SEROLOGICAL AND MOLECULAR DIAGNOSIS OF TOXOPLASMA GONDII AMONG EWES AND HORSES IN DUHOK PROVINCE-IRAQ

2020 ◽  
Vol 51 (4) ◽  
pp. 1212-1219
Author(s):  
Mikaeel & Al-Saeed
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Indirect Elisa ◽  
Molecular Study ◽  
The Other ◽  
Blood Samples ◽  
B1 Gene ◽  
History Of ◽  
Positive Results ◽  
Age And Sex

This study was aimed to demonstrate the seroprevalence and molecular detection of Toxoplasma gondii among ewes and horses as well as to determine the risk factors for infection in Duhok province. Sera and blood samples from 700 ewes and 62 horses were collected. Sera were examined by indirect ELISA for detection of anti-T. gondii IgM antibodies and in molecular study, DNA was extracted, then by PCR, B1 gene was amplified and the product visualized and sent off for sequencing. Serologically, the prevalence of toxoplasmosis was 17.7 (11/62) and 28.9 (202/700) in horses and ewes by ELISA respectively. Present of cats on the farm was significantly associated with T. gondii infected ewes in the farm. While, Age, number of abortion and history of abortion has no role in infection in ewes. On the other hand, age and sex have no role in prevalence of toxoplasmosis among the horses. Only 2 samples among 11 seropositive samples by ELISA were give positive results by PCR in rate 18.2% in horse, while in ewes 13 samples from 60 randomly selected seropositive by ELISA were found to be positive by PCR in rate 21.7%. Results of this study indicate that prevalence of T. gondii among ewes and horses was high and cats have a role in prevalence of infection among the ewes.

Molecular detection of the B1 gene of Toxoplasma gondii in blood samples of female sheep and goats in Tebessa, northeastern Algeria

2020 ◽  
Vol 72 ◽  
pp. 101530
Author(s):  
Nassima Ait Issad ◽  
Khaled Abdelouahed ◽  
Salim Bekhouche ◽  
Racha Boubeuker ◽  
Haiet Hamoudi Adjmi ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Blood Samples ◽  
Sheep And Goats ◽  
B1 Gene ◽  
Female Sheep ◽  
Northeastern Algeria

scholarly journals Genotypic detection of some virulence factors among Aeromonas hydrophila Isolated from Diarrhea Cases (Iraq)

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Ilham A Bunyan ◽  
Israa K Obais
Keyword(s):  
Virulence Factors ◽  
Aeromonas Hydrophila ◽  
Molecular Detection ◽  
Virulence Gene ◽  
Molecular Study ◽  
Protease Gene ◽  
Allelic Ladder ◽  
Specific Pcr ◽  
Molecular Length ◽  
Positive Results

The present study included the detection ofsome virulence factorsof Aeromonas hydrophila under molecular level to clinical isolates were taken from patients suffering from diarrhea during the period from July (2017) to October (2017). Molecular detection of Hemolysin gene (ahh) was done for all isolates. The results showed that all isolates (100%) gave positive results for this virulence gene. the positive results were detected by the presence of (130) bp bands when compared with allelic ladder. The genomic DNA of the samples was extracted and bands were observed by performing agarose gel electrophoresis. When PCR was performed,results clearly indicate that all isolated organisms contained serine protease gene and all the amplified products produced a band at the level of (900 bp) when compared with the allelic ladder. Molecular detection of this gene was carried out by using a specific PCR primer were done by comparison with allelic ladder which gave a (309bp) It was found that (Aerolysin) gene present in (12) (75%) of the positive samples. Lip gene was also detected in A. hydrophila samples and found that all 16 samples (100%) gave positive results to this gene which gave molecular length (382) bp. Molecular study was carried out to show the sequence identity of cytotonic enterotoxins gene in Aeromonas spp. to that in A. hydrophila. Analysis of the A. hydrophila genome revealed a number of a putative virulence factors,including a gene that heat-labile cytotonic enterotoxin (alt). Our study showed that all (16) isolates (100%) gave positive results to this gene,which gave molecular length (442)bp. Molecular detection of cytotonic enterotoxins gene (ast) was done for (16) A. hydrophila isolates and the results showed that all isolates have this gene (100%). The positive results for (ast) virulence were detected by the presence of (331) bp band compared with allelic ladder.

scholarly journals Molecular detection of Babesia bovis in cattle in Al-Qadisiyah province

2017 ◽  
Vol 40 (2) ◽  
pp. 155-158
Author(s):  
Noaman N. A,aiz
Keyword(s):  
Polymerase Chain Reaction ◽  
Infection Rate ◽  
Molecular Detection ◽  
Babesia Bovis ◽  
Chain Reaction ◽  
Adult Cattle ◽  
Blood Samples ◽  
Significant Difference ◽  
Polymerase Chain ◽  
Age And Sex

     This study aim to determine Babesia bovis infection in cattle based on genetic methods. A total of 96 blood samples were collected from alive and slaughtered cattle from different areas in addition to the abattoir of Al-Qadisiyah province from December 2013 to August 2014. Real time polymerase chain reaction (RT.PCR) technique was used to detect the presence of the protozoan with the effect of animal's age and sex in the infection rate 47.91 % (46/96) of examined cattle were given positive result to B. bovis infection. The highest infections were shown among the adult cattle (≥1 year), while there was non-significant difference (P>0.05) in the infection rate according to the sex. So the most cattle in Al-Qadisiyah province appear to be bearing the infection predominantly as a carrier hosts.

Platelet Dysfunction in Migraine: Effect of Self-Medication with Aspirin

1976 ◽  
Vol 36 (02) ◽  
pp. 319-324 ◽  
Author(s):  
Sunanda V. Deshmukh ◽  
John Stirling Meyer ◽  
Richard J. Mouche
Keyword(s):  
Platelet Aggregation ◽  
The Other ◽  
Platelet Inhibitors ◽  
Platelet Dysfunction ◽  
Self Medication ◽  
History Of ◽  
Platelet Aggregates ◽  
The Mean ◽  
Normal Controls ◽  
Age And Sex

SummaryCirculating microembolic index (CMI) was determined by drawing one blood sample into EDTA-formalin and the other into DTA alone in patients with migraine and compared with matched normal controls. Platelet aggregates, if any, are fixed in EDTA-formalin but dis- aggregated by EDTA. Ratios of these two counts approximate “unity” in normals and are proportionately less than unity, depending on the number of platelet aggregates. 26 untreated migraineurs and 19 migraineurs with history of self-medication with aspirin taken within 72 hours of the test, were studied in headache-free intervals. Results were compared with those from 20 healthy, age and sex matched volunteers, without migraine, who were medication- free for at least one week. Mean CMI in untreated migraineurs (0.77±0.03 SEM) was significantly lower than the mean in normal controls (0.94±0.02, p. <0.002). Migraineurs with selfadministration of aspirin had mean CMI of 0.88±0.02, differing significantly from untreated migraineurs (p <0.01) but not from normal controls (0.1<p<0.2). Results suggest excessive platelet aggregation in migraineurs which tends to be corrected by treatment with platelet inhibitors such as aspirin.

scholarly journals Sheep pestivirus in Morocco: sero-epidemiological and molecular study

2019 ◽  
Vol 6 (1) ◽  
pp. e000324 ◽  
Author(s):  
Ouafaa Fassi Fihri ◽  
Noâma Jammar ◽  
Nadia Amrani ◽  
Ikhlass El Berbri ◽  
Said Alali
Keyword(s):  
Reverse Transcriptase ◽  
Indirect Elisa ◽  
Molecular Study ◽  
Rt Pcr ◽  
Blood Samples ◽  
Sheep Breeding ◽  
Border Disease ◽  
Elisa Technique ◽  
The Difference

The present study is the first to investigate Border disease caused by the sheep pestivirus (SPV) in sheep herds in Morocco. Sero-epidemiological investigations were carried out in six regions of the Kingdom, known as important in terms of sheep breeding. A total of 760 blood samples were collected including aborted ewes from 28 randomly selected farms. The samples were analysed, for the determination of anti-pestivirus antibodies, using indirect ELISA technique. Next, reverse transcriptase PCR (RT-PCR) was conducted on serologically negative samples to identify possible persistently infected (PI) animals, through detection of specific RNA fragment. The results revealed an overall SPV seroprevalence in studied areas of 28.9%. The difference in seroprevalence between the six investigated regions was not statistically significant (p>0.05) and varied slightly from 20.9% to 37.5%. Furthermore, 93% of investigated farms were affected with an average seroprevalence of 22.7% (with a variation of 1%–74%). RT-PCR results were all negative, indicating the absence of PI animals in the tested samples. Nevertheless, the present study revealed that SPV is endemic in Morocco.

scholarly journals Toxoplasma gondii: infection among shelter and stray cats in Rio de Janeiro, Brazil

2018 ◽  
Author(s):  
Pâmela Figueiredo Pereira ◽  
Alynne da Silva Barbosa ◽  
Ana Leticia Carvalho Santos ◽  
Paula Forain Bolais ◽  
Marie-Laure Dardé ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rio De Janeiro ◽  
The Other ◽  
Hemagglutination Assay ◽  
Blood Samples ◽  
Stray Cats ◽  
Serological Status ◽  
Indirect Hemagglutination ◽  
Toxoplasma Gondii Infection ◽  
The City

Abstract The aim of this study was to identify possible infection of Toxoplasma gondii among cats in a shelter and a set of condominiums in the city of Rio de Janeiro, through changes to the cats’ serological status between two different times in 2014 and 2015. One group was made up of captive cats at the municipal shelter and the other comprised stray cats that circulated in condominiums in the city. On the first occasion, cats were caught and tagged through application of microchips; in this manner, blood samples were obtained from 261 captive cats and 172 stray cats. On the second occasion, blood samples were obtained from 94 captive cats and 56 recaptured stray cats. The serological diagnosis was made by means of the indirect hemagglutination assay (IHA) and indirect immunofluorescence reaction (IFAT) (cutoff ≥ 64). The frequency of T. gondii infection among the captive cats was 24.5% and among the stray cats, 18%. With the second analysis, it was possible to verify modifications to the serological status of anti-T. gondii antibodies, in 18% of both populations of animals. The presence of seroconversion shows that infection was possibly occurring in the region at the time of the study.

scholarly journals Molecular Detection of Toxoplasma Gondii in Haemaphysalis Ticks in Korea

2020 ◽  
Vol 58 (3) ◽  
pp. 327-331
Author(s):  
Ju Yeong Kim ◽  
You Shine Kwak ◽  
In-Yong Lee ◽  
Tai-Soon Yong
Keyword(s):  
Toxoplasma Gondii ◽  
Neurological Disease ◽  
Tick Species ◽  
Molecular Detection ◽  
Development Stage ◽  
Haemaphysalis Longicornis ◽  
First Report ◽  
B1 Gene ◽  
Haemaphysalis Flava ◽  
Human Adults

Toxoplasma gondii are intracellular protozoa that can cause neurological disease or death in fetuses and even in immunocompromised human adults. Ticks are recognized as vectors of many microorganisms including viruses, bacteria, and protozoa. Recent studies detected T. gondii in various tick species in many countries. In this study, we performed PCR detection of the T. gondii B1 gene from Haemaphysalis ticks collected from vegetation in 4 localities, Wonju, Gunsan, Miryang, and Yangsan, in Korea. We analyzed DNA from 314 ticks (268 Haemaphysalis longicornis and 46 Haemaphysalis flava) and the B1 gene of T. gondii was detected in 13 of these. The detection of T. gondii in ticks differed significantly by region (P=0.021). T. gondii was detected in the following percentages of collected ticks: 3.7% (7 of 189) in Gunsan, 10% (5 of 50) in Wonju, 16.7% (1 of 6) in Yangsan, and 0% (0 of 69) in Miryang. The detection of T. gondii in ticks was not associated with tick species or development stage. This is the first report of T. gondii detection in ticks in Korea. Our results provide important information necessary to understand toxoplasmosis transmission.

scholarly journals PCR detection of Toxoplasma gondii B1 gene in women suffering from abortion

2019 ◽  
Vol 13 (1) ◽  
pp. 12-15
Author(s):  
Aseel S. Mahmood ◽  
Sabeeha A. Al-Sarray ◽  
Abdulkareem Al-Kazaz
Keyword(s):  
Toxoplasma Gondii ◽  
Complex Disease ◽  
Control Group ◽  
Chi Square ◽  
Nested Polymerase Chain Reaction ◽  
B1 Gene ◽  
History Of ◽  
Polymerase Chain ◽  
And Control ◽  
Apparently Healthy

Background: Primary infection of maternal with toxoplasmosis during gestation and this infection transmission to the fetus continue to be the cause complex disease in offspring. Objective: This study was conducted to test the utility of nested Polymerase Chain Reaction (nPCR) assay to detect recent infections with Toxoplasma in abortive women. Material and methods: Toxoplasma gondii DNA was detected by using B1 gene as a target for amplification which was highly specific for T. gondii and is well conserved among all of the tested strains. Blood from 60 abortive women and 25 apparently healthy pregnant women with no history of abortion (as control group) were taken in this current study. Results: The results revealed that nPCR was positive in 48(80%) subjects and negative in 12(20%), Chi-square- χ2 for patients and control was ( 13.82 , 15.75 ) respectively. Conclusion: It can be concluded that nPCR assay in blood has advantage in detection of recent and active toxoplasmosis.

scholarly journals Screening for thalassaemia among group of students of a higher institution – our experience

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Norlelawati AT ◽  
Siti Hadijah M ◽  
Siti Nor Haiza H ◽  
Rusmawati I ◽  
Salman MS ◽  
...  
Keyword(s):  
Family History ◽  
Molecular Study ◽  
Full Blood Count ◽  
Blood Samples ◽  
Blood Disorder ◽  
Higher Institution ◽  
Haemoglobin Disorders ◽  
Thalassaemia Trait ◽  
History Of ◽  
Alpha Thalassaemia

Introduction: Thalassaemia is an inherited blood disorder and is a significant public health alarm in Malaysia with many not knowing they are carriers of this haemoglobin disorders.  Materials and methods: This study conducted a one off collection of blood samples from 72 Malays students of International Islamic University Malaysia (IIUM) in Kuantan.  Blood samples were subjected to conventional haemoglobin analyses that include full blood count and picture, HPLC, Haemoglobin electrophoresis and H-inclusion test. All samples were also genotyped for alpha thalassaemia–1 of Southeast Asia (a-Thal1SEA).  Result: There were 17(23.6%) students who were diagnosed as thalassaemia carriers.  Out of this, four (5.5 %) and six (8.3 %) students were presumptive β-thalassaemia trait and Haemoglobin-E trait as determined by the HPLC assay respectively.  Nine (12.5%) students were genotyped a-Thal1SEA among whom two were also β-thalassaemia carriers.  All thalassaemia cases had MCH of < 27pg.  Nonetheless, two out of six Haemoglobin-E trait and three out of nine a-Thal1SEA carrier had MCV value of >80fL. Two out of four (50%) presumptive β -thalassaemia trait and one out of six (17%) students of presumptive Haemoglobin-E trait had family history of thalassaemia respectively. Conclusion: The high occurrence of the three common types of thalassaemia carrier (β, Hb-E and a-Thal1SEA thalassaemia) in our small group of subjects could be due to better participation of students who had family history of thalassaemia.  The study reaffirmed the importance of molecular study for detection of alpha-thalassaemia and the use of MCH value of <27pg rather than MCV value of < 80fL for prediction of thalassaemia.

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