scholarly journals Phytochemical constituents, antioxidant activity and antimicrobial potential of Pulicaria incisa (lam.) DC as a folk medicinal plant

2019 ◽  
Vol 2 (4) ◽  
pp. 22-227
Author(s):  
Mahinder Partap ◽  
Raghbir Chand Gupta ◽  
Saroj Kumar Pradhan

Objective: Comparative analysis of morphology and phytochemical constituents in different populations and morphotypes of Datura innoxia Mill. and Datura metel L. from Punjab plains.Methods: Morphological analyses of different populations have been done. Methanol extracts of seeds and roots of different populations have been prepared and total phenols, flavonoid contents were measured through spectrophotometry. Antioxidant activity was studied by 2, 2-diphenyl-1- picrylhydrazyl radical scavenging activity and total antioxidant capacity. Two major compounds, caffeic acid and chlorogenic acid were quantified by high-performance thin-layer chromatography (HPTLC) analyses.Results: Two morphotypes of D. metel were reported in the present study. Out of five different populations of D. metel and D. innoxia, the wild populations have more bioactive compound as compared to the cultivated ones from the detailed phytochemical investigation. Pharmacologically important two marker compounds chlorogenic acid and caffeic acid has been identified and quantified by HPTLC technique.Conclusion: Variation in terms of morphology and secondary metabolites exists among the different populations of Datura spp. Among the two plant parts studied, seeds have the maximum amount of bioactive metabolites and antioxidant activity. This study revealed that chlorogenic acid and caffeic acid are the potential polyphenolic compounds in Datura spp. It has been found that the antioxidant activity of plant is due to its polyphenol contents, which provides insight to various researchers to work on it as it imparts health benefit.


2018 ◽  
Vol 7 (1) ◽  
pp. 51-55
Author(s):  
Suman Joshi DSD ◽  
◽  
Chander Amgoth ◽  
Surya Narayana S ◽  
Padmavathi CH ◽  
...  

Herein, we report on the anticancer and antioxidant activity of an Aporphine alkaloid isolated from medicinal plant Alphonsea sclerocarpa. Conventional column chromatography and preparative HPLC methods were used to isolate and purify the alkaloid. Based on NMR, FTIR and MS/MS spectroscopic techniques the compound was identified as Crebanine. This is the first report on the presence of Crebanine from the leaves of Alphonsea sclerocarpa. Antioxidant activity was evaluated using DPPH and FRAP assay. The antioxidant activity was dose-dependent and activity increased with the increase in the concentration. The cytotoxicity assay was carried out using MTT assay. The results showed remarkable cytotoxic activity against K562 (CML-chronic myeloid Leukaemia blood cancer) cells with a % inhibition of 37.545 at 500µm concentration after an incubation of 24h


Author(s):  
Shailja Choudhary ◽  
Hemlata Kaurav ◽  
Gitika Chaudhary

Cichorium intybus is commonly known as Chicory, kasani. It is a recognized medicinal plant that belongs to the Asteraceae family. This plant is a well-known traditional herb used in various medicinal systems like Ayurveda, Unani and Siddha medicinal system to treat variety of diseases ranging from wounds to diabetes. The species is native to the European region (Mediterranean region) but can also grow in the temperate and semi-arid regions. The juice extracted from this plant is used as a traditional remedy to cure uterus cancer and tumors. The plant is well-known for its roots that are used as an additive in coffee as it provides bitterness in taste without the caffeine. It is also reported that the roots of the plant contain 40% inulin and acts as an anti-diabetic agent. Historically, the plant was cultivated by ancient Egyptians as a medicinal plant, vegetable crop, coffee substitute and also used as animal forage. Cichorium intybus contains various phytochemical constituents mainly sesquiterpene lactones, caffeic acid derivatives, inulin flavonoids, alkaloids, phenols, steroids terpenoids and many more. The reported pharmacological properties of the C. intybus plant include hepatoprotective, anti-diabetic, anti-cancerous, anti-inflammatory, analgesic, cardiovascular, antioxidant, antimicrobial, anthelmintic, antimalarial, anti-allergic and gastroprotective activities. In this review article, the medicinal and ayurvedic importance of C. intybus plant along with its phytochemicals are briefly explained.


2019 ◽  
Vol 8 (4) ◽  
pp. 48-52
Author(s):  
O. V. Trineeva

Introduction. Recently, much attention has been paid to the primary assessment of the pharmacological effect of various drugs using in vivo and in vitro tests. It is known that such a medicinal plant as sea buckthorn, in its phytochemical composition is rich in natural antioxidants: carotenoids, tocopherols, flavonoids, ascorbic acid, etc. In some publications there is information about the antioxidant activity of sea buckthorn and fatty oil based on them. However, information on the comparative characteristics of the use of various methods for determining the antioxidant activity of this type of medicinal plant material and the results obtained are not found in the scientific literature.Aim. The aim of this work was a comparative determination of the antioxidant activity of medicinal plant material of buckthorn fruits of various species of buckthorn.Materials and methods. The total antioxidant activity of water and water-alcohol extracts from the fruits of sea buckthorn fruits was determined using various techniques recommended in the literature. The antioxidant activity of the extracts was determined by permanganometric titration, in vitro inhibition of adrenaline autooxidation, and also in a biological model, Parametium caudatum cell culture.Results and discussion. The effect of the extractant polarity on the value of antioxidant activity was studied. It was found that the highest content of antioxidants in the extraction is observed when using 96 % ethanol as an extractant.Conclusion. Using three methods, the prospects of using sea buckthorn fruits and preparations based on them as a source of antioxidants are shown. 


Pharmacophore ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 1-5
Author(s):  
Nurliyana Athirah Md Sidek ◽  
Barry Van Der Berg ◽  
Khairana Husain ◽  
Mazlina Mohd Said

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