group 4 ◽  
Low Degree ◽  
Stavropol Region ◽  
Group 3 ◽  
Group 2 ◽  
High Degree ◽  
Sporadic Infection ◽  
North Ossetia ◽  
Very High

Aim. Conducting epizootological and epidemiological zoning ofthe territory of North Caucasus Federal Region (NCFR) by the degree of non-welfare by anthrax using Arc GIS10 program. Materials and methods. 2 parameters were used during zoning of the territories of subjects of NCFR (Stavropol Region, Karachaevo-Cherkessia, Kabardino-Balkaria, Chechen, North Ossetia-Alania, Ingushetia, Dagestan Republics) by non-welfare by anthrax: integrated parameter of infection risk and epizootics risk. The parameters were input into the Arc GIS10 program. Materials on infection of humans and animals with anthrax for the last 55 (1960 - 2014) years were used. Results. The regions of the territories of NCFR subjects were distributed by non-welfare degree into 4 groups: group 1 - regions with a low degree of non-welfare, group 2 - regions with an average degree of non-welfare, group 3 - regions with a high degree of non-welfare, group 4 - regions with a very high degree of non-welfare. Conclusion. Taking into account high parameters of spread and intensity of epizootological and epidemiological situation, regions of groups 3 and 4 could be classified as endemic territories for anthrax, whereas all the other regions could be attributed to the zone of sporadic infection manifestation.

scholarly journals Quantitative PCR-Enhanced Immunoassay for Measurement of Enteroviral Immunoglobulin M Antibody and Diagnosis of Aseptic Meningitis

2005 ◽  
Vol 12 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Amal Elfaitouri ◽  
Nahla Mohamed ◽  
Jan Fohlman ◽  
Robert Aspholm ◽  
Gun Frisk ◽  
...  
Keyword(s):  
Aseptic Meningitis ◽  
Solid Phase ◽  
Immunoglobulin M ◽  
Serum Samples ◽  
Serum Dilution ◽  
Group 4 ◽  
Control Serum ◽  
Group 3 ◽  
Group 2 ◽  
Capture Technique

ABSTRACT A PCR-enhanced immunoassay (PIA) to detect enterovirus (EV) immunoglobulin M (IgM) for diagnosis of recent EV infection was recently developed. This test was compared with another EV IgM capture technique, the solid-phase reverse immunosorbent test (SPRIST). Fourteen of 43 serum samples from aseptic meningitis patients were positive by PIA, whereas 10 were positive by SPRIST. One of 39 control serum samples was weakly positive by PIA. A single-serum-dilution real-time PCR-based PIA for EV IgM (quantitative PIA [QPIA]) was also developed and evaluated against PIA, SPRIST, an EV IgM radioimmunoassay (RIA), and clinical data. A mixture of 12 EVs was used as the antigen. Results from investigating four groups of serum samples were as follows. (i) The nine PIA-positive serum samples in group 1 were all positive by QPIA. (ii) Group 2 consisted of 59 serum samples from aseptic meningitis patients. Nineteen of 30 serum samples (63%) taken at hospital admission were positive by QPIA. Of these, 17 were positive in EV PCR. (iii) None of the 30 control serum samples in group 3 were positive by QPIA. (iv) For the 24 serum samples in group 4, of which 11 were positive and 13 were negative by RIA, the QPIA results were completely concordant. The sensitivity and specificity of QPIA for diagnosis of EV infection were 70 and 80%, respectively. QPIA provides a rational strategy for the detection of EV IgM, allows the use of viral antigens with minimal purification, and needs no virus-specific reagents apart from those in the PCR. QPIA is a generally applicable method for the detection of viral IgM in IgM capture assays.

Autumnal timing of photoperiodic manipulation critical via melatonin to winter pelage development in mink

1995 ◽  
Vol 61 (3) ◽  
pp. 589-596 ◽  
Author(s):  
M. Valtonen ◽  
O. Vakkuri ◽  
L. Blomstedt
Keyword(s):  
Hair Follicles ◽  
Night Time ◽  
Group 4 ◽  
Natural Photoperiod ◽  
Weight Loss Group ◽  
Group 3 ◽  
Group 2 ◽  
Long Photoperiod ◽  
Melatonin Production ◽  
Very High

AbstractQuantitive collection of night-time urine every 2nd week was used to elucidate the temporal relationship between changes in melatonin production, weight gain, and priming of the winter pelage under natural light conditions and under a long photoperiod (18L: 6D) imposed at different times in four groups of female mink during autumn. In mink maintained outdoors under the natural photoperiod (group 1), melatonin excretion was 1·80 (s.d. 0·80) ng per night (no. = 3) at the beginning of September, thereafter it began to decrease slowly until November when it was only 0·62 (s.d. 0·32) ng per night. These mink had a normal autumn moult during the first half of October and the winter pelage was mature at the end of November. When mink maintained outdoors were transferred to 18L: 6D on 7 September (group 2), excretion of melatonin decreased promptly, autumn moult was disrupted and winter fur priming delayed. Long photoperiod after mid October (group 3), did not interfere with pelage maturation despite the decrease in melatonin production. Mink implanted with melatonin on 14 July (group 4) showed very high melatonin excretion in early September. In these animals the winter pelage growth was unaffected by the long-day conditions; the pelage matured in mid October. Body weight increased in mink of all groups during autumn. This increase levelled off or a weight loss (group 2) was seen along with the final hair maturation. Activation of hair follicles occurred during a 4 to 6 week period. According to these results, melatonin is the photoperiodic signal to autumnal weight increase and autumn moult but seems not to be necessary for later pelage growth and maturation.

СРАВНИТЕЛЬНОЕ ОПРЕДЕЛЕНИЕ СОДЕРЖАНИЯ ОБЩЕГО БЕЛКА В СЫВОРОТКЕ КРОВИ КОРОВ БИОХИМИЧЕСКИМ И ТЕНЗИОМЕТРИЧЕСКИМ МЕТОДАМИ

2020 ◽  
Author(s):  
S.YU. ZAITSEV
Keyword(s):  
Surface Tension ◽  
Blood Serum ◽  
Total Protein ◽  
Biological Fluids ◽  
Dynamic Surface Tension ◽  
Total Protein Content ◽  
Serum Samples ◽  
Dynamic Surface ◽  
Biochemical Methods ◽  
Air Interface

Основой метода межфазной тензиометрии является измерение динамического поверхностного натяжения (ДПН) биологических жидкостей человека и животных. В данной работе предложено использование метода регрессии в моделировании взаимосвязи биохимических и ДПН параметров сыворотки крови коров. Показана возможность определения количества общего белка в сыворотке крови телок по известным параметрам тензиограмм поверхностного натяжения на границе раздела жидкость/воздух. В результате измерений 96 проб сыворотки крови телок трех возрастов (6, 12 и 1718 мес) и обработки полученных данных были определены следующие диапазоны значений ДПН: 072,173,9 мН/м, 171,973,5 мН/м, 266,870,1 мН/м, 361,264,4 мН/м, 03,165,96 мНм-1с-1/2, 15,707,34 мНм-1с-1/2. При включении полученных экспериментальных данных ДПН в новое регрессионное уравнение получены следующие значения содержания общего белка: 72,4 г/л 66,7 г/л 69,5 г/л (для телок 6, 12 и 1718 мес), соответственно. Эти значения на 2,5 выше 1,8 и 1,4 ниже, чем соответствующие значения общего белка в сыворотке крови телок, измеренные традиционными биохимическими методами.Данная модель для показателя общего белка имеет хорошее качество (P0,05) и готова к использованию. Регрессионный анализ с использованием простого экспресс-метода межфазной тензиометрии позволяет избавиться от необходимости постоянных измерений показателей общего белка в сыворотке крови телок биохимическими методами.The basis of the method of interfacial tensiometry is the measurement of the dynamic surface tension (DST) of biological fluids in humans and animals. In this paper, the application of the regression method in modeling the relationship of biochemical and DST parameters of cow blood serum is proposed. The possibility of determining the amount of total protein in the blood serum of heifers by the known parameters of tensiograms of surface tension at the liquid/air interface is shown. As a result of measurements of the 96 blood serum samples of heifers of three ages (6, 12 and 1718 months) and processing the obtained data, the following ranges of DST values were determined: 072.173.9 mN/m, 171.973.5 mN/m, 266.870.1 mN/m, 361.264.4 mN/m, 03.165.96 mNm-1s-1/2, 15.707.34 mNm-1s-1/2. When checking the obtained new regression equation according to the serum DST, the following values of the total protein content were obtained: 72.4 g/l 66.7 g/l 69.5 g/l (for heifers of 6, 12 and 1718 months), respectively. These values are about 2.5 higher, 1.8 and 1.4 lower than the corresponding total protein values measured by the traditional biochemical methods. Thus, this model for the indicator of total protein is of good quality (P0.05) and is ready for use. Regression analysis using a simple express-method of interfacial tensiometry allows you to eliminate the need for continuous measurements of total protein in the blood serum of heifers by biochemical methods.

Morphological Changes in the Rat Granulosa Cell Surface During Differentiation Induced by Estradiol and Gonadotropins

1977 ◽  
Vol 35 ◽  
pp. 484-485
Author(s):  
P. Bagavandoss ◽  
JoAnne S. Richards ◽  
A. Rees Midgley
Keyword(s):  
Cell Surface ◽  
Granulosa Cell ◽  
Morphological Changes ◽  
Follicular Development ◽  
Female Rats ◽  
Functional Changes ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2

During follicular development in the mammalian ovary, several functional changes occur in the granulosa cells in response to steroid hormones and gonadotropins (1,2). In particular, marked changes in the content of membrane-associated receptors for the gonadotropins have been observed (1).We report here scanning electron microscope observations of morphological changes that occur on the granulosa cell surface in response to the administration of estradiol, human follicle stimulating hormone (hFSH), and human chorionic gonadotropin (hCG).Immature female rats that were hypophysectcmized on day 24 of age were treated in the following manner. Group 1: control groups were injected once a day with 0.1 ml phosphate buffered saline (PBS) for 3 days; group 2: estradiol (1.5 mg/0.2 ml propylene glycol) once a day for 3 days; group 3: estradiol for 3 days followed by 2 days of hFSH (1 μg/0.1 ml) twice daily, group 4: same as in group 3; group 5: same as in group 3 with a final injection of hCG (5 IU/0.1 ml) on the fifth day.

Correlation of γ-radioactivity and Scanning Electron Microscopy in measurement of retention of 111Indium-labeled canine endothelial cells on synthetic vascular grafts

1989 ◽  
Vol 47 ◽  
pp. 886-887
Author(s):  
E.J. Prendiville ◽  
S. Laliberté Verdon ◽  
K. E. Gould ◽  
K. Ramberg ◽  
R. J. Connolly ◽  
...  
Keyword(s):  
Blood Flow ◽  
Ex Vivo ◽  
Vascular Grafts ◽  
Retention Data ◽  
Vascular Prostheses ◽  
Group 4 ◽  
Human Fibronectin ◽  
Insufficient Time ◽  
Group 3 ◽  
Group 2

Endothelial cell (EC) seeding is postulated as a mechanism of improving patency in small caliber vascular grafts. However the majority of seeded EC are lost within 24 hours of restoration of blood flow in previous canine studies . We postulate that the cells have insufficient time to fully develop their attachment to the graft surface prior to exposure to hemodynamic stress. We allowed EC to incubate on fibronectin-coated ePTFE grafts for four different time periods after seeding and measured EC retention after perfusion in a canine ex vivo shunt circuit.Autologous canine EC, were enzymatically harvested, grown to confluence, and labeled with 30 μCi 111 Indium-oxine/80 cm 2 flask. Four groups of 5 cm x 4 mm ID ePTFE vascular prostheses were coated with 1.5 μg/cm.2 human fibronectin, and seeded with 1.5 x 105 EC/ cm.2. After seeding grafts in Group 1 were incubated in complete growth medium for 90 minutes, Group 2 were incubated for 24 hours, Group 3 for 72 hours and Group 4 for 6 days. Grafts were then placed in the canine ex vivo circuit, constructed between femoral artery and vein, and subjected to blood flow of 75 ml per minute for 6 hours. Continuous counting of γ-activity was made possible by placing the seeded graft inside the γ-counter detection crystal for the duration of perfusion. EC retention data after 30 minutes, 2 hours and 6 hours of flow are shown in the table.

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

Defensive activity of Hesperidin against Ciprofloxacin induced hepatic injury in rabbits.

2019 ◽  
Vol 10 (03) ◽  
Author(s):  
Hawraa M. Murad ◽  
Tamadhur Hani Hussein ◽  
Audai Sulaiman Khudhair ◽  
Manal Muhi Murad ◽  
Jawad Kadhim Faris
Keyword(s):  
Body Weight ◽  
Bacterial Infections ◽  
Hepatoprotective Activity ◽  
The Body ◽  
Local Breed ◽  
Group 4 ◽  
Group 3 ◽  
Group 2 ◽  
Defensive Activity ◽  
Antioxidant Effects

This study was conducted to find out hepatoprotective activity of hesperidin (HES) 100mg/kg body weight (b.w.) against ciprofloxacin (CPX) 100 mg/kg induced hepatotoxicity in local breed rabbits .CPX is a broad spectrum antibiotic used for treatment of many bacterial infections. Twenty four male rabbits were divided into four groups ,group1: control, (1 ml/kg Saline orally) group 2: CPX (100 mg/kg orally) for (14) consecutive days , group 3: HES (100 mg//kg) orally for (14) consecutive days group 4: CPX (100 mg/kg orally) plus HES (100 mg//kg orally ) for (14) consecutive days. All the rabbits were killed on the (15) day of the experiment, and then the blood, and livers samples were taken. CPX induced hepatotoxicity was proved by a significant (p less than 0.01) reduction in the body weight ,and a significant (p less than 0.01) increased serum aspartate transaminase (AST), alanine transaminase (ALT) , Malonaldehyde enzyme (MAD) and histopathological changes. Protective hepatic toxicity effect and oxidative damage caused by CPX significantly (p less than 0.01) increasing in body weight and significantly (p less than 0.01) decreasing AST , ALT, MAD and improving tissue morphology in HES (100 mg//kg) . These results assure that HES (100 mg//kg) antioxidant effects can protect CPX-induced hepatotoxicity in rabbits.

Does Perioperative Hemoglobin A1c Level Affect the Incidence, Pattern and Mortality of Lower Extremity Amputation?

2019 ◽  
Vol 17 (4) ◽  
pp. 354-364
Author(s):  
Hassan Al-Thani ◽  
Moamena El-Matbouly ◽  
Maryam Al-Sulaiti ◽  
Noora Al-Thani ◽  
Mohammad Asim ◽  
...  
Keyword(s):  
Glycemic Control ◽  
Lower Extremity ◽  
Hazard Ratio ◽  
Lower Extremity Amputation ◽  
Group 4 ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Extremity Amputation ◽  
Group 1

Background: We hypothesized that perioperative HbA1c influenced the pattern and outcomes of Lower Extremity Amputation (LEA). Methods: A retrospective analysis was conducted for all patients who underwent LEA between 2000 and 2013. Patients were categorized into 5 groups according to their perioperative HbA1c values [Group 1 (<6.5%), Group 2 (6.5-7.4%), Group 3 (7.5-8.4%), Group 4 (8.5-9.4%) and Group 5 (≥9.5%)]. We identified 848 patients with LEA; perioperative HbA1c levels were available in 547 cases (Group 1: 18.8%, Group 2: 17.7%, Group 3: 15.0%, Group 4: 13.5% and Group 5: 34.9%). Major amputation was performed in 35%, 32%, 22%, 10.8% and 13.6%, respectively. Results: The overall mortality was 36.5%; of that one quarter occurred during the index hospitalization. Mortality was higher in Group 1 (57.4%) compared with Groups 2-5 (46.9%, 38.3%, 36.1% and 31.2%, respectively, p=0.001). Cox regression analysis showed that poor glycemic control (Group 4 and 5) had lower risk of mortality post-LEA [hazard ratio 0.57 (95% CI 0.35-0.93) and hazard ratio 0.46 (95% CI 0.31-0.69)]; this mortality risk persisted even after adjustment for age and sex but was statistically insignificant. The rate of LEA was greater among poor glycemic control patients; however, the mortality was higher among patients with tight control. Conclusion: The effects of HbA1c on the immediate and long-term LEA outcomes and its therapeutic implications need further investigation.

Association between cervical disc disease and lesions of multiple sclerosis

2021 ◽  
pp. 197140092098356
Author(s):  
Marwan Alkrenawi ◽  
Michael Osherov ◽  
Azaria Simonovich ◽  
Jonathan Droujin ◽  
Ron Milo ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Cervical Disc ◽  
Cervical Disc Disease ◽  
Disc Disease ◽  
Group 4 ◽  
Demyelinating Lesions ◽  
Grade Ii ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Background Cervical discopathy and demyelinating lesions often co-exist in patients with multiple sclerosis (MS). Our study examines the possible association between these two pathologies. Methods Medical records and cervical magnetic resonance imaging scans of MS patients with cervical discopathy who were seen at our MS clinic during 2018 were retrospectively reviewed. The severity of the disc disease was classified as grade I (no compression), grade II (compression of the dural sac) and grade III (cord compression). The spinal cord in each scan was divided into six segments corresponding to the intervertebral space of the spine (C1–C6). Each segment was defined as containing demyelinating lesion and disc pathology (group 1), demyelinating lesion without disc pathology (group 2), disc pathology without demyelinating lesion (group 3) and no demyelinating lesion or disc pathology (group 4). Fisher’s exact test was used to test the association between demyelinating lesions and disc pathology. Results Thirty-four MS patients with cervical discopathy were included in the study (26 females; average age 42.9 ± 13.7 years; average disease duration 8.4 ± 5.4 years). A total of 204 spinal cord segments were evaluated. Twenty-four segments were classified as group 1, 27 segments as group 2, 52 segments as group 3 and 101 segments as group 4. There was no association between demyelinating lesions and the grade of disc disease ( p = 0.1 for grade I, p = 0.3 for grade II and p = 1 for grade III disc disease). Conclusion Our study did not find any association between cervical disc disease and demyelinating spinal cord lesion.

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