scholarly journals Молекулярно-генетическая изменчивость генов 18S-rRNA и 28S-rRNA у цестод рода Bothriocephalus Rud., 1808 (Cestoda: Bothriocephalidea) из рыб Чёрного моря

2020 ◽  
Author(s):  
Yu.V. Slynko ◽  
T.A. Polyakova ◽  
E.E. Slynko
Keyword(s):  
Black Sea ◽  
18S Rrna ◽  
Molecular Genetic Analysis ◽  
The Body ◽  
The Black Sea ◽  
Rrna Gene ◽  
28S Rrna ◽  
Crimean Peninsula ◽  
Nucleotide Variability ◽  
Scorpaena Porcus

Проведён молекулярно-генетический анализ фрагментов двух генов 18S-rRNA (длиной 568 п.н.) и 28S-rRNA (длиной 312 п.н.) цестод рода Bothriocephalus Rud., 1808, паразитирующих у скорпены Scorpaena porcus (Linnaeus, 1758) и у черноморской камбалы Scophthalmus maeoticus (Pallas, 1814), обитающих в Чёрном море. Материал был собран в северной части Чёрного моря возле побережья Крымского полуострова. Пробы тела паразитов фиксировали в 96 этаноле. В результате, как по каждому гену в отдельности, так и при их объединении установлено, что образец, извлечённый из скорпены (деп. в NCBI MH011407 18S-rRNA и MH000375 28S-rRNA), надёжно идентифицируется, как относящийся к кладе, содержащей B. timii, B. scorpii и B. australis, р-расстояние между нашим образцом и другими видами этой группы не превышает 1,6. Остальные три образца MH011408, MH011409, MH011410 (для гена 18s-rRNA) и MH000376 (для гена 28s-rRNA) сформировали отдельную кладу, состоящую из двух субклад: одна включает образцы МН011409 и МН011410, другая образец МН011408 (указаны только регистрационные номера для гена 18s-rRNA). Следует также отметить, что виды Bothriocephalus timii и Bothriocephalus scorpii дистанцированы всего лишь на 0,5, а Bothriocephalus timii и Bothriocephalus australis на 0,6. Гаплотипы вида Bothriocephalus claviceps составили внешнюю группу, р-расстояние от которого всех, как наших образцов, так и рассматриваемых видов комплекса scorpio , не опускалось ниже 26,3. Вместе с тем, дистанцированность объединённых гаплотипов цестод из черноморской камбалы на уровне 45 р-расстояний, а также значения бутстрепа позволяют полагать их близнецовыми видами (или подвидами) в пределах рода, по аналогии с генами мтДНК. В результате анализа нуклеотидной изменчивости данных фрагментов генов подтверждена принадлежность рассматриваемых экземпляров к роду Bothriocephalus, и они идентифицированы как виды, входящие в комплекс видов Bothriocephalus scorpii .Molecular genetic analysis of fragments of the two genes 18S-rRNA (568 bps long) and 28S-rRNA (312 bps long) cestodes of the genus Bothriocephalus Rud., 1808, parasitizing in Scorpaena porcus (Linnaeus, 1758) and at the Black Sea flounder Scophthalmus maeoticus (Pallas, 1814) living in the Black Sea. Material was collected in the northern Black Sea off the coast of the Crimean Peninsula. Samples of the body of parasites were fixed in 96 ethanol. As a result it was established both for each gene individually and when combining them that the sample extracted from scorpion ( in NCBI MH011407 18S-rRNA and MH000375 28S-rRNA) is reliably identified as referring to clade containing B. timii, B. scorpii and B. australis, the p-distance between our sample and other species of this group does not exceed 1.6. The remaining three samples MH011408, MH011409, MH011410 (for the 18s-rRNA gene) and MH000376 (for the 28s-rRNA gene) formed a separate clade which consists of two subclades: one includes samples MH011409 and MH011410 the other sample MH011408 (only registration numbers are indicated for MH011408 gene 18s-rRNA). It should also be noted that the species Bothriocephalus timii and Bothriocephalus scorpii are only 0.5 apart while Bothriocephalus timii and Bothriocephalus australis are 0.6 apart. Haplotypes of the species Bothriocephalus claviceps constituted an external group, the p-distance from which of all both our samples and the species of the scorpio complex under consideration did not fall below 26.3. At the same time the distance between the combined haplotypes of cestodes from the Black Sea flounder at the level of 45 p-distances as well as bootstrap values allow us to consider them to be twin species (or subspecies) within the genus by analogy with mtDNA genes. An analysis of the nucleotide variability of these gene fragments confirmed the affiliation of the examined species to the genus Bothriocephalus and they were identified as species forming part of the complex of species Bothriocephalus scorpii.

A new dagger nematode, Xiphinema poasense n. sp. (Nematoda: Longidoridae), from Costa Rica

Nematology ◽  
2018 ◽  
Vol 20 (3) ◽  
pp. 235-252 ◽  
Author(s):  
Ingrid Varela-Benavides ◽  
Walter Peraza-Padilla ◽  
Carolina Cantalapiedra-Navarrete ◽  
Juan E. Palomares-Rius ◽  
Pablo Castillo ◽  
...  
Keyword(s):  
Costa Rica ◽  
18S Rrna ◽  
Mitochondrial Gene ◽  
The Body ◽  
Wild Plants ◽  
Rrna Gene ◽  
28S Rrna ◽  
Body Contour ◽  
Genital Branch ◽  
Partial 18S

A new dagger nematode,Xiphinema poasensen. sp., is described and illustrated from three populations extracted from soil associated with a combined plantation ofEucalyptussp.,Cupressussp. andPennisetumsp. and wild plants from a tropical pre-montane forest in Costa Rica. The new dagger nematode is characterised by a moderate body size 2612 (2416-3042) μm long, a rounded lip region 15.0 (13.5-16.5) μm broad, separated from the body contour by a shallow depression, amphidial fovea large, stirrup-shaped, a very long odontostyle (175 (164-188) μm), stylet guiding ring located 167 (136-181) μm from anterior end, vulva situated anterior to mid-body (36-40%), anterior genital branch complete but strongly reduced, without uterine differentiation, female tail short, hemispherical to convex-conoid with a c′ ratio = 0.7 (0.6-0.8) and bearing two pairs of caudal pores, and male absent. Integrative diagnosis was completed with molecular data using D2-D3 expansion segments of 28S rRNA, ITS1 region, partial 18S-rRNA and the partial mitochondrial gene cytochrome c oxidase subunit 1 (coxI). The phylogenetic relationships based on D2-D3 segments of this species with otherXiphinemaspp. of theX.non-americanumgroup indicated thatX. poasensen. sp. clustered with other species with a reduced anterior genital branch from the morphospecies Group 2,viz.,X. costaricenseandX. krugi. However, the phylogeny ofcoxIand partial 18S rRNA gene revealed that the new species did not cluster withXiphinemaspecies having the anterior genital branch absent or reduced (i.e., morphospecies Groups 1 and 2, respectively).

scholarly journals Sex Differences and Sex Identification in the Small-Scaled Scorpionfish, Scorpaena Porcus (Scorpaenidae, Scorpaeniformes)

2016 ◽  
Vol 50 (4) ◽  
pp. 355-362
Author(s):  
V. N. Peskov ◽  
L. G. Manilo
Keyword(s):  
Sex Differences ◽  
Black Sea ◽  
Coastal Waters ◽  
Morphological Characters ◽  
Sex Identification ◽  
The Body ◽  
The Black Sea ◽  
Southern Coast ◽  
The Absolute ◽  
Scorpaena Porcus

Abstract Sex differences and the ability to determine the sex of Scorpaena porcus (Linnaeus, 1758) on morphological characters were studied in the population inhabiting coastal waters of the Black Sea near the southern coast of Crimea. These differences were revealed in size (females are larger than males) and in proportions of the body. It is found that variation of the absolute and relative values in plastic characters is higher in females compared with males. It is shown that in 92 % of individuals belonging to S. porcus we can determine the sex using morphological characters with a probability of 99.9-100 %.

scholarly journals Crassicauda boopis in a fin whale (Balaenoptera physalus) ship-struck in the eastern North Atlantic Ocean

2017 ◽  
Vol 3 ◽  
Author(s):  
LAETITIA LEMPEREUR ◽  
MORGAN DELOBELLE ◽  
MARJAN DOOM ◽  
JAN HAELTERS ◽  
ETIENNE LEVY ◽  
...  
Keyword(s):  
North Atlantic ◽  
18S Rrna ◽  
North Atlantic Ocean ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
Balaenoptera Physalus ◽  
Fin Whale ◽  
Eastern North Atlantic ◽  
The Right

SUMMARY On 9 November 2015, a juvenile male fin whale of 11·60 m length was observed on the bulb of a merchant vessel in the Channel Terneuzen – Ghent (The Netherlands – Belgium). A severe parasitosis was present in the right heart ventricle and caudal caval vein. Parasites were identified as Crassicauda boopis based on macroscopic and microscopic observations. The sequence of the 18S rRNA gene obtained from the parasite samples was 100% similar to the sequence of the 18S rRNA gene from Crassicauda magna available on GenBank. While adults of C. boopis and C. magna are morphologically distinct and found at different locations in the body, the molecular analysis of the 18S rRNA gene seems insufficient for reliable species identification. Although numerous C. boopis were found, the cause of death was identified as due to the collision with the ship, as suggested by the presence of a large haematoma, and the absence of evidence of renal failure. The young age of this whale and the absence of severe chronic reaction may suggest that the infestation was not yet at an advanced chronic stage.

Steinernema borjomiense n. sp. (Rhabditida: Steinernematidae), a new entomopathogenic nematode from Georgia

Nematology ◽  
2018 ◽  
Vol 20 (7) ◽  
pp. 653-669 ◽  
Author(s):  
Oleg Gorgadze ◽  
Elena Fanelli ◽  
Manana Lortkhipanidze ◽  
Alberto Troccoli ◽  
Medea Burjanadze ◽  
...  
Keyword(s):  
New Species ◽  
18S Rrna ◽  
Entomopathogenic Nematode ◽  
Phylogenetic Analyses ◽  
Tail Length ◽  
Morphological Characters ◽  
18S Rrna Gene ◽  
The Body ◽  
Rrna Gene ◽  
A New Species

Summary A new species of entomopathogenic nematode, Steinernema borjomiense n. sp., was isolated from the body of the host insect, Oryctes nasicornis (Coleoptera: Scarabaeidae), in Georgia, in the territory of Borjomi-Kharagauli. Morphological characters indicate that the new species is closely related to species of the feltiae-group. The infective juveniles are characterised by the following morphological characters: body length of 879 (777-989) μm, distance between the head and excretory pore = 72 (62-80) μm, pharynx length = 132 (122-142) μm, tail length = 70 (60-80) μm, ratio a = 26.3 (23.0-29.3), H% = 45 (40-51), D% = 54 (47-59), E% = 102 (95-115), and lateral fields consisting of seven ridges (eight incisures) at mid-body. Steinernema borjomiense n. sp. was molecularly characterised by sequencing three ribosomal regions (the ITS, the D2-D3 expansion domains and the 18S rRNA gene) and the mitochondrial COI gene. Phylogenetic analyses revealed that S. borjomiense n. sp. differs from all other known species of Steinernema and is a member of the monticolum-group.

Analysis of potential seismic sources of tsunamis in the Black Sea region, using data from various catalogues

2021 ◽  
Author(s):  
Emil Oynakov ◽  
Liliya Dimitrova ◽  
Lyubka Pashova ◽  
Dragomir Dragomirov
Keyword(s):  
Black Sea ◽  
Scientific Evidence ◽  
Active Faults ◽  
Active Regions ◽  
The Black Sea ◽  
Earthquake Catalogues ◽  
Crimean Peninsula ◽  
Black Sea Region ◽  
Global Science ◽  
Using Data

<p>Low-laying territories along the Black Sea coastal line are more vulnerable to the possible high (long) waves due to tsunami events caused by strong earthquakes in the active seismic regions. Historically, such events are rare in the Black Sea region, despite some scientific evidence of tsunamis and their recordings through continuous sea-level observations with tide gauges built in certain places along the coast. This study analyses seismic data derived from different international earthquake catalogues - NEIC, ISC, EMSC, IDC and Bulgarian national catalogue (1981 - 2019). A catalogue of earthquakes within the period covering the historical to the contemporary seismicity with magnitudes M ≥ 3 is compiled. The data are processed applying the software package ZMAP, developed by Stefan Wiemer (http://www.seismo.ethz.ch/en/research-and-teaching/products-software/software/ZMAP/index.html). The catalogues' completeness is calculated to assess the reliability of the historical data needed to assess the risk of rare tsunami events. The prevailing part of the earthquakes' epicentres are in the seismically active regions of Shabla, the Crimean peninsula, the east and southeast coast of the Black Sea forming six main clusters, which confirmed previous studies in the region. In these areas, several active and potentially active faults, which can generate tsunamigenic seismic events, are recognized.</p><p><strong>Acknowledgements: </strong>The authors would like to thank the Bulgarian National Science Fund for co-funding the research under the Contract КП-СЕ-КОСТ/8, 25.09.2020, which is carried out within framework of COST Action 18109 “Accelerating Global science In Tsunami HAzard and Risk analysis” (AGITHAR; https://www.agithar.uni-hamburg.de/).</p>

scholarly journals Salinity optima for vegetative growth and sexual reproduction of the diatom Toxarium undulatum

2020 ◽  
Vol 5 (1) ◽  
pp. 20-28
Author(s):  
N. A. Davidovich ◽  
O. I. Davidovich
Keyword(s):  
Sexual Reproduction ◽  
Black Sea ◽  
Salinity Tolerance ◽  
Vegetative Growth ◽  
Sea Water ◽  
The Black Sea ◽  
Crimean Peninsula ◽  
Wide Range ◽  
Clonal Cultures ◽  
Oceanic Species

Distribution of diatom algae is limited by their tolerance to environmental factors. Although a genus Toxarium has been evolving for more than 100 million years, it is represented by only two species. Toxarium undulatum is widely spread in tropical and subtropical seas, and it can be also found in the Black Sea, the salinity of which is twice lower than the oceanic one. Ecological and psychological characteristics research of this species is of great interest in terms of its relationship to salinity. T. undulatum clonal cultures were sampled in the Donuzlav Lake connected to the Black Sea (southwest of the Crimean Peninsula) and on Gran Canaria coast (Canary Islands archipelago). Experiments on the salinity tolerance limits showed, that the Black Sea clones were viable in a range of at least 30 ‰ (12 to 42 ‰). The same wide range of salinity tolerance with slightly higher values was observed among oceanic clones of this species. Optima of vegetative growth and sexual reproduction were determined. Optima of the Black Sea clones appeared to be 27.8 and 27.2 ‰, respectively, which was significantly higher than salinity observed in population habitat. Similar higher optima of vegetative growth and sexual reproduction, compared with those salinity values, at which natural population developed, were observed for a number of other Black Sea diatoms, which proved their oceanic (Mediterranean) origin. It was concluded that T. undulatum, along with other species, began to populate the Black Sea basin about seven thousand years ago after Mediterranean Sea water started to flow into the freshened Novoevksinsky Sea-Lake through the Bosporus Strait. However, the evolution rate did not allow bringing physiological and ecological characteristics of the species studied into full agreement with environmental conditions. Oceanic origin is evidently seen in its physiological reactions to salinity. Possibility of speciation due to settlement of the Black Sea with oceanic species is discussed.

scholarly journals First Report of Ditylenchus dipsaci on Garlic in Minnesota

Plant Disease ◽  
2012 ◽  
Vol 96 (11) ◽  
pp. 1707-1707 ◽  
Author(s):  
D. S. Mollov ◽  
S. A. Subbotin ◽  
Carl Rosen
Keyword(s):  
The Body ◽  
Parasitic Nematodes ◽  
Rrna Gene ◽  
28S Rrna ◽  
Gene Sequences ◽  
Surrounding Water ◽  
Pharyngeal Bulb ◽  
First Report ◽  
Plant Parts ◽  
Ditylenchus Dipsaci

In the summer of 2011, two independent garlic samples from Morrison and Dakota counties and in 2012 one garlic sample from Carver county in Minnesota were submitted by commercial growers to the University of Minnesota Plant Disease Clinic for disease analyses. Symptoms of the above-ground plant parts were stunting and chlorosis. Symptoms of bulbs were necrosis, underdevelopment, and distortion. Upon microscopic examination, phytonematodes exuded into the surrounding water droplet. Nematodes were present in the protective leaves, abscission zone, and cloves in all submitted bulbs (n = 18) for analyses. Morphometric examination of females, males, and juveniles determined that they were Ditylenchus dipsaci. Nematodes extracted from garlic cloves were fixed in TAF (97 ml formalin [40%], 2 ml triethanolamine, and 91 ml dH2O). Morphological observations and measurements were made under an Olympus BX51 microscope equipped with a Nomarski differential interference contrast. Female (n = 6) measurements were: L = 1.411 to 1.636 mm, a = 38 to 44, b = 5.8 to 8.0, c = 14 to 17, stylet = 11.5 to 12.3 μm, V = 79 to 81%, and tail = 95 to 105 μm. The body was almost straight, when heat relaxed, lip region flattened, median bulb oval, and isthmus elongate and slender. The basal pharyngeal bulb overlapped the intestine. The post-vulval uterine branch was about half of vulva-anus distance. The tail was conoid with a pointed terminus. Male (n = 9) measurements were: L = 1.372 to 1.558 mm, a = 40 to 50, b = 6.5 to 7.0, c = 14 to 16, stylet = 11.5 to 12.3 μm, spicules = 22 to 27 μm, and gubernaculum = 9 to 10 μm. The bursa was leptoderan and spicules were curved with simple gubernaculum. Morphology and morphometrics of females and males of D. dipsaci from Minnesota generally fit the descriptions provided for the type and other populations by Hopper (1) and other authors. Several specimens were also taken for molecular identification. DNA extraction, PCR, and sequencing protocols were as described by Subbotin et al. (2). The TW81 and AB28 primers were used for amplification of ITS-rRNA region and the D2A and D3B primers were used for amplification of the D2-D3 expansion segments of 28S rRNA gene. Comparison of the ITS and D2-D3 of 28 rRNA gene sequences showed 100 and 99% identity with corresponding gene sequences of D. dipsaci published in the GenBank (2). The sequences were submitted in the GenBank under accession numbers JX123258 and X123259. This nematode problem has not been known to occur in either of these locations previously. The most likely source of introduction of D. dipsaci are imported garlic seed bulbs. To our knowledge, this is the first report of D. dipsaci affecting garlic or any other crops in Minnesota. The garlic produced in these locations was considered unmarketable and complete loss to the farmers. The presence of D. dipsaci could have a significant economic impact in the emerging multi-million dollar garlic industry in Minnesota. References: (1) D. J. Hooper. Ditylenchus dipsaci. CIH Descriptions of Plant-Parasitic Nematodes Set 1, No. 14, 1972. (2) S. A. Subbotin et al. Phytopathology 95:1308, 2005.

Nephtyidae (Annelida: Polychaeta) from the Sea of Marmara and Black Sea, with descriptions of two new species

Zootaxa ◽  
2021 ◽  
Vol 5060 (2) ◽  
pp. 33-64
Author(s):  
SEVGI KUŞ ◽  
GÜLEY KURT ◽  
MELIH ERTAN ÇINAR
Keyword(s):  
New Species ◽  
Body Size ◽  
Black Sea ◽  
The Body ◽  
The Black Sea ◽  
Sea Of Marmara ◽  
Marine Fauna ◽  
Black Sea Coast ◽  
Two New Species ◽  
Sea Coast

The present paper deals with the diversity of nephtyid polychaetes (Nephtyidae) from the Sea of Marmara and the Black Sea. Three species belonging to two genera (Micronephthys and Nephtys) were found in the Black Sea (coast of Turkey) and six species belonging to three genera (Inermonephtys, Micronephthys and Nephtys) were found in the Sea of Marmara. The material includes two species new to science, Inermonephtys turcica n. sp. and Nephtys sinopensis n. sp., and a species record (Nephtys kersivalensis McIntosh, 1908) new to the Sea of Marmara’s marine fauna. Nephtys sinopensis n. sp. is mainly characterized by having 1–4 geniculate chaetae in the postacicular position of the parapodia; digitiform antennae, palps, and ventral cirri at chaetiger 1 with swollen tips; small and cirriform branchiae present from chaetiger 4 to the end of the body; poorly developed parapodial prechaetal lamellae in median and posterior chaetigers and long ventral cirri along the body. Inermonephtys turcica n. sp. is mainly characterized by having cushion-like palps with digitiform tips; well developed neuropodial postchaetal lamellae; barred chaetae in preacicular position of the anterior and median parapodia; and branchiae first appearing between chaetiger 3 and 13 (depending on body size).  

A new species of Cymbasoma (Copepoda, Monstrilloida) from the northern coast of Turkey (Black Sea) with comments on the C. longispinosum species-group

Crustaceana ◽  
2014 ◽  
Vol 87 (11-12) ◽  
pp. 1393-1410 ◽  
Author(s):  
F. Üstün ◽  
T. Terbiyik Kurt ◽  
E. Suárez-Morales
Keyword(s):  
New Species ◽  
Black Sea ◽  
Gulf Of California ◽  
Relative Length ◽  
Species Group ◽  
The Body ◽  
The Black Sea ◽  
Northern Coast ◽  
Black Sea Coast ◽  
The One

Cymbasoma sinopense sp. nov. is described from an adult female collected off the Sinop coast, in the southern Black Sea, Turkey. This is the first species of this genus recorded from the Black Sea coast of Turkey. The new species is a member of the widespread Cymbasoma longispinosum species-group, and it closely resembles C. chelemense Suárez-Morales & Escamilla, 1997 from the Yucatan Peninsula and C. californiense Suárez-Morales & Palomares-García, 1999 from the Gulf of California. This species can be distinguished by a combination of subtle characters, including the body proportions, the structure and armature of the fifth legs, the ornamentation of the genital somite, and the relative length of the ovigerous spines. A previous report of C. longispinosum from the northern Black Sea probably represented a misidentified record of C. sinopense sp. nov. The Mediterranean reports refer to a species that is different from the one of the Black Sea. Comparative comments and data including the main taxonomic characters of members of the Cymbasoma longispinosum species-group are presented. Overall, it is confirmed that records of this species from different geographic areas should be revised carefully because they could represent undescribed species. A key for the identification of the females of the currently known species assigned to the Cymbasoma longispinosum species-group is included.

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