MODERN OUTLOOKS ON PREVALENCE OF ESR1 AND PRG POLYMORPHISM IN WOMEN OF REPRODUCTIVE AGE WITH ENDOMETRIAL HYPERPLASIA

2020 ◽  
pp. 112-120
Author(s):  
I.M. Ordiyants ◽  
A.A. Kuular ◽  
A.A. Yamurzina ◽  
T.A. Bazieva
Keyword(s):  
Endometrial Hyperplasia ◽  
Progesterone Receptors ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Atypical Endometrial Hyperplasia ◽  
Group 3 ◽  
The Subject ◽  
Polymerase Chain ◽  
Group 2

Prevalence of proliferative processes is very high nowadays. Moreover, such processes easily transform into malignant ones. However, pathogenesis of endometrial hyperplasia (EH) is not fully understood. Imbalance of estrogen and progesterone, as well as estrogen and progesterone receptors is the reason for hyperplastic process onset in hormone-dependent tissues. Currently, there are no markers that could serve as objective predictors for EH development. It is unclear, whether EH transforms into cancer or spontaneously improves. The purpose of the study is to determine prevalence of ESR1 and PRG polymorphism in women of reproductive age with endometrial hyperplasia. Materials and Methods. Trial subjects (n=143) were divided into three groups: Group 1 consisted of 53 patients with glandular and glandular-cystic EH without atypia; Group 2 contained 34 patients with atypical EH; Group 3 was the control group. Polymerase chain reaction of DNA synthesis was used to conduct molecular and genetic loci study. Statistical analysis of the data obtained was performed with SAS JMP 11 and Statistica 10. Results. Mutant CC-allele of PvuII ESR1 polymorphism was found in every fourth woman with glandular endometrial hyperplasia and in every third patient with atypical endometrial hyperplasia. Prevalence of GG genotype of XbaI ESR1 polymorphism did not have any statistically significant differences in comparison with the control group. Mutant TT-allele of Val660Leu PRG polymorphism in glandular endometrial hyperplasia was 1.8 times more common in experimental groups than in the control one. Homozygous AA-genotype of 331G/A PRG polymorphism was not identified in women with endometrial hyperplasia. Conclusion. In endometrial hyperplasia, prevalence of mutant CC-allele of PvuII C/T ESR1 polymorphism leads to a decrease in ERa sensitivity, whereas prevalence of mutant TT-allele of Val660Leu PRG polymorphism leads to impaired sensitivity and a decrease in the biosynthesis rate of progesterone receptors. EH etiology and pathogenesis in women of reproductive age still remains the subject for future scientific research. Keywords: estrogen receptors, progesterone receptors, glandular endometrial hyperplasia, atypical endometrial hyperplasia. Актуальность проблемы пролиферативных процессов связана с высокой частотой распространения и высоким риском их трансформации в злокачественный процесс. В настоящее время до конца не изучен патогенез гиперплазии эндометрия (ГЭ). Причиной формирования гиперпластического процесса в гормонально-зависимых тканях может быть дисбаланс эстрогенов и прогестерона, а также эстрогеновых и прогестероновых рецепторов. В настоящее время отсутствуют маркеры, которые могли бы служить объективными предикторами развития ГЭ в направлении трансформации в рак или, наоборот, спонтанной ее регрессии. Цель исследования. Определить частоту встречаемости полиморфизмов генов ESR1 и PRG у пациенток репродуктивного возраста с различными вариантами гиперплазии эндометрия. Материалы и методы. Все обследованные женщины (143 чел.) были поделены на три группы: I группу составили 53 пациентки с железистой и железисто-кистозной ГЭ без атипии; II группу – 34 пациентки с атипической гиперплазией эндометрия; III группа стала контрольной. Молекулярно-генетическое исследование локусов проведено методом полимеразной цепной реакции синтеза ДНК. Статистический анализ полученных данных выполнен в программах SAS JMP 11 и Statistica 10. Результаты. Мутантный аллель СС полиморфизма PvuII гена ESR1 обнаружен у каждой четвертой женщины с железистой гиперплазией эндометрия и у каждой третей пациентки с атипической гиперплазией эндометрия. Частота встречаемости генотипа GG полиморфизма XbaI гена ESR1 в сравнении с группой контроля не имела статистически значимых различий. Мутантный аллель ТТ полиморфизма Val660Leu гена PRG при железистой гиперплазии эндометрия встречался в 1,8 раза чаще, чем в группе контроля. Гомозиготный генотип АА полиморфизма 331G/A гена PRG у женщин с гиперплазией эндометрия выявлен не был. Выводы. При гиперплазии эндометрия преобладание мутантного аллеля CC полиморфизма PvuII C/T гена ESR1 приводит к снижению чувствительности ERa, а преобладание мутантного аллеля TT полиморфизма Val660Leu гена PRG – к нарушению чувствительности и снижению скорости биосинтеза прогестероновых рецепторов. Предметом исследований по-прежнему остается концепция этиологии и патогенеза ГЭ у женщин репродуктивного возраста, что требует дальнейшего научного поиска. Ключевые слова: эстрогеновые рецепторы, прогестероновые рецепторы, железистая гиперплазия эндометрия, атипическая гиперплазия эндометрия.

Clinical and sonological features in endometriomas in women of reproductive age

2016 ◽  
pp. 85-89
Author(s):  
О. Shapoval ◽  
Keyword(s):  
Endometrial Hyperplasia ◽  
Benign Tumor ◽  
Pain Syndrome ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Ovarian Endometriosis ◽  
Gynecological Examination ◽  
Different Characteristics ◽  
Ovarian Endometriomas

The objective: to study the prevalence of ovarian endometriosis in women of reproductive age, the features of clinical and ultrasound picture of endometriosis. Patients and methods. The study involved 22 patients with endometriomas, the control group – 50 women gynecological and somatically healthy. Results. The incidence of ovarian endometriomas in the structure of benign tumor-like formations of ovaries is 0.62%. In 72.73% of ovarian endometriomas occur on a background of concomitant gynecological pathology. Clinically, in 77.27% of cases there is a pain syndrome, in 59.09% – algomenorrhea, in 13.64% – infertility; 18.18% of cases endometriomas remain «dumb» and proceed with the erased clinical picture. Sonologically in patients with endometriomas adenomyosis, endometrial hyperplasia, changes in the contralateral ovary are determined. Conclusion. Without additional methods of diagnostic gynecological examination may identify the 3 cm tumor-like formation of the ovary with different characteristics, which does not allow to differentiate endometrioma from inflammation, functional and ovarian tumors. Ultrasound can diagnose pathological ovarian formation of 1 cm, detailing the nature of the cyst. Key words: endometriosis, reproductive age, retrospective analysis, ultrasound.

scholarly journals Indicators of the reproductive function after endoscopic surgical treatment of uterine myomas

2020 ◽  
Vol 13 (4) ◽  
pp. 297-304
Author(s):  
M. M. Vysotskiy ◽  
I. I. Kuranov ◽  
O. B. Nevzorov
Keyword(s):  
Reproductive Function ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Hormone Production ◽  
Degree Of Malignancy ◽  
Main Factors ◽  
Group 2 ◽  
Almost All ◽  
Group 1

Aim: to characterize the function of the reproductive system in women after organ-preserving surgery for uterine myoma: hysteroresectoscopic (HRS) myomectomy and laparoscopic (LS) myomectomy.Materials and methods. Forty one patients were examined and divided into 2 groups: Group 1 – 18 patients after HRS myomectomy and Group 2 – 23 patients after LS myomectomy. The control group included 20 healthy women of reproductive age.Results. The HRS operation led to a significant decrease in the production of anti-Mullerian hormone (AMH), estradiol and progesterone, while the levels of luteinizing (LH) and follicle-stimulating (FSH) hormones increased. After myomectomy performed by the laparoscopic access, the levels of estradiol, progesterone, and AMH decreased but the levels of both LH and FSH increased so that the ratio LH/FSH remained unchanged. Almost all indices of gonadotropic and steroid hormone production became normalized over 6 months of the postoperative period.Conclusion. The main factors of unfavorable prognosis in patients with ovarian tecoma are tumor necrosis, degree of malignancy and mitotic activity.

scholarly journals Effekty dlitel'nogo primeneniya estrogen-gestagennoy terapii u zhenshchin reproduktivnogovozrasta s izolirovannym gipogonadotropnymgipogonadizmom

2010 ◽  
Vol 7 (1) ◽  
pp. 52-57
Author(s):  
I A Ilovayskaya ◽  
V Yu Zektser ◽  
A V Il'in ◽  
N P Goncharov ◽  
I I Dedov
Keyword(s):  
Total Cholesterol ◽  
Clinical Symptoms ◽  
Negative Impact ◽  
Mineral Metabolism ◽  
Hypogonadotropic Hypogonadism ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Group 2 ◽  
Group 1

We examined 56 normoprolactinemic women of reproductive age (18-45 y.o.) with isolated hypogonadotropic hypogonadism (group 1) initially and on treatment with 2 mg of 17beta-estradiol and 10 mg of dydrogesterone in sequenced manner (HRT), duration of HRT was from 18 to 42 (median 36) months; 45 healthy women (20-38 y.o.) were included in control group 2. Initially hypercholesterolemia was observed in 50% of cases in group 1 and 6.6% in group 2 (z=12,29, p=0,0005); nevertheless, the difference in lipid levels between groups 1 and 2 was not statistically significant: total cholesterol levels 5,2 (4,3; 6,0) mmol/l and 4,63 (4,15; 5,15) mmol/l respectively (1vs2, р=0,1); triglycerides 0,8 (0,62; 1,3) mmol/l and 0,76 (0,6; 0,85) mmol/l (1vs2, р=0,08); HDL 1,89 (1,24; 2,1) mmol/l and 1,79 (1,44; 2,8) mmol/l (1vs2, p=0,85); LDL 2,7 (2,2; 3,2) mmol/l and 2,75 (2,3; 3,3) mmol/l (1vs2, p=0,64). In group 1 decrease of total cholesterol and triglycerides concentrations was found on HRT: total cholesterol 4,8 (3,95; 5,1) mmol/l (before vs on treatment p=0,041), triglycerides 0,65 (0,6; 0,9) (before vs on treatment p=0,044) respectively, changes in HDL and LDL levels were not revealed. Initial concentrations of Ca++, P, and alkaline phosphatase (AP) were within normal range in all women. However, concentrations of Ca++ and AP were higher in group 1 compared to group 2: Ca++ 1,13 (1,08; 1,19) mmol/l and 1,05 (1,03; 1,09) mmol/l (1vs2, p=0,0016); AP 161,5 (141,8; 183) IU/l and 141,0 (119; 151) IU/l (1vs2, p=0,044). On HRT reduce in Ca++ and AP concentrations was observed: Ca++ concentrations 1,05 (1,03; 1,10) mmol/l (before vs on treatment p=0,004), AP 139 (112; 143) IU/l (before vs on treatment p=0,004). HRT was accompanied by improvement of clinical symptoms, had no influence on thyroid function, and provoked physiological rise of prolactin levels.Thus, despite lacking the expressed biochemical disorders, isolated hypogonadotropic hypogonadism in women of reproductive age have hidden negative impact on lipid and mineral metabolism. Long term HRT is safe and significantly improved parameters of lipid and mineral homeostasis in this cohort of patients.

scholarly journals The Effect of Anti Masquito Electric Gas that Consist Allethrin That Influence The Weight and Colour of Rats Liver

2017 ◽  
Vol 6 (2) ◽  
pp. 6
Author(s):  
Nurmawanti Nurmawanti ◽  
Ayly Soekanto
Keyword(s):  
Male Rats ◽  
Control Group ◽  
Control Groups ◽  
Control Group Design ◽  
Significant Difference ◽  
Post Test ◽  
Group 3 ◽  
The Subject ◽  
Group 2 ◽  
Group 1

The aim of this research is to know the influence of anti mosquito electric gas that consisted Allethrin to the weight and colour of the rats liver. This research used an experimental method with the post test only  control group design.  The subject of this research is male rats weight of 150 grams each and total there was 24 rats that were divided to 4 groups. The first one, the  control groups (PO) was not given any of the gas, the second one, group 1 (P1) was given the gas for 4 hours everday, the third one, group  2 (P2), was given for 6 hours and last, group 3  (P3) was given 8 hours everyday. After later, on  30 Th day, the rats were terminated and being put in a surgery to remove their liver. This data was analyzed using SPPS for windows version 16. To see the differences in weight between the groups, it was analyzed using anova, and  to obtain the discoloration of the rats liver was analyzed using Kruskal Wallis Test. From the statistic tests, it show that there is significant difference in weight and color of liver in the group that α ≤ 0,05. According Anova Test, it shows that there is a significant difference α = 0,034 and from Kruskal Wallis test α = 0,013. In the conclusion anti mosquito electric gas that consist Allethrin affects the weight and the color of rats liver.

scholarly journals The frequency and structure of metabolic disorders in obese women in combination with polycystic ovary syndrome

2021 ◽  
Vol 18 (3) ◽  
pp. 254-262
Author(s):  
E. V. Bolotova ◽  
A. V. Dudnikova ◽  
V. A. Krutova ◽  
N. S. Prosolupova
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Metabolic Disorders ◽  
Polycystic Ovary ◽  
Reproductive Age ◽  
Control Group ◽  
Obese Women ◽  
Women Of Reproductive Age ◽  
Ovary Syndrome ◽  
Group 2 ◽  
Group 1

Background: Obesity is considered a global epidemic and is one of the most significant medical and social problems. Research in recent years shows that in 25-45% of cases of obesity, polycystic ovary syndrome (PCOS) is detected. The influence of obesity on the pathogenesis of metabolic disorders in this category of patients remains controversialAims: to determine the frequency and structure of metabolic disorders in obese women in combination with polycystic ovary syndrome (PCOS).Materials and methods: A single-center cross-sectional sample survey of women of reproductive age was conducted. The study included clinical anthropometry with measurement of body weight, height, waist circumference (OT) and hip circumference (OB), followed by calculation of body mass index (BMI) and OT/OB ratio, and measurement of blood pressure (BP). A biochemical blood test was performed, the hormonal status was examined, and an ultrasound examination of the pelvic organs was performed.Results: A survey of 136 women of reproductive age was conducted. Group 1 included obese women without PCOS (59 patients), group 2 included obese women in combination with PCOS (45 patients), and the control group included 38 healthy women. Group 1 included obese women without PCOS (59 patients), and group 2 included obese women with PCOS (45 patients), in the control group — 38 healthy women. Among group 2 patients, dyslipidemia, visceral obesity, arterial hypertension, insulin resistance, hyperinsulinemia, and hyperuricemia were significantly more common (p<0,05). Correlations between BMI and triglycerides, testosterone and total cholesterol were found (p<0,05). Obese and PCOS patients had significantly higher levels of C-reactive protein(CRP), fibrinogen, anti-Müllerian hormone (AMH), and hepatic transaminases (p<0,05). Vitamin D deficiency was detected in 13,2% of patients, and insufficiency — in 22,7% of patients. There is a statistically significant correlation between the level of 25 (OH)D and indicators of BMI, follicle-stimulating hormone, luteinizing hormone (p<0,05), the level of AMH (p=0,008).Conclusions: A high frequency of metabolic disorders in obese women in combination with PCOS has been identified, which necessitates early screening, diagnosis and treatment of these disorders to strengthen reproductive health and prevent chronic non-communicable diseases.

scholarly journals Comparative aspects of treatment of endometrial hyperplasia in women of reproductive age with overweight

2021 ◽  
pp. 3-9
Author(s):  
Andrii Semeniuk
Keyword(s):  
Gnrh Agonist ◽  
Endometrial Hyperplasia ◽  
Ethinyl Estradiol ◽  
Endometrial Thickness ◽  
Uterine Bleeding ◽  
Reproductive Age ◽  
Women Of Reproductive Age ◽  
Morphological Examination ◽  
Group 2 ◽  
Group 1

The aim. Minimization of the frequency of recurrence of endometrial hyperplasia (EH) in women of reproductive age with overweight (OW) depending on the tactics of treatment. Materials and methods. 90 women of reproductive age with endometrial hyperplasia and OW were selected. They, in turn, were divided into three groups: group 1 – 30 women who took a gonadotropin-releasing hormone agonist (GnRH agonist), namely at a dose of 3.75 mg intramuscularly once every 28 days; group 2 – 30 women who used progestin (norethisterone) at a dose of 10 mg per day from day 16 to 25 of the cycle, group 3 – 30 women who took combined oral contraception (COC) (30 mcg ethinyl estradiol and 150 mcg desogestrel) in a cyclic mode 21/7. Evaluation of the effectiveness of therapy included a clinical picture of the disease 6 months after the start of treatment, assessment of the variability of the average values of endometrial thickness and uterine size on ultrasound (US) of the pelvis 6 months after treatment. Also analyzed the effectiveness of therapy based on the results of morphological examination of the endometrium in a biopsy of the uterine mucosa, performed 6 months after the start of treatment. In addition, a general analysis of the frequency of EH recurrence was performed 6–24 months after treatment. Results. The results showed that in the group in which women were prescribed GnRH agonist, there was a significantly higher effectiveness of treatment, in particular the absence of uterine bleeding and menstrual disorders (MD). At the same time, in the other norethisterone group, 53.3 % (16) of women had intermenstrual uterine bleeding. In patients in the group in which women received COC, uterine bleeding was observed in 30.0 % (i.e. 9) of patients (p <0.05). Immediately after treatment, the average values of endometrial thickness in patients of group 1, according to ultrasound, was 3.59±0.47 mm, which was significantly less than in women of groups 2 and 3 – 6.81±0.59 mm (p<0.001) and 7.58±0.69 mm (p<0.001). In addition, patients in group 1 at 3, 6, 12, 24 months after the end of hormone therapy were registered significantly lower average values of endometrial thickness, compared with patients receiving norethisterone and estrogen-progestogen drugs. Conclusions. In a comparative evaluation of the effectiveness of treatment of endometrial hyperplastic processes in overweight women, it was found that the recurrence rate after 6–24 months occurs in 6.7 % (i.e. 2) of patients after GnRH agonist therapy, in 33.3 % (i.e. in 10) patients receiving norethisterone (p<0.001), and in 50 % (i.e. 15) of women treated with COC (p<0.001).

scholarly journals Clinical characteristics of women with endometrial hyperplasia

2021 ◽  
Vol 4 (2) ◽  
pp. 124-129
Author(s):  
K.K. Salamova ◽  
◽  
L.V. Saprykina ◽  
A.M. Ramazanova ◽  
Z.T. Mil’dzikhova ◽  
...  
Keyword(s):  
Clinical Characteristics ◽  
Endometrial Hyperplasia ◽  
Uterine Fibroids ◽  
Control Group ◽  
Endocrine Disorders ◽  
Endometrial Polyps ◽  
Somatic Status ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Aim: to describe clinical characteristics of women with endometrial hyperplasia. Patients and Methods: this retrospective single-center study enrolled 86 women who underwent hysteroscopic curettage (with subsequent histology) on the basis of complaints and ultrasound. All women were divided into three groups. Group 1 included 30 women with endometrial hyperplasia (EH). Group 2 included 30 women with endometrial polyps (EP). Group 3 included 26 healthy women without any endometrium conditions (control group). Somatic status, obstetric gynecologic anamnesis, and comorbidities were evaluated. Results: in women with EH, heavy periods were 2.37- and 2.6-times more common than in groups 2 and 3 (p<0.05) while the rate of other abnormal uterine bleedings was 7.06- and 6.06-times higher, respectively (p<0.05). In women with EH, anemia was diagnosed 4- and 5.2-times more common than in groups 2 (p<0.05) and 3 (p<0.05), respectively. In group 2, inflammatory cervical and vaginal conditions were 3.98- and 3.46-times more common than in group 1 (p<0.05) and group 3 (p>0.05). The occurrence of endocrine disorders in group 1 was 4.49- and 3.9-times higher than in group 2 and 3, respectively (p<0.05). Anamnestic thrombosis, strokes, and migraines were reported only in women with EH. Conclusions: prevention of endometrium conditions entails both early detection and control over ovulation and management of endocrine disorders and treatment of genital tract inflammation. When managing women with uterine fibroids and adenomyosis and anamnestic thrombosis, a doctor should be vigilant about EH. KEYWORDS: endometrial hyperplasia, endometrial polyp, inflammation, hyperplastic conditions, uterus, thrombosis. FOR CITATION: Salamova K.K., Saprykina L.V., Ramazanova A.M. et al. Clinical characteristics of women with endometrial hyperplasia. Russian Journal of Woman and Child Health. 2021;4(2):124–129. DOI: 10.32364/2618-8430-2021-4-2-124-129.

scholarly journals The application of ultrasonography to select tactics for treatment of women of reproductive age with uterine leiomyoma

2020 ◽  
Vol 24 (4 (96)) ◽  
pp. 32-40
Author(s):  
A. Kornatska ◽  
A. Dubchak ◽  
M. Flaksemberg ◽  
I. Baranetska
Keyword(s):  
Conservative Treatment ◽  
Uterine Leiomyoma ◽  
Ultrasound Examination ◽  
Reproductive Age ◽  
Control Group ◽  
Women Of Reproductive Age ◽  
Pelvic Organs ◽  
Group 2 ◽  
Doppler Color ◽  
Group 1

Objective. To study the condition of the pelvic organs in women of reproductive age with uterine leiomyoma UL, depending on the type of treatment based on ultrasonographic monitoring.Materials and methods. 299 women of reproductive age were examined. Patients were divided into 3 groups: 1 group consisted of 159 women with uterine leiomyoma (UL) who underwent surgical treatment, 2 group – 120 women with UL who underwent conservative treatment, 3 control group consisted of 20 healthy women of reproductive age. In order to obtain the most complete information about the size of the uterus, the structure and topography of myomas, the features of their vascularization, patients underwent ultrasound before and 3 months after treatment.Results. Ultrasound examination of the average parameters of the uterine body in the main groups showed an excess of these indicators relative to the control group (3.2 times in group 1 and 1.3 times in group 2). Uterine volume was greater in women of group 1 compared with women of groups 2 and 3 (p <0.05). In most cases, in 139 (49.0%) women of the main groups, the localization of fibroids was intramural with central growth, without changing the configuration of the uterus from the outside and was not deformed cavity. Whereas, intramural nodes with centripetal growth deformed the uterine cavity in 39 (14.0%) cases, and centrifugal - in 100 (36.0%) subjects - its contour. Mixed and central types of angioarchitectonics were characteristic of women with the largest uterine sizes. In dynamic ultrasound examination in women after conservative myomectomy who did not receive hormone therapy, the level of myometrial perfusion was higher than in those who received gonadotropin-releasing hormones before and after treatment, which may be more prognostically favorable for UL recurrence. Women in group 2 after receiving conservative treatment had a positive tendency to reduce the volume of the uterus and fibroids.Conclusions. Ultrasound diagnostics can be considered a highly informative, non-invasive method that helps to identify the presence, location, structure, size of the myoma, the presence of concomitant pathology of the pelvic organs, as well as in addition to Doppler color flow mapping and pulsed Doppler blood allows us to detect the presence of tumor blood circulation. Echographic and Doppler evaluation of uterine leiomyoma with peculiarities of vascularization in the diagnostic algorithm helps to differentiate the choice of the method of treatment (conservative or surgical).

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