scholarly journals Uji daya hambat ekstrak etanol daun srikaya (Annona squamosa) terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Alberta Y.M. Tansil ◽  
Edward Nangoy ◽  
Jimmy Posangi ◽  
Robert A. Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Annona Squamosa ◽  
Diffusion Technique ◽  
Experimental Laboratory ◽  
Inhibition Zones ◽  
The Mean

Abstract: Srikaya leaf contains terpenoid, polyphenol, alkaloid, and flavonoid that can potentially be an antibacterial. This study was aimed to obtain the potency of srikaya leaf extract against Staphylococcus aureus (ATCC25923) and Escherichia coli (ATCC11229). This was an experimental laboratory study using the Kirby-Bauer modified well diffusion technique in the Phytochemistry and Microbiology Laboratory of MIPA Faculty at Sam Ratulangi University. Srikaya leaf extract was obtained by using ethanol maceration technique. The concentrations of the extract were as follows: 50%, 25%, and 12.5%. Ciprofloxacin was used as the positive control while CMC as the negative one. The results showed that CMC did not have any inhibition zone around the well. Ciprofloxacin showed the largest mean diameters of inhibition zones: 35.78 mm against E.coli and 36.55 mm against S.aureus. The mean diameters of inhibition zones of Srikaya leaf extract 50% were 9.13 mm against E.coli and 13.78 mm against S.aureus. The mean diameters of inhibition zones of Srikaya leaf extract 25% were 7.8 mm against E.coli and 13.55 mm agaisnt S.aureus. Meanwhile, the mean diameters of inhibition zones of srikaya leaf extract 12.5% were 7.05 mm against E.coli and 11.31mm agaimst S.aureus. Conclusion: Srikaya leaf extract could potentially inhibit the growth of S.aureus and E.coli. The srikaya leaf extract could inhibit S.aureus more effectively than E.coli.Keyword: antibacterial, srikaya leaf extract, Staphylococcus aureus, Escherichia coli  Abstrak: Daun Srikaya mengandung terpenoid, fenolik, alkaloid, dan flavonoid yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui uji daya hambat ekstrak daun srikaya terhadap Staphylococcus aureus (ATCC25923) dan Escherichia coli (ATCC11229). Jenis penelitian ialah eksperimental laboratorium dengan modifikasi Kirby-Bauer sumuran di Laboratorium Fitokimia dan Mikrobiologi Fakultas MIPA Universitas Sam Ratulangi. Ekstrak daun srikaya diperoleh dari proses maserasi dengan etanol 96%. Konsentrasi ekstrak kental yang digunakan ialah 50%, 25%, 12,5%. Siprofloksasin digunakan sebagai kontrol positif dan CMC sebagai kontrol negatif. Hasil penelitian mendapatkan CMC tidak mempunyai zona hambat. Siprofloksasin memiliki diameter zona hambat yang paling besar. Rerata diameter zona hambat yang dihasilkan oleh siprofloksasin ialah 35,78 mm terhadap bakteri E.coli dan 36,55 mm terhadap S.aureus. Rerata diameter zona hambat ekstrak daun srikaya 50% ialah 9,13 mm terhadap E.coli dan 13,78 mm terhadap bakteri S.aureus. Rerata diameter zona hambat ekstrak daun srikaya 25% ialah 7,8 mm terhadap E.coli dan 13,25 mm terhadap S.aureus. Rerata diameter zona hambat ekstrak daun srikaya 12,5% ialah 7,05 mm terhadap E.coli dan 11,31 mm terhadap S.aureus. Simpulan: Ekstrak daun srikaya berpotensi memiliki efek daya hambat terhadap pertumbuhan bakteri S.aureus dan E.coli. Daya hambat ekstrak daun srikaya lebih besar terhadap S.aureus daripada E.coli.Kata kunci: antibakteri, ekstrak daun srikaya, Staphylococcus aureus, Escherichia coli

scholarly journals Uji Efek Antibakteri Chromodoris dianae terhadap Bakteri Escherichia coli dan Staphylococcus aureus

2019 ◽  
Vol 7 (1) ◽  
Author(s):  
Eflentina Kipimbob ◽  
Robert Bara ◽  
Pemsi M. Wowor ◽  
Jimmy Posangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Experimental Study ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Antibacterial Effects ◽  
E Coli ◽  
Mean Diameter ◽  
The Mean

Abstract: This study was aimed to evaluate the antibacterial effect of Chromodoris dianae on E.coli and S. aureus. This was an experimental study. Samples of Chromodoris dianae was taken from Bunaken waters by diving. Extract of Chromodoris dianae was obtained by using maceration technique with 96% etanol. Antibacterial activity of this extract was tested by using the Kirby-Bauer method. The results showed that the mean diameter of the inhibition zone of E. coli was 22.3±1.5 mm and of S. aureus was 23.0±1.0 mm; both were were less than of ciprofloxacin as the positive control repeated for three times. Conclusionn: Chromodoris dianae has antibacterial effects on the growth of Escherichia coli and Staphylococcus aureus.Keywords: Chromodoris dianae, Staphylococcus aureus, Escherichia coli Abstrak: Penelitian ini bertujuan untuk melihat adanya efek antibakteri dari Chromodoris dianae terhadap bakteri E.coli dan S. aureus. Jenis penelitian ialah eksperimental. Sampel Chromodoris dianae diambil dari perairan Bunaken dengan cara menyelam. Ekstrak Chromodoris dianae dibuat dengan cara maserasi menggunakan etanol 96%. Pengujian aktivitas antibakteri dilakukan dengan menggunakan metode Kirby-Bauer. Hasil penelitian menunjukkan adanya zona hambat terhadap E. coli dan S. aureus dengan rerata diameter 22,3±1,5 mm dan 23,0±1,0 mm, yang lebih kecil daripada rerata diameter kontrol positif siprofloksasin pada tiga kali pengulangan. Simpulan: Chromodoris dianae memiliki efek antibakteri terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Kata kunci: Chromodoris dianae, Staphylococcus aureus, Escherichia coli

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Sukun (Artocarpus altilis) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli

2020 ◽  
pp. 10-20
Author(s):  
Fuan Maharani Fiana ◽  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Ery Purwanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Artocarpus Altilis ◽  
Escherichia Coli Bacteria

Diarrhea is caused by bacterial infections, especially Staphylococcus aureus and Escherichia coli. The breadfruit leaves (Artocarpus altilis) are known contain flavonoid compounds, alkaloids, saponins, and tannins which can be used as antibacterial. This research was conducted to determine the ethanol extract of the breadfruit leaves against Staphylococcus aureus and Escherichia coli bacteria. The manufacture of the breadfruit leaf extract was using a maceration method with 96% ethanol solvent. The result of the tube test and the TLC test was positive, the breadfruit leaf extract containing flavonoids and tannins which have potential as antibacterial. The antibacterial test was carried out using the disc diffusion method (Kirby-Bauer test) against Staphylococcus aureus and Escherichia coli bacteria incubated for 1 x 24 hours. The extract concentrations tested were 10%, 15% and 20%. The positive control was using erythromycin antibiotics and the negative control was using aquades. The identification of the chemical content of plants is done by tube test and the TLC test. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Staphylococcus aureus bacteria, the average diameter of inhibitory zones respectively were 3.67, 3.50 and 2.67 mm with the positive control diameter of inhibition zone was 18.5 mm, the negative control diameter of inhibition zone was 0 mm. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Escherichia coli bacteria, the average diameter of inhibitory zones respectively were 5.33, 3.17 and 3.33 mm with the positive control inhibition zone diameter of 28.5 mm and the negative control of inhibition zone diameter of 0 mm. The activity of ethanol extract of the breadfruit leaves against the Staphylococcus aureus and Escherichia coli bacteria is included in the weak category.

scholarly journals Uji daya hambat ekstrak daun patikan kerbau (euphorbia hirta l.) terhadap pertumbuhan bakteri staphylococcus aureus dan escherichia coli

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Florencia I. Mahmud ◽  
Christi Mambo ◽  
Henoch Awaloei
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Inhibitory Effect ◽  
Positive Control ◽  
Negative Control ◽  
Euphorbia Hirta ◽  
E Coli ◽  
Extract Concentration

Abstract: Patikan kerbau leaf contains alkaloid, flavonoid, phenol, and tannin can potentially be an antibacterial. The purpose of this research is to test the resisting potency of patikan kerbau leaf extract against Staphylococcus aureus (ATCC25923) dan Escherichia coli (ATCC11229). This was an experimental laboratory study using modified Kirby-Bauer with well diffusion technique at Research and Microbiology Laboratory of MIPA Faculty, Sam Ratulangi University Manado. Patikan kerbau leaf extract was obtained by using 96% etanol maceration. Extract concentrations used in this study were 50 mg/ml, 25 mg/ml, 12.5 mg/ml and 6.25 mg/ml. Ciprofloxacin was used as the positive control and CMC as the negative control. The CMC showed no inhibition zone. Ciprofloxacin had the widest zone of inhibition. The average of inhibition zone diameters produced by ciprofloxacin was 33,3 mm on S. aureus and 33 mm on E.coli. Euphorbia hirta leaf extract concentration of 50 mg/ml resulted in average inhibition zone diameter of 18.83 mm on S.aureus and 17.83 mm on E.coli. Extract concentration of 25 mg/ml resulted in 17.33 mm on S. aureus and 16.83 mm on E.coli. Extract concentration of 12,5 mg/ml resulted in 15.5 mm on S. aureus and 14.83 mm on E.coli. Then, extract concentration of 6.25 mg/ml resulted in 15.16 mm on S. aureus and 13.3 mm on E.coli. Conclusion: Extract of Euphorbia hirta leaf has potential inhibitory effect towards bacterial growth of S. aureus and E. coli. Moreover, the inhibitory effect of Euphorbia hirta extract is greater towards S. aureus rather than E.coliKeywords: antibacterial, patikan kerbau leaf extract, S. aureus, E. coli Abstrak: Daun patikan kerbau mengandung alkaloid, flavonoid, fenol dan tanin yang berpotensi sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui uji daya hambat ekstrak daun patikan kerbau terhadap Staphylococcus aureus (ATCC25923) dan Escherichia coli (ATCC11229). Jenis penelitian ini ialah eksperimental laboratorium di Laboratorium Penelitian dan Mikrobiologi Fakultas MIPA Universitas Sam Ratulangi Manado dengan metode Kirby-Bauer yang dimodifikasi dengan sumuran. Ekstrak daun patikan kerbau diperoleh dari proses maserasi dengan etanol 96%. Konsentrasi ekstrak yang digunakan dalam penelitian ialah 50mg/ml, 25mg/ml, 12,5mg/ml dan 6,25mg/ml. Siprofloksasin digunakan sebagai kontrol positif dan CMC sebagai kontrol negatif. Pada penelitian ini CMC yang tidak mempunyai zona hambat. Siprofloksasin memiliki diameter zona hambat yang paling besar. Rerata diameter zona hambat yang dihasilkan oleh siprofloksasin adalah 33,3 mm terhadap bakteri S.aureus dan 33 mm pada bakteri E.coli. Ekstrak daun patikan kerbau konsentrasi 50 mg/ml menghasilkan diameter zona hambat rata-rata sebesar 18,83 mm pada bakteri S.aureus dan 17,83 mm pada bakteri E.coli. Ekstrak daun patikan kerbau konsentrasi 25mg/ml sebesar 17,3 mm pada bakteri S.aureus dan 16,83 mm pada bakteri E.coli. Ekstrak daun patikan kerbau konsentrasi 12,5mg/ml sebesar 15,5 mm pada bakteri S.aureus dan 14,83 mm pada bakteri E.coli. Kemudian, konsentrasi 6,25mg/ml sebesar 15,16 pada bakteri S.aureus dan 13,3 mm pada bakteri E.coli. Simpulan: Ekstrak daun patikan kerbau berpotensi memiliki efek daya hambat terhadap pertumbuhan bakteri S.aureus dan E.coli. Daya hambat ekstrak daun patikan kerbau lebih besar pada S.aureus daripada E.coli Kata kunci: antibakteri, ekstrak daun srikaya, S. aureus, E. coli

scholarly journals Uji daya hambat ekstrak daun lidah mertua (Sansevieriae trifasciata folium) terhadap pertumbuhan bakteri Escherichia coli dan Streptococcus sp

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Brily Lombogia ◽  
Fona Budiarso ◽  
Widdhi Bodhi
Keyword(s):  
Escherichia Coli ◽  
Laboratory Study ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Antibacterial Effects ◽  
E Coli ◽  
Experimental Laboratory ◽  
Inhibition Zones

Abstract: Mother in law’s tongue plant has some active compounds inter alia saponin, polyphenol, and flavonoid that have antibacterial effects. This study aimed to identify whether the antibacterial effects of mother in law’s tongue leaf (Sansevieria Trifasciata) towards the growth of Escherichia coli and Streptococcus sp. This was an experimental laboratory study. The concentrations of mother in law’s tongue leaf extract were tested with well methods, as follows: 5%, 10%, 20%, and 40%. The results showed that this extract at concentration of 5%, 10%, 20%, and 40% could inhibit the growth of E. coli with the average diameters of inhibition zones as follows: 7.8 mm, 13 mm, 14.5 mm, and 17.3 mm meanwhile of Streptococcus sp. with the average diameters of inhibition zones, as follows: 4.6 mm, 9.6 mm, 13 mm, and 15.3 mm. Conclusion: Ethanol extract of mother in law’s tongue leaves (Sansevieria Trifasciata) has antibacterial activities against the growth of E. coli and Streptococcus sp. The higher the concentration is, the broader the inhibition zone is.Keywords: Sansevieriae trifasciata folium, inhibition zone, E. coli, Streptococcus sp. Abstrak: Tanaman Lidah Mertua (Sansevieria Trifasciata) memiliki senyawa aktif yaitu Saponin, Polifenol, dan Flavonoid yang mampu bekerja sebagai antibakteri. Penelitian ini bertujuan untuk mengetahui ada tidaknya daya hambat ekstrak daun lidah mertua (Sansevieria trifasciata) terhadap pertumbuhan bakteri E. coli, dan Streptococcus sp. Jenis penelitian ini eksperimental laboratorik. Kadar ekstrak etanol daun lidah mertua (Sansevieria trifasciata) yang diujikan dengan metode sumuran yaitu 5%, 10%, 20%, dan 40%. Ekstrak etanol daun lidah mertua (Sansevieria trifasciata) dengan konsentrasi 5%, 10%, 20%, dan 40% dapat menghambat pertumbuhan bakteri E. coli dengan rerata diameter zona hambat masing-masing yaitu 7,8 mm, 13 mm, 14,5 mm, dan 17,3 mm sedangkan Streptococcus sp. dengan masing-masing rerata diameter zona hambat yaitu 4,6 mm, 9,6 mm, 13 mm, dan 15,3 mm. Simpulan: Ekstrak etanol daun lidah mertua (Sansevieria trifasciata) mempunyai aktifitas antimikroba terhadap pertumbuhan bakteri E. coli dan Streptococcus sp, dimana makin tinggi konsentrasi ekstrak daun lidah mertua, makin luas zona jernih pada media kultur bakteri E. coli dan Streptococcus sp. Kata kunci: Sansevieriae trifasciata folium, daya hambat, E. coli, Streptococcus sp.

scholarly journals Uji daya hambat jamur endofit rimpang lengkuas (Alpinia galanga l.) terhadap pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Vita A.D Putri ◽  
Jimmy Posangi ◽  
Edward Nangoy ◽  
Robert A. Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Endophytic Fungi ◽  
Inhibition Zone ◽  
Alpinia Galanga ◽  
Average Value ◽  
Diffusion Technique ◽  
Rich Media ◽  
Experimental Laboratory ◽  
Alternative Antibiotic

Abstract: Endophytic fungi could be used as an alternative antibiotic because it produced bioactive compounds, which are developed as basic ingredients for medicine such as antibiotic, antioxcide, anticancer. Endophytic fungi can be isolated from Rhizome of Alpinia galanga L. which is abundant in Indonesia. The aim of this study was to find the inhibiting zone of endophytic fungi rhizome of Alpinia galanga L. to the growth of Staphylococcus aueus and Escherichia coli. This was an experimental laboratory study using modified Kirby-Bauer well diffusion technique in Marine Pharmaceutical and Biology Molecular laboratory of FPIK faculty in Sam Ratulangi University. Endophytic fungi were produced by Rhizome of Alpinia galanga L. which are cultured in carbohydrate-rich media. Then, it produced various fungi isolate, which is being used for testing its bioactiy to Staphylococcus aureus and Escherichia coli. The results showed that only 2 isolates of endophytic fungi that have inhibitory and the range of the average value of inhibition zone endophytic fungi rhizome of Alpinia galanga L. against Staphylococcus aureus 19 mm to 21.3 mm, while the Escherichia coli 21.3 mm to 22.3 mm. Conclusion: Endophytic fungi Rhizome of Alpinia galanga L. could inhibit the bacterial growth of Staphylococcus aureus and Escherichia coli.Keywords: Staphylococcus aureus, Escherichia coli, endophytic fungi Abstrak: Jamur endofit salah satu sumber bahan baku obat yang memproduksi senyawa bioaktif potensial dalam menghasilkan efek antibiotik, antikanker, antioksidan. Jamur endofit dapat ditemukan pada tanaman Lengkuas Alpinia galanga L. yang banyak terdapat di Indonesia. Penelitian ini bertujuan untuk mengetahui daya hambat jamur endofit terhadap Staphylococcus aureus dan Escherichia coli. Jenis penelitian ialah eksperimental laboratorik dengan modifikasi Kirby-Bauer sumuran di Laboratorium Biologi Molekular dan Farmasetika Laut FPIK-UNSRAT. Jamur endofit yang dihasilkan dari rimpang Lengkuas Alpinia galanga L. yang dikultur dalam media kaya karbohidrat menghasilkan berbagai isolat jamur yang kemudian diuji bioaktivitasnya terhadap Staphylococcus aureus dan Escherichia coli. Hasil penelitian mendapatkan hanya 2 isolat jamur endofit yang memiliki daya hambat dan kisaran nilai rata-rata zona hambat jamur endofit rimpang Lengkuas Alpinia galanga L. terhadap Staphylococcus aureus 19 mm sampai dengan 21,3 mm, sedangkan terhadap Escherichia coli 21,3 mm sampai dengan 22,3 mm. Simpulan: Jamur endofit rimpang lengkuas Alpinia galanga L. dapat menghambat pertumbuhan Staphylococcus aureus dan Escherichia coli. Daya hambat jamur endofit lebih besar pada Escherichia coli daripada Staphylococcus. Kata kunci: Staphylococcus aureus, Escherichia coli, jamur endofit

scholarly journals Uji Efek Antibakteri Chromodoris annae terhadap Bakteri Staphylococcus aureus dan Escherichia coli

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Aqueline N. Faidiban ◽  
Jimmy Posangi ◽  
Pemsi M. Wowor ◽  
Robert A. Bara
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Effect ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
The Mean ◽  
Escherichia Coli Bacteria

Abstract: This study was aimed to evaluate the antibacterial effect of Chromodoris annae taken from Bunaken waters. This was an experimental study. The antibacterial effect was tested by using the Kirby-Bauer method. Chromodoris annae extract was made by maceration using 95% ethanol and was tested to Staphylococcus aureus and Escherichia coli bacteria. Ciprofloxacin was used as the positive control and aquadest as the negative control. The results showed that the mean inhibition zone diameter of Chromodoris annae extract to Staphylococcus aureus was 22.3 mm meanwhile of ciprofloxacin was 34.7 mm. The mean inhibition zone diameter of Chromodoris annae extract to Escherichia coli was 23.0 mm meanwhile of ciprofloxacin was 40.3 mm. Moreover, aquadest showed no inhibition zone. In conclusion, Chromodoris annae had very strong antibacterial effect to the growths of Staphylococcus aureus and Escherichia coli.Keywords: Chromodoris annae, Staphlococcus aureus, Escherichia coli Abstrak: Penelitian ini bertujuan untuk mengevaluasi efek antibakteri dari Chromodoris annae yang diambil dari perairan Bunaken. Jenis penelitian ialah eksperimental. Pengujian aktivitas antibakteri menggunakan metode Kirby-Bauer. Ekstrak Chromodoris annae dibuat dengan cara maserasi menggunakan pelarut etanol 95% dan diujikan aktivitas antibakteri terhadap bakteri Staphylococcus aureus dan Escherichia coli. Sebagai kontrol positif digunakan antibiotik ciprofloxacin dan sebagai kontrol negatif digunakan akuades. Hasil penelitian memperlihatkan rerata zona hambat ekstrak Chromodoris annae terhadap Staphylococcus aureus sebesar 22,3 mm sedangkan zona hambat ciprofloxacin sebesar 34,7 mm. Rerata zona hambat ekstrak Chromodoris annae terhadap Escherichia coli sebesar 23,0 mm sedangkan zona hambat ciprofloxacin sebesar 40,3 mm. Akuades tidak memperlihatkan adanya zona hambat. Simpulan penelitian ini ialah Chromodoris annae memiliki efek antibakteri kategori sangat kuat terhadap pertumbuhan bakteri Staphylococcus aureus dan Escherichia coli.Kata kunci: Chromodoris annae, Staphylococcus aureus, Escherichia coli

scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

Antibacterial Activity of Amine-Functionalized Zeolite NaY against Staphylococcus aureus ATCC6538 and Escherichia coli ATCC11229

2015 ◽  
Vol 761 ◽  
pp. 402-406 ◽  
Author(s):  
Siti Aishah Mohd Hanim ◽  
Nik Ahmad Nizam Nik Malek ◽  
Zaharah Ibrahim ◽  
Mashitah Mad Salim ◽  
Nur Isti'anah Ramli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gram Positive ◽  
Gram Negative ◽  
Zeolite Nay ◽  
Amine Functionalized ◽  
Diffusion Technique

The antibacterial activity of functionalized zeolite NaY (CBV100) with different concentrations of 3-aminopropyltriethoxysilane (APTES) (0.01, 0.05, 0.20 and 0.40 M) was studied against Staphylococcus aureus ATCC 6538 (Gram positive) and Escherichia coli ATCC 11229 (Gram negative) through disc diffusion technique (DDT). The characterization of functionalized zeolite NaY with fourier transform infrared (FTIR) spectroscopy indicated the attachment of APTES on zeolite NaY. Through DDT, the inhibition zone of functionalized zeolite NaY increased proportionally to the amount of the amine-functional group attached onto zeolite NaY. Functionalized zeolite NaY showed higher antibacterial activity against Gram-positive compared to Gram-negative bacteria. It can be concluded from this study that amine-functionalized zeolite NaY shows evidence of antibacterial activities.

Note: Effect of Some Spice Extracts on Bacterial Inhibition

2003 ◽  
Vol 9 (5) ◽  
pp. 353-358 ◽  
Author(s):  
O. Sagdic ◽  
A. G. Karahan ◽  
M. Ozcan ◽  
G. Ozkan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Yersinia Enterocolitica ◽  
Inhibition Zone ◽  
Bacterial Strains ◽  
Inhibitory Effects ◽  
Antibacterial Effects ◽  
E Coli ◽  
Inhibition Zones ◽  
Mrs Broth

Eighteen extracts of spices commonly consumed worldwide and grown naturally in Turkey were tested against twenty three bacterial strains to compare their antibacterial effects with eleven antibiotics. Eight pathogens and fifteen lactobacilli isolated from chick intestine were used as the test microorganisms. Pathogens (six different Staphylococcus aureus strains, Escherichia coli ATCC 25922 and Yersinia enterocolitica ATCC 1501) were grown in Nutrient broth and lactobacilli in MRS broth. Hop extracts formed inhibition zones against S. aureus strains of upto 36 mm. Inhibitory effects of hop extracts against S. aureuswere generally higher than that of erythromycin as antibiotic. Helichrysum compactum extract produced an inhibition zone of 23mm to E. coli ATCC 25922 and 26mm to Y. enterocolitica ATCC 1501. Helichrysum compactum extract inhibited the growth of Y. enterocolitica ATCC 1501 more than other spice extracts. While inhibition zones of these extracts against lactobacilli were found smaller than on S. aureus strains, inhibition zones of the same extracts against lactobacilli were found similar to those of E. coli ATCC 25922 and Y. enterocolitica ATCC 1501.

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