Microbial degradation of an industrial azo-dye and FT-IR analysis

The presence of dyes in wastewaters from the textile industry, even in concentrations of less than 1 mg/l, significantlyaffects the aesthetic properties and transparence degree of public effluents, with direct repercussions on theenvironment. Cerioporus squamosus White-Rot-Fungi (WRF) strain was used for bio-augmentation of MBBR carriers(consisting of a mix of 88% High Density Polyethylene, 5% talcum and 7% cellulose). Cerioporus squamosus, also oftenencountered as Polyporus squamosus, is a basidiomycete bracket fungus, able to cause “white rot” on decaying wood.The bio-functionalized carriers were used for treatment of a synthetic wastewater sample, of Bemacid ROT (Bezema)azo-dye. Azoic dyes represent one of the most important classes of synthetic dyes used in the textile industry,accounting for over 60–70% of the dyes used in this industry. In the case of reactive groups of azo dyes (-N=N-), dueto the low degrees of fixation on the fiber, there are losses of dyes in solution of up to 50%. Infrared spectral (FT-IR)analysis was carried out for determination of functional groups involved in biodegradative processes. Thus, the obtainedIR spectra, different from those of initial Bemacid ROT dye, the disappearance or decrease of the signal specific to azoicbonds from the initial sample, the formation of new functional groups, the disappearance of intermolecular hydrogenbonds simultaneously with increase of transmittance values for amino groups, resulted in highlighting the degradationof Bemacid ROT dye by the bio-augmented HDPE carriers.

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Synthetic dyes decolourisation by white-rot fungi: Development of original microtitre plate method and screening

Enzyme and Microbial Technology ◽

10.1016/j.enzmictec.2007.07.023 ◽

2008 ◽

Vol 42 (2) ◽

pp. 97-106 ◽

Cited By ~ 22

Author(s):

M. Lucas ◽

V. Mertens ◽

A.-M. Corbisier ◽

S. Vanhulle

Keyword(s):

White Rot Fungi ◽

White Rot ◽

Synthetic Dyes ◽

Microtitre Plate ◽

Plate Method

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Phytochemical screening and in-vitro antibacterial and DPPH free radical scavenging activities of methanol extract of root of Combretum album Pers.

Plant Science Today ◽

10.14719/pst.2021.8.4.1158 ◽

2021 ◽

Vol 8 (4) ◽

Author(s):

Sunanda Burman ◽

Goutam Chandra

Keyword(s):

Functional Groups ◽

Methanol Extract ◽

Methanolic Extract ◽

Radical Scavenging ◽

Chloroform Fraction ◽

Active Ingredients ◽

Ic50 Value ◽

Ft Ir ◽

Ir Analysis

The objective of the study was to investigate in-vitro antibacterial and antioxidant activities of methanolic extract of Combretum album Pers. root. In brief antibacterial efficacies of methanol extract and its petroleum ether fraction (MePET), chloroform fraction (MeCH), ethyl acetate fraction (MeEA) and water fraction (MeAQ) were determined by agar well diffusion assay along with Minimum Inhibitory Concentrations (MICs). In-vitro antioxidant efficacies were evaluated by DPPH radical scavenging method. Preliminary phytochemical assay, Fourier Transform Infrared Spectroscopy and Gas Chromatography-Mass Spectroscopy analyses were employed to detect the plausible active ingredients. Methanol extract showed broad spectrum antibacterial activity having highest inhibition zone against Staphylococcus aureus MTCC 2940 (21.67±0.58 mm) and MICs ranged from100 µg/ml to 250 µg/ml. The scavenging activity of methanol extract of root of C. album was concentration dependant and IC50 value was 136.08 µg/ml. The lowest MIC (5 µg/ml) was noted with MeCH against B. subtilis (MTCC 441). MeCH showed highest antioxidant activity with an IC50 value of 12.98 ?g/mL and MePET, MeEA, MeAQ and Ascorbic acid presented antioxidant potential with IC50 values of 16.10, 15.07, 17.44 and 13.40??g/mL respectively. Preliminary phytochemical tests and FT-IR analysis revealed presence of various phytochemicals and functional groups like hydroxyl, carboxylic acids, amidines, amines, aromatics and esters. Three compounds were elucidated from bioactive TLC fraction of MeCH in GC-MS analysis supported by presence of various functional groups in MeCH as detected in FT-IR analysis. It is concluded that methanolic extract of C. album root is a potential antibacterial and antioxidant agent where presence of N-[5-[4-pyridinyl)-1H-1,2,4-triazol-3-yl]-benzamide (compound 1), pyruvic acid (compound 2) and methyloacetone (compound 3) are responsible as possible active ingredients.

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Bio-degradation of Azo Dye Acid Orange-10 by a New Isolate of Bacillus subtilis Isolated from Soil Sample around Textile Industry in South Karnataka

Biosciences Biotechnology Research Asia ◽

10.13005/bbra/2875 ◽

2021 ◽

Vol 17 (4) ◽

pp. 707-716

Author(s):

Hema Gunti ◽

Vyshali Venkatappa Maruthiramaih ◽

Tippeswamy Boreddy Shivanandappa

Keyword(s):

Bacillus Subtilis ◽

Textile Industry ◽

Azo Dye ◽

Industrial Effluent ◽

Inoculum Size ◽

Soil Samples ◽

Synthetic Dyes ◽

Acid Orange ◽

Acid Orange 10 ◽

Static Conditions

Untreated effluents from the textile industry affect aquatic life irreversibly. Synthetic dyes not only change the color of water resources but also make them hazardous.The main objective of the study was to evaluate the decolorizing potential of a new isolate of Bacillus subtilis from soil samples contaminated with industrial effluent in and around textile industrial area in South Karnataka. This isolate of Bacillus subtilis has high decolorizing potential and took only 24 hrs for complete decolorization of acid orange-10 azo dye at 200ppm. Different parameters like temperature, pH, aeration, dye concentration and inoculum size were optimized for complete decolorization of Acid orange-10 azo dye by this isolate of Bacillus subtilis. The dye was completely decolorized at 400C within 24 hrs and it was capable of decolorizing 700 ppm dye in 72 hrs. Optimum pH was found to be 8.5 and maximum decolorization was achieved under static conditions. As the inoculum size increased, the time taken for complete decolorization of Acid orange-10 dye was decreased from 36 hrs at 1% to 16 hrs at 10% of inoculum size. The new isolate decolorizes 100 ppm of dye completely (i.e.100%) within 12hrs of incubation. The time taken for the complete decolorization increased with increase in the concentration of Acid orange-10 azo dye. In conclusion, the new isolate of Bacillus subtilis from soil samples contaminated with textile industrial effluent was found to be a potential candidate for decolorization of Acid orange-10 azo dye in textile effluents.

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Extraction of Dyes from Sunflower Petal and Their Fourier Transform Infrared Characterization

Organic Polymer Material Research ◽

10.30564/opmr.v3i1.2586 ◽

2022 ◽

Vol 3 (2) ◽

Author(s):

Gboyega Oluwaseun Oyeleke ◽

Ibraheem Abimbade Abdulazeez ◽

Ajisola Agnes Adebisi ◽

Kehinde Nasiru Oyekanmi ◽

Segun Olaitan Akinbode

Keyword(s):

Fourier Transform ◽

Helianthus Annuus ◽

Functional Groups ◽

Methanolic Extract ◽

Fourier Transform Infrared ◽

Infrared Spectrometry ◽

Fourier Transform Infrared Spectrometry ◽

Naoh Solution ◽

Ft Ir ◽

Ir Analysis

Three solvents of different polarities (water, methanol and 1% NaOHsolution) were used to extract dyes that produced different shades fromdried sunflower (Helianthus annuus) petal. The extraction proceduresusing different solvent types were carried out separately. The dye extractswere thereafter subjected to Fourier Transform Infrared Spectrometry(FT-IR) analysis for characterization in terms of functional groups. Theintensities of the extracted dyes on the shade of colours obtained on piecesof cotton material varied from yellow in methanolic extract to light yellowin aqueous and black in 1% NaOH solution extracts. The results obtainedfrom the FT-IR analysis revealed the presence of several useful functionalgroups such as N-H, C=H, O-H and C=O in the extracts.

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Inducing laccase activity in white rot fungi using copper ions and improving the efficiency of azo dye treatment with electricity generation using microbial fuel cells

Chemosphere ◽

10.1016/j.chemosphere.2019.125304 ◽

2020 ◽

Vol 243 ◽

pp. 125304 ◽

Cited By ~ 7

Author(s):

Shu-Hui Liu ◽

Shen-Long Tsai ◽

Pei-Yu Guo ◽

Chi-Wen Lin

Keyword(s):

Fuel Cells ◽

Microbial Fuel Cells ◽

Azo Dye ◽

Electricity Generation ◽

Laccase Activity ◽

Copper Ions ◽

White Rot Fungi ◽

White Rot

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Phytochemical and antimicrobial analyses of Plectranthus amboinicus leaf extracts

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.01103 ◽

2021 ◽

pp. 6379-6384

Author(s):

M.S. Sindhu ◽

Poonkothai M.

Keyword(s):

Antimicrobial Activity ◽

Petroleum Ether ◽

Functional Groups ◽

Leaf Extract ◽

Methanol Extract ◽

Leaf Extracts ◽

Fungal Isolates ◽

Ft Ir ◽

Ir Analysis ◽

Plectranthus Amboinicus

The present study is to evaluate the phytochemical constituents and antimicrobial activity of Plectranthus amboinicus leaf extract using petroleum ether, methanol and water as solvents. The antimicrobial activity of P. amboinicus leaf extracts were examined against the selected bacterial and fungal isolates namely Escherichia coli, Staphylococcus aureus and Aspergillus niger, Candida albicans using agar well diffusion method. FT –IR analysis was performed to identify the presence of functional groups in the methanol extract. The results of the study revealed the presence of distinctive active secondary metabolites in the tested leaf extract. The results revealed that the methanol extract exhibited maximum antimicrobial activity against the tested bacterial and fungal isolates when compared with aqueous and petroleum ether extracts. The FT-IR analysis reveals the presence of functional groups such as hydroxyl, amines, alkynes, ketones and carboxylic acid in the methanolic extract of P. amboinicus. Thus, P. amboinicus leaves are effective against the selected microbes and recommends that the plant derived phytochemicals are comparatively safer than synthetic alternative, thereby contributing insightful remedial benefits for the treatment of diseases.

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