scholarly journals Profil uretritis gonokokus dan uretritis non gonokokus di Poliklinik Kulit dan Kelamin RSUP Prof. Dr. R. D. Kandou Manado periode Januari – Desember 2013

e-CliniC ◽  
2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Jonna Palenewen ◽  
Ferra O. Mawu ◽  
Nurdjanah J. Niode
Keyword(s):  
Sexual Orientation ◽  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Descriptive Study ◽  
Sexual Contact ◽  
Gram Negative Bacteria ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Urethral Discharge ◽  
Gonococcal Urethritis

Abstract: Gonococcal urethritis (GU) is an inflammation of the urethra caused by Gram negative bacteria Neisseria gonorrhoeae associated with complaints of itching and burning sensation, pain during urination, mucopurulent urethral discharge from the tip of the urethra which sometimes bleeds, and pollakiuria. Non-gonococcal urethritis (NGU) is an inflammation of the urethra caused by Gram-negative bacterium Chlamydia trachomatis which is transmitted through sexual contact. Symptoms include mild dysuria, an uneasy feeling in the urethra, pollakiuria, and seropurulent urethral discharge. This study aimed to determine the profile of gonococcal urethritis and non-gonococcal urethritis in the Dermatovenereology Clinic of Prof. Dr. R. D. Kandou Hospital Manado from January-December 2013. This was a retrospective descriptive study based on the type of disease (GU or NGU), age, occupation, and sexual orientation. The results showed 204 cases of sexual transmitted infections (STIs). There were 27 cases of GU (6 %) and 10 cases of NGU (2 %). Most of GU and NGU patients were 25-44 years (17,46%) with private employment (17,47 %), and sexual orientation to heterosexual (37,10 %). Keywords: gonococcal urethritis, non-gonococcal urethritisAbstrak: Uretritis gonokokus (UG) ialah peradangan uretra disebabkan oleh bakteri Gram negatif Neisseria gonorrhoeae dengan keluhan gatal, panas, nyeri saat berkemih, dapat disertai keluar duh tubuh mukopurulen dari ujung uretra yang kadang mengeluarkan darah, dan polakisuria. Uretritis non gonokokus (UNG) ialah peradangan uretra disebabkan oleh bakteri Gram negatif Chlamydia trachomatis yang ditularkan melalui kontak seksual. Gejala berupa disuria ringan, perasaan tidak enak di uretra, polakisuria, dan keluarnya duh tubuh seropurulen. Penelitian ini bertujuan untuk mengetahui profil uretritis gonokokus dan non gonokokus di Poliklinik Kulit dan Kelamin RSUP Prof Dr. R. D. Kandou Manado periode Januari-Desember 2013. Jenis penelitian ini deskriptif retrospektif berdasarkan jenis penyakit (UG atau UNG), usia, pekerjaan, dan orientasi seksual. Hasil penelitian menunjukkan dari 204 kasus infeksi menular seksual (IMS) terdapat 27 kasus UG (6%) dan 10 kasus UNG (2%). Penderita terbanyak pada kelompok usia 25-44 tahun (17,46%), dengan pekerjaan swasta (17,47%), serta orientasi seksual dengan heteroseksual (37,100%).Kata kunci: uteritis gonokokus, uretritis non gonokokus

Urethritis

2020 ◽  
pp. 1606-1609
Author(s):  
Patrick Horner
Keyword(s):  
Chlamydia Trachomatis ◽  
Neisseria Gonorrhoeae ◽  
Partner Notification ◽  
Mycoplasma Genitalium ◽  
Urethral Discharge ◽  
Gonococcal Urethritis

Urethritis is defined as detectable urethral inflammation in the presence of symptoms or an observable urethral discharge. It is conventionally classified into gonococcal urethritis (GU, caused by Neisseria gonorrhoeae) and non-gonococcal urethritis (NGU, caused by Chlamydia trachomatis, Mycoplasma genitalium, and other causes, but with no known pathogen detected in over 30% of cases). Diagnosis is by urethral smear and microbiological investigations. Treatment with appropriate antibiotics should be given only to those with proven urethritis, and the diagnosis and its implications should be discussed with the patient. Partner notification is essential, not only to prevent re-infection but also to prevent onward transmission from partner(s) and the development of complications if left untreated.

scholarly journals Developing Cyclic Peptomers as Broad-Spectrum Gram negative Bacterial Type III Secretion System Inhibitors

2020 ◽  
Author(s):  
Hanh N. Lam ◽  
Tannia Lau ◽  
Adam Lentz ◽  
Jessica Sherry ◽  
Alejandro Cabrera-Cortez ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Chlamydia Trachomatis ◽  
Broad Spectrum ◽  
Type Iii Secretion ◽  
Type Iii Secretion System ◽  
Secretion System ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Type Iii ◽  
Bacterial Type

ABSTRACTAntibiotic resistant bacteria are an emerging global health threat. New antimicrobials are urgently needed. The injectisome type III secretion system (T3SS), required by dozens of Gram-negative bacteria for virulence but largely absent from non-pathogenic bacteria, is an attractive antimicrobial target. We previously identified synthetic cyclic peptomers, inspired by the natural product phepropeptin D, that inhibit protein secretion through the Yersinia Ysc and Pseudomonas aeruginosa Psc T3SSs, but do not inhibit bacterial growth. Here we describe identification of an isomer, 4EpDN, that is two-fold more potent (IC50 4 μM) than its parental compound. Furthermore, 4EpDN inhibited the Yersinia Ysa and the Salmonella SPI-1 T3SSs, suggesting that this cyclic peptomer has broad efficacy against evolutionarily distant injectisome T3SSs. Indeed, 4EpDN strongly inhibited intracellular growth of Chlamydia trachomatis in HeLa cells, which requires the T3SS. 4EpDN did not inhibit the unrelated Twin arginine translocation (Tat) system, nor did it impact T3SS gene transcription. Moreover, although the injectisome and flagellar T3SSs are evolutionarily and structurally related, the 4EpDN cyclic peptomer did not inhibit secretion of substrates through the Salmonella flagellar T3SS, indicating that cyclic peptomers broadly but specifically target the injestisome T3SS. 4EpDN reduced the number of T3SS basal bodies detected on the surface of Y. enterocolitica, as visualized using a fluorescent derivative of YscD, an inner membrane ring with low homology to flagellar protein FliG. Collectively, these data suggest that cyclic peptomers specifically inhibit the injectisome T3SS from a variety of Gram-negative bacteria, possibly by preventing complete T3SS assembly.IMPORTANCETraditional antibiotics target both pathogenic and commensal bacteria, resulting in a disruption of the microbiota, which in turn is tied to a number of acute and chronic diseases. The bacterial type III secretion system (T3SS) is an appendage used by many bacterial pathogens to establish infection, but is largely absent from commensal members of the microbiota. In this study, we identify a new derivative of the cyclic peptomer class of T3SS inhibitors. These compounds inhibit the T3SS of the nosocomial ESKAPE pathogen Pseudomonas aeruginosa and enteropathogenic Yersinia and Salmonella. The impact of cyclic peptomers is specific to the T3SS, as other bacterial secretory systems are unaffected. Importantly, cyclic peptomers completely block replication of Chlamydia trachomatis, the causative agent of genital, eye, and lung infections, in human cells, a process that requires the T3SS. Therefore, cyclic peptomers represent promising virulence blockers that can specifically disarm a broad spectrum of Gram-negative pathogens.

scholarly journals Neisseria gonorrhoeae Filamentous Phage Ngo 6 Is Capable of Infecting a Variety of Gram-Negative Bacteria

2013 ◽  
Vol 88 (2) ◽  
pp. 1002-1010 ◽  
Author(s):  
A. Piekarowicz ◽  
A. Klyz ◽  
M. Majchrzak ◽  
E. Szczesna ◽  
M. Piechucki ◽  
...  
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Filamentous Phage ◽  
Gram Negative Bacteria ◽  
Gram Negative

Non-gonococcal urethritis and mucopurulent cervicitis

2019 ◽  
pp. 161-168
Keyword(s):  
Antibiotic Resistance ◽  
Chlamydia Trachomatis ◽  
Clinical Features ◽  
Mycoplasma Genitalium ◽  
Common Cause ◽  
Urethral Discharge ◽  
Separate Section ◽  
Diagnosis And Management ◽  
Gonococcal Urethritis

Non-gonococcal urethritis (NGU) is caused by Chlamydia trachomatis in 11–50% of cases, but Mycoplasma genitalium is being increasingly recognized as a common cause. Mycoplasma genitalium is not as well understood, and experience with treatment is limited; however, antibiotic resistance is already a problem. Asymptomatic in up to 20% of men, symptoms include dysuria and urethral discharge. Mucopurulent cervicitis is the female equivalent, but is less well defined. This chapter describes the aetiology, clinical features, complications, and investigations and management of NGU, and mucopurulent cervicitis. Management of recurrent and persistent urethritis is also covered, including a separate section for diagnosis and management of Mycoplasma genitalium.

Fusobacterium nucleatum, the first Gram-negative bacterium demonstrated to produce polyglutamate

2009 ◽  
Vol 55 (5) ◽  
pp. 627-632 ◽  
Author(s):  
Thomas Candela ◽  
Marie Moya ◽  
Michel Haustant ◽  
Agnès Fouet
Keyword(s):  
In Silico Analysis ◽  
Fusobacterium Nucleatum ◽  
Gram Negative Bacteria ◽  
Negative Bacterium ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
The Third ◽  
Gram Negative Organisms ◽  
First Time

Poly-γ-glutamate has been described in many Gram-positive organisms. When anchored to the surface, it is a capsule and as such a virulence factor. Based on sequence similarities, few Gram-negative organisms have been suggested to synthesize poly-γ-glutamate. For the first time, a Gram-negative bacterium, Fusobacterium nucleatum , is shown to produce and secrete poly-γ-glutamate. Putative poly-γ-glutamate-synthesizing genes from Gram-negative organisms have been compared with their Gram-positive homologs by in silico analysis, i.e., gene sequence and phylogenetic analysis. Clusters of three instead of four genes were highlighted by our screen. The products of the first two genes display similarity with their Gram-positive equivalents, yet the sequences from the Gram-negative organisms can be distinguished from those of the Gram-positives. Interestingly, the sequence of the predicted product of the third gene is conserved among Gram-negative bacteria but displays no similarity to that of either the third or fourth gene of the Gram-positive operons. It is suggested that, like for Gram-positive bacteria, poly-γ-glutamate has a role in virulence for pathogens and one in survival for other Gram-negative bacteria.

scholarly journals Modulation of the Neisseria gonorrhoeae drug efflux conduit MtrE

2017 ◽  
Author(s):  
Giulia Tamburrino ◽  
Salomé Llabrés ◽  
Owen N. Vickery ◽  
Samantha J. Pitt ◽  
Ulrich Zachariae
Keyword(s):  
Neisseria Gonorrhoeae ◽  
Gram Negative Bacteria ◽  
Membrane Component ◽  
Gram Negative ◽  
Membrane Fusion Protein ◽  
Binding Interface ◽  
Open Conformation ◽  
Computational Electrophysiology ◽  
Maximum Conductance

ABSTRACTWidespread antibiotic resistance, especially of Gram-negative bacteria, has become a severe concern for human health. Tripartite efflux pumps are one of the major contributors to resistance in Gram-negative pathogens, by efficiently expelling a broad spectrum of antibiotics from the organism. In Neisseria gonorrhoeae, one of the first bacteria for which pan-resistance has been reported, the most expressed efflux complex is MtrCDE. Here we present the electrophysiological characterisation of the outer membrane component MtrE and the membrane fusion protein MtrC, obtained by a combination of planar lipid bilayer recordings and in silico techniques. Our in vitro results show that MtrE can be regulated by periplasmic binding events and that the interaction between MtrE and MtrC is sufficient to stabilize this complex in an open state. In contrast to other efflux conduits, the open complex only displays a slight preference for cations. The maximum conductance we obtain in the in vitro recordings is comparable to that seen in our computational electrophysiology simulations conducted on the MtrE crystal structure, indicating that this state may reflect a physiologically relevant open conformation of MtrE. Our results suggest that the MtrC/E binding interface is an important modulator of MtrE function, which could potentially be targeted by new efflux inhibitors.

scholarly journals Neisseria Meningitidis Urethritis in a Thai Male

2020 ◽  
Author(s):  
Jaray Tongtoyai ◽  
Nongkran Tatakham ◽  
Thitima Cherdtrakulkiat ◽  
Pachara Sirivongrangson ◽  
Andrew Hickey
Keyword(s):  
Sexual Health ◽  
Neisseria Meningitidis ◽  
Standard Treatment ◽  
Anal Intercourse ◽  
Sexual Contact ◽  
Health Clinic ◽  
Urethral Discharge ◽  
Gonococcal Urethritis ◽  
Urogenital Infections ◽  
Insertive Anal Intercourse

Although Neisseria meningitidis (N. meningitidis) urogenital infections have been reported widely, meningococcal urethritis has not been reported previously in Thailand. A 42-year-old Thai male presented at a sexual health clinic with dysuria and urethral discharge following oral and insertive anal intercourse. N. meningitidis, serogroup C was cultured from a urethral discharge specimen and the patient was treated successfully with standard treatment for gonococcal urethritis. This case reflects a growing trend of reports describing meningococcal urethritis, likely resulting from sexual contact.

scholarly journals Hen Fluorescein-Labeled Gonococcal Lipopolysaccharide Antibody in the Delayed Fluorescent Antibody Technique for the Confirmation of Neisseria gonorrhoeae

1979 ◽  
Vol 9 (3) ◽  
pp. 323-328
Author(s):  
F. E. Ashton ◽  
R. A. Leitch ◽  
M. B. Perry ◽  
R. Wallace ◽  
B. B. Diena
Keyword(s):  
Cell Surface ◽  
Neisseria Gonorrhoeae ◽  
Neisseria Meningitidis ◽  
Fluorescent Antibody ◽  
Fluorescent Antibody Technique ◽  
Gram Negative Bacteria ◽  
Antibody Technique ◽  
Gram Negative ◽  
Neisseria Lactamica ◽  
Antibody Conjugate

A fluorescent antibody reagent (termed anti-LPS conjugate) was prepared from sera obtained from hens immunized with gonococcal R-type lipopolysaccharide. The reagent was absorbed with Formalin-treated cells of Neisseria meningitidis. The anti-LPS conjugate gave uniform brilliant staining of Neisseria gonorrhoeae with little background fluorescence, thus making interpretation and reading of fluorescence simple. The conjugate did not significantly stain cultures of N. meningitidis, Neisseria lactamica , nonpathogenic Neisseria species, or other gram-negative bacteria. Several preparations of the conjugate provided the same specificity and reproducibility of staining. The anti-LPS conjugate was compared with Difco Laboratories fluorescent antibody conjugate for staining of N. gonorrhoeae. Both conjugates stained cells of the light and dark variants of gonococcal colony types 1 and 2, as well as cells of colony types 3 and 4. When used for the confirmation of N. gonorrhoeae , the anti-LPS and Difco conjugates stained 426 of 431 (98.8%) and 210 of 213 (98.6%) of the gonococcal cultures, respectively. Absorption of the anti-LPS conjugate with R-type lipopolysaccharide removed the staining of gonococci. However, absorption of Difco conjugate with R-type lipopolysaccharide did not remove the staining of gonococci, suggesting that the majority of fluorescein-labeled antibody present in the Difco conjugate is directed to gonococcal cell surface components other than lipopolysaccharide. The results of this study indicate that fluorescein-labeled gonococcal lipopolysaccharide antibody should be a reliable fluorescent antibody reagent for the confirmation of N. gonorrhoeae.

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