scholarly journals ANALYSIS OF PROMISING STRAWBERRY HYBRID FORMS BY FAOMT AND FAFAD1 FRUIT AROMA GENES

2021 ◽  
Vol 3 (27) ◽  
pp. 117-124
Author(s):  
A.S. Lyzhin ◽  
◽  
I.V. Luk'yanchuk ◽  
Keyword(s):  
Molecular Marker ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Gene Identification ◽  
Fragaria Ananassa ◽  
Strawberry Fruit ◽  
Biological Objects ◽  
Allelic State ◽  
Functional Alleles ◽  
Hybrid Forms

An important consumer trait of strawberry fruits is their aroma. Among the most important strawberry fruit aroma compounds are γ-decalactone and mesifurane. The content of γ-decalactone in fruits is controlled by the expression of the FaFAD1 gene. The content of mesifurane in fruits is controlled by the FaOMT gene. Identification of genotypes carrying the target alleles of the aromatic complex genes is an important stage in improving the strawberry assortment and creating strawberry varieties with aromatic fruits. The purpose of the study is molecular genetic analysis of strawberry hybrid seedlings by the FaOMT and FaFAD1 genes to identify genotypes promising in breeding for fruit aroma. Promising selected and elite forms of strawberry (Fragaria × ananassa Duch.) created at the FSSI “I.V. Michurin FSC” were used as biological objects. To identify the allelic state FaOMT gene, the codominant molecular marker FaOMT-SI/NO was used. To identify FaFAD1 gene, the molecular marker FaFAD1 was used. Using the FaOMT-SI/NO marker, the functional allele of the FaOMT gene (FaOMT+) was identified in the following strawberry selected forms: 298-19-9-43 (FB2 F. orientalis Los., F. moschata Duch., F. × ananassa Duch.), 932-29 (F. virginiana subsp. platypetala (Rydb.) Staudt × ‘Feyerverk’), 928-12 (298-19-9-43 × ‘Privlekatelnaya’), 69-29 (‘Feyerverk’× ‘Bylinnaya’), 72-25, 72-71 (‘Privlekatelnaya’ × ‘Bylinnaya’) and 26-5 (‘Rubinovyy kulon’× 298-19-9-43). The FaFAD1 gene was identified in strawberry hybrids 72-25 (‘Privlekatelnaya’ × ‘Bylinnaya’), 56-8 (‘Gigantella’ × ‘Privlekatelnaya’) and 61-12 (‘Bylinnaya’ × ‘Olimpiyskaya nadezhda’). The strawberry selected form 72-25 (‘Privlekatelnaya’ × ‘Bylinnaya’) is characterized by a combination of functional alleles of both genes (FaOMT + FaFAD1). Involvement of these forms in hybridization will make it possible to intensify the process of creating new strawberry varieties with improved fruit aroma.

scholarly journals Creation of new tomato forms with fungal disease resistance genes based on marker selection

2021 ◽  
pp. 16-21
Author(s):  
I. N. Shamshin ◽  
E. V. Grosheva ◽  
M. V. Maslova ◽  
R. M. Samoilova
Keyword(s):  
Resistance Gene ◽  
Resistance Genes ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Fungal Diseases ◽  
Heterozygous State ◽  
Standard Type ◽  
Marker Selection ◽  
Young Leaves ◽  
Hybrid Forms

Relevance. The presented studies are aimed at obtaining new forms of tomato with a complex of genes for resistance to fungal diseases in combination with a standard type of bush and dark coloring of fruits based on marker-mediated selection.Methodology. The biological objects of the study are varieties and hybrid forms of tomato from the collection of the Michurinsky SAU. Molecular genetic analysis was performed using the following methods. DNA extraction was carried out from young leaves using a kit for isolation of NC Sample NC manufactured by Agrodiagnostika LLC according to the manufacturer's protocol. Fermentas production kits were used for PCR. Identification of the cladosporosis resistance gene was Cf-19 performed using the DNA marker R7. The presence of a fusarious wilting resistance gene was determined by a I-2/5 marker. The amplification results were visualized by agarose gel electrophoresis.Results. During the research, a collection of varieties and hybrid forms of tomato of the Michurinsky GAU was analyzed in order to identify genes for resistance to cladosporiosis Cf-19 and fusarium wilt I-2. A total of 52 genotypes were analyzed. It was found that most samples (41 samples) are characterized by a heterozygous state of the Cf-19 gene. All indeterminant and semi-determinant forms had both alleles. Of the 23 determinant forms presented in the collection, 10 had only one allele corresponding to recessive homozygote. Among all analyzed tomato genotypes, no dominant homozygous forms were noted. The study of the collection revealed several alleles of the I-2 gene. In total, four fragments corresponding to various alleles were amplified. A total of 50 resistant genotypes have been identified in the collection. Two alleys of the I-2 gene (633/693 bp) were identified in 42 tomato samples. Four varieties are homozygous in one allele (633 bp), which determines resistance. Three varieties have a second resistance allele (566 bp). One genotype has only an allele defining susceptibility (693 bp). On the basis of molecular analysis, as well as an assessment of the type of bush and fetal color, initial forms were selected with subsequent hybridization. 67 hybrid tomato plants were obtained. Evaluation of the presence of resistance genes showed that most of the resulting hybrids are resistant to cladosporiosis and fuzariosis. This is due to the presence of dominant alleles of Cf-19 and I-2 genes in a heterozygous state. Among the resulting hybrids, plants with a bark type of bush were identified. A total of 13 such plants were obtained.Conclusion. Thus, the work carried out allowed to obtain hybrid forms of tomato combine the signs of resistance to two pathogens of fungal diseases and the stem type of the bush. These forms are planned to be used in further selection work.

scholarly journals Determination of allelic variants of gene Glu-1 in hybrid families, bearing gene Gpc-B1 of Triticum turgidum ssp. dicoccoides

1970 ◽  
Vol 21 ◽  
pp. 168-173
Author(s):  
S. Yu. Pokhylko ◽  
A. I. Stepanenko ◽  
O. M. Dugan ◽  
B. V. Morgun
Keyword(s):  
Triticum Turgidum ◽  
Molecular Genetic ◽  
Triticum Aestivum L ◽  
Molecular Genetic Analysis ◽  
Kernel Weight ◽  
Allelic Variants ◽  
Allelic State ◽  
Soft Winter Wheat ◽  
And Performance

Aim. The aim of our study was to analyze the hybrid families of generation F5 carrying gene Gpc-B1 of Triticum turgidum ssp. dicoccoides for the allelic state of Glu-1, to calculate 1000 kernel weight and to select families with the best set of alleles and performance. Methods. Polymerase chain reaction for molecular genetic analysis and calculation of the 1,000-kernel weight for wheat grain yields were used. Results. Determination of the three loci Glu-A1, Glu-B1, Glu-D1 shows that among 44 hybrid families it is possible to identify 16 most promising families that have the most valuable allelic variants. Furthemore, given the analysis of the 1,000-kernel weight, two families (#36 and 40) having the highest values can be selected among those 16 families. Conclusions. The results of study enable a comprehensive approach to the selection of progeny with the best genetic base, which will be used in breeding of soft winter wheat later on. Keywords: Triticum aestivum L., gene Gpc-B1, Glu-1 loci, PCR, molecular markers.

Molecular Characterization and Genetic Diversity Study in F3 Population of Rice

2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur
Keyword(s):  
Genetic Distance ◽  
Gene Diversity ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Polymorphic Loci ◽  
Rice Genotypes ◽  
High Level ◽  
Genetic Diversity Study ◽  
Diversity Study

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009

Diagnosis and Management of Cardiomyopathies – A Focus on Genetics, Cardiac Magnetic Resonance and Clinical Features

2011 ◽  
Vol 7 (3) ◽  
pp. 225
Author(s):  
Gianfranco Sinagra ◽  
Michele Moretti ◽  
Giancarlo Vitrella ◽  
Marco Merlo ◽  
Rossana Bussani ◽  
...  
Keyword(s):  
Clinical Practice ◽  
Cardiac Magnetic Resonance ◽  
Magnetic Resonance ◽  
Imaging Techniques ◽  
Molecular Genetic ◽  
Molecular Genetic Analysis ◽  
Routine Clinical Practice ◽  
Clinical Approach ◽  
Diagnosis And Management ◽  
Made In

In recent years, outstanding progress has been made in the diagnosis and treatment of cardiomyopathies. Genetics is emerging as a primary point in the diagnosis and management of these diseases. However, molecular genetic analyses are not yet included in routine clinical practice, mainly because of their elevated costs and execution time. A patient-based and patient-oriented clinical approach, coupled with new imaging techniques such as cardiac magnetic resonance, can be of great help in selecting patients for molecular genetic analysis and is crucial for a better characterisation of these diseases. This article will specifically address clinical, magnetic resonance and genetic aspects of the diagnosis and management of cardiomyopathies.

scholarly journals MOLECULAR GENETIC ANALYSIS OF THE DILUTE-SHORT EAR (d-se) REGION OF THE MOUSE

Genetics ◽  
1986 ◽  
Vol 112 (2) ◽  
pp. 321-342
Author(s):  
Eugene M Rinchik ◽  
Liane B Russell ◽  
Neal G Copeland ◽  
Nancy A Jenkins
Keyword(s):  
Physical Map ◽  
Leukemia Virus ◽  
Molecular Genetic ◽  
Break Point ◽  
Virus Genome ◽  
Molecular Genetic Analysis ◽  
Chromosome 9 ◽  
Genetic Complementation ◽  
Induced Mutations ◽  
Radiation Induced

ABSTRACT Genes of the dilute-short ear (d-se) region of mouse chromosome 9 comprise an array of loci important to the normal development of the animal. Over 200 spontaneous, chemically induced and radiation-induced mutations at these loci have been identified, making it one of the most genetically well-characterized regions of the mouse. Molecular analysis of this region has recently become feasible by the identification of a dilute mutation that was induced by integration of an ecotropic murine leukemia virus genome. Several unique sequence cellular DNA probes flanking this provirus have now been identified and used to investigate the organization of wild-type chromosomes and chromosomes with radiation-induced d-se region mutations. As expected, several of these mutations are associated with deletions, and, in general, the molecular and genetic complementation maps of these mutants are concordant. Furthermore, a deletion break-point fusion fragment has been identified and has been used to orient the physical map of the d-se region with respect to the genetic complementation map. These experiments provide important initial steps for analyzing this developmentally important region at the molecular level, as well as for studying in detail how a diverse group of mutagens acts on the mammalian germline.

scholarly journals Repeated molecular genetic analysis in Brugada syndrome revealed a novel disease-associated large deletion in the SCN5A gene

2016 ◽  
Vol 2 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Anders Krogh Broendberg ◽  
Lisbeth Noerum Pedersen ◽  
Jens Cosedis Nielsen ◽  
Henrik Kjaerulf Jensen
Keyword(s):  
Genetic Analysis ◽  
Brugada Syndrome ◽  
Molecular Genetic ◽  
Large Deletion ◽  
Molecular Genetic Analysis ◽  
Scn5a Gene

scholarly journals Paternal Uniparental Isodisomy of Chromosome 2 in a Patient with CNGA3-Associated Autosomal Recessive Achromatopsia

2021 ◽  
Vol 22 (15) ◽  
pp. 7842
Author(s):  
Susanne Kohl ◽  
Britta Baumann ◽  
Francesca Dassie ◽  
Anja K. Mayer ◽  
Maria Solaki ◽  
...  
Keyword(s):  
Segregation Analysis ◽  
Molecular Genetic ◽  
Retinal Disease ◽  
Underlying Mechanism ◽  
Recurrence Risk ◽  
Molecular Genetic Analysis ◽  
Recessive Mutation ◽  
Chromosome 2 ◽  
Inherited Retinal Disease ◽  
Uniparental Isodisomy

Achromatopsia (ACHM) is a rare autosomal recessively inherited retinal disease characterized by congenital photophobia, nystagmus, low visual acuity, and absence of color vision. ACHM is genetically heterogeneous and can be caused by biallelic mutations in the genes CNGA3, CNGB3, GNAT2, PDE6C, PDE6H, or ATF6. We undertook molecular genetic analysis in a single female patient with a clinical diagnosis of ACHM and identified the homozygous variant c.778G>C;p.(D260H) in the CNGA3 gene. While segregation analysis in the father, as expected, identified the CNGA3 variant in a heterozygous state, it could not be displayed in the mother. Microsatellite marker analysis provided evidence that the homozygosity of the CNGA3 variant is due to partial or complete paternal uniparental isodisomy (UPD) of chromosome 2 in the patient. Apart from the ACHM phenotype, the patient was clinically unsuspicious and healthy. This is one of few examples proving UPD as the underlying mechanism for the clinical manifestation of a recessive mutation in a patient with inherited retinal disease. It also highlights the importance of segregation analysis in both parents of a given patient or especially in cases of homozygous recessive mutations, as UPD has significant implications for genetic counseling with a very low recurrence risk assessment in such families.

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