scholarly journals Optimization of the microwave assisted extraction and biological activities of polyphenols from lemon verbena leaves

2021 ◽  
Vol 45 (1) ◽  
pp. 157-177
Author(s):  
Zohra Touati ◽  
Menana Guemghar ◽  
Kenza Bedjaoui ◽  
Nabila Emilia Djerrada ◽  
Kahina Djaoud ◽  
...  
Keyword(s):  
Irradiation Time ◽  
Biological Activities ◽  
Reducing Power ◽  
Biological Properties ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Disk Diffusion Assay ◽  
Lemon Verbena

The present study aims to optimize the extraction of phenolics by microwave-assisted extraction (MAE) using the response surface methodology (RSM), from Lemon verbena leaves. The optimized extract was tested for its antioxidant activity using two methods (DPPH and reducing power) and its antibacterial efficiency by using disk diffusion assay and broth microdilution, against two Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and two Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633) strains. Under the optimized conditions (40% (v/v): of ethanol concentration, 188 s of irradiation time, 600 W of microwave power and 1:40 g/mL of solid-to-liquid ratio) the total phenolic content (TPC) was 67.87±1.61 mg GAE/g DW. The IC50 of the extract was 139.65±1.44 µg/mL and 56.60±2.79 µg/mL for DPPH inhibition and reducing power, respectively. The best antibacterial activity was shown by the extract obtained by MAE with lower MBC (1.56 to 18.75 mg/mL) and MBC/MIC ratio. Lemon verbena extract can be used as an ingredient in cosmetics, food supplements and herbal medicinal products due to its interesting biological properties.

scholarly journals RECOVERY OF THE BIOLOGICAL ACTIVE COMPOUNDS OF Musa Sp. THROUGH MICROWAVE ASSISTED EXTRACTION

2017 ◽  
Vol 18 (2) ◽  
pp. 105-116 ◽  
Author(s):  
Farhan M. Said
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compound ◽  
Phenolic Content ◽  
Irradiation Time ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Liquid Ratio ◽  
Microwave Assisted ◽  
Musa Sp ◽  
Assisted Extraction

The objective of this research was to identify the recovery of the biological active compound of Musa sp. thru microwave assisted extraction (MAE) system. Antioxidant activity and phenolic compound were investigated on the effect of temperature, microwave power, irradiation time and solid to liquid ratio. The extraction process was carried out using only distilled water as an extracting agent. The result showed that, at 70oC fresh Musa sp. extracted 45% higher antioxidant compared to the dried Musa sp. 500 W was the best power of MAE to extract antioxidant and phenolic compound of ripe Musa sp., while for unripe Musa sp. 800 W was the best. The best irradiation time was 90 s, where it extracted almost 90% antioxidant activity of ripe and unripe Musa sp. Almost 480 mg GA/100 g FW of total phenolic content was extracted on the unripe peel. Solid to liquid ratio of 3 : 60 was the best parameter to extract antioxidant and phenolic content of ripe and unripe Musa sp. Overall, antioxidant and phenolic compound were significantly higher in the unripe peel, followed by ripe peel, unripe pulp and ripe pulp.

scholarly journals Optimization of Microwave-Assisted Extraction of Total Phenolic and Total Flavonoid Contents from Fruits of Docynia indica (Wall.) Decne. Using Response Surface Methodology

Processes ◽  
2019 ◽  
Vol 7 (8) ◽  
pp. 485 ◽  
Author(s):  
Xuan Le ◽  
Manh Nguyen ◽  
Dinh Vu ◽  
Minh Pham ◽  
Quoc Pham ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Biological Activities ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Phenolic And Flavonoid

Docynia indica (D. indica) shows various useful biological activities, such as antioxidant, anti-inflammatory, antibacterial effects, and positive benefits for human health. Such biological activities relate to the main phytochemicals of D. indica including phenolic and flavonoid. However, isolation for phenolic and flavonoid by popular methods such as hot extraction, soxhlet extraction, and ultrasonic extraction have been relatively ineffective. Therefore, in this study, microwave-assisted extraction (MAE) was used for the extraction of total phenolic and total flavonoid from D. indica. The optimization experiments were conducted based on response surface methodology (RMS) according to a central composite design with four independent variables: extraction time (min), ethanol concentration (%, v/v), microwave power (W), and pH of the solvent. Three dependent variables were total phenolic content (TPC), total flavonoid content (TFC), and yield. The optimal conditions for the extraction of phenolic and flavonoid from D. indica were: extraction time of 50 min, ethanol concentration of 65%, microwave power of 440 W, and solvent pH of 5.4. These conditions corresponded to TPC, TFC, and yield values of 33.57 ± 0.12 (mg GAE/g), 25.01 ± 0.11 (mg QE/g) and 33.44 ± 0.14 (%), respectively.

Optimization of Bioactive Compounds with Antioxidant Activity of Himanthalia elongata by Microwave-Assisted Extraction Using Response Surface Methodology

2021 ◽  
Vol 5 (1) ◽  
pp. 70
Author(s):  
Franklin Chamorro ◽  
Lucia Cassani ◽  
Catarina Lourenço-Lopes ◽  
Anxo Carreira-Casais ◽  
Maria Carpena ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Himanthalia Elongata ◽  
Biological Tests ◽  
Ethanol In Water

Himanthalia elongata is a brown alga used in applications in the food, pharmaceutical and nutraceutical industries due to its biological properties, such as antioxidant, anti-inflammatory, and antimicrobial, among others. These effects are attributed to the high content of nutrients and secondary metabolites, especially phenolic compounds. The objective of this study is to optimize the microwave-assisted extraction method to recover phenolic compounds and flavonoids, considering three extraction parameters: the concentration of ethanol in water, the extraction time and pressure. The total phenolic content and the total flavonoid content were evaluated, and two biological tests were performed to assess the antioxidant properties.

scholarly journals Microwave-Assisted Extraction of Phenolic Compounds from Melastoma sanguineum Fruit: Optimization and Identification

Molecules ◽  
2018 ◽  
Vol 23 (10) ◽  
pp. 2498 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Jiao-Jiao Zhang ◽  
Ya Li ◽  
Xiao Meng ◽  
Hua-Bin Li
Keyword(s):  
Phenolic Compounds ◽  
Irradiation Time ◽  
Soxhlet Extraction ◽  
Dry Weight ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Epicatechin Gallate ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Temperature Time

A microwave-assisted extraction (MAE) technology optimized by response surface methodology (RSM) was established to extract phenolic compounds from the fruit of Melastoma sanguineum. The effects of solvent composition, ratio of solvent to material, temperature, time and microwave power on phenol yield were evaluated in single-factor tests. The three parameters exerting main impacts on phenol yield were further optimized by RSM. Under optimal extraction conditions (31.33% ethanol, solvent/material ratio of 32.21 mL/g, 52.24 °C, 45 min and 500 W), the total phenolic content was 39.02 ± 0.73 mg gallic acid equivalent (GAE)/g dry weight (DW). This MAE method performed better in comparison with two conventional methods, those being maceration (25.79 ± 1.03 mg GAE/g DW) and Soxhlet extraction (18.40 ± 1.34 mg GAE/g DW), using lower process temperature, shorter irradiation time, and lower organic solvent consumption. In addition, five flavonoids (epicatechin gallate, epicatechin, rutin, pigallocatechin and quercetin) and two phenolic acids (protocatechuic acid and chlorogenic acid) in the extract were identified and quantified using UPLC-MS/MS.

scholarly journals TWO LEVEL FACTORIAL SCREENING OF MICROWAVE-ASSISTED EXTRACTION PARAMETERS FOR THE RECOVERY OF PHENOLIC COMPOUNDS FROM VERNONIA CINEREA LEAF

2019 ◽  
Vol 5 (1) ◽  
pp. 16-28
Author(s):  
Oluwaseun Ruth Alara ◽  
Nour Hamid Abdurahman ◽  
John Adewole Alara
Keyword(s):  
Phenolic Compounds ◽  
Microwave Power ◽  
Ethanol Concentration ◽  
Irradiation Time ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Vernonia Cinerea ◽  
Phenolics Compounds

Vernonia cinerea is one of the medicinal plants with several potentials for treating different ailments. In the present study, Microwave-assisted extraction (MAE) was employed in extracting phenolics compounds from this plant. However, different factors that affect this extraction method in the recovery of phenolics compounds abound, these factors need to be screened to determine actual contributing factor in order to minimize cost. Irradiation time (1-5 min), ethanol concentration (20-60% v/v), microwave power (40-80 W), extraction temperature (40-80 oC), and feed/solvent (1:10 - 1:18 g/mL) have been screened using two-factorial design for the recoveries of phenolic compounds from V. cinerea leaves. The results obtained in this study indicated that only microwave power, ethanol concentration, irradiation time and feed/solvent contributed to recoveries of total phenolic content (TPC) and total flavonoid content (TFC) from V. cinerea leaves. Thus, these factors at these ranges can be further optimized to obtain optimal yields of phenolic compounds from V. cinerea leaves.

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

Microwave-Assisted Extraction of Chlorophyll from Filter Mud of Sugercane Mill and Component Analysis

2012 ◽  
Vol 518-523 ◽  
pp. 430-435 ◽  
Author(s):  
Hai Rong Guo ◽  
Shao Ying Ma ◽  
Xiao Fei Wang ◽  
Er Fang Ren ◽  
Yuan Yuan Li
Keyword(s):  
Irradiation Time ◽  
Extraction Time ◽  
Extraction Temperature ◽  
Microwave Assisted Extraction ◽  
Optimal Conditions ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Conventional Extraction ◽  
Filter Mud ◽  
Strong Absorption Peak

Microwave-assisted extraction (MAE) was used to extract chlorophylls from filter mud. Ethanol was used as the solvent. The optimal conditions for the MAE of chlorophylls were concluded from the study as the irradiation time, 50 s, the ratio of liquid to solid, 8:1 (mL/g), the extraction temperature, 40 °C, and the extraction time, 60 min. Compared with conventional extraction, the MAE of chlorophylls from the filter mud was more effective. The extraction time for MAE was 60 min with 0.277 mg/g chlorophyll yield, while conventional extraction needed 240 min with only about 0.259 mg/g chlorophyll yield. The Ultraviolet Absorption Spectra of the extracted chlorophylls showed that there was a strong absorption peak at about 663 nm. C=N, Mg-N and C-N was not seen existed from the infrared spectroscopy probably because that the mixed extracts were not purified and the chlorophyll content was less.

scholarly journals Microwave-assisted extraction of non-polar compounds from pistachio hull and characterization of extracts

2018 ◽  
Vol 69 (3) ◽  
pp. 260 ◽  
Author(s):  
H. N. Özbek ◽  
D. Koçak Yanık ◽  
S. Fadıloğlu ◽  
H. Keskin Çavdar ◽  
F. Göğüş
Keyword(s):  
Microwave Power ◽  
Polar Compounds ◽  
Good Alternative ◽  
Tocopherol Content ◽  
Extraction Time ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Solid Ratio ◽  
Microwave Assisted ◽  
Assisted Extraction

Soxhlet and microwave assisted extraction (MAE) methods were used to obtain non-polar compounds from pistachio hull. MAE parameters (liquid to solid ratio, microwave power, and extraction time) were studied to obtain maximum extraction yield. The optimal conditions were found to be liquid to solid ratio of 15:1 (v/w), microwave power of 250 W and extraction time of 12.5 min. The extraction yields were 9.81 and 9.50% for MAE and Soxhlet methods, respectively. The total phenolic content, antioxidant activity and tocopherol content of the extract obtained by MAE was found to be significantly higher than those of the Soxhlet extract (p < 0.05). The results showed that the extract contained α-tocopherols (567.65 mg/kg) and oleic acid (48.46%) as the major tocopherols and fatty acids. These findings propose that hull extracts can be considered as a good source of natural bioactive compounds and MAE can be a good alternative to the traditional Soxhlet method.

scholarly journals Evaluation of Extracts Obtained from FruitWastes Using Different Methods

Ingeniería ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 77-92
Author(s):  
Ciliana Florez Montes ◽  
Andrés Felipe Rojas González ◽  
Sneyder Rodríguez Barona
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Grape Seeds ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Pharmaceutical Industries

Context: Currently, the increase in agroindustrial waste generation has encouraged the search for viable use alternatives. In this paper, four methods to obtain extracts from mango, soursop, and grape peels, as well as and grape seeds, are studied. Their efficiency is analyzed through extraction yields and antioxidant capacity characterization of the extracts. Method: The extraction was performed using solvent, Soxhlet, microwave-assisted, and ultrasound assisted extraction. The characterization of the extracts was made by total phenolic compounds and flavonoids quantification, as well as antioxidant capacity determination, using the DPPH, FRAP, and ORAC tests. Results: It was found that grape seed extracts obtained by different extraction methods, highlighting those obtained by microwave assisted extraction, present a high total content phenolic compounds (>321.381,41 ± 3.476,85 μg Gallic Acid/g) and flavonoids (>103.232,01 ± 4.638,19 μg Quercetin/g), in addition to high antioxidant activity, according to the results of the DPPH (<1,06 ± 0,01), FRAP (>152.280,08 ± 5.197,53 µg TROLOX/g), and ORAC (>124.566,81 ± 581,96 μg TROLOX/g) tests. Conclusions: The results presented in this study suggest that the extracts obtained from grape seeds, especially those obtained by means of microwave-assisted extraction, have a potential use in food and pharmaceutical industries, due to their high antioxidant capacity and their total phenolic compounds and flavonoids content.

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