scholarly journals UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 19-23
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

UJI AKTIVITAS ANTIBAKTERI DARI EKSTRAK ETANOL DAUN PERIA LAUT (Colubrina Asiatica L.) TERHADAP BAKTERI STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI

2019 ◽  
Vol 2 (1) ◽  
pp. 14-18
Author(s):  
Firdaus Fahdi ◽  
Harwitavia Harwitavia ◽  
Herviani Sari
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Antibiotic Drugs ◽  
Escherichia Coli Bacteria

The discovery of new antibiotic drugs is getting more and more reactive. The plant of the peria laut leaf is one of the drugs that is often used as a traditional medicine and contains bioactive compounds of polyphenols, flavonoids, and saponins, which can inhibit antibacterial growth. Purpose of this study was to determine the antibacterial activity of ethanol extract of peria laut leaves (Colubrina asiatica L.) against Staphylococcus aureus and Escherichia coli bacteria. Method the experimental, of the sample used was concentrated marine peria laut leaf extract of 25mg/ml, 50mg/ml, 75mg/ml, 100mg/ml, 200mg/ml, 300mg/ml, 400mg/ml, and 500mg/ml, positive control of amoxicillin tablet 500 mg, negative control of dimethylsulfoxide with the method of disc diffusion testing using media Nutrient Agar. Results the showed thet the peria laut leaf extract positively contained bioactive alkaloid compounds, flavonoids, saponins, steroids, and tannins, and had inhibitory effects on Staphylococcus aureus and Escgerichia coli bacteria with various concentrations which had been tested on the average area of the highest inhibition zone of 18,6 mm in Staphylococcus aureus bacteria, and 10,2 mm in Escherichia coli bacteria. Conclusion peria laut leaf extract (Colubrina asiatica L.) has antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria in the most inhibitory zone at a concentration of 500mg/ml with a diameter of 18,6 in Staphylococcus aureus bacteria and 10,2 in Escherichia coli.

scholarly journals Aktivitas Antibakteri Ekstrak Etanol Daun Sukun (Artocarpus altilis) Terhadap Bakteri Staphylococcus aureus Dan Escherichia coli

2020 ◽  
pp. 10-20
Author(s):  
Fuan Maharani Fiana ◽  
Naelaz Zukhruf Wakhidatul Kiromah ◽  
Ery Purwanti
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Leaf Extract ◽  
Ethanol Extract ◽  
Average Diameter ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Artocarpus Altilis ◽  
Escherichia Coli Bacteria

Diarrhea is caused by bacterial infections, especially Staphylococcus aureus and Escherichia coli. The breadfruit leaves (Artocarpus altilis) are known contain flavonoid compounds, alkaloids, saponins, and tannins which can be used as antibacterial. This research was conducted to determine the ethanol extract of the breadfruit leaves against Staphylococcus aureus and Escherichia coli bacteria. The manufacture of the breadfruit leaf extract was using a maceration method with 96% ethanol solvent. The result of the tube test and the TLC test was positive, the breadfruit leaf extract containing flavonoids and tannins which have potential as antibacterial. The antibacterial test was carried out using the disc diffusion method (Kirby-Bauer test) against Staphylococcus aureus and Escherichia coli bacteria incubated for 1 x 24 hours. The extract concentrations tested were 10%, 15% and 20%. The positive control was using erythromycin antibiotics and the negative control was using aquades. The identification of the chemical content of plants is done by tube test and the TLC test. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Staphylococcus aureus bacteria, the average diameter of inhibitory zones respectively were 3.67, 3.50 and 2.67 mm with the positive control diameter of inhibition zone was 18.5 mm, the negative control diameter of inhibition zone was 0 mm. The results of incubation of the breadfruit leaf extract with concentrations of 10%, 15% and 20% against Escherichia coli bacteria, the average diameter of inhibitory zones respectively were 5.33, 3.17 and 3.33 mm with the positive control inhibition zone diameter of 28.5 mm and the negative control of inhibition zone diameter of 0 mm. The activity of ethanol extract of the breadfruit leaves against the Staphylococcus aureus and Escherichia coli bacteria is included in the weak category.

scholarly journals Antibacterial Activity Test of the C-4-methoxyphenylcalix[4]resorcinarene Compound Modified by Hexadecyltrimethylammonium-Bromide against Staphylococcus aureus and Escherichia coli Bacteria

2018 ◽  
Vol 3 (3) ◽  
pp. 201 ◽  
Author(s):  
Suryadi Budi Utomo ◽  
Mita Fujiyanti ◽  
Warih Puji Lestari ◽  
Sri Mulyani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Hexadecyltrimethylammonium Bromide ◽  
E Coli ◽  
Escherichia Coli Bacteria

<p>This study aims to determine the antibacterial activity of C-4-methoxyphenylcalix-[4]resorcinarene modified by Hexadecyltrimethylammonium-Bromide (Resorcinarene-HDTMA-Br) against <em>Staphylococcus aureus</em> and <em>Escherichia coli</em> bacteria. The study was conducted by testing the inhibition zone diameter with paper disc diffusion method. Variations in the concentration of the sample used were 10%; 15%; 20%; 25%; and 30% b/v. In this study, Dimethyl sulfoxide (DMSO) (sampel solvent) was used as a negative control and Chloramphenicol as a positive control. It was found that Resorsinarene-HDTMA-Br had higher antibacterial activity than the unmodified C-4-methoxyphenylcalix[4]resorcinarene (CMFKR) against the <em>S.</em><em> </em><em>aureus</em> bacteria. Both CMFKR and Resorcinarene-HDTMA-Br are not active against <em>E. coli</em>.</p>

UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOL DAUN MEKAI (Pycnarrhena cauliflora DIELS.) TERHADAP Staphylococcus aureus

2020 ◽  
Vol 6 (2) ◽  
pp. 246
Author(s):  
Yayuk Bulam Sarifati ◽  
Sjarif Ismail ◽  
Khemasili Kosala
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antimicrobial Agents ◽  
Statistical Tests ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Control Measurement ◽  
Eye Pain

Mekai leaves (Pycnarrhena cauliflora Diels.) (P. cauliflora). Are known to contain flavonoid compounds, tannins and phenolics that act as antibacterial agents and are used in the treatment of eye pain. Staphylococcus aureus (S. aureus) is one of the examples of bacterial diseases of eye pain and also a major cause of many infections in communities and health facilities with cases of resistance to various antimicrobial agents. The purpose of this study was to prove the antibacterial activity of mekai leaves ethanol extract against S. aureus bacteria. This research is an experimental research. The stages of this research began by extracting mekai leaves using maceration method with 96% ethanol solvent. Antibacterial activity was tested by the disc method (Kirby-Bauer) using ethanol extract concentrations of mekai leaves (EPC) 20%, 30%, 40%, 50%, 60%, 70%, and 80%, positive control using 25 μg amoxicillin and negative control using DMSO 10%. The measurement results of inhibition zones of mekai leaf ethanol extract 20%, 30%, 40%, 50%, 60%, 70% and 80% respectively were 8.32 mm, 8.32 mm, 8.32 mm, 8.67 mm, 9.00 mm, 8.67 mm, and 8.33 mm. While the positive control measurement of 25 μg amoxicillin against S. aureus is 28.67 mm and the measurement of 10% negative DMSO control does not produce inhibitory zones, so it can be concluded that the ethanol extract of mekai leaves has antibacterial activity, but the area of ​​inhibition zone produced is smaller than amoxicillin 25 µg. The results of statistical tests using Mann Whitney between negative controls with all EPC concentrations obtained significant differences with p values ​​<0.05, it can be concluded that there is antibacterial activity produced at all EPC concentrations.

scholarly journals The Effect of Various Concentrations of Ethanol Extract of the Leaves of Paederia foetida L. on the Growth of Escherichia Coli Bacteria

2021 ◽  
Vol 11 (6) ◽  
pp. 61-67
Author(s):  
Hertina Silaban
Keyword(s):  
Escherichia Coli ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Positive Control ◽  
Negative Control ◽  
Rare Plants ◽  
E Coli ◽  
Paederia Foetida ◽  
Escherichia Coli Bacteria ◽  
The One

Bacterial infection of Escherichia coli (E. coli) as the cause of gastrointestinal disorders in humans has increased their prevalence. Treatment using natural ingredients can be a choice of therapy because of the minimal side effects. One of the rare plants believed by the community as an antibacterial is stinking vin’e known as the ‘leaf fart’. The purpose of this research is for knowing the activity of the ethanol extract of Paederia foetida L can affect the growth of E.coli. The serial diffusion disc method is being used as the antibacterial activity test. The concentration  of this extract are 10%, 20%, 40%, 80%, 100% with positive control (ciprofloxacin) and negative control (aqua dest). The inhibition zone diameter characterized the effect of Extract on bacterial growth were 6.16 mm of the concentration 10%, 6.667 mm of the concentration 20%, 7.10 mm of the concentration 40 %, 7.78 mm of the concentration 80%, and 10.03 mm of the concentration 100%. As for the negative control has no effect. The study stated that the higher concentration of antibacterial agent used, the greater the inhibition zone formed. Based on the result of the analysis of the data by using the One-Way ANOVA Test showed a probability value (p) = 0.000 or value (p) < 0.05, that H0 is rejected and H1 is accepted. The conclusion is that the Extract of stinking vin’e has an antibacterial effect on the growth of E.coli. Keywords: Antibacterial, E.coli, Extract of  Sembukan leaf

scholarly journals AKTIVITAS ANTIBAKTERI EKSTRAK DAN FRAKSI KARANG LUNAK Sinularia SP. DI TELUK MANADO

PHARMACON ◽  
2019 ◽  
Vol 8 (1) ◽  
pp. 114
Author(s):  
Lyone Katiandagho ◽  
Defny S Wewengkang ◽  
Sri Sudewi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Soft Coral ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Chloroform Fraction ◽  
Water Fraction

ABSTRACT Soft Coral Sinularia sp.has been shown to have antibacterial activity. This research aims to determine the antibacterial activity of marine  organisms obtained from Manado bay. The method in this study is the diffusion method with positive control chloramphenicol and negative control of methanol in Escerichia coli and Sthaphylococcus aureus. The result showed that extracts, chloroform fraction and methanol-water fraction were obtained inhibiting category of Sthapylococcus aureus bacteria, but in the chloroform fraction and ethanol extract can inhibit Escerichia coli bacteria categorized as strong. With it can be conlueded that the extract and fraction of soft Sinularia sp. have bioactive compounds with a broth spectrum of antibacterial activity.Keywords: Sinularia sp., Chloramphenicol, Escerichia coli, Sthaphylococcus aureus, and antibacterial. ABSTRAK Karang lunak Sinularia sp. telah terbukti memiliki aktivitas antibakteri. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri pada organisme biota laut yang diperoleh dari teluk Manado. Metode dalam penelitian ini adalah metode difusi agar dengan kontrol positif kloramfenikol dan kontrol negatit metanol pada Escherichia coli dan Staphylococcus aureus. Hasil penelitian menunjukkan bahwa ekstrak, fraksi kloroform, dan fraksi metanol-air dapat menghambat bakteri Staphylococcus aereus dikategorikan sedang, namun pada fraksi kloroform dan ekstrak etanol dapat menghambat bakteri Escerichia coli dikategorikan kuat. Dengan demikian dapat disimpulkan bahwa ekstrak dan fraksi karang lunak Sinularia sp.memiliki senyawa bioaktif dengan spektrum yang luas terhadap aktivitas antibakteri.Kata kunci: Sinularia sp, Kloramfenikol, Escherichia coli, Staphylococcus aureus, dan antibakteri

scholarly journals UJI AKTIVITAS ANTIBAKTERI DAUN JERUK KALAMANSI (Citrofortunella microcarpa) TERHADAP BAKTERI Staphylococcus aureus DAN Escherichia coli

Alotrop ◽  
2019 ◽  
Vol 3 (2) ◽  
Author(s):  
Deza Oktasila ◽  
Nurhamidah Nurhamidah ◽  
Dewi Handayani
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Essential Oil ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Raw Material ◽  
Diffusion Method ◽  
Citrus Leaves ◽  
Escherichia Coli Bacteria

This study aims to examine the antibacterial activity of ethanol extract and essential oil of Kalamansi citrus leaves (Citrofortunella microcarpa) against Staphylococcus aureus and Escherichia coli bacteria. The raw material of Kalamansi citrus leaves is obtained from the Village of Pondok Kubang, Bengkulu Tengah (3.70060S, 102.35780E). Ethanol extract from Kalamansi citrus leaves was obtained by maceration using ethanol 96%, then ethanol extract was made dilution concentration 40 ; 20; , 10; and 5%. The essential oil of Kalamansi citrus leaves is obtained by water-vapor distillation, then made variations of concentration 20;, 15;, 10;  and 5%. The method used to test the antibacterial activity is the paper disc diffusion method , the antibacterial activity is shown by the diameter of the inhibiting  zone formed. The data of antibacterial test result were analyzed by using One Way Anova test which showed the effect of treatment on the growth of test bacteria seen from the value (P <0,01) and continued by Duncan test to know the effect of the treatment. The results showed that ethanol extract of Kalamansi citrus leaves had antibacterial activity against S. aureus and E. coli bacteria with moderate inhibitory diameter is  7.20 and 5.73 mm at concentration 40%, while antibacterial activity on essential oil of Kalamansi citrus leaves is categorized as strong with inhibition zone diameter is 14.83 and 13.00 mm at concentration 20%.

scholarly journals Fitokimia Dan Aktivitas Antibakteri Dari Daun KAF(Chisocheton sp. (C. DC) HARMS)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 42
Author(s):  
Florentin Natalia Melsadalam ◽  
Dewa Gede Katja ◽  
Meiske S. Sangi
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Leaf Extract ◽  
Inhibition Zone ◽  
Diffusion Method ◽  
Inhibitory Zone ◽  
Escherichia Coli Bacteria

Telah dilakukan penelitian yang bertujuan untuk mengidentifikasi senyawa metabolit sekunder  dari ekstrak daun kaf menggunakan tiga jenis pelarut yakni n-heksan, etil asetat, dan metanol. Ekstrak yang diperoleh diuji aktivitas antibakterinya dengan metode difusi yang menggunakan bakteri Staphylococcusaureus dan Escherichia coli. Dari hasil pengujian fitokimia, ketiga ekstrak tersebut menunjukan bahwa sampel mengandung senyawa; alkaloid, saponin, steroid, flavonoid dan tanin. Pengujian aktivitas antibakteri  dengan metode difusi menunjukan hasil yang paling tinggi aktivitas antibakteri adalah  estrak etil asetat dengan zona hambat 35,75 mm terhadap bakteri  Staphylococcus aureus, sedangkan pada ekstrak metanol memiliki zona hambat 23,75 mm terhadap bakteri Escherichia coliResearch has been carried out aimed at identifying secondary metabolites of kaf leaf extract using three types of solvents, namely n-hexane, ethyl acetate, and methanol.The obtained extracts were tested for antibacterial activity by diffusion method using Staphylococcus aureus and Escherichia coli bacteria. From the results of phytochemical testing, the three extracts showed that the sample contained compounds; alkaloids, saponins, steroids, flavonoids and tannins. Antibacterial activity testing with diffusion method showed the highest antibacterial activity was estracethyl ethyl acetate with 35.75 mm inhibition zone against Staphylococcus aureus bacteria, whereas methanol had 23.75 mm inhibitory zone against Escherichia coli bacteria

scholarly journals UJI DAYA HAMBAT EKSTRAK ETANOL DAUN KACAPIRING (Gardenia augusta) TERHADAP BAKTERI Staphylococcus aureus, Escherichia coli DAN Salmonella thypi

PHARMACON ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 268
Author(s):  
Serly D. S. Toding ◽  
Herny E. I. Simbala ◽  
Deby A. Mpila
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Medicinal Plants ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diffusion Method ◽  
Inhibitory Power ◽  
Paper Disc

ABSTRACT One of the potential medicinal plants as traditional medicine is gardenia plant (Gardenia augusta). Gardenia leaf extract contains alkaloid compounds, flavonoids, tannins, saponins and triterpenoids, which are known to have antibacterial abilities..The aim of this research was to determine the antibacterial activity of gardenia leaf extract on the growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi. The examination on the inhibitory power using the Kirby-Bauer method. The Kirby-Bauer method is referring to the diffusion method using paper disc with five treatments, namely the extract of gardenia leaves with a substance concentration of 20%, 40%, and 60% as well positive control (Ciprofloxacin) and negative control (aquades). Based on the research results obtained, it can be concluded that gardenia leaf extract can inhibit growth of Staphylococcus aureus, Escherichia coli and Salmonella thypi at a concentration of 20%, 40% and 60% and classified as strong and medium category. Keywords  :    Gardenia augusta, antibacterial, of Staphylococcus aureus, Escherichia coli, Salmonella thypiABSTRAK  Salah satu tanaman obat dengan potensi sebagai obat tradisional adalah tanaman kacapiring (Garddenia augusta). Daun kacapiring mengandung senyawa alkaloid, flavonoid, tanin, saponin dan triterpenoid, yang berpotensi sebagai antibakteri sehingga digunakan dalam penelitian ini.Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak daun kacapiring terhadap pertumbuhan Staphylococcus aureus, Escherichia coli dan Salmonella thypi. Pengujian daya hambat menggunakan metode Kirby-Bauer, yaitu metode difusi dengan cakram kertas dengan 5 perlakuan yaitu ekstrak daun kacapiring dengan konsentrasi 20%, 40%, dan 60% serta kontrol positif (ciprofloxacin) dan kontrol negatif (akuades). Berdasarkan hasil penelitian yang diperoleh, dapat disimpulkan bahwa ekstrak daun kacapiring dapat menghambat pertumbuhan bakteri Staphylococcus aureus, Escherichia coli dan Salmonella thypi pada konsentrasi 20%, 40% dan 60% dan tergolong dalam kategori kuat dan sedang. Kata Kunci  : Kacapiring, Antibakteri, Staphylococcus aureus, Escherichia coli, Salmonella thypi

scholarly journals Aktivitas Antibakteri Ekstrak Daun Widuri Terhadap Escherichia Coli Dan Staphylococcus Aureus

2020 ◽  
Vol 2 (1) ◽  
pp. 1-9
Author(s):  
Siti Zamilatul Azkiyah
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Bacterial Infections ◽  
Leaf Extract ◽  
Antibacterial Properties ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
The Body ◽  
And Staphylococcus Aureus

Antibiotics have a very important role in fighting bacterial infections in the body. However, the results given so far have not been optimal in overcoming the problem of bacterial infection. An alternative solution is to develop and study plants that have medicinal potential, for example thistle leaves (Calotrophis gigantean). The purpose of this study was to determine the antibacterial activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus. This research is a laboratory-scale experimental research that is to test the activity of thistle leaf extract against Escherichia coli and Staphylococcus aureus including sample identification, extraction, activity testing by observing the inhibition zone of the two tested bacteria. Based on the results of the study stated that the ethanol extract of 70% Widuri leaves (Calotrophis gigantea) had antibacterial activity and could inhibit the growth of Escherichia coli and Staphylococcus aureus bacteria. The active compounds that are thought to have antibacterial properties in the ethanol extract of Widuri leaves (Calotrophis gigantea) are flavonoids, alkaloids and terpenoids.   Keywords: Widuri leaves, Escherichia coli, Staphylococcus aureus, Antibacterial ABSTRAK   Pentingnya penanggulangan infeksi bakteri menggunakan antibiotik adalah hal yang lazim dalam terapi farmakologi. Dampak dari pemberian antibiotik masih belum maksimal dalam penanggulangannya terhadap infeksi bakteri. Pemilihan alternatif bahan alam dengan kandungan kimia yang memiliki potensi sebagai antibiotik dapat menjadi pilihan lain dalam mengurangi penggunaan antibiotik sintetik, misalnya tanaman widuri (Calotrophis gigantean). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri ekstrak daun widuri terhadap Escherichia coli dan Staphylococcus aureus. Desain penelitian meliputi identifikasi sampel, ekstraksi, hingga uji aktivitas dengan mengamati zona hambat dari kedua bakteri uji. Hasil penelitian menunjukkan bahwa ekstrak etanol 70% daun Widuri memiliki aktivitas antibakteri dan dapat menghambat pertumbuhan bakteri Escherichia coli dan Staphylococcus aureus. Senyawa aktif yang diduga bersifat antibakteri pada ekstrak etanol daun Widuri adalah senyawa golongan flavonoid, alkaloid dan terpenoid. Kata Kunci: Daun Widuri, Escherichia coli, Staphylococcus aureus, Antibakteri

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