UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI KARANG LUNAK (Sarcophyton sp.) DARI PERAIRAN PULAU BANGKA LIKUPANG TERHADAP PERTUMBUHAN  MIKROBA Staphylococcus aureus, Salmonella tyhpimurium, DAN Candida albicans

ABSTRACTSarcophyton sp. is able to produce bioactive materials, the bioactive material contained in Sarcophyton sp., is sarcophine. The purpose of this study is to find out whether the extracts from soft coral Sarcophyton sp., in Bangka Island waters Likupang performed antimicrobial activity against several pathogenic microbes, such as Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. Samples were extracted by maceration method with ethanol solvent and the fractionation method used was liquid-liquid.The antimicrobial activity testing method used in this study is the Kirby and Bauer disc diffusion method. The results obtained from the antimicrobial activity test on Staphylococcus aureus, fractions of n-hexane with result 8.27mm and fractions of chloroform 8.41mm. In Candida albicans , the n-hexane fraction has an 8.18mm and the chloroform fraction 8.06. While for Salmonella typhimurium has no activity. The conclusion of this study it was found that only some fractions have antimicrobial activity and categorized as moderate, such as the fractions of n-hexane and chloroform that only have moderate inhibiting activity against Stapphylococcus aureus and Candida albicans while for Salmonella typhimurium, the extracts and fractions of Sarcophyton sp., has no activity to the way to inhibit these bacteria. Keywords: Sarcophyton sp., Antimicrobial, Staphylococcus aureus, Salmonella tyhpimurium, Candida albicans ABSTRAK Sarcophyton sp. mampu menghasilkan bahan bioaktif, bahan bioaktif yang terdapat pada Sarcophyton sp adalah sarcophine. Tujuan dari penelitian ini yaitu untuk mengetahui apakah ekstrak dari Karang Lunak Sarcophyton sp.,di Perairan Pulau Bangka Likupang memiliki aktivitas antimikroba terhadap beberapa mikroba patogen Staphylococcus aureus, Salmonella typhi, dan Candida albicans. Sampel diekstraksi dengan metode maserasi dengan pelarut etanol dan metode fraksinasi yang digunakan yaitu fraksinasi cair – cair. Metode pengujian aktivitas antimikroba yang digunakan dalam penelitian ini yaitu metode difusi agar (disc diffusion Kirby and Bauer). Hasil yang didapat dari uji aktivitas antimikroba pada bakteri Staphylococcus aureus, fraksi n-heksan mendapatkan hasil 8.27mm dan pada fraksi kloroform 8.41mm. Pada jamur Candida albicans, fraksi n-heksan mendapatkan hasil 8.18mm dan pada fraksi kloroform 8.06mm, sedangkan pada bakteri Salmonella typhi tidak memiliki aktivitas. Kesimpulan dari penelitian ini, hanya sebagian fraksi yang memiliki aktivitas untuk penghambat antimikroba dengan dikategorikan sedang, seperti pada fraksi n-heksan dan klorofom pada bakteri Stapphylococcus aureus dan jamur Candida albicans, sementara untuk bakteri Salmonella tyhpi ekstrak dan fraksi sampel Karang Lunak Sarcophyton sp. tidak memiliki aktivitas untuk menghambat bakteri tersebut. Kata Kunci : Sarcophyton sp., antimikroba, Staphylococcus aureus, Salmonella tyhpimurium, Candida albicans

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UJI AKTIVITAS ANTIMIKROBA DARI EKSTRAK DAN FRAKSI ASCIDIAN Herdmania momus DARI PERAIRAN PULAU BANGKA LIKUPANG TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Salmonella typhimurium DAN Candida albicans

PHARMACON ◽

10.35799/pha.10.2021.32758 ◽

2021 ◽

Vol 10 (1) ◽

pp. 706

Author(s):

Novira Vita Wendersteyt ◽

Defny S. Wewengkang ◽

Surya Sumantri Abdullah

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Salmonella Typhimurium ◽

Microbial Growth ◽

Ethanol Extract ◽

Diffusion Method ◽

Chloroform Fraction ◽

Methanol Fraction ◽

Potential Source

ABSTRACT Ascidian is one of the components of coral reefs known as a potential source of exploration for the search for new bioactive compounds as potential drugs for the pharmaceutical world. This study aims to determine the antimicrobial activity of the extracts and fractions of Ascidian Herdmania momus collected from the waters of Bangka Island Likupang against the microbial growth of Staphylococcus aureus, Salmonella typhimurium, and Candida albicans. Samples were extracted by maceration using ethanol and then fractionated using n-hexane, chloroform and methanol solvent, respectively. Antimicrobial activity test was carried out by agar diffusion method (Kirby and Bauer). The results showed that the ethanol extract, n-hexane fraction, chloroform fraction, and methanol fraction were not active in inhibiting the growth of Staphylococcus aureus and Salmonella typhimurium but were quite active in inhibiting the growth of Candida albicans in the chloroform and methanol fraction based on the Davis and Stout category. Keywords: Ascidian Herdmania momus, antimicrobial activity, Staphylococcus aureus, Salmonella typhimurium, Candida albicans ABSTRAK Ascidian merupakan salah satu biota penyusun terumbu karang yang diketahui berpotensi sebagai sumber eksplorasi pencarian senyawa bioaktif baru sebagai calon obat untuk dunia Farmasi. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba dari ekstrak dan fraksi Ascidian Herdmania momus yang diambil dari perairan Pulau Bangka Likupang terhadap pertumbuhan mikroba Staphylococcus aureus, Salmonella typhimurium, dan Candida albicans. Sampel diekstraksi dengan cara maserasi menggunakan pelarut Etanol. Fraksinasi menggunakan pelarut n-Heksan, Kloroform dan Metanol. Uji aktivitas antimikroba dilakukan dengan metode difusi agar (Kirby dan Bauer). Hasil penelitian menunjukkan bahwa ekstrak Etanol, fraksi n-Heksan, fraksi Kloroform, dan Fraksi Metanol tidak efektif menghambat pertumbuhan mikroba Staphylococcus aureus dan Salmonella typhimurium namun cukup efektif menghambat pertumbuhan mikroba Candida albicans pada fraksi Kloroform dan Metanol dengan kategori sedang berdasarkan teori Davis dan Stout. Kata Kunci: Ascidian Herdmania momus, aktivitas antimikroba, Staphylococcus aureus, Salmonella typhimurium, Candida albicans

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UJI AKTIVITAS EKSTRAK DAN FRAKSI TUNIKATA Lissoclinum patella TERHADAP PERTUMBUHAN MIKROBA Escherichia coli, Staphylococcus aureus, DAN Candida albicans

PHARMACON ◽

10.35799/pha.8.2019.29359 ◽

2019 ◽

Vol 8 (4) ◽

pp. 825

Author(s):

Yunike E. Ngantung ◽

Herny E. I. Simbala ◽

Henki Rotinsulu

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Diffusion Method ◽

Chloroform Fraction ◽

Marine Biota ◽

Disc Diffusion ◽

Solvent Fractionation ◽

Lissoclinum Patella

ABSTRACT Tunicate is one of the marine biota that has the potential to be utilized as a search for bioactive compounds, as prospective drugs in the pharmaceutical world. This study aims to determine the antimicrobial activity of extracts and fractions of Tunicates Lissoclinum patella obtained from the waters of Tumbak Village against the growth of microbes Escherichia coli, Staphylococcus aureus and Candida albicans. Samples were extracted by maceration using ethanol as a solvent. Fractionation using n-hexane, chloroform and methanol. Antimicrobial activity was tested using the disc diffusion method (Kirby and Bauer). The results showed that the ethanols extract, n-hexane fraction, chloroform fraction, methanol fraction effectively inhibited the growth of microbes Escherichia coli, Staphylococcus aureus, Candida albicans with moderate inhibition strength categories based on Davis and Stout theory. Keywords : Tunicata, Lissoclinum patella, antimicrobial, Candida albicans, Escherichia coli, Staphylococcus aureus. ABSTRAK Tunikata merupakan salah satu biota laut yang berpotensi untuk dimanfaatkan sebagai bahan pencarian senyawa bioaktif, calon obat di dunia farmasi. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba dari ekstrak dan fraksi Tunikata Lissoclinum patella yang diperoleh dari perairan Desa Tumbak terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus dan Candida albicans. Sampel diekstraksi dengan cara maserasi menggunakan pelarut etanol. Fraksinasi menggunakan pelarut n-Heksan, kloroform dan metanol. Aktivitas antimikroba diuji menggunakan metode disc diffusion test (Kirby dan Bauer). Hasil penelitian menunjukkan bahkwa ekstrak etanol, fraksi n-Heksan, fraksi kloroform, fraksi metanol efektif menghambat mikroba Escherichia coli, Staphylococcus aureus, Candida albicans dengan kategori kekuatan daya hambat sedang berdasarkan teori Davis dan Stout. Kata kunci : Tunikata, Lissoclinum patella, antimikroba, Candida albicans, Escherichia coli, Staphylococcus aureus.

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UJI DAYA HAMBAT EKSTRAK DAN FRAKSI SPONS Aaptos Aaptos TERHADAP PERTUMBUHAN Escherichia coli, Staphylococcus aureus, dan Candida albicans

PHARMACON ◽

10.35799/pha.8.2019.29251 ◽

2019 ◽

Vol 8 (1) ◽

pp. 168

Author(s):

Sitti N Tunggali ◽

Herny E. I. Simbala ◽

Henki Rotinsulu

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Microbial Growth ◽

Ethanol Extract ◽

Chloroform Fraction ◽

Marine Biota ◽

Disc Diffusion ◽

Average Value

ABSTRACT Sponge Aaptos aaptos is a marine biota that has great potential, which can be applied, in the pharmaceutical field because of the presence of large compounds in inhibiting microbial growth. This study aims to determine the inhibitory activity of extracts and fractions of sponge Aaptos aaptos on microbial growth of Escherichia coli, Staphylococcus aureus, and Candida albicans. The samples were extracted by maceration with 96 % ethanol and fractioned with n-hexane, choloroform and methanol. Testing is done using the Disc Diffusion Agar method. Crude ethanol extract and fraction of sponge Aaptos aaptos showed the greatest antimicrobial activity against Staphylococcus aureus and categorized as strong, with an average value of 20.32 mm for ethanol extract with strong categories, chloroform fraction 13,28 mm with medium category and methanol fractions 18,48 mm strong category. Keyword: Aaptos aaptos, antimicrobial activity, Escherichia coli, Staphylococcus aureus, Candida albicans. ABSTRAK Spons Aaptos aaptos merupakan biota laut yang memiliki potensi sebagai antimikroba yang dapat diterapkan di bidang farmasi dengan kandungan senyawa yang besar dalam menghambat pertumbuhan mikroba. Penelitian ini bertujuan untuk mengetahui aktivitas daya hambat dari ekstrak dan fraksi spons Aaptos aaptos terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus, dan Candida albicans. Sampel diekstraksi secara maserasi dengan etanol dan difraksinasi dengan pelarut n–heksan, kloroform dan metanol. Pengujian dilakukan dengan menggunakan metode Disc Diffusion Agar. Ekstrak kasar etanol dan fraksi dari Spons Aaptos aaptos menunjukkan aktivitas antimikroba paling besar terhadap Staphylococcus aureus dan dikategorikan kuat, dengan nilai rata – rata 20,32 mm untuk ekstrak etanol dengan kategori kuat, fraksi kloroform 13,28 mm, kategori sedang dan fraksi metanol 18,48 mm kategori kuat.Kata Kunci : Aaptos aaptos, aktivitas antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans

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UJI AKTIVITAS ANTIMIKROBA EKSTRAK DAN FRAKSI SPONS Liosina paradoxa DARI PERAIRAN DESA TUMBAK MINAHASA TENGGARA TERHADAP PERTUMBUHAN MIKROBA Staphylococcus aureus, Escherichia coli, DAN Candida albicans

PHARMACON ◽

10.35799/pha.8.2019.29386 ◽

2019 ◽

Vol 8 (3) ◽

pp. 629

Author(s):

Walen E. Oeiyano ◽

Herny E. I. Simbala ◽

Henki Rotinsulu

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Diffusion Method ◽

Chloroform Fraction ◽

Growth Inhibitors ◽

Marine Animals ◽

Average Value ◽

Ethanol Extracts

ABSTRACT Sponges are marine animals, which contain active compounds known to have potential in the pharmaceutical field, including the antimicrobials. The purpose of this study was to determine the activity of microorganism growth inhibitors from the sponge Liosina paradoxa against microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Samples were extracted by maceration method with ethanol solvent and fractionation using methanol, chloroform, and n-hexane solvents. Antimicrobial activity was carried out by agar diffusion method (Kirby and Bauer). Methanol fractions of sponge Liosina paradoxa showed the greatest antimicrobial activity against Escherichia coli with an average value of 11.33 mm, and categorize as strong, chloroform fraction was 7.33 mm and categorize as medium, and n-hexane fraction was 7.16 mm and categorize as medium, ethanol extracts was 7.83 mm and categorize as moderate antimicrobial activity. Keywords: Liosina paradoxa, Antimicrobial, Staphylococcus aureus, Escherichia coli, Candida albicans. ABSTRAKSpons merupakan merupakan hewan laut yang mengandung senyawa aktif yang diketahui berpotensi dalam bidang farmasi, diantaranya sebagai antimikroba. Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas penghambat pertumbuhan mikroorganisme dari spons Liosina paradoxa terhadap mikroba Staphylococcus aureus, Escherichia coli, dan Candida albicans.Sampel diekstraksi dengan metode maserasi dengan pelarut etanol dan fraksinasi menggunakan pelarut metanol, kloroform, dan n-heksan. Aktivitas antimikroba dilakukan dengan metode difusi agar (Kirby and Bauer). Fraksi metanol spons Liosina paradoxa menunjukkan aktivitas antimikroba paling besar terhadap Escherichia coli dengan nilai rata-rata 11,33 mm, dengan kategori kuat, fraksi kloroform 7,33 mm kategori sedang, fraksi n-hexan 7,16 mm kategori sedang, ekstrak etanol 7,83 mm kategori aktivitas antimikroba sedang. Kata kunci: Liosina paradoxa, antimikroba, Staphylococcus aureus ,Escherichia coli, Candida albicans.

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UJI AKTIVITAS ANTIMIKROBA EKSTRAK ETANOL ORGANISME LAUT SPONS Ianthella basta TERHADAP BEBERAPA MIKROBA PATOGEN

PHARMACON ◽

10.35799/pha.9.2020.27415 ◽

2020 ◽

Vol 9 (1) ◽

pp. 100

Author(s):

Syifa Sari Katili ◽

Defny S Wewengkang ◽

Henki Rotinsulu

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Marine Organism ◽

Ethanol Extract ◽

Diffusion Method ◽

Disc Diffusion ◽

Paper Disc

ABSTRACTSponges are multicellular metazoa animals belonging to the Porifera phylum, which has a different structure from other metazoans. The purpose of this study was to determine whether ethanol extracts from the marine organism sponge Ianthella basta have antimicrobial activity against several pathogenic microbes Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. The extraction method used is maceration with 96% ethanol solvent. The method used is the Diffusion Method (Disc Diffusion Kirby and Bauer). The antimicrobial activity test uses a 6 mm paper disc with 50 µL absorption per disc. The results of crude ethanol extract of Ianthella basta sponge from all test microbes, namely Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans, were seen to provide the greatest inhibitory activity against Staphylococcus aureus bacteria with an average of inhibitory zone of 7.00 mm categorize as intermediate The results obtained showed that the crude extract of the sponge Ianthella basta has antimicrobial activity because it can inhibit the growth of Staphylococcus aureus, Escherichia coli and Candida albicans microbes even though the inhibition zone is categorized as intermediate. Keywords: Ianthella basta, antimicrobial, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans. ABSTRAKSpons adalah hewan metazoa multiseluler tergolong ke dalam filum Porifera, yang memiliki perbedaan struktur dengan metazoan lainnya. Tujuan penelitian ini ialah untuk mengetahui apakah ekstrak etanol dari organisme laut spons Ianthella basta memiliki aktivitas antimikroba terhadap beberapa mikroba patogen Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa dan Candida albicans. Metode ekstraksi yang digunakan adalah maserasi dengan pelarut etanol 96%. Metode yang digunakan yaitu Metode Difusi (Disc Diffusion Kirby and Bauer). Pengujian aktivitas antimikroba ini menggunakan kertas cakram (paper disc) berukuran 6 mm dengan daya serap 50 µL tiap cakram. Hasil ekstrak kasar etanol Spons Ianthella basta dari semua mikroba uji yaitu Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa dan Candida albicans, terlihat yang memberikan daya hambat paling besar terdapat pada bakteri Staphylococcus aureus dengan jumlah rata – rata zona hambat yaitu 7,00 mm dengan kategori sedang. Hasil penelitian yang diperoleh menunjukkan bahwa ekstrak kasar dari Spons Ianthella basta memiliki aktivitas antimikroba karena mampu menghambat pertumbuhan mikroba uji Staphylococcus aureus, Escherichia coli dan Candida albicans walaupun dengan zona hambat yang dikategorikan sedang. Kata Kunci : Ianthella basta, antimikroba, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Candida albicans.

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UJI ANTIMIKROBA EKSTRAK DAN FRAKSI TUNIKATA Didemnum molle TERHADAP PERTUMBUHAN Escherichia coli, Staphylococcus aureus, DAN Candida albicans YANG DIKOLEKSI DI SELAT LEMBEH BITUNG

PHARMACON ◽

10.35799/pha.8.2019.29292 ◽

2019 ◽

Vol 8 (2) ◽

pp. 275

Author(s):

Novia A. Pitoy ◽

Adithya Yudistira ◽

Defny S. Wewengkang

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Microbial Growth ◽

Test Method ◽

Chloroform Fraction ◽

Marine Biota ◽

Disc Diffusion ◽

Diffusion Test

ABSTRACTTunicate Didemnum molle is a marine biota that has bioactive components that can be used as raw material for medicines. This study aims to determine the antimicrobial activity of extracts and fractions of Didemnum molle on microbial growth of Escherichia coli, Staphylococcus aureus and Candida albicans. The sample was extracted by maceration using ethanol and fractionated using, n-hexane, chloroform and methanol. Antimicrobial activity was tested using the disc diffusion test method (Kirby and Bauer). Ethanol extract of Didemnum molle inhibited the growth of microbes of Staphylococcus aureus (8.12 mm) and Candida albicans (9.00 mm). Chloroform fraction inhibited the growth of Staphylococcus aureus (9.10 mm), Escherichia coli (10.00 mm) and Candida albicans (9.65 mm). While the methanol fraction is only able to inhibit microbial growth of Candida albicans (10.00 mm). The hexane fraction shows no activity against all test microbes. Keywords : Tunikata Didemnum molle, antimicrobial, Escherichia coli, Staphylococcus aureus, Candida albicans. ABSTRAKTunikata Didemnum molle merupakan biota laut yang memiliki komponen bioaktif yang dapat dimanfaatkan sebagai bahan baku obat-obatan. Penelitian ini bertujuan untuk mengetahui aktivitas antimikroba dari ekstrak dan fraksi tunikata Didemnum molle terhadap pertumbuhan mikroba Escherichia coli, Staphylococcus aureus dan Candida albicans. Sampel diekstraksi dengan cara maserasi menggunakan etanol dan difraksinasi menggunakan, n-heksan, kloroform dan metanol. Aktivitas antimikroba diuji menggunakan metode disc diffusion test (Kirby dan Bauer). Ekstrak etanol Didemnum molle menghambat pertumbuhan mikroba Staphylococcus aureus (8,12 mm) dan Candida albicans (9,00 mm). Fraksi kloroform menghambat pertumbuhan mikroba Staphylococcus aureus (9,10 mm), Escherichia coli (10,00 mm) dan Candida albicans (9,65 mm). Sementara fraksi metanol hanya mampu menghambat pertumbuhan mikroba Candida albicans (10.00 mm). Fraksi heksan tidak menunjukkan aktivitas terhadap semua mikroba uji. Kata Kunci : Tunikata Didemnum molle, antimikroba, Escherichia coli, Staphylococcus aureus, Candida albicans.

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The In vitro Evaluation of the Antimicrobial Activity of Quercus robur L. Methanolic and Aqueous Leaves’ Extracts, from the Algerian High Plateaus Against some Uropathogenic Microbial Strains

Phytothérapie ◽

10.3166/phyto-2019-0133 ◽

2019 ◽

Vol 18 (5) ◽

pp. 262-274

Author(s):

E. Benyagoub ◽

N. Nabbou ◽

S. Boukhalkhel ◽

I. Dehini

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Enterococcus Faecalis ◽

Fusidic Acid ◽

Quercus Robur ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Microbial Strains

The medicinal value of the plants is due to their chemical components that bring a definite physiological action on the human body to prevent the diseases. In this work, we investigated the antimicrobial activity of leaves’ extracts of Quercus robur L., collected from the Algerian upper highlands, on ten bacterial strains and one fungal strain known to be pathogenic. First, we performed a qualitative phytochemical analysis, and second, antimicrobial activity tests performed by agar diffusion method (disc and well) with the determination of MIC by broth macro-dilution method. Given the results, it appears that obtained macerates of Quercus robur L. were rich in bioactive phytoconstituents such as alkaloids, anthraquinones, saponins, tannins, and other components. The yield of aqueous and methanolic macerates of leaves was 8.5 ± 1.41 and 22.4 ± 4.36%, respectively. The bacterial resistance was relatively important to several antibiotics, namely, ampicillin, amoxicillin + clavulanic acid for strains of Escherichia coli and Salmonella sp. However, Staphylococcus aureus strains were resistant to fusidic acid, penicillin, and oxacillin; while Enterococcus faecalis was resistant to fusidic acid, penicillin, oxacillin, and ticarcillin. The antibacterial activity of the macerates toward tested microbial strains showed that the aqueous and methanolic macerates of the leaves were proportional to the tested concentration and active not only against Gram-positive and Gram-negative bacteria but also on the fungal species Candida albicans. The estimated MIC for Escherichia coli, Enterococcus faecalis, and Staphylococcus aureus was in the order of 10 mg/mL, which seems more effective than toward Salmonella sp., Klebsiella pneumoniae, Pseudomonas aeruginosa, and Candida albicans which were in the order of 30 mg/mL. These preliminary results confirm that the part of the studied plant had a very good antimicrobial activity that was proportional to the serial concentrations of the tested extracts.

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BIOAKTIVITAS JAMUR Aspergillus flavus YANG BERSIMBION DENGAN ASCIDIAN Eudistoma sp.

JURNAL PESISIR DAN LAUT TROPIS ◽

10.35800/jplt.8.2.2020.28765 ◽

2020 ◽

Vol 8 (2) ◽

pp. 11

Author(s):

Alfa Mongi ◽

Deiske A Sumilat ◽

Fitje Losung ◽

Remmy E P Mangindaan ◽

Rosita A Lintang ◽

...

Keyword(s):

Escherichia Coli ◽

Staphylococcus Aureus ◽

Candida Albicans ◽

Ethyl Acetate ◽

Aspergillus Flavus ◽

Marine Invertebrate ◽

Diffusion Method ◽

Disc Diffusion ◽

Antibacterial And Antifungal ◽

Ethyl Acetate Extracts

Ascidian is a marine invertebrate that produce bioactive compounds such as antibacterial and antifungal. The purpose of this study was to obtain symbiotic fungi isolates, ethyl acetate extracts from ascidian symbiotic isolates, and to determine the antibacterial and antifungal activities of ethyl acetate extracts of symbiotic isolates using the diffusion method (disc diffusion Kirby and Bauer) against Escherichia coli, Staphylococcus aureus bacteria and against the Candida albicans fungus. The results obtained 1 ascidian species which was cultured to obtain pure isolates, the pure isolates were tested molecularly and the results showed that the isolate was Aspergillus flavus, then the isolates were grown on rice medium and extracted. The results of the fungal extract showed the presence of antibacterial and antifungal activity against Escherichia coli by 10 mm, Staphylococcus aureus by 19 mm and against the Candida albicans by 13.5 mm. Keywords : Ascidian, Eudistoma sp., Aspergillus flavus, Antibacterial, Antifungal, Symbiotic. AbstrakAscidian merupakan avertebrata laut yang dapat menghasilkan senyawa bioaktif yang seperti antibakteri dan antijamur. Tujuan dari penelitian ini yaitu mendapatkan isolat jamur simbion dari ascidian, mendapatkan ekstrak etil asetat dari isolat jamur simbion ascidian, dan menguji aktivitas antibakteri dan antijamur ekstrak etil asetat isolat jamur simbion dengan metode difusi agar (disc diffusion Kirby and Bauer) terhadap bakteri Escherichia coli, Staphylococcus aureus dan terhadap jamur Candida albicsans. Hasil penelitian didapatkan 1 jenis ascidian yang kemudian dikultur untuk mendapatkan isolat murni, isolat murni diuji secara molekuler dan didapati hasil bahwa isolat tersebut merupakan jamur Aspergillus flavus. Isolat tersebut kemudian ditumbuhkan pada media nasi dan diekstrak. Hasil ekstrak jamur tersebut menunjukkan adanya aktivitas antibakteri dan antijamur terhadap bakteri Escherichia coli sebesar 10 mm, Staphylococcus aureus sebesar 19 mm dan terhadap jamur Candida albicsans 13,5 mm. Kata kunci : Ascidian, Eudistoma sp., Aspergillus flavus, Antibakteri, Antijamur, Simbion.

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Characterization and Antimicrobial Activity of Erbium(III) Complexes of C-3 Substituted 2-hydroxy-1,4-naphthalenedione-1-oxime Derivatives

Metal-Based Drugs ◽

10.1155/mbd.2001.159 ◽

2001 ◽

Vol 8 (3) ◽

pp. 159-164 ◽

Cited By ~ 4

Author(s):

S. B. Jagtap ◽

N. N. Patil ◽

B. P. Kapadnis ◽

B. A. Kulkarni

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Antimicrobial Activity ◽

Antibacterial Activity ◽

Magnetic Susceptibility ◽

Candida Albicans ◽

Xanthomonas Campestris ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Oxime Derivatives

Erbium(III) complexes of 2-hydroxy-l,4-naphthalenedione-1-oxime and its C-3 substituted derivatives are synthesized and characterized by elemental analysis, thermogravimetric analysis, infrared spectroscopy, magnetic susceptibility measurements 2-hydroxy-1,4-naphthalenedione-1-oxime derivatives are analysed using H1 and C13 NMR spectroscopy. The molecular composition of the synthesized complexes is found to be [ML3(H2O)2]. The antimicrobial activity of these complexes is determined by well diffusion method against the target microorganisms- Staphylococcus aureus, Xanthomonas campestris, Pseudomonas aeruginosa, Candida albicans and Aspergillus niger. The antimicrobial activities of 2- hydroxy-1,4-naphthalenedione-1-oximes and their complexes are compared. It is observed that 2-hydroxy-1,4-naphthalenedione-l-oximes exhibit higher antifungal activity as compared to antibacterial activity. These activities are reduced upon complexation of these oximes with Erbium.

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Screening of the antimicrobial activity of marine sponge extracts from Martinique

10.1101/2021.02.16.431398 ◽

2021 ◽

Author(s):

Julie Piron ◽

Jessica Pastour ◽

Niklas Tysklind ◽

Juliette Smith-Ravin ◽

Fabienne Priam

Keyword(s):

Staphylococcus Aureus ◽

Antimicrobial Activity ◽

Inhibitory Concentration ◽

Minimum Bactericidal Concentration ◽

Marine Sponges ◽

Diffusion Method ◽

Disc Diffusion ◽

Bacterial Strains ◽

Disc Diffusion Method ◽

Staphylococcus Saprophyticus

AbstractMarine sponges are known for their antimicrobial, antifungal, and cytotoxic activity. In this study, the activity of aqueous and ethanoic extracts of 3 sponges from Martinique were tested on 5 bacterial strains: Bascillus cereus (CIP 783), Echerichia coli (CIP 54127), Pseudomonas aeruginosa (CIP A22), Staphylococcus aureus (CIP 67.8) and Staphylococcus saprophyticus (CIP 76125). The antimicrobial activity of Agelas clathrodes, Desmapsamma anchorata, and Verongula rigida, was demonstrated using the disc diffusion method and by determining the minimum inhibitory concentration and the minimum bactericidal concentration. The ethanoic extract of Agelas clathrodes had an inhibitory activity specifically on Staphylococcus aureus and Staphylococcus saprophyticus. No activity was observed for the other extracts. Further chemical analyses will be carried out in order to identify the active molecules of these sponges.

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