Optimization of Bioactive Compounds with Antioxidant Activity of Himanthalia elongata by Microwave-Assisted Extraction Using Response Surface Methodology

2021 ◽  
Vol 5 (1) ◽  
pp. 70
Author(s):  
Franklin Chamorro ◽  
Lucia Cassani ◽  
Catarina Lourenço-Lopes ◽  
Anxo Carreira-Casais ◽  
Maria Carpena ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Properties ◽  
Biological Properties ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Himanthalia Elongata ◽  
Biological Tests ◽  
Ethanol In Water

Himanthalia elongata is a brown alga used in applications in the food, pharmaceutical and nutraceutical industries due to its biological properties, such as antioxidant, anti-inflammatory, and antimicrobial, among others. These effects are attributed to the high content of nutrients and secondary metabolites, especially phenolic compounds. The objective of this study is to optimize the microwave-assisted extraction method to recover phenolic compounds and flavonoids, considering three extraction parameters: the concentration of ethanol in water, the extraction time and pressure. The total phenolic content and the total flavonoid content were evaluated, and two biological tests were performed to assess the antioxidant properties.

scholarly journals Microwave-Assisted Extraction and Ultrasound-Assisted Extraction of Bioactive Compounds from Grape Pomace

2020 ◽  
Vol 16 (1-2) ◽  
Author(s):  
Cassiano Brown da Rocha ◽  
Caciano Pelayo Zapata Noreña
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
Grape Pomace ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ultrasound Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Ultrasound Assisted

AbstractThe grape pomace is a by-product from the industrial processing of grape juice, which can be used as a source of bioactive compounds. The aim of this study was to separate the phenolic compounds from grape pomace using an acidic aqueous solution with 2 % citric acid as a solvent, using both ultrasound-assisted extraction, with powers of 250, 350 and 450 W and times of 5, 10 and 15 min, and microwave-assisted extraction using powers of 600, 800 and 1,000 W and times of 5, 7 and 10 min. The results showed that for both methods of extraction, the contents of total phenolic compounds and antioxidant activity by ABTS and DPPH increased with time, and microwave at 1,000 W for 10 min corresponded to the best extraction condition. However, the contents of phenolic compounds and antioxidant activity were lower than exhaustive extraction using acidified methanol solution.

scholarly journals Evaluation of Extracts Obtained from FruitWastes Using Different Methods

Ingeniería ◽  
2020 ◽  
Vol 26 (1) ◽  
pp. 77-92
Author(s):  
Ciliana Florez Montes ◽  
Andrés Felipe Rojas González ◽  
Sneyder Rodríguez Barona
Keyword(s):  
Phenolic Compounds ◽  
Antioxidant Capacity ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Grape Seeds ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Pharmaceutical Industries

Context: Currently, the increase in agroindustrial waste generation has encouraged the search for viable use alternatives. In this paper, four methods to obtain extracts from mango, soursop, and grape peels, as well as and grape seeds, are studied. Their efficiency is analyzed through extraction yields and antioxidant capacity characterization of the extracts. Method: The extraction was performed using solvent, Soxhlet, microwave-assisted, and ultrasound assisted extraction. The characterization of the extracts was made by total phenolic compounds and flavonoids quantification, as well as antioxidant capacity determination, using the DPPH, FRAP, and ORAC tests. Results: It was found that grape seed extracts obtained by different extraction methods, highlighting those obtained by microwave assisted extraction, present a high total content phenolic compounds (>321.381,41 ± 3.476,85 μg Gallic Acid/g) and flavonoids (>103.232,01 ± 4.638,19 μg Quercetin/g), in addition to high antioxidant activity, according to the results of the DPPH (<1,06 ± 0,01), FRAP (>152.280,08 ± 5.197,53 µg TROLOX/g), and ORAC (>124.566,81 ± 581,96 μg TROLOX/g) tests. Conclusions: The results presented in this study suggest that the extracts obtained from grape seeds, especially those obtained by means of microwave-assisted extraction, have a potential use in food and pharmaceutical industries, due to their high antioxidant capacity and their total phenolic compounds and flavonoids content.

scholarly journals Optimization of Microwave-Assisted Extraction of Antioxidants from Bamboo Shoots of Phyllostachys pubescens

Molecules ◽  
2020 ◽  
Vol 25 (1) ◽  
pp. 215 ◽  
Author(s):  
Gualtiero Milani ◽  
Francesca Curci ◽  
Maria Maddalena Cavalluzzi ◽  
Pasquale Crupi ◽  
Isabella Pisano ◽  
...  
Keyword(s):  
Antioxidant Properties ◽  
Extraction Process ◽  
Biologically Active ◽  
Total Phenolic ◽  
Phyllostachys Pubescens ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Bamboo Shoots ◽  
Assisted Extraction ◽  
Time And Energy

Bamboo is a well-known medicinal plant in Southeast Asia that recently has attracted attention for its high polyphenol content and its medical and nutraceutical applications. In this work, polyphenols have been recovered for the first time by microwave-assisted extraction (MAE) from an unusual Italian cultivar of Phyllostachys pubescens bamboo shoots. The effects of three independent variables, such as extraction time, temperature, and solid/liquid ratio, on polyphenol recovery yield were investigated and successfully optimized through the response surface methodology. We demonstrated that MAE is an excellent polyphenols extraction technique from bamboo shoots because the total phenolic content obtained under microwave irradiation optimal conditions (4 min at 105 °C with 6.25 mg/mL ratio) was about eight-fold higher than that obtained with the conventional extraction method. Furthermore, higher total flavonoid content was also obtained under MAE. Consistent with these results, MAE enhanced the extract antioxidant properties with significant improved DPPH, ABTS, and FRAP scavenging ability. Therefore, this innovative extraction process enhances the recovery of biologically active compounds from Phyllostachys pubescens bamboo shoots with a dramatic reduction of time and energy consumption, which paves the way for its industrial application in functional food production.

scholarly journals PHYTOCHEMICAL AND PHARMACOLOGICAL ASPECTS OF ANTHRAQUINONES OF RHEUM AUSTRALE D. DON

2020 ◽  
Vol 6 (2) ◽  
pp. 114-119
Author(s):  
Dwi Hartanti ◽  
Arinda Nur Cahyani
Keyword(s):  
Phenolic Compounds ◽  
Extraction Method ◽  
Literature Search ◽  
Antioxidant Properties ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Chromatographic Techniques ◽  
Assisted Extraction ◽  
Pure Compounds ◽  
Rheum Australe

Anthraquinones are phenolic compounds known best for their laxative activity. Rheum australe D. Don (Indian rhubarb, Polygonaceae) is a prominent medicinal plant with anthraquinones as the bioactive compounds, among a few others. The online literature search was carried out to collect data on the phytochemistry and pharmacological activity of anthraquinones, particularly those isolated from R. australe. Anthraquinones are commonly found in the genera of Rheum, Senna, Aloe, Frangula, and Rubia, which can be quantitatively and qualitatively determined using both conventional and advanced analytical methods. The anthraquinones of R. australe were found in free and glycosidic forms, which were best extracted by the microwave-assisted extraction method. Various chromatographic techniques were commonly conducted to isolate the pure compounds. In addition to its laxative activity, anthraquinones of R. australe also showed potential antibacterial, cytotoxic, and antioxidant properties.

Microwave-Assisted Extraction of Phenolic Compounds from Dried Waste Grape Skins

2015 ◽  
Vol 11 (3) ◽  
pp. 359-370 ◽  
Author(s):  
Miguel A. Pedroza ◽  
Danila Amendola ◽  
Luana Maggi ◽  
Amaya Zalacain ◽  
Dante M. De Faveri ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Large Scale ◽  
Extraction Time ◽  
Process Efficiency ◽  
Total Phenolic ◽  
Energetic Efficiency ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Grape Skins

Abstract Microwave-assisted extraction (MAE) was investigated for recovering of total phenolic compounds from dried waste grape skins using a domestic microwave oven. Influence of vessel geometry, irradiation cycles, irradiation power and time was investigated. The results in terms of phenolics yield, antioxidant capacity and energy consumption were compared with a reference solid–liquid extraction (SLE) carried out for 2 h at 60°C. Equivalent yield of total phenolics as in SLE was achieved with a MAE extraction time of 1,033 sec (corresponding to 83 sec of irradiation at 900 W, 83% saving in extraction time compared to SLE and with a 70% energetic efficiency). Pre-maceration of samples and solvent pre-heating are proposed for large-scale industrial processes to enhance phenolics extraction and process efficiency.

scholarly journals Development of edible Thai rice film fortified with ginger extract using microwave-assisted extraction for oral antimicrobial properties

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Niramon Utama-ang ◽  
Sirinapa Sida ◽  
Phenphichar Wanachantararak ◽  
Arthitaya Kawee-ai
Keyword(s):  
Phenolic Compounds ◽  
Antimicrobial Properties ◽  
Edible Film ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Ginger Extract ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Full Factorial ◽  
Gallic Acid Equivalent

AbstractThis study aimed to investigate microwave-assisted extraction (MAE) of dried ginger and to develop a rice-based edible film incorporating ginger extract. The optimal MAE conditions of 400 W microwave power and an extraction time of 1 min were determined using a 32 full factorial design. The optimized extract showed total phenolic compounds (TPC, 198.2 ± 0.7 mg gallic acid equivalent/g), antioxidant activity measured by DPPH (91.4 ± 0.6% inhibition), ABTS (106.4 ± 3.1 mg Trolox/g), and FRAP (304.6 ± 5.5 mg Trolox/g), and bioactive compounds including 6-gingerol (71.5 ± 3.6 mg/g), 6-shogaol (12.5 ± 1.0 mg/g), paradol (23.1 ± 1.1 mg/g), and zingerone (5.0 ± 0.3 mg/g). Crude extract of dried ginger showed antimicrobial activity against Streptococcus mutans DMST 18777, with a minimum inhibitory concentration and minimum bactericidal concentration of 0.5 and 31.2 mg/mL, respectively. The rice-based edible film incorporating 3.2% (w/v) ginger extract tested against S. mutans DMST 18777 had a mean zone of inhibition of 12.7 ± 0.1 mm. Four main phenolic compounds, 6-gingerol, 6-shogaol, paradol, and zingerone, and six volatile compounds, α-curcumene, α-zingiberene, γ-muurolene, α-farnesene, β-bisabolene, and β-sesquiphellandrene, were found in rice film fortified with crude ginger extract.

scholarly journals Microwave Assisted Extraction of Papaya Leaves and Investigation on Antioxidant Activity

2021 ◽  
pp. 115-122
Author(s):  
. Shobitharai ◽  
Divya Jyothi ◽  
Swathi Das ◽  
C. M. Sumayya ◽  
A. Thabsheer
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Extraction Efficiency ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Conventional Extraction

Microwave assisted extraction (MAE) has gained lot of attention due to its advantages such as less solvent consumption, short time period, higher extraction efficiency, therefore serves as better alternative for conventional extraction methods of plant materials. Plant phenolic compounds are important constituents responsible for reducing the oxidative stress that induces tissue damage which is the one of the major causative factors associated with the chronic disease. Papaya plant is a medicinal plant which became popular for the treatment of dengue fever due to its property. Considering the current medicinal importance of the papaya plant, the present study was aimed at microwave assisted extraction of phenolic content from papaya leaf using ethanol, water as solvent and investigate their antioxidant potential. In order to compare the extraction efficiency of phenolic compounds, conventional extraction and microwave assisted extraction method was used to prepare the extracts. Then extracts were subjected to preliminary phytochemical analysis followed by the estimation of total phenolic content by using Folin-Ciocalteu method. Antioxidant activity was investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. The alcoholic and aqueous extracts of papaya leaf showed the presence of steroids, alkaloids, saponins, carbohydrates, phenolic compounds by preliminary phytochemical analysis. FTIR spectrum of both aqueous and ethanolic extract showed characteristic peak at 3314.62 cm-1, 1635 cm-1 which provide evidence for presence of phenolic compounds. The total phenolic content of the alcoholic and aqueous leaf extracts from MAE was found to be 43.58mg and 80.58 mg/g papaya leaf powder of the Gallic acid equivalent (GAE), respectively. Aqueous solvent was found to be suitable for extraction of phenolic content from papaya leaf and Microwave assisted extracts showed higher phenolic content and therefore potential antioxidant activity. Therefore, papaya leaf is a good candidate to be used as a natural antioxidant for the treatment of various diseases.

scholarly journals Optimization of the microwave assisted extraction and biological activities of polyphenols from lemon verbena leaves

2021 ◽  
Vol 45 (1) ◽  
pp. 157-177
Author(s):  
Zohra Touati ◽  
Menana Guemghar ◽  
Kenza Bedjaoui ◽  
Nabila Emilia Djerrada ◽  
Kahina Djaoud ◽  
...  
Keyword(s):  
Irradiation Time ◽  
Biological Activities ◽  
Reducing Power ◽  
Biological Properties ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Disk Diffusion Assay ◽  
Lemon Verbena

The present study aims to optimize the extraction of phenolics by microwave-assisted extraction (MAE) using the response surface methodology (RSM), from Lemon verbena leaves. The optimized extract was tested for its antioxidant activity using two methods (DPPH and reducing power) and its antibacterial efficiency by using disk diffusion assay and broth microdilution, against two Gram-negative (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853) and two Gram-positive (Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 6633) strains. Under the optimized conditions (40% (v/v): of ethanol concentration, 188 s of irradiation time, 600 W of microwave power and 1:40 g/mL of solid-to-liquid ratio) the total phenolic content (TPC) was 67.87±1.61 mg GAE/g DW. The IC50 of the extract was 139.65±1.44 µg/mL and 56.60±2.79 µg/mL for DPPH inhibition and reducing power, respectively. The best antibacterial activity was shown by the extract obtained by MAE with lower MBC (1.56 to 18.75 mg/mL) and MBC/MIC ratio. Lemon verbena extract can be used as an ingredient in cosmetics, food supplements and herbal medicinal products due to its interesting biological properties.

scholarly journals Extraction of Anthocyanins and Total Phenolic Compounds from Açai (Euterpe oleracea Mart.) Using an Experimental Design Methodology. Part 3: Microwave-Assisted Extraction

Agronomy ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 179 ◽  
Author(s):  
María José Aliaño-González ◽  
Marta Ferreiro-González ◽  
Estrella Espada-Bellido ◽  
Ceferino Carrera ◽  
Miguel Palma ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolic ◽  
Solvent Ratio ◽  
Microwave Assisted Extraction ◽  
Euterpe Oleracea ◽  
Total Phenolic Compounds ◽  
Real Samples ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Relative Standard

In this work, two methods based on microwave-assisted extraction techniques for the extraction of both anthocyanins and total phenolic compounds from açai have been developed. For that, a full factorial design (Box-Behnken design) has been used to optimize the following four variables: solvent composition (25–75% methanol in water), temperature (50–100 °C), pH (2–7), and sample/solvent ratio (0.5 g: 10 mL–0.5 g: 20 mL). The anthocyanins and total phenolic compounds content have been determined by ultra high-pressure liquid chromatography and Folin-Ciocalteu method, respectively. The optimum conditions for the extraction of anthocyanins were 38% MeOH in water, 99.63 °C, pH 3.00, at 0.5 g: 10 mL of ratio, while for the extraction of total phenolic compounds they were 74.16% MeOH in water, 99.14 °C, pH 5.46, at 0.5 g: 20 mL of ratio. Both methods have shown a high repeatability and intermediate precision with a relative standard deviation lower than 5%. Furthermore, an extraction kinetics study was carried out using extraction periods ranging from 2 min until 25 min. The optimized methods have been applied to açai-containing real samples. The results with such real samples have confirmed that both methods are suitable for a rapid and reliable extraction of anthocyanins and total phenolic compounds.

scholarly journals Optimization of Microwave-Assisted Extraction for the Recovery of Bioactive Compounds from the Chilean Superfruit (Aristotelia chilensis (Mol.) Stuntz)

Agronomy ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 240 ◽  
Author(s):  
Mercedes Vázquez-Espinosa ◽  
Estrella Espada-Bellido ◽  
Ana V. González de Peredo ◽  
Marta Ferreiro-González ◽  
Ceferino Carrera ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Total Phenolics ◽  
Total Phenolic ◽  
Microwave Assisted Extraction ◽  
Total Phenolic Compounds ◽  
Intermediate Precision ◽  
Microwave Assisted ◽  
Assisted Extraction ◽  
Aristotelia Chilensis ◽  
Maqui Berry

Maqui berry (Aristotelia chilensis) is being explored in the food industry, and is considered to be one of the healthiest berries due to its bioactive components and high commercial value. Microwave-assisted extraction (MAE) was developed for the determination of total phenolic compounds and anthocyanins from maqui. A Box–Behnken experimental design was employed in conjunction with a response surface methodology to optimize the conditions based on 27 different experiments. The extractions were carried out with four factors (i.e., methanol percentage, pH, temperature, and solvent volume:sample mass ratio), and two responses—total phenolics and anthocyanins. Temperature and methanol percentage were found to be the most influential parameters for total phenolic compounds and anthocyanins, respectively. The optimum MAE conditions were: 65% MeOH in water at pH 2, temperature of 100 °C, and a ratio of 10:0.5 for total phenolics; and 60% MeOH in water at pH 2, temperature of 50 °C, and a ratio of 14:0.5 for anthocyanins. Kinetics assays were carried out and an optimum time of only 2 min was identified for the extractions. Repeatability and intermediate precision were also evaluated, and coefficients of variation below 5% were obtained. The new methods were successfully applied to a foodstuff made with maqui.

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