Bacillus spp. COMO PROMOTORES DE RESISTÊNCIA VEGETAL: SECA, A SALINIDADE E A PATÓGENOS HABITANTES DO SOLO EM ÁREAS ÁRIDAS

2020 ◽  
Vol 4 (5) ◽  
pp. 1
Author(s):  
Thiago Costa Ferreira ◽  
Ana Lúcia Araújo Cunha
Keyword(s):  
Bacillus Spp

<p>A utilização de isolados de <em>Bacillus</em> spp. na agricultura têm sido atrativo ao mercado, pois estes microrganismos podem promover a resistência de plantas a fatores como seca, salinidade e patógenos do solo. Assim sendo, o objetivo dessa pesquisa foi agrupar conhecimentos sobre a utilização de isolados de <em>Bacillus</em> spp. como promotor de resistência vegetal à seca, a salinidade e a patógenos habitantes do solo, com ênfase a áreas com aridez. Logo, foi efetuada uma busca em periódicos e bases indexadas com a utilização dos seguintes marcadores: <em>Bacillus</em>, seca, salinidade e fitopatógenos habitantes do solo. De acordo com os dados reunidos pode afirmar que isolados de <em>Bacillus</em> spp. podem ser úteis na construção de uma agricultura sustentável em áreas com aridez na presença dos fatores: seca, salinidade e presença de patógenos habitantes do solo e que o aporte me matéria orgânica pode ser a melhor maneira de suplementar esta comunidade em áreas áridas. Este é um campo de estudo vasto e pouco explorado, portanto, maiores pesquisas neste sentido podem ser realizadas afim de promover mais conhecimentos e gerar desenvolvimento sustentável a regiões áridas.</p>

PHÂN LẬP VÀ TUYỂN CHỌN CÁC CHỦNG BACILLUS ỨC CHẾ VI KHUẨN VIBRIO PARAHAEMOLYTICUS GÂY BỆNH TÔM CHẾT SỚM Ở TỈNH THỪA THIÊN HUẾ

2015 ◽  
Vol 100 (1) ◽  
Author(s):  
Nguyễn Thị Bích Đào ◽  
Trần Quang Khánh Vân ◽  
Nguyễn Văn Khanh ◽  
Nguyễn Quang Linh
Keyword(s):  
Bacillus Subtilis ◽  
Vibrio Parahaemolyticus ◽  
Bacillus Amyloliquefaciens ◽  
Bacillus Spp ◽  
Bacillus Subtilis B1

Khi tình hình bệnh hội chứng tôm chết sớm (EMS) đã gây thiệt hại vô cùng to lớn đối với Nuôi trồng thủy sản thì các giải pháp được đề nghị và áp dụng nhằm hạn chế dịch bệnh. Trong đó, việc tìm hiểu và đưa vi khuẩn có lợi để cạnh tranh và ức chế loài vi khuẩn gây bệnh rất được quan tâm, được cho là giải pháp có nhiều triển vọng phù hợp với điều kiện môi trường, đảm bảo sức khỏe cho con người, cũng như hạn chế được dịch bệnh. Đặc biệt, đưa vi khuẩn Bacillus spp. qua đường tiêu hóa của tôm ngay từ khi mới thả đã hạn chế được mật độ vi khuẩn Vibrio. Nghiên cứu này đã phân lập được các chủng Bacillus subtilis B1, Bacillus subtilis B2, Bacillus amyloliquefaciens B4và thử khả năng đối kháng với vi khuẩn Vibrio parahaemolyticus V1 ở các nồng độ 103, 104, 105, 106 CFU theo dõi ở các thời điểm 6h, 12h, 24h, 48h và 72h. Kết quả cho thấy cả ba chủng vi khuẩn Bacillus trên phân lập được đều có khả năng ức chế tốt vi khuẩn Vibrio parahaemolyticus V1, trong đó vi khuẩn Bacillus amyloliquefaciens B4 làtốt nhất với đường kính vòng kháng khuẩn 52,67 ± 4,31mm ở thời điểm 48h; hai chủng Bacillus subtilis B1, Bacillus subtilis B2 lầnlượt là  49,67 ± 3,15 mm, 44,07 ± 5,19 mm, với mức sai số có ý nghĩa thống kê p < 0,05.

Assessment of Bacterial Contamination of Orthodontic Arch wire

2019 ◽  
Vol 31 (1) ◽  
pp. 48-51
Author(s):  
Suha S Hassan ◽  
Nidhal H. Ghaib ◽  
Batool H Al-Ghurabi
Keyword(s):  
Bacterial Contamination ◽  
Microbial Contamination ◽  
Ethylenediaminetetraacetic Acid ◽  
Bacterial Species ◽  
Brain Heart Infusion ◽  
Control Groups ◽  
Dental Practitioners ◽  
Significant Difference ◽  
Bacillus Spp ◽  
Droplet Transmission

Background: The microorganisms can impend the life of health care professional and particularly the dental practitioners. They can be transmitted by different ways like airborne and droplet transmission. The current study was carried out to identify whether the arch wires that received from the manufactures are free from microbial contamination and to determine the bacterial species attached to the arch wires. Materials and Methods: This study involved eighty samples, consisted of two types of arch wires (nitinol and stainless-steel) from four companies (3M, G&H, Jiscop, OrthoTechnology). These wires inserted in a plane tube that contains 10 -ml of (Tris [tris(hydroxymethyl)aminomethane] and EDTA (ethylenediaminetetraacetic acid) tris-EDTA and brain heart infusion (BHI) broth. A 0.1 ml was withdrawn from the tube and spread on agar plates. The control groups consist of 16 plane tube (8 tubes with tris-EDTA and other 8 tubes with (BHI). Results: Microbial sampling yielded growth from 5 of the 80 arch wires. The predominant bacteria that isolated were Bacillus spp. No growth was recovered from 75 of the samples and from controls. The bacteria were isolated by BHI reagent and no growth was observed by tris-EDTA reagent with statistically significant difference (P<0.05). The Bacillus spp. found only in the G&H and Jiscop companies, however, no statistically significant difference was found among them (P>0.05). With regard to the presence and distribution of bacteria according to the types of wires, the present results clarified that cases of contamination with Bacillus spp. were found in the nitinol arch wires with statistically significant difference (P<0.05). Conclusions: The results of the current study revealed low count of bacterial contamination in the two types of companies (G&H and Jiscop). Not all materials that received from the manufactures are free from contamination and an effective sterilization regimen is needed to avoid cross-contamination.

Risk analysis of Bacillus spp. isolated from cured pork sausages

2003 ◽  
Author(s):  
M. Morea ◽  
A. Matarante ◽  
F. Baruzzi ◽  
A. Abbrescia
Keyword(s):  
Risk Analysis ◽  
Bacillus Spp ◽  
Pork Sausages

Exploration of Endophytes from Alfalfa (Medicago sativa L.) as Biocontrol Agents

2020 ◽  
pp. 1-8
Author(s):  
Beilei Wu ◽  
Beilei Wu ◽  
Mei Li ◽  
Xiaoli Chen ◽  
Xiliang Jiang ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Root Rot ◽  
Agricultural Production ◽  
Biocontrol Agents ◽  
Bacillus Spp ◽  
Medicago Sativa L ◽  
The Common ◽  
Seedling Germination ◽  
Germination Experiments ◽  
Alfalfa Root

Endophytes are increasingly investigated as biocontrol agents for agricultural production. The identification of new endophytes with high effectiveness against plant disease is very important. A total of 362 strains of endophytes, including fungi, bacteria, and actinomycete, were isolated from alfalfa (Medicago sativa L.) collected in Hebei, Inner Mongolia and Ningxia provinces of China. The three strains of endophytic bacteria (NA NX51R-5, NA NX90R-8, and NA NM1S-1) with strong biocontrol capability with >50% effectiveness were screened against the common alfalfa root rot pathogen Fusarium oxysporum F. sp. medicaginis in alfalfa seedling germination experiments on MS medium and pot experiments. Using phylogenetic analysis, the isolates of NA NM1S-1 and NA NX51R-5 were identified as Bacillus spp. by 16S rDNA, while NA NX90R-8 was found to be Pseudomonas sp.

The Effectiveness of Amoxicillin and Cefalexin in Two Species from Staphylococcus and Bacillus Isolated from Bovine Mastitis in Sudan

2019 ◽  
Author(s):  
Reem Rabie Mohammed Salih
Keyword(s):  
Bacillus Cereus ◽  
Bovine Mastitis ◽  
Bacillus Coagulans ◽  
Staph Aureus ◽  
Milk Samples ◽  
Sensitivity Tests ◽  
Bacillus Spp ◽  
Staphylococcus Spp

In this study 60 milk samples collected from mastitic cows to isolates and identify bacterial agents. The percentage of isolates was as follows: Staphylococcus spp 52%, Bacillus spp 26%, Enteroccocispp 3%; microccus 3%, Nesseria 2%, Branhamella 3%, Clostridium 1%, Bordetella 2%, Enterobacteria 3%, Aeromonas 4%, Fusobacterium 1%. In sensitivity tests used two antibiotics Amoxicillin and Cephalexin against two highest isolates in this study (Staph spp and Bacillus spp and found: Staph. aureus and Staph. hyicus were resistant to Amoxicllin and cephlaxin relatively, Bacillus coagulans and Bacillus cereus were susceptible to Amoxicllin and cephlaxin.

Impact of nanochitosan and Bacillus spp. on health, productivity and defence response in Zea mays under field condition

3 Biotech ◽  
2021 ◽  
Vol 11 (5) ◽  
Author(s):  
Parul Chaudhary ◽  
Priyanka Khati ◽  
Saurabh Gangola ◽  
Ashish Kumar ◽  
Rajeew Kumar ◽  
...  
Keyword(s):  
Zea Mays ◽  
Field Condition ◽  
Defence Response ◽  
Bacillus Spp

scholarly journals A Novel Microbial Zearalenone Transformation through Phosphorylation

Toxins ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 294
Author(s):  
Yan Zhu ◽  
Pascal Drouin ◽  
Dion Lepp ◽  
Xiu-Zhen Li ◽  
Honghui Zhu ◽  
...  
Keyword(s):  
Microbial Transformation ◽  
Acid Tolerance ◽  
Transformation Product ◽  
The United States ◽  
Corn Silage ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
High Concentrations ◽  
The 16S Rrna Gene ◽  
Bacillus Strains

Zearalenone (ZEA) is a mycotoxin widely occurring in many agricultural commodities. In this study, a purified bacterial isolate, Bacillus sp. S62-W, obtained from one of 104 corn silage samples from various silos located in the United States, exhibited activity to transform the mycotoxin ZEA. A novel microbial transformation product, ZEA-14-phosphate, was detected, purified, and identified by HPLC, LC-MS, and NMR analyses. The isolate has been identified as belonging to the genus Bacillus according to phylogenetic analysis of the 16S rRNA gene and whole genome alignments. The isolate showed high efficacy in transforming ZEA to ZEA-14-phosphate (100% transformation within 24 h) and possessed advantages of acid tolerance (work at pH = 4.0), working under a broad range of temperatures (22–42 °C), and a capability of transforming ZEA at high concentrations (up to 200 µg/mL). In addition, 23 Bacillus strains of various species were tested for their ZEA phosphorylation activity. Thirteen of the Bacillus strains showed phosphorylation functionality at an efficacy of between 20.3% and 99.4% after 24 h incubation, suggesting the metabolism pathway is widely conserved in Bacillus spp. This study established a new transformation system for potential application of controlling ZEA although the metabolism and toxicity of ZEA-14-phosphate requires further investigation.

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