scholarly journals Titania Nanofiber Scaffolds with Enhanced Biointegration Activity—Preliminary In Vitro Studies

2019 ◽  
Vol 20 (22) ◽  
pp. 5642 ◽  
Author(s):  
Ehlert ◽  
Roszek ◽  
Jędrzejewski ◽  
Bartmański ◽  
Radtke
Keyword(s):  
Cell Viability ◽  
Bone Tissue ◽  
Chemical Oxidation ◽  
Bone Tissue Regeneration ◽  
High Porosity ◽  
X Ray Diffraction ◽  
Bioactive Materials ◽  
Tio2 Nanofibers ◽  
Tissue Cells

The increasing need for novel bone replacement materials has been driving numerous studies on modifying their surface to stimulate osteogenic cells expansion and to accelerate bone tissue regeneration. The goal of the presented study was to optimize the production of titania-based bioactive materials with high porosity and defined nanostructure, which supports the cell viability and growth. We have chosen to our experiments TiO2 nanofibers, produced by chemical oxidation of Ti6Al4V alloy. Fibrous nanocoatings were characterized structurally (X-ray diffraction (XRD)) and morphologically (scanning electron microscopy (SEM)). The wettability of the coatings and their mechanical properties were also evaluated. We have investigated the direct influence of the modified titanium alloy surfaces on the survival and proliferation of mesenchymal stem cells derived from adipose tissue (ADSCs). In parallel, proliferation of bone tissue cells—human osteoblasts MG-63 and connective tissue cells - mouse fibroblasts L929, as well as cell viability in co-cultures (osteoblasts/ADSCs and fibroblasts/ADSCs has been studied. The results of our experiments proved that among all tested nanofibrous coatings, the amorphous titania-based ones were the most optimal scaffolds for the integration and proliferation of ADSCs, fibroblasts, and osteoblasts. Thus, we postulated these scaffolds to have the osteopromotional potential. However, from the co-culture experiments it can be concluded that ADSCs have the ability to functionalize the initially unfavorable surface, and make it suitable for more specialized and demanding cells.

Chitosan-alginate Hybrid Scaffolds for in Vitro Bone Tissue Regeneration

2005 ◽  
Vol 873 ◽  
Author(s):  
Zhensheng Li ◽  
Hassna R. Ramay ◽  
Kip D. Hauch ◽  
Miqin Zhang
Keyword(s):  
Bone Tissue ◽  
Porous Scaffold ◽  
Mechanical Test ◽  
Biological Properties ◽  
Bone Tissue Regeneration ◽  
High Porosity ◽  
Thermally Induced ◽  
Mineral Deposition ◽  
Alginate Scaffold

AbstractThis paper reports the development of a biodegradable porous scaffold made from naturally derived chitosan and alginate polymers for bone tissue engineering. The scaffold has a 3-D interconnected porous structure and was fabricated through thermally induced phase separation. The mechanical test showed that the scaffold has compressive strength of 0.46 ± 0.02 MPa — about 4 times that of the pure chitosan scaffold. The cell-material interaction study indicated that osteoblast cells seeded on the chitosan-alginate scaffold attach and proliferate well. The mineral deposition occurred after 3 days of culture and formed large chunks after 7 days. The chitosan-alginate scaffold was also found to have desirable swelling property and was structurally stable in solutions of different pH. The chitosan-alginate scaffold can be prepared from solutions of neutral pH allowing growth factors to be incorporated homogeneously into the scaffold for sustained release. This research demonstrated a technique by which a polymer-based biodegradable scaffold can be made to have high porosity up to 92% and excellent mechanical and biological properties.

Thermal and in vitro evaluation of a composite material pHEMA/Chitosan/Hydroxyapatite

2015 ◽  
Vol 1767 ◽  
pp. 133-138
Author(s):  
Areli.M. Salgado-Delgado ◽  
Zully Vargas-Galarza ◽  
René Salgado-Delgado ◽  
Efraín Rubio-Rosas ◽  
Edgar García-Hernández ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Regeneration ◽  
Body Fluid ◽  
Bone Tissue Regeneration ◽  
Gravimetric Analysis ◽  
Hydroxyethyl Methacrylate ◽  
Thermal Gravimetric ◽  
In Vitro Bioactivity ◽  
Bioactive Materials

ABSTRACTBioactive materials based on polymer/hydroxyapatite are currently being extensively investigated as materials for promotion of bone tissue regeneration and reconstruction [1]. In this work, a material interpenetrating based on poly 2-hydroxyethyl methacrylate (pHEMA), Chitosan and hydroxyapatite (HA) was prepared following the methodology of the foaming gas Damla Çetin [2], generating an interpenetrated network with the chitosan filled with hydroxyapatite. The materials were evaluated by thermal gravimetric analysis (TGA) and in vitro bioactivity [3] (SBF) and characterized by using scanning electron microscopy (SEM). The TGA studies suggested that there was not existence of possible interactions between polymers and HA but there is a thermal stability increase in the HA content. Meanwhile, SBF and its characterization by SEM, was found that the materials are bioactives as indicated by the formation of a bone-like apatite layer after immersion in simulated body fluid, indicating the potential of this material for use in bone tissue engineering.

scholarly journals Dual Network Composites of Poly(vinyl alcohol)-Calcium Metaphosphate/Alginate with Osteogenic Ions for Bone Tissue Engineering in Oral and Maxillofacial Surgery

2021 ◽  
Vol 8 (8) ◽  
pp. 107
Author(s):  
Lilis Iskandar ◽  
Lucy DiSilvio ◽  
Jonathan Acheson ◽  
Sanjukta Deb
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Vinyl Alcohol ◽  
Maxillofacial Surgery ◽  
Bone Defects ◽  
Bone Tissue Regeneration ◽  
Poly Vinyl Alcohol ◽  
Oral And Maxillofacial Surgery

Despite considerable advances in biomaterials-based bone tissue engineering technologies, autografts remain the gold standard for rehabilitating critical-sized bone defects in the oral and maxillofacial (OMF) region. A majority of advanced synthetic bone substitutes (SBS’s) have not transcended the pre-clinical stage due to inferior clinical performance and translational barriers, which include low scalability, high cost, regulatory restrictions, limited advanced facilities and human resources. The aim of this study is to develop clinically viable alternatives to address the challenges of bone tissue regeneration in the OMF region by developing ‘dual network composites’ (DNC’s) of calcium metaphosphate (CMP)—poly(vinyl alcohol) (PVA)/alginate with osteogenic ions: calcium, zinc and strontium. To fabricate DNC’s, single network composites of PVA/CMP with 10% (w/v) gelatine particles as porogen were developed using two freeze–thawing cycles and subsequently interpenetrated by guluronate-dominant sodium alginate and chelated with calcium, zinc or strontium ions. Physicochemical, compressive, water uptake, thermal, morphological and in vitro biological properties of DNC’s were characterised. The results demonstrated elastic 3D porous scaffolds resembling a ‘spongy bone’ with fluid absorbing capacity, easily sculptable to fit anatomically complex bone defects, biocompatible and osteoconductive in vitro, thus yielding potentially clinically viable for SBS alternatives in OMF surgery.

scholarly journals Osteogenic Properties of 3D-Printed Silica-Carbon-Calcite Composite Scaffolds: Novel Approach for Personalized Bone Tissue Regeneration

2021 ◽  
Vol 22 (2) ◽  
pp. 475
Author(s):  
Parastoo Memarian ◽  
Francesco Sartor ◽  
Enrico Bernardo ◽  
Hamada Elsayed ◽  
Batur Ercan ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Bone Tissue ◽  
Cell Attachment ◽  
Three Dimensional ◽  
Bone Tissue Regeneration ◽  
Hemolysis Assay ◽  
Novel Approach ◽  
Related Transcription Factor ◽  
Diphenyl Tetrazolium Bromide

Carbon enriched bioceramic (C-Bio) scaffolds have recently shown exceptional results in terms of their biological and mechanical properties. The present study aims at assessing the ability of the C-Bio scaffolds to affect the commitment of canine adipose-derived mesenchymal stem cells (cAD-MSCs) and investigating the influence of carbon on cell proliferation and osteogenic differentiation of cAD-MSCs in vitro. The commitment of cAD-MSCs to an osteoblastic phenotype has been evaluated by expression of several osteogenic markers using real-time PCR. Biocompatibility analyses through 3-(4,5-dimethyl- thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), lactate dehydrogenase (LDH) activity, hemolysis assay, and Ames test demonstrated excellent biocompatibility of both materials. A significant increase in the extracellular alkaline phosphatase (ALP) activity and expression of runt-related transcription factor (RUNX), ALP, osterix (OSX), and receptor activator of nuclear factor kappa-Β ligand (RANKL) genes was observed in C-Bio scaffolds compared to those without carbon (Bio). Scanning electron microscopy (SEM) demonstrated excellent cell attachment on both material surfaces; however, the cellular layer on C-Bio fibers exhibited an apparent secretome activity. Based on our findings, graphene can improve cell adhesion, growth, and osteogenic differentiation of cAD-MSCs in vitro. This study proposed carbon as an additive for a novel three-dimensional (3D)-printable biocompatible scaffold which could become the key structural material for bone tissue reconstruction.

scholarly journals 3D Printed Polycaprolactone/Gelatin/Bacterial Cellulose/Hydroxyapatite Composite Scaffold for Bone Tissue Engineering

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 1962 ◽  
Author(s):  
Abdullah M. Cakmak ◽  
Semra Unal ◽  
Ali Sahin ◽  
Faik N. Oktar ◽  
Mustafa Sengor ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bacterial Cellulose ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Bone Tissue Regeneration ◽  
Engineering Applications ◽  
Bioactive Materials ◽  
Degradation Rates

Three-dimensional (3D) printing application is a promising method for bone tissue engineering. For enhanced bone tissue regeneration, it is essential to have printable composite materials with appealing properties such as construct porous, mechanical strength, thermal properties, controlled degradation rates, and the presence of bioactive materials. In this study, polycaprolactone (PCL), gelatin (GEL), bacterial cellulose (BC), and different hydroxyapatite (HA) concentrations were used to fabricate a novel PCL/GEL/BC/HA composite scaffold using 3D printing method for bone tissue engineering applications. Pore structure, mechanical, thermal, and chemical analyses were evaluated. 3D scaffolds with an ideal pore size (~300 µm) for use in bone tissue engineering were generated. The addition of both bacterial cellulose (BC) and hydroxyapatite (HA) into PCL/GEL scaffold increased cell proliferation and attachment. PCL/GEL/BC/HA composite scaffolds provide a potential for bone tissue engineering applications.

Bioactive and Biocompatible Silica/Pseudowollastonite Aerogels

2014 ◽  
Vol 96 ◽  
pp. 21-26 ◽  
Author(s):  
P.J. Reséndiz-Hernández ◽  
D.A. Cortés-Hernández ◽  
Juan Méndez Nonell ◽  
J.C. Escobedo-Bocardo
Keyword(s):  
Bone Tissue ◽  
Ambient Pressure ◽  
Sol Gel ◽  
Ambient Pressure Drying ◽  
Bone Tissue Regeneration ◽  
Tissue Formation ◽  
Potential Applications ◽  
Ft Ir ◽  
Bone Tissue Formation

Silica aerogels have attracted increasingly more attention due to their extraordinary properties and their existing and potential applications in a wide variety of technological areas. Materials that promote bone-tissue formation at their surface and bond to osseous tissues when implanted are called bioactive, such as pseudowollastonite particles. In this work, the synthesis of aerogels with pseudowollastonite particles was performed. The synthesis involved the preparation of an alcogel by a two step sol-gel route followed by ambient pressure drying. To promote a higher bioactivity the obtained aerogels were then biomimetically treated using simulated body fluids, SBF and 1.5 SBF. A high bioactivity was demonstrated by FT-IR, SEM, EDS, and XRD. The in vitro biocompatibility was assessed by testing cytotoxicity using rat osteoblasts cultures. The results obtained indicate that these materials are highly potential aerogels for bone tissue regeneration.

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