scholarly journals CREATING OSTEOPLASTIC MATERIALS TO REPAIR JAW BONES DEFECTS

2020 ◽  
Vol 10 (4) ◽  
pp. 163-166
Author(s):  
Dmitry Kompantsev ◽  
Anna Chahirova ◽  
Ruslan Yusupov ◽  
Natalia Shabanova
Keyword(s):  
Bone Tissue ◽  
Animal Cell ◽  
Cell Types ◽  
Bone Tissue Regeneration ◽  
Laboratory Animals ◽  
Optimal Composition ◽  
Bioactive Materials ◽  
Jaw Bones ◽  
Defect Zone ◽  
Osteoplastic Material

In the course of our research we have developed a technology for fabricating an osteoplastic material from natural bone tissue. The obtained preparation in the form of gel contains hydroxyapatite, calcium triphosphate compounds and stimulators for regeneration. The proposed experimental material produces a stimulating effect on the growth of animal cell types; it enables to produce bioactive materials with increased biocompatibility. Application of the experimental gel facilitates the process of bone-tissue regeneration in the laboratory animals, which, in its turn, confirms the optimal composition of the material. We have established that during the integration of our osteoinductive material the defect zone is completely replaced by the bone tissue.

scholarly journals 3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1962 ◽  
Author(s):  
Abdullah M. Cakmak ◽  
Semra Unal ◽  
Ali Sahin ◽  
Faik N. Oktar ◽  
Mustafa Sengor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Bioactive Materials ◽  
Degradation Rates

Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.

scholarly journals COMPARATIVE EVALUATION OF BONE TISSUE REGENERATION WITH LIMITED DEFECTS OF THE PARIETAL BONE (EXPERIMENTAL STUDY ON ANIMAL MODELS)

2018 ◽  
Vol 24 (4) ◽  
pp. 180-184
Author(s):  
Natalia M. Khelminskaya ◽  
A. P Ettinger ◽  
V. I Kravets ◽  
M. D Polivoda ◽  
A. V Goncharova ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Comparative Evaluation ◽  
Calcium Sulfate ◽  
Animal Experiments ◽  
Parietal Bone ◽  
Bone Tissue Regeneration ◽  
Bone Replacement ◽  
Inflammatory Drugs ◽  
Osteoplastic Material ◽  
Mature Bone

At today, in practice of dental surgeons and maxillofacial surgeons offten used synthetic osteoplastic material which contain in its constitution hydroxyapatite,tricalcium phosphate, collagen, glycosaminoglycans (GAG), chondroitin sulfate, calcium sulfate and phosphate, antibacterial and anti-inflammatory drugs in various combinations and proportions. The bone replacement materialsserve as a framework on the basis of which in afterwards mature bone tissue forming. It should be noted that it is not possible to achieve full recovery of the lost bone tissue, in most cases unfinished regeneration is observed. In the presented work was made a comparative evaluationof the effectivness usage osteoplastic materials for replacement of limited defects of bone tissue in animal experiments.

scholarly journals ESTIMATION OF EFFICIENCY OF THE USE OF OSTEOPLASTIC MATERIAL «COLLAPAN»® IN OPERATION OF REACTION OF THE TOOTH ROOT ON THE BASIS OF COMPUTER TOMOGRAPHY

2017 ◽  
Vol 21 (5) ◽  
pp. 259-261
Author(s):  
A. Yu Rosenbaum ◽  
Valentina Petrovna Tlustenko ◽  
I. M Fedyaev
Keyword(s):  
Bone Tissue ◽  
Computer Tomography ◽  
Comparative Evaluation ◽  
Root Apex ◽  
Tooth Root ◽  
Defect Zone ◽  
Before And After ◽  
Osteoplastic Material ◽  
Methods Of Treatment ◽  
Tomography Data

Since conservative methods of treatment of chronic apical periodontitis are not always effective, the operation of resection of the apex of the tooth root does not lose its relevance. To make up for the lost bone tissue, osteoplastic materials are used, which make it possible to accelerate the regeneration process in the defect zone. These materials must be biocompatible, have osteoinductive and osteoconductive properties. With the help of computer tomography data were obtained on the structure and density of bone tissue in the area of resection of the root apex of the tooth using the material «Kollapan»® in the comparative evaluation before and after surgery.

scholarly journals Peculiarities of Bone Tissue Regeneration at Application of Osteoplastic Material in Experimental Model of Purulent Bone Wound

2014 ◽  
pp. 57-63
Author(s):  
S. N. Bugrov ◽  
V. N. Mitrofanov ◽  
D. Ya. Aleinik ◽  
K. V. Kulakova ◽  
O. P. Zhivtsov ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Defect ◽  
In Vitro Study ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Study Group ◽  
Observation Study ◽  
Study Results ◽  
Osteoplastic Material

Results of study of new osteoplastic material conditionally named «Kombas» were presented. That material consisted of nondemineralized animal collagen in a form of chips impregnated by vascular endothelium growth factor. The first step of experiment included in vitro study of the material was for cytotoxicity in diploid fibroblast cultures of 4-6 passages. At the second step purulent bone wounds were modelled in 36 Chinchilla rabbits. After debridement bone defect in the study group of animals (n=18) was filled with study material, in control group (n=18) the defect was not filled. Radiologic (X-ray, CT) and morphologic examination were performed at terms 1, 2 and 3 months. For objectification of the achieved data integral indices were proposed. Index of bone defect restoration in study group was 70% higher in 1 month, 47 % - in 2 months and 24% - in 3 months, as compared to the control group. In control group the index which characterized the completion of reparative processes exceeded that index in study group by 42% in 2 months and by 54% in 3 month of observation. Study results showed that elaborated material was not cytotoxic, possessed plasticity, marked osteoinductive and osteoconductive properties, as well as an ability to substitute bone tissue defects under conditions of purulent bone cavity in animal experiment.

scholarly journals Peculiarities of Bone Tissue Regeneration at Application of Osteoplastic Material in Experimental Model of Purulent Bone Wound

2014 ◽  
Vol 21 (2) ◽  
pp. 57-63
Author(s):  
S. N Bugrov ◽  
V. N Mitrofanov ◽  
D. Ya Aleinik ◽  
K. V Kulakova ◽  
O. P Zhivtsov ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Bone Defect ◽  
In Vitro Study ◽  
Bone Tissue Regeneration ◽  
Control Group ◽  
Study Group ◽  
Observation Study ◽  
Study Results ◽  
Osteoplastic Material

Results of study of new osteoplastic material conditionally named «Kombas» were presented. That material consisted of nondemineralized animal collagen in a form of chips impregnated by vascular endothelium growth factor. The first step of experiment included in vitro study of the material was for cytotoxicity in diploid fibroblast cultures of 4-6 passages. At the second step purulent bone wounds were modelled in 36 Chinchilla rabbits. After debridement bone defect in the study group of animals (n=18) was filled with study material, in control group (n=18) the defect was not filled. Radiologic (X-ray, CT) and morphologic examination were performed at terms 1, 2 and 3 months. For objectification of the achieved data integral indices were proposed. Index of bone defect restoration in study group was 70% higher in 1 month, 47 % - in 2 months and 24% - in 3 months, as compared to the control group. In control group the index which characterized the completion of reparative processes exceeded that index in study group by 42% in 2 months and by 54% in 3 month of observation. Study results showed that elaborated material was not cytotoxic, possessed plasticity, marked osteoinductive and osteoconductive properties, as well as an ability to substitute bone tissue defects under conditions of purulent bone cavity in animal experiment.

scholarly journals Collagen Hybrid Formulations for the 3D Printing of Nanostructured Bone Scaffolds: An Optimized Genipin-Crosslinking Strategy

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1681
Author(s):  
Giorgia Montalbano ◽  
Giorgia Borciani ◽  
Giorgia Cerqueni ◽  
Caterina Licini ◽  
Federica Banche-Niclot ◽  
...  
Keyword(s):  
3D Printing ◽  
Bone Tissue ◽  
Type I Collagen ◽  
Bone Tissue Regeneration ◽  
Type I ◽  
Bioactive Glasses ◽  
Bioactive Materials ◽  
Complex Optimization ◽  
Strontium Ions ◽  
Premature Release

Bone-tissue regeneration induced by biomimetic bioactive materials is the most promising approach alternative to the clinical ones used to treat bone loss caused by trauma or diseases such as osteoporosis. The goal is to design nanostructured bioactive constructs able to reproduce the physiological environment: By mimicking the natural features of bone tissue, the cell behavior during the regeneration process may be addressed. At present, 3D-printing technologies are the only techniques able to design complex structures avoiding constraints of final shape and porosity. However, this type of biofabrication requires complex optimization of biomaterial formulations in terms of specific rheological and mechanical properties while preserving high biocompatibility. In this work, we combined nano-sized mesoporous bioactive glasses enriched with strontium ions with type I collagen, to formulate a bioactive ink for 3D-printing technologies. Moreover, to avoid the premature release of strontium ions within the crosslinking medium and to significantly increase the material mechanical and thermal stability, we applied an optimized chemical treatment using ethanol-dissolved genipin solutions. The high biocompatibility of the hybrid system was confirmed by using MG-63 and Saos-2 osteoblast-like cell lines, further highlighting the great potential of the innovative nanocomposite for the design of bone-like scaffolds.

scholarly journals Titania Nanofiber Scaffolds with Enhanced Biointegration Activity—Preliminary In Vitro Studies

2019 ◽  
Vol 20 (22) ◽  
pp. 5642 ◽  
Author(s):  
Ehlert ◽  
Roszek ◽  
Jędrzejewski ◽  
Bartmański ◽  
Radtke
Keyword(s):  
Cell Viability ◽  
Bone Tissue ◽  
Chemical Oxidation ◽  
Bone Tissue Regeneration ◽  
High Porosity ◽  
X Ray Diffraction ◽  
Bioactive Materials ◽  
Tio2 Nanofibers ◽  
Tissue Cells

The increasing need for novel bone replacement materials has been driving numerous studies on modifying their surface to stimulate osteogenic cells expansion and to accelerate bone tissue regeneration. The goal of the presented study was to optimize the production of titania-based bioactive materials with high porosity and defined nanostructure, which supports the cell viability and growth. We have chosen to our experiments TiO2 nanofibers, produced by chemical oxidation of Ti6Al4V alloy. Fibrous nanocoatings were characterized structurally (X-ray diffraction (XRD)) and morphologically (scanning electron microscopy (SEM)). The wettability of the coatings and their mechanical properties were also evaluated. We have investigated the direct influence of the modified titanium alloy surfaces on the survival and proliferation of mesenchymal stem cells derived from adipose tissue (ADSCs). In parallel, proliferation of bone tissue cells—human osteoblasts MG-63 and connective tissue cells - mouse fibroblasts L929, as well as cell viability in co-cultures (osteoblasts/ADSCs and fibroblasts/ADSCs has been studied. The results of our experiments proved that among all tested nanofibrous coatings, the amorphous titania-based ones were the most optimal scaffolds for the integration and proliferation of ADSCs, fibroblasts, and osteoblasts. Thus, we postulated these scaffolds to have the osteopromotional potential. However, from the co-culture experiments it can be concluded that ADSCs have the ability to functionalize the initially unfavorable surface, and make it suitable for more specialized and demanding cells.

scholarly journals Adipose-derived stem cells: a review of osteogenesis differentiation

2016 ◽  
Vol 12 ◽  
pp. 38-47 ◽  
Author(s):  
Aleksandra Skubis ◽  
Bartosz Sikora ◽  
Nikola Zmarzły ◽  
Emilia Wojdas ◽  
Urszula Mazurek
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Regenerative Medicine ◽  
Bone Tissue ◽  
Tissue Regeneration ◽  
Cell Types ◽  
Bone Tissue Regeneration ◽  
Adipose Derived Stem Cells

This review article provides an overview on adipose-derived stem cells (ADSCs) for implications in bone tissue regeneration. Firstly this article focuses on mesenchymal stem cells (MSCs) which are object of interest in regenerative medicine. Stem cells have unlimited potential for self-renewal and develop into various cell types. They are used for many therapies such as bone tissue regeneration. Adipose tissue is one of the main sources of mesenchymal stem cells (MSCs). Regenerative medicine intends to differentiate ADSC along specific lineage pathways to effect repair of damaged or failing organs. For further clinical applications it is necessary to understand mechanisms involved in ADSCs proliferation and differentiation. Second part of manuscript based on osteogenesis differentiation of stem cells. Bones are highly regenerative organs but there are still many problems with therapy of large bone defects. Sometimes there is necessary to make a replacement or expansion new bone tissue. Stem cells might be a good solution for this especially ADSCs which manage differentiate into osteoblast in in vitro and in vivo conditions.

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