scholarly journals Bio-degradation of Azo Dye Acid Orange-10 by a New Isolate of Bacillus subtilis Isolated from Soil Sample around Textile Industry in South Karnataka

2021 ◽  
Vol 17 (4) ◽  
pp. 707-716
Author(s):  
Hema Gunti ◽  
Vyshali Venkatappa Maruthiramaih ◽  
Tippeswamy Boreddy Shivanandappa
Keyword(s):  
Bacillus Subtilis ◽  
Textile Industry ◽  
Azo Dye ◽  
Industrial Effluent ◽  
Inoculum Size ◽  
Soil Samples ◽  
Synthetic Dyes ◽  
Acid Orange ◽  
Acid Orange 10 ◽  
Static Conditions

Untreated effluents from the textile industry affect aquatic life irreversibly. Synthetic dyes not only change the color of water resources but also make them hazardous.The main objective of the study was to evaluate the decolorizing potential of a new isolate of Bacillus subtilis from soil samples contaminated with industrial effluent in and around textile industrial area in South Karnataka. This isolate of Bacillus subtilis has high decolorizing potential and took only 24 hrs for complete decolorization of acid orange-10 azo dye at 200ppm. Different parameters like temperature, pH, aeration, dye concentration and inoculum size were optimized for complete decolorization of Acid orange-10 azo dye by this isolate of Bacillus subtilis. The dye was completely decolorized at 400C within 24 hrs and it was capable of decolorizing 700 ppm dye in 72 hrs. Optimum pH was found to be 8.5 and maximum decolorization was achieved under static conditions. As the inoculum size increased, the time taken for complete decolorization of Acid orange-10 dye was decreased from 36 hrs at 1% to 16 hrs at 10% of inoculum size. The new isolate decolorizes 100 ppm of dye completely (i.e.100%) within 12hrs of incubation. The time taken for the complete decolorization increased with increase in the concentration of Acid orange-10 azo dye. In conclusion, the new isolate of Bacillus subtilis from soil samples contaminated with textile industrial effluent was found to be a potential candidate for decolorization of Acid orange-10 azo dye in textile effluents.

scholarly journals Degradation of Reactive Black 5 dye by a newly isolated bacterium Pseudomonas entomophila BS1

2016 ◽  
Vol 62 (3) ◽  
pp. 220-232 ◽  
Author(s):  
Sana Khan ◽  
Abdul Malik
Keyword(s):  
Textile Industry ◽  
Azo Dye ◽  
Degradation Pathway ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Flavin Mononucleotide ◽  
Reactive Black 5 ◽  
Spectrometry Analysis ◽  
Rdna Sequencing ◽  
Static Conditions

The textile and dye industries are considered as one of the major sources of environmental pollution. The present study was conducted to investigate the degradation of the azo dye Reactive Black 5 (RB 5) using a bacterium isolated from soil samples collected around a textile industry. The bacterial strain BS1 capable of degrading RB 5 was isolated and identified as Pseudomonas entomophila on the basis of 16S rDNA sequencing. The effects of different parameters on the degradation of RB 5 were studied to find out the optimal conditions required for maximum degradation, which was 93% after 120 h of incubation. Static conditions with pH in the range of 5–9 and a temperature of 37 °C were found to be optimum for degrading RB 5. Enzyme assays demonstrated that P. entomophila possessed azoreductase, which played an important role in degradation. The enzyme was dependent on flavin mononucleotide and NADH for its activity. Furthermore, a possible degradation pathway of the dye was proposed through gas chromatography – mass spectrometry analysis, which revealed that the metabolic products were naphthalene-1,2-diamine and 4-(methylsulfonyl) aniline. Thus the ability of this indigenous bacterial isolate for simultaneous decolorization and degradation of the azo dye signifies its potential application for treatment of industrial wastewaters containing azo dyes.

scholarly journals Bioremediation of methylene blue dye using Bacillus subtilis MTCC 441

2017 ◽  
Vol 75 (7) ◽  
pp. 1572-1583 ◽  
Author(s):  
Ganta Upendar ◽  
Susmita Dutta ◽  
Pinaki Bhattacharya ◽  
Abhishek Dutta
Keyword(s):  
Methylene Blue ◽  
Bacillus Subtilis ◽  
Textile Industry ◽  
Immobilized Cells ◽  
Fixed Bed ◽  
Inoculum Size ◽  
Blue Dye ◽  
Influent Concentration ◽  
Operating Variables ◽  
Industry Effluent

Methylene blue (MB) commonly found in the textile industry effluent has been chosen as a model dye to investigate bioremediation using Bacillus subtilis MTCC 441. Both free cells and calcium alginate immobilized cells have been used to remove MB from the effluent. The operating variables of initial concentration of dye (20–60 mg/L), inoculum size (4–8%) and temperature (25–35 °C) have been varied judiciously during the kinetic study in a batch contactor. A maximum removal of 91.68% is obtained when 20 mg/L MB solution was inoculated with 8% inoculum and cultured for 6 h at 30 °C. Continuous removal of MB has been studied in a fixed bed contactor using immobilized cells as packing materials. Influent concentration (10–30 mg/L) was varied and breakthrough parameters have been determined. With increase in influent concentration from 10 mg/L to 30 mg/L, percentage removal of dye decreases from 72.44% to 49.62%.

scholarly journals Enhanced decolourization of congo red dye under submerged fermentation (SMF) process by newly isolated Bacillus subtilis SPR42

2011 ◽  
Vol 3 (1) ◽  
pp. 51-53 ◽  
Author(s):  
Baljeet Singh Saharan ◽  
Poonam Ranga
Keyword(s):  
Bacillus Subtilis ◽  
Congo Red ◽  
Azo Dye ◽  
Bacterial Biomass ◽  
Phenotypic Characterization ◽  
Textile Processing ◽  
Sequence Comparisons ◽  
Congo Red Dye ◽  
Static Conditions ◽  
Red Dye

Studies were carried out on the decolourization of textile azo dye using newly isolated aerobic bacterial culture. Among the 58 strains of aerobic bacteria isolated from soil contaminated with textile industry (Shivalik polymer Ltd. Faridabad) effluent, three showed remarkable ability in decolourizing the widely utilized azo dye (Congo Red). These strains also readily grew in and decolourized the high concentrations of dye (100 mgL -1). The aerobic bacterial isolate SPR42, was able to decolourize the Congo Red dye at a concentration of 100 mgL -1 upto 94% within 24 hrs at static conditions. The temperature and pH for optimum growth and activity of the isolate were reported as 37 oC and 8.5, respectively. The colorless bacterial biomass after decolourization suggested that decolourization was due to biodegradation, rather than inactive surface adsorption. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicate that the strain SPR42 identified as Bacillus subtilis. This isolate can be a potential strain for biological treatment of effluents of TPI (Textile Processing Industry).

scholarly journals Biodegradation of Basic Yellow Auramine- O Dye using Staphylococcus sp. Isolated from Textile Industry Effluent

2021 ◽  
Vol 14 (4) ◽  
pp. 1571-1576
Author(s):  
Anil R. Shet
Keyword(s):  
Carbon Source ◽  
Textile Industry ◽  
Dye Decolorization ◽  
Inoculum Size ◽  
Synthetic Dyes ◽  
Bacterial Isolates ◽  
Gram Staining ◽  
Initial Ph ◽  
Auramine O ◽  
Industry Effluent

Due to the increased use of synthetic dyes in various industries, there is an increased disposal of wastewater containing harmful dyes. These, in turn, have affected plants, animals, and humans. The physical and chemical methods of dye decolorization have failed to degrade the synthetic dyes in industrial effluents completely. The microbial decolorization is better due to its versatility, dynamic metabolism, and potential machinery of enzymes. This study aimed to degrade basic yellow dye auramine O by bacteria isolated from textile industry effluent. In this regard, five bacterial strains were isolated and screened from a soil sample taken from textile industry effluent. The initial physical and biochemical characterization of the bacterial isolates 1 and 2 indicated catalase test-positive, starch test-negative, motility agar test-negative, gram staining test-positive, and morphology-bacillus. The bacterial isolates 3, 4, and 5 indicated oxidase test-negative, urease test-positive, gram staining test-negative, and morphology-staphylococcus. All the isolates were further subjected to a screening test, where isolate 5 showed maximum dye decolorization of 98.9% in 96 h. The biodegradation of dye was optimized for different values of initial pH (4-10), inoculum size (2% -10%), initial dye concentration (50 mgL-1 to400 mgL-1), carbon source (glucose, fructose, xylose, starch and lactose) and nitrogen source (peptone, ammonium sulphate, yeast extract, ammonium nitrate and urea). Maximum dye decolorization was observed for initial dye concentration of 200 mgL-1, initial pH of 6, inoculum size of 10%, yeast extract as nitrogen source, and glucose as carbon source. Therefore, dye degradation by bacteria can be used as a potential method for auramine O dye treatment.

Axo-dye biodegradation under anoxic conditions

1996 ◽  
Vol 34 (5-6) ◽  
pp. 495-500 ◽  
Author(s):  
U. Zissi ◽  
G. Lyberatos
Keyword(s):  
Bacillus Subtilis ◽  
Azo Dyes ◽  
Textile Industry ◽  
Azo Dye ◽  
Manufacturing Industry ◽  
Dye Degradation ◽  
Synthetic Medium ◽  
Terminal Electron Acceptor ◽  
Anoxic Conditions ◽  
Nitrogen Double Bond

Biological oxidation of azo-dyes is important for wastewater treatment. Azo-dyes are synthetic organic colorants that exhibit great structural variety. A large majority of these dyes are released into the environment. The textile industry and dyestuff manufacturing industry are two major sources of released azodyes. In the present study, we focus on the anoxic degradation of a disperse azo-dye, p-aminoazobenzene (pAAB), a simple azo-dye, by a pure culture of Bacillus subtilis, growing on a synthetic medium. Bacillus subtilis is a bacterium capable of using nitrate and/or nitrite as terminal electron acceptor under anoxic conditions. This bacterium lacks the capability for fermentation. The degradation of p-aminoazobenzene by Bacillus subtilis was examined through batch experiments in order to elucidate the mechanism of dye degradation. The results proved that Bacillus subtilis co-metabolizes p-aminoazobenzene under denitrifying conditions, in the presence of glucose as carbon source, producing aniline and p-phenylenediamine as the nitrogen-nitrogen double bond is broken.

scholarly journals BIODEGRADASI ZAT WARNA ACID ORANGE 7 MENGGUNAKAN ENZIM JAMUR TIRAM PUTIH (PLEUROTUS OSTREATUS)

2018 ◽  
Vol 3 (1) ◽  
pp. 47
Author(s):  
Achmad Amiruddin ◽  
Hasri Hasri ◽  
Sudding Sudding
Keyword(s):  
Pleurotus Ostreatus ◽  
Textile Industry ◽  
Environmentally Friendly ◽  
Water Bodies ◽  
Oyster Mushroom ◽  
Synthetic Dyes ◽  
Acid Orange 7 ◽  
Acid Orange ◽  
Degradation Time

Synthetic dyes is most be usedin the textile industry because it is stable, easy to obtain, and cheap,the waste, however,is difficult to degrade, so it can cause contaminants in the waters and disrupt the aesthetics of water bodies. One of the alternative and environmentally-friendly methods that can be used to degrade dyestuff waste prior to discharge into the waters is by utilizing microorganisms as degrading agents. Therefore, this study aims to determine the ability of P.ostreatus fungus enzymes in degrading acid orange dyes 7 based on the variation of degradation time. The results showed that P.ostreatus fungus enzyme was able to degrade the orange acid 7 until 59.36%  Keywords: degradation, acid orange 7, white oyster mushroom (P.ostreatus)

scholarly journals Microbial degradation of an industrial azo-dye and FT-IR analysis

2020 ◽  
Vol 71 (06) ◽  
pp. 600-604
Author(s):  
OVIDIU IORDACHE ◽  
IOANA CORINA MOGA ◽  
ELENA-CORNELIA MITRAN ◽  
IRINA-MARIANA SANDULACHE ◽  
LUCIA-OANA SECAREANU ◽  
...  
Keyword(s):  
Functional Groups ◽  
Textile Industry ◽  
Azo Dye ◽  
White Rot Fungi ◽  
White Rot ◽  
Synthetic Wastewater ◽  
Wastewater Sample ◽  
Synthetic Dyes ◽  
Ft Ir ◽  
Ir Analysis

The presence of dyes in wastewaters from the textile industry, even in concentrations of less than 1 mg/l, significantlyaffects the aesthetic properties and transparence degree of public effluents, with direct repercussions on theenvironment. Cerioporus squamosus White-Rot-Fungi (WRF) strain was used for bio-augmentation of MBBR carriers(consisting of a mix of 88% High Density Polyethylene, 5% talcum and 7% cellulose). Cerioporus squamosus, also oftenencountered as Polyporus squamosus, is a basidiomycete bracket fungus, able to cause “white rot” on decaying wood.The bio-functionalized carriers were used for treatment of a synthetic wastewater sample, of Bemacid ROT (Bezema)azo-dye. Azoic dyes represent one of the most important classes of synthetic dyes used in the textile industry,accounting for over 60–70% of the dyes used in this industry. In the case of reactive groups of azo dyes (-N=N-), dueto the low degrees of fixation on the fiber, there are losses of dyes in solution of up to 50%. Infrared spectral (FT-IR)analysis was carried out for determination of functional groups involved in biodegradative processes. Thus, the obtainedIR spectra, different from those of initial Bemacid ROT dye, the disappearance or decrease of the signal specific to azoicbonds from the initial sample, the formation of new functional groups, the disappearance of intermolecular hydrogenbonds simultaneously with increase of transmittance values for amino groups, resulted in highlighting the degradationof Bemacid ROT dye by the bio-augmented HDPE carriers.

scholarly journals Microbial degradation of an industrial azo-dye and FT-IR analysis

2020 ◽  
Vol 71 (06) ◽  
pp. 600-604
Author(s):  
OVIDIU IORDACHE ◽  
IOANA CORINA MOGA ◽  
ELENA-CORNELIA MITRAN ◽  
IRINA-MARIANA SANDULACHE ◽  
LUCIA-OANA SECAREANU ◽  
...  
Keyword(s):  
Functional Groups ◽  
Textile Industry ◽  
Azo Dye ◽  
White Rot Fungi ◽  
White Rot ◽  
Synthetic Wastewater ◽  
Wastewater Sample ◽  
Synthetic Dyes ◽  
Ft Ir ◽  
Ir Analysis

The presence of dyes in wastewaters from the textile industry, even in concentrations of less than 1 mg/l, significantlyaffects the aesthetic properties and transparence degree of public effluents, with direct repercussions on theenvironment. Cerioporus squamosus White-Rot-Fungi (WRF) strain was used for bio-augmentation of MBBR carriers(consisting of a mix of 88% High Density Polyethylene, 5% talcum and 7% cellulose). Cerioporus squamosus, also oftenencountered as Polyporus squamosus, is a basidiomycete bracket fungus, able to cause “white rot” on decaying wood.The bio-functionalized carriers were used for treatment of a synthetic wastewater sample, of Bemacid ROT (Bezema)azo-dye. Azoic dyes represent one of the most important classes of synthetic dyes used in the textile industry,accounting for over 60–70% of the dyes used in this industry. In the case of reactive groups of azo dyes (-N=N-), dueto the low degrees of fixation on the fiber, there are losses of dyes in solution of up to 50%. Infrared spectral (FT-IR)analysis was carried out for determination of functional groups involved in biodegradative processes. Thus, the obtainedIR spectra, different from those of initial Bemacid ROT dye, the disappearance or decrease of the signal specific to azoicbonds from the initial sample, the formation of new functional groups, the disappearance of intermolecular hydrogenbonds simultaneously with increase of transmittance values for amino groups, resulted in highlighting the degradationof Bemacid ROT dye by the bio-augmented HDPE carriers.

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